Job ID = 6367405 SRX = SRX331164 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:31:35 prefetch.2.10.7: 1) Downloading 'SRR947396'... 2020-06-15T23:31:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:32:01 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:32:01 prefetch.2.10.7: 'SRR947396' is valid 2020-06-15T23:32:01 prefetch.2.10.7: 1) 'SRR947396' was downloaded successfully Read 3204493 spots for SRR947396/SRR947396.sra Written 3204493 spots for SRR947396/SRR947396.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:26 3204493 reads; of these: 3204493 (100.00%) were unpaired; of these: 797049 (24.87%) aligned 0 times 2006760 (62.62%) aligned exactly 1 time 400684 (12.50%) aligned >1 times 75.13% overall alignment rate Time searching: 00:00:26 Overall time: 00:00:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 416292 / 2407444 = 0.1729 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:39: 1000000 INFO @ Tue, 16 Jun 2020 08:33:45: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:33:45: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:33:45: #1 total tags in treatment: 1991152 INFO @ Tue, 16 Jun 2020 08:33:45: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:45: #1 tags after filtering in treatment: 1991152 INFO @ Tue, 16 Jun 2020 08:33:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:45: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:45: #2 number of paired peaks: 1145 INFO @ Tue, 16 Jun 2020 08:33:45: start model_add_line... INFO @ Tue, 16 Jun 2020 08:33:45: start X-correlation... INFO @ Tue, 16 Jun 2020 08:33:45: end of X-cor INFO @ Tue, 16 Jun 2020 08:33:45: #2 finished! INFO @ Tue, 16 Jun 2020 08:33:45: #2 predicted fragment length is 157 bps INFO @ Tue, 16 Jun 2020 08:33:45: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 16 Jun 2020 08:33:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.05_model.r INFO @ Tue, 16 Jun 2020 08:33:45: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:33:45: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:33:50: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:33:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:33:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:33:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.05_summits.bed INFO @ Tue, 16 Jun 2020 08:33:52: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1862 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:07: 1000000 INFO @ Tue, 16 Jun 2020 08:34:12: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:34:12: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:34:12: #1 total tags in treatment: 1991152 INFO @ Tue, 16 Jun 2020 08:34:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:12: #1 tags after filtering in treatment: 1991152 INFO @ Tue, 16 Jun 2020 08:34:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:12: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:12: #2 number of paired peaks: 1145 INFO @ Tue, 16 Jun 2020 08:34:12: start model_add_line... INFO @ Tue, 16 Jun 2020 08:34:12: start X-correlation... INFO @ Tue, 16 Jun 2020 08:34:12: end of X-cor INFO @ Tue, 16 Jun 2020 08:34:12: #2 finished! INFO @ Tue, 16 Jun 2020 08:34:12: #2 predicted fragment length is 157 bps INFO @ Tue, 16 Jun 2020 08:34:12: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 16 Jun 2020 08:34:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.10_model.r INFO @ Tue, 16 Jun 2020 08:34:12: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:34:12: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:34:18: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:34:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:34:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.10_summits.bed INFO @ Tue, 16 Jun 2020 08:34:20: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (977 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:38: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:34:44: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:34:44: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:34:44: #1 total tags in treatment: 1991152 INFO @ Tue, 16 Jun 2020 08:34:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:34:44: #1 tags after filtering in treatment: 1991152 INFO @ Tue, 16 Jun 2020 08:34:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:44: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:45: #2 number of paired peaks: 1145 INFO @ Tue, 16 Jun 2020 08:34:45: start model_add_line... INFO @ Tue, 16 Jun 2020 08:34:45: start X-correlation... INFO @ Tue, 16 Jun 2020 08:34:45: end of X-cor INFO @ Tue, 16 Jun 2020 08:34:45: #2 finished! INFO @ Tue, 16 Jun 2020 08:34:45: #2 predicted fragment length is 157 bps INFO @ Tue, 16 Jun 2020 08:34:45: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 16 Jun 2020 08:34:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.20_model.r INFO @ Tue, 16 Jun 2020 08:34:45: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:34:45: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:34:50: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:34:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:34:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:34:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331164/SRX331164.20_summits.bed INFO @ Tue, 16 Jun 2020 08:34:52: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (474 records, 4 fields): 1 millis CompletedMACS2peakCalling