Job ID = 6367270 SRX = SRX323691 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:23:01 prefetch.2.10.7: 1) Downloading 'SRR935766'... 2020-06-15T23:23:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:24:15 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:24:15 prefetch.2.10.7: 1) 'SRR935766' was downloaded successfully Read 16048349 spots for SRR935766/SRR935766.sra Written 16048349 spots for SRR935766/SRR935766.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:17 16048349 reads; of these: 16048349 (100.00%) were unpaired; of these: 1679273 (10.46%) aligned 0 times 12470661 (77.71%) aligned exactly 1 time 1898415 (11.83%) aligned >1 times 89.54% overall alignment rate Time searching: 00:04:17 Overall time: 00:04:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3936559 / 14369076 = 0.2740 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:38: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:38: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:44: 1000000 INFO @ Tue, 16 Jun 2020 08:33:51: 2000000 INFO @ Tue, 16 Jun 2020 08:33:57: 3000000 INFO @ Tue, 16 Jun 2020 08:34:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:08: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:08: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:10: 5000000 INFO @ Tue, 16 Jun 2020 08:34:16: 1000000 INFO @ Tue, 16 Jun 2020 08:34:18: 6000000 INFO @ Tue, 16 Jun 2020 08:34:25: 2000000 INFO @ Tue, 16 Jun 2020 08:34:25: 7000000 INFO @ Tue, 16 Jun 2020 08:34:33: 8000000 INFO @ Tue, 16 Jun 2020 08:34:34: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:34:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:34:38: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:34:38: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:34:41: 9000000 INFO @ Tue, 16 Jun 2020 08:34:43: 4000000 INFO @ Tue, 16 Jun 2020 08:34:46: 1000000 INFO @ Tue, 16 Jun 2020 08:34:50: 10000000 INFO @ Tue, 16 Jun 2020 08:34:52: 5000000 INFO @ Tue, 16 Jun 2020 08:34:53: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:34:53: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:34:53: #1 total tags in treatment: 10432517 INFO @ Tue, 16 Jun 2020 08:34:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:53: #1 tags after filtering in treatment: 10432517 INFO @ Tue, 16 Jun 2020 08:34:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:53: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:54: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 08:34:54: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:34:54: Process for pairing-model is terminated! INFO @ Tue, 16 Jun 2020 08:34:54: 2000000 cut: /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:35:01: 6000000 INFO @ Tue, 16 Jun 2020 08:35:02: 3000000 INFO @ Tue, 16 Jun 2020 08:35:10: 7000000 INFO @ Tue, 16 Jun 2020 08:35:10: 4000000 INFO @ Tue, 16 Jun 2020 08:35:18: 5000000 INFO @ Tue, 16 Jun 2020 08:35:18: 8000000 INFO @ Tue, 16 Jun 2020 08:35:26: 6000000 INFO @ Tue, 16 Jun 2020 08:35:27: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:35:34: 7000000 INFO @ Tue, 16 Jun 2020 08:35:35: 10000000 INFO @ Tue, 16 Jun 2020 08:35:39: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:35:39: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:35:39: #1 total tags in treatment: 10432517 INFO @ Tue, 16 Jun 2020 08:35:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:35:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:35:39: #1 tags after filtering in treatment: 10432517 INFO @ Tue, 16 Jun 2020 08:35:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:35:39: #1 finished! INFO @ Tue, 16 Jun 2020 08:35:39: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:35:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:35:40: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 08:35:40: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:35:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:35:42: 8000000 INFO @ Tue, 16 Jun 2020 08:35:48: 9000000 INFO @ Tue, 16 Jun 2020 08:35:55: 10000000 INFO @ Tue, 16 Jun 2020 08:35:58: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:35:58: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:35:58: #1 total tags in treatment: 10432517 INFO @ Tue, 16 Jun 2020 08:35:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:35:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:35:58: #1 tags after filtering in treatment: 10432517 INFO @ Tue, 16 Jun 2020 08:35:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:35:58: #1 finished! INFO @ Tue, 16 Jun 2020 08:35:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:35:58: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:35:58: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 08:35:58: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:35:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323691/SRX323691.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling