Job ID = 6367267 SRX = SRX323688 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:20:01 prefetch.2.10.7: 1) Downloading 'SRR935762'... 2020-06-15T23:20:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:21:35 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:21:35 prefetch.2.10.7: 1) 'SRR935762' was downloaded successfully Read 19710753 spots for SRR935762/SRR935762.sra Written 19710753 spots for SRR935762/SRR935762.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 19710753 reads; of these: 19710753 (100.00%) were unpaired; of these: 2858589 (14.50%) aligned 0 times 14445047 (73.29%) aligned exactly 1 time 2407117 (12.21%) aligned >1 times 85.50% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6703084 / 16852164 = 0.3978 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:14: 1000000 INFO @ Tue, 16 Jun 2020 08:32:19: 2000000 INFO @ Tue, 16 Jun 2020 08:32:24: 3000000 INFO @ Tue, 16 Jun 2020 08:32:29: 4000000 INFO @ Tue, 16 Jun 2020 08:32:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:40: 6000000 INFO @ Tue, 16 Jun 2020 08:32:44: 1000000 INFO @ Tue, 16 Jun 2020 08:32:45: 7000000 INFO @ Tue, 16 Jun 2020 08:32:49: 2000000 INFO @ Tue, 16 Jun 2020 08:32:51: 8000000 INFO @ Tue, 16 Jun 2020 08:32:55: 3000000 INFO @ Tue, 16 Jun 2020 08:32:56: 9000000 INFO @ Tue, 16 Jun 2020 08:33:00: 4000000 INFO @ Tue, 16 Jun 2020 08:33:02: 10000000 INFO @ Tue, 16 Jun 2020 08:33:02: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:33:02: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:33:02: #1 total tags in treatment: 10149080 INFO @ Tue, 16 Jun 2020 08:33:02: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:03: #1 tags after filtering in treatment: 10149080 INFO @ Tue, 16 Jun 2020 08:33:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:03: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:03: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 08:33:03: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:33:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:33:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:08: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:08: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:33:11: 6000000 INFO @ Tue, 16 Jun 2020 08:33:14: 1000000 INFO @ Tue, 16 Jun 2020 08:33:17: 7000000 INFO @ Tue, 16 Jun 2020 08:33:19: 2000000 INFO @ Tue, 16 Jun 2020 08:33:22: 8000000 INFO @ Tue, 16 Jun 2020 08:33:25: 3000000 INFO @ Tue, 16 Jun 2020 08:33:28: 9000000 INFO @ Tue, 16 Jun 2020 08:33:30: 4000000 INFO @ Tue, 16 Jun 2020 08:33:33: 10000000 INFO @ Tue, 16 Jun 2020 08:33:34: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:33:34: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:33:34: #1 total tags in treatment: 10149080 INFO @ Tue, 16 Jun 2020 08:33:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:34: #1 tags after filtering in treatment: 10149080 INFO @ Tue, 16 Jun 2020 08:33:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:34: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:35: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 08:33:35: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:33:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:33:36: 5000000 INFO @ Tue, 16 Jun 2020 08:33:41: 6000000 INFO @ Tue, 16 Jun 2020 08:33:46: 7000000 INFO @ Tue, 16 Jun 2020 08:33:52: 8000000 INFO @ Tue, 16 Jun 2020 08:33:57: 9000000 INFO @ Tue, 16 Jun 2020 08:34:02: 10000000 INFO @ Tue, 16 Jun 2020 08:34:03: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:34:03: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:34:03: #1 total tags in treatment: 10149080 INFO @ Tue, 16 Jun 2020 08:34:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:34:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:34:03: #1 tags after filtering in treatment: 10149080 INFO @ Tue, 16 Jun 2020 08:34:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:34:03: #1 finished! INFO @ Tue, 16 Jun 2020 08:34:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:34:04: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 08:34:04: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:34:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323688/SRX323688.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。