Job ID = 6367385 SRX = SRX331144 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:34:35 prefetch.2.10.7: 1) Downloading 'SRR947375'... 2020-06-15T23:34:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:35:38 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:35:38 prefetch.2.10.7: 'SRR947375' is valid 2020-06-15T23:35:38 prefetch.2.10.7: 1) 'SRR947375' was downloaded successfully Read 5840271 spots for SRR947375/SRR947375.sra Written 5840271 spots for SRR947375/SRR947375.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:41 5840271 reads; of these: 5840271 (100.00%) were unpaired; of these: 2911028 (49.84%) aligned 0 times 2414808 (41.35%) aligned exactly 1 time 514435 (8.81%) aligned >1 times 50.16% overall alignment rate Time searching: 00:00:41 Overall time: 00:00:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 140919 / 2929243 = 0.0481 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:37:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:37:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:37:48: 1000000 INFO @ Tue, 16 Jun 2020 08:37:55: 2000000 INFO @ Tue, 16 Jun 2020 08:38:00: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:38:00: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:38:00: #1 total tags in treatment: 2788324 INFO @ Tue, 16 Jun 2020 08:38:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:00: #1 tags after filtering in treatment: 2788324 INFO @ Tue, 16 Jun 2020 08:38:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:00: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:00: #2 number of paired peaks: 411 WARNING @ Tue, 16 Jun 2020 08:38:00: Fewer paired peaks (411) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 411 pairs to build model! INFO @ Tue, 16 Jun 2020 08:38:00: start model_add_line... INFO @ Tue, 16 Jun 2020 08:38:00: start X-correlation... INFO @ Tue, 16 Jun 2020 08:38:00: end of X-cor INFO @ Tue, 16 Jun 2020 08:38:00: #2 finished! INFO @ Tue, 16 Jun 2020 08:38:00: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:38:00: #2 alternative fragment length(s) may be 34,490,572 bps INFO @ Tue, 16 Jun 2020 08:38:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.05_model.r WARNING @ Tue, 16 Jun 2020 08:38:00: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:38:00: #2 You may need to consider one of the other alternative d(s): 34,490,572 WARNING @ Tue, 16 Jun 2020 08:38:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:38:00: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:38:00: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:38:06: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:38:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:38:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:38:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.05_summits.bed INFO @ Tue, 16 Jun 2020 08:38:09: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (332 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:38:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:38:11: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:38:11: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:38:17: 1000000 INFO @ Tue, 16 Jun 2020 08:38:22: 2000000 INFO @ Tue, 16 Jun 2020 08:38:26: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:38:26: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:38:26: #1 total tags in treatment: 2788324 INFO @ Tue, 16 Jun 2020 08:38:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:26: #1 tags after filtering in treatment: 2788324 INFO @ Tue, 16 Jun 2020 08:38:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:26: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:27: #2 number of paired peaks: 411 WARNING @ Tue, 16 Jun 2020 08:38:27: Fewer paired peaks (411) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 411 pairs to build model! INFO @ Tue, 16 Jun 2020 08:38:27: start model_add_line... INFO @ Tue, 16 Jun 2020 08:38:27: start X-correlation... INFO @ Tue, 16 Jun 2020 08:38:27: end of X-cor INFO @ Tue, 16 Jun 2020 08:38:27: #2 finished! INFO @ Tue, 16 Jun 2020 08:38:27: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:38:27: #2 alternative fragment length(s) may be 34,490,572 bps INFO @ Tue, 16 Jun 2020 08:38:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.10_model.r WARNING @ Tue, 16 Jun 2020 08:38:27: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:38:27: #2 You may need to consider one of the other alternative d(s): 34,490,572 WARNING @ Tue, 16 Jun 2020 08:38:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:38:27: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:38:27: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:38:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:38:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:38:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:38:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.10_summits.bed INFO @ Tue, 16 Jun 2020 08:38:35: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (166 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:38:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:38:41: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:38:41: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:38:47: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:38:52: 2000000 INFO @ Tue, 16 Jun 2020 08:38:57: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:38:57: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:38:57: #1 total tags in treatment: 2788324 INFO @ Tue, 16 Jun 2020 08:38:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:38:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:38:57: #1 tags after filtering in treatment: 2788324 INFO @ Tue, 16 Jun 2020 08:38:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:38:57: #1 finished! INFO @ Tue, 16 Jun 2020 08:38:57: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:38:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:38:57: #2 number of paired peaks: 411 WARNING @ Tue, 16 Jun 2020 08:38:57: Fewer paired peaks (411) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 411 pairs to build model! INFO @ Tue, 16 Jun 2020 08:38:57: start model_add_line... INFO @ Tue, 16 Jun 2020 08:38:57: start X-correlation... INFO @ Tue, 16 Jun 2020 08:38:57: end of X-cor INFO @ Tue, 16 Jun 2020 08:38:57: #2 finished! INFO @ Tue, 16 Jun 2020 08:38:57: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:38:57: #2 alternative fragment length(s) may be 34,490,572 bps INFO @ Tue, 16 Jun 2020 08:38:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.20_model.r WARNING @ Tue, 16 Jun 2020 08:38:57: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:38:57: #2 You may need to consider one of the other alternative d(s): 34,490,572 WARNING @ Tue, 16 Jun 2020 08:38:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:38:57: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:38:57: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:39:03: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:39:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:39:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:39:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331144/SRX331144.20_summits.bed INFO @ Tue, 16 Jun 2020 08:39:06: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (37 records, 4 fields): 1 millis CompletedMACS2peakCalling