Job ID = 6367258 SRX = SRX323679 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:17:01 prefetch.2.10.7: 1) Downloading 'SRR935753'... 2020-06-15T23:17:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:18:13 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:18:13 prefetch.2.10.7: 1) 'SRR935753' was downloaded successfully Read 15072086 spots for SRR935753/SRR935753.sra Written 15072086 spots for SRR935753/SRR935753.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:02 15072086 reads; of these: 15072086 (100.00%) were unpaired; of these: 835785 (5.55%) aligned 0 times 12131798 (80.49%) aligned exactly 1 time 2104503 (13.96%) aligned >1 times 94.45% overall alignment rate Time searching: 00:04:02 Overall time: 00:04:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3509451 / 14236301 = 0.2465 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:27:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:27:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:27:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:27:10: 1000000 INFO @ Tue, 16 Jun 2020 08:27:17: 2000000 INFO @ Tue, 16 Jun 2020 08:27:24: 3000000 INFO @ Tue, 16 Jun 2020 08:27:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:27:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:27:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:27:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:27:37: 5000000 INFO @ Tue, 16 Jun 2020 08:27:39: 1000000 INFO @ Tue, 16 Jun 2020 08:27:44: 6000000 INFO @ Tue, 16 Jun 2020 08:27:46: 2000000 INFO @ Tue, 16 Jun 2020 08:27:51: 7000000 INFO @ Tue, 16 Jun 2020 08:27:53: 3000000 INFO @ Tue, 16 Jun 2020 08:27:58: 8000000 INFO @ Tue, 16 Jun 2020 08:27:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:28:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:28:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:28:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:28:04: 9000000 INFO @ Tue, 16 Jun 2020 08:28:06: 5000000 INFO @ Tue, 16 Jun 2020 08:28:10: 1000000 INFO @ Tue, 16 Jun 2020 08:28:12: 10000000 INFO @ Tue, 16 Jun 2020 08:28:14: 6000000 INFO @ Tue, 16 Jun 2020 08:28:17: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:28:17: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:28:17: #1 total tags in treatment: 10726850 INFO @ Tue, 16 Jun 2020 08:28:17: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:28:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:28:17: #1 tags after filtering in treatment: 10726850 INFO @ Tue, 16 Jun 2020 08:28:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:28:17: #1 finished! INFO @ Tue, 16 Jun 2020 08:28:17: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:28:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:28:17: 2000000 INFO @ Tue, 16 Jun 2020 08:28:18: #2 number of paired peaks: 42 WARNING @ Tue, 16 Jun 2020 08:28:18: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:28:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:28:21: 7000000 INFO @ Tue, 16 Jun 2020 08:28:24: 3000000 INFO @ Tue, 16 Jun 2020 08:28:28: 8000000 INFO @ Tue, 16 Jun 2020 08:28:31: 4000000 INFO @ Tue, 16 Jun 2020 08:28:35: 9000000 INFO @ Tue, 16 Jun 2020 08:28:38: 5000000 INFO @ Tue, 16 Jun 2020 08:28:42: 10000000 INFO @ Tue, 16 Jun 2020 08:28:45: 6000000 INFO @ Tue, 16 Jun 2020 08:28:47: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:28:47: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:28:47: #1 total tags in treatment: 10726850 INFO @ Tue, 16 Jun 2020 08:28:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:28:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:28:47: #1 tags after filtering in treatment: 10726850 INFO @ Tue, 16 Jun 2020 08:28:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:28:47: #1 finished! INFO @ Tue, 16 Jun 2020 08:28:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:28:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:28:48: #2 number of paired peaks: 42 WARNING @ Tue, 16 Jun 2020 08:28:48: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:28:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:28:51: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:28:58: 8000000 INFO @ Tue, 16 Jun 2020 08:29:04: 9000000 INFO @ Tue, 16 Jun 2020 08:29:10: 10000000 INFO @ Tue, 16 Jun 2020 08:29:14: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:29:14: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:29:14: #1 total tags in treatment: 10726850 INFO @ Tue, 16 Jun 2020 08:29:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:29:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:29:15: #1 tags after filtering in treatment: 10726850 INFO @ Tue, 16 Jun 2020 08:29:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:29:15: #1 finished! INFO @ Tue, 16 Jun 2020 08:29:15: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:29:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:29:15: #2 number of paired peaks: 42 WARNING @ Tue, 16 Jun 2020 08:29:15: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:29:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX323679/SRX323679.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。