Job ID = 6367324 SRX = SRX331086 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:23:16 prefetch.2.10.7: 1) Downloading 'SRR947314'... 2020-06-15T23:23:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:23:33 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:23:33 prefetch.2.10.7: 'SRR947314' is valid 2020-06-15T23:23:33 prefetch.2.10.7: 1) 'SRR947314' was downloaded successfully Read 1573914 spots for SRR947314/SRR947314.sra Written 1573914 spots for SRR947314/SRR947314.sra 2020-06-15T23:23:48 prefetch.2.10.7: 1) Downloading 'SRR947315'... 2020-06-15T23:23:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:24:12 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:24:12 prefetch.2.10.7: 'SRR947315' is valid 2020-06-15T23:24:12 prefetch.2.10.7: 1) 'SRR947315' was downloaded successfully Read 2502361 spots for SRR947315/SRR947315.sra Written 2502361 spots for SRR947315/SRR947315.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:31 4076275 reads; of these: 4076275 (100.00%) were unpaired; of these: 1610714 (39.51%) aligned 0 times 2095452 (51.41%) aligned exactly 1 time 370109 (9.08%) aligned >1 times 60.49% overall alignment rate Time searching: 00:00:31 Overall time: 00:00:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1468172 / 2465561 = 0.5955 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:25:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:25:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:25:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:25:50: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:25:50: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:25:50: #1 total tags in treatment: 997389 INFO @ Tue, 16 Jun 2020 08:25:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:25:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:25:50: #1 tags after filtering in treatment: 997389 INFO @ Tue, 16 Jun 2020 08:25:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:25:50: #1 finished! INFO @ Tue, 16 Jun 2020 08:25:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:25:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:25:50: #2 number of paired peaks: 1833 INFO @ Tue, 16 Jun 2020 08:25:50: start model_add_line... INFO @ Tue, 16 Jun 2020 08:25:50: start X-correlation... INFO @ Tue, 16 Jun 2020 08:25:50: end of X-cor INFO @ Tue, 16 Jun 2020 08:25:50: #2 finished! INFO @ Tue, 16 Jun 2020 08:25:50: #2 predicted fragment length is 121 bps INFO @ Tue, 16 Jun 2020 08:25:50: #2 alternative fragment length(s) may be 121 bps INFO @ Tue, 16 Jun 2020 08:25:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.05_model.r INFO @ Tue, 16 Jun 2020 08:25:50: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:25:50: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:25:52: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:25:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:25:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:25:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.05_summits.bed INFO @ Tue, 16 Jun 2020 08:25:54: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1954 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:26:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:26:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:26:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:26:19: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:26:19: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:26:19: #1 total tags in treatment: 997389 INFO @ Tue, 16 Jun 2020 08:26:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:26:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:26:19: #1 tags after filtering in treatment: 997389 INFO @ Tue, 16 Jun 2020 08:26:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:26:19: #1 finished! INFO @ Tue, 16 Jun 2020 08:26:19: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:26:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:26:19: #2 number of paired peaks: 1833 INFO @ Tue, 16 Jun 2020 08:26:19: start model_add_line... INFO @ Tue, 16 Jun 2020 08:26:19: start X-correlation... INFO @ Tue, 16 Jun 2020 08:26:19: end of X-cor INFO @ Tue, 16 Jun 2020 08:26:19: #2 finished! INFO @ Tue, 16 Jun 2020 08:26:19: #2 predicted fragment length is 121 bps INFO @ Tue, 16 Jun 2020 08:26:19: #2 alternative fragment length(s) may be 121 bps INFO @ Tue, 16 Jun 2020 08:26:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.10_model.r INFO @ Tue, 16 Jun 2020 08:26:19: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:26:19: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:26:21: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:26:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:26:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:26:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.10_summits.bed INFO @ Tue, 16 Jun 2020 08:26:22: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (1069 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:26:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:26:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:26:44: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:26:49: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:26:49: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:26:49: #1 total tags in treatment: 997389 INFO @ Tue, 16 Jun 2020 08:26:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:26:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:26:49: #1 tags after filtering in treatment: 997389 INFO @ Tue, 16 Jun 2020 08:26:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:26:49: #1 finished! INFO @ Tue, 16 Jun 2020 08:26:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:26:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:26:49: #2 number of paired peaks: 1833 INFO @ Tue, 16 Jun 2020 08:26:49: start model_add_line... INFO @ Tue, 16 Jun 2020 08:26:49: start X-correlation... INFO @ Tue, 16 Jun 2020 08:26:49: end of X-cor INFO @ Tue, 16 Jun 2020 08:26:49: #2 finished! INFO @ Tue, 16 Jun 2020 08:26:49: #2 predicted fragment length is 121 bps INFO @ Tue, 16 Jun 2020 08:26:49: #2 alternative fragment length(s) may be 121 bps INFO @ Tue, 16 Jun 2020 08:26:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.20_model.r INFO @ Tue, 16 Jun 2020 08:26:49: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:26:49: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:26:52: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:26:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:26:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:26:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX331086/SRX331086.20_summits.bed INFO @ Tue, 16 Jun 2020 08:26:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (513 records, 4 fields): 1 millis CompletedMACS2peakCalling