Job ID = 6367262 SRX = SRX323683 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:23:01 prefetch.2.10.7: 1) Downloading 'SRR935757'... 2020-06-15T23:23:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:24:08 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:24:09 prefetch.2.10.7: 'SRR935757' is valid 2020-06-15T23:24:09 prefetch.2.10.7: 1) 'SRR935757' was downloaded successfully Read 13687820 spots for SRR935757/SRR935757.sra Written 13687820 spots for SRR935757/SRR935757.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:25 13687820 reads; of these: 13687820 (100.00%) were unpaired; of these: 1668659 (12.19%) aligned 0 times 10704639 (78.21%) aligned exactly 1 time 1314522 (9.60%) aligned >1 times 87.81% overall alignment rate Time searching: 00:03:25 Overall time: 00:03:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3793304 / 12019161 = 0.3156 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:31:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:31:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:31:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:31:51: 1000000 INFO @ Tue, 16 Jun 2020 08:31:59: 2000000 INFO @ Tue, 16 Jun 2020 08:32:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:15: 4000000 INFO @ Tue, 16 Jun 2020 08:32:21: 1000000 INFO @ Tue, 16 Jun 2020 08:32:23: 5000000 INFO @ Tue, 16 Jun 2020 08:32:28: 2000000 INFO @ Tue, 16 Jun 2020 08:32:32: 6000000 INFO @ Tue, 16 Jun 2020 08:32:36: 3000000 INFO @ Tue, 16 Jun 2020 08:32:40: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:32:43: 4000000 INFO @ Tue, 16 Jun 2020 08:32:49: 8000000 INFO @ Tue, 16 Jun 2020 08:32:51: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:32:51: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:32:51: #1 total tags in treatment: 8225857 INFO @ Tue, 16 Jun 2020 08:32:51: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:32:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:32:51: #1 tags after filtering in treatment: 8225857 INFO @ Tue, 16 Jun 2020 08:32:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:32:51: #1 finished! INFO @ Tue, 16 Jun 2020 08:32:51: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:32:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:32:51: 5000000 INFO @ Tue, 16 Jun 2020 08:32:52: 1000000 INFO @ Tue, 16 Jun 2020 08:32:52: #2 number of paired peaks: 60 WARNING @ Tue, 16 Jun 2020 08:32:52: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:32:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:32:59: 6000000 INFO @ Tue, 16 Jun 2020 08:33:00: 2000000 INFO @ Tue, 16 Jun 2020 08:33:06: 7000000 INFO @ Tue, 16 Jun 2020 08:33:09: 3000000 INFO @ Tue, 16 Jun 2020 08:33:14: 8000000 INFO @ Tue, 16 Jun 2020 08:33:15: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:33:15: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:33:15: #1 total tags in treatment: 8225857 INFO @ Tue, 16 Jun 2020 08:33:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:16: #1 tags after filtering in treatment: 8225857 INFO @ Tue, 16 Jun 2020 08:33:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:16: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:16: #2 number of paired peaks: 60 WARNING @ Tue, 16 Jun 2020 08:33:16: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:33:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:33:17: 4000000 INFO @ Tue, 16 Jun 2020 08:33:25: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:33:33: 6000000 INFO @ Tue, 16 Jun 2020 08:33:40: 7000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:33:48: 8000000 INFO @ Tue, 16 Jun 2020 08:33:50: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:33:50: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:33:50: #1 total tags in treatment: 8225857 INFO @ Tue, 16 Jun 2020 08:33:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:50: #1 tags after filtering in treatment: 8225857 INFO @ Tue, 16 Jun 2020 08:33:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:33:50: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:50: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:51: #2 number of paired peaks: 60 WARNING @ Tue, 16 Jun 2020 08:33:51: Too few paired peaks (60) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:33:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323683/SRX323683.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling