Job ID = 14159612 SRX = SRX3180835 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:20 49165892 reads; of these: 49165892 (100.00%) were unpaired; of these: 1267118 (2.58%) aligned 0 times 39824161 (81.00%) aligned exactly 1 time 8074613 (16.42%) aligned >1 times 97.42% overall alignment rate Time searching: 00:10:20 Overall time: 00:10:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9456544 / 47898774 = 0.1974 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:40:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:40:35: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:40:35: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:40:43: 1000000 INFO @ Wed, 08 Dec 2021 23:40:50: 2000000 INFO @ Wed, 08 Dec 2021 23:40:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:41:04: 4000000 INFO @ Wed, 08 Dec 2021 23:41:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:41:05: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:41:05: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:41:12: 5000000 INFO @ Wed, 08 Dec 2021 23:41:12: 1000000 INFO @ Wed, 08 Dec 2021 23:41:19: 2000000 INFO @ Wed, 08 Dec 2021 23:41:19: 6000000 INFO @ Wed, 08 Dec 2021 23:41:26: 3000000 INFO @ Wed, 08 Dec 2021 23:41:27: 7000000 INFO @ Wed, 08 Dec 2021 23:41:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 23:41:35: 8000000 INFO @ Wed, 08 Dec 2021 23:41:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 23:41:35: #1 read tag files... INFO @ Wed, 08 Dec 2021 23:41:35: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 23:41:40: 5000000 INFO @ Wed, 08 Dec 2021 23:41:43: 9000000 INFO @ Wed, 08 Dec 2021 23:41:43: 1000000 INFO @ Wed, 08 Dec 2021 23:41:47: 6000000 INFO @ Wed, 08 Dec 2021 23:41:51: 10000000 INFO @ Wed, 08 Dec 2021 23:41:51: 2000000 INFO @ Wed, 08 Dec 2021 23:41:55: 7000000 INFO @ Wed, 08 Dec 2021 23:41:59: 11000000 INFO @ Wed, 08 Dec 2021 23:41:59: 3000000 INFO @ Wed, 08 Dec 2021 23:42:02: 8000000 INFO @ Wed, 08 Dec 2021 23:42:07: 12000000 INFO @ Wed, 08 Dec 2021 23:42:08: 4000000 INFO @ Wed, 08 Dec 2021 23:42:09: 9000000 INFO @ Wed, 08 Dec 2021 23:42:15: 13000000 INFO @ Wed, 08 Dec 2021 23:42:16: 5000000 INFO @ Wed, 08 Dec 2021 23:42:16: 10000000 INFO @ Wed, 08 Dec 2021 23:42:23: 14000000 INFO @ Wed, 08 Dec 2021 23:42:23: 11000000 INFO @ Wed, 08 Dec 2021 23:42:24: 6000000 INFO @ Wed, 08 Dec 2021 23:42:30: 12000000 INFO @ Wed, 08 Dec 2021 23:42:31: 15000000 INFO @ Wed, 08 Dec 2021 23:42:32: 7000000 INFO @ Wed, 08 Dec 2021 23:42:38: 13000000 INFO @ Wed, 08 Dec 2021 23:42:39: 16000000 INFO @ Wed, 08 Dec 2021 23:42:40: 8000000 INFO @ Wed, 08 Dec 2021 23:42:45: 14000000 INFO @ Wed, 08 Dec 2021 23:42:47: 17000000 INFO @ Wed, 08 Dec 2021 23:42:48: 9000000 INFO @ Wed, 08 Dec 2021 23:42:52: 15000000 INFO @ Wed, 08 Dec 2021 23:42:55: 18000000 INFO @ Wed, 08 Dec 2021 23:42:56: 10000000 INFO @ Wed, 08 Dec 2021 23:42:59: 16000000 INFO @ Wed, 08 Dec 2021 23:43:03: 19000000 INFO @ Wed, 08 Dec 2021 23:43:04: 11000000 INFO @ Wed, 08 Dec 2021 23:43:06: 17000000 INFO @ Wed, 08 Dec 2021 23:43:11: 20000000 INFO @ Wed, 08 Dec 2021 23:43:12: 12000000 INFO @ Wed, 08 Dec 2021 23:43:13: 18000000 INFO @ Wed, 08 Dec 2021 23:43:19: 21000000 INFO @ Wed, 08 Dec 2021 23:43:20: 13000000 INFO @ Wed, 08 Dec 2021 23:43:21: 19000000 INFO @ Wed, 08 Dec 2021 23:43:27: 22000000 INFO @ Wed, 08 Dec 2021 23:43:28: 20000000 INFO @ Wed, 08 Dec 2021 23:43:28: 14000000 INFO @ Wed, 08 Dec 2021 23:43:35: 21000000 INFO @ Wed, 08 Dec 2021 23:43:35: 23000000 INFO @ Wed, 08 Dec 2021 23:43:36: 15000000 INFO @ Wed, 08 Dec 2021 23:43:42: 22000000 INFO @ Wed, 08 Dec 2021 23:43:43: 24000000 INFO @ Wed, 08 Dec 2021 23:43:44: 16000000 INFO @ Wed, 08 Dec 2021 23:43:49: 23000000 INFO @ Wed, 08 Dec 2021 23:43:51: 25000000 INFO @ Wed, 08 Dec 2021 23:43:52: 17000000 INFO @ Wed, 08 Dec 2021 23:43:56: 24000000 INFO @ Wed, 08 Dec 2021 23:43:59: 26000000 INFO @ Wed, 08 Dec 2021 23:44:00: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 23:44:03: 25000000 INFO @ Wed, 08 Dec 2021 23:44:07: 27000000 INFO @ Wed, 08 Dec 2021 23:44:08: 19000000 INFO @ Wed, 08 Dec 2021 23:44:10: 26000000 INFO @ Wed, 08 Dec 2021 23:44:15: 28000000 INFO @ Wed, 08 Dec 2021 23:44:16: 20000000 INFO @ Wed, 08 Dec 2021 23:44:18: 27000000 INFO @ Wed, 08 Dec 2021 23:44:22: 29000000 INFO @ Wed, 08 Dec 2021 23:44:24: 21000000 INFO @ Wed, 08 Dec 2021 23:44:25: 28000000 INFO @ Wed, 08 Dec 2021 23:44:30: 30000000 INFO @ Wed, 08 Dec 2021 23:44:32: 29000000 INFO @ Wed, 08 Dec 2021 23:44:32: 22000000 INFO @ Wed, 08 Dec 2021 23:44:38: 31000000 INFO @ Wed, 08 Dec 2021 23:44:39: 30000000 INFO @ Wed, 08 Dec 2021 23:44:40: 23000000 INFO @ Wed, 08 Dec 2021 23:44:46: 31000000 INFO @ Wed, 08 Dec 2021 23:44:46: 32000000 INFO @ Wed, 08 Dec 2021 23:44:48: 24000000 INFO @ Wed, 08 Dec 2021 23:44:53: 32000000 INFO @ Wed, 08 Dec 2021 23:44:54: 33000000 INFO @ Wed, 08 Dec 2021 23:44:56: 25000000 INFO @ Wed, 08 Dec 2021 23:45:00: 33000000 INFO @ Wed, 08 Dec 2021 23:45:02: 34000000 INFO @ Wed, 08 Dec 2021 23:45:04: 26000000 INFO @ Wed, 08 Dec 2021 23:45:07: 34000000 INFO @ Wed, 08 Dec 2021 23:45:10: 35000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 23:45:12: 27000000 INFO @ Wed, 08 Dec 2021 23:45:15: 35000000 INFO @ Wed, 08 Dec 2021 23:45:18: 36000000 INFO @ Wed, 08 Dec 2021 23:45:20: 28000000 INFO @ Wed, 08 Dec 2021 23:45:22: 36000000 INFO @ Wed, 08 Dec 2021 23:45:26: 37000000 INFO @ Wed, 08 Dec 2021 23:45:28: 29000000 INFO @ Wed, 08 Dec 2021 23:45:29: 37000000 INFO @ Wed, 08 Dec 2021 23:45:34: 38000000 INFO @ Wed, 08 Dec 2021 23:45:36: 38000000 INFO @ Wed, 08 Dec 2021 23:45:36: 30000000 INFO @ Wed, 08 Dec 2021 23:45:37: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 23:45:37: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 23:45:37: #1 total tags in treatment: 38442230 INFO @ Wed, 08 Dec 2021 23:45:37: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:45:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:45:38: #1 tags after filtering in treatment: 38442230 INFO @ Wed, 08 Dec 2021 23:45:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 23:45:38: #1 finished! INFO @ Wed, 08 Dec 2021 23:45:38: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:45:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:45:40: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 23:45:40: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 23:45:40: #1 total tags in treatment: 38442230 INFO @ Wed, 08 Dec 2021 23:45:40: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:45:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:45:40: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 23:45:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 23:45:40: Process for pairing-model is terminated! INFO @ Wed, 08 Dec 2021 23:45:40: #1 tags after filtering in treatment: 38442230 INFO @ Wed, 08 Dec 2021 23:45:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 23:45:40: #1 finished! INFO @ Wed, 08 Dec 2021 23:45:40: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:45:40: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 23:45:42: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 23:45:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 23:45:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 23:45:44: 31000000 INFO @ Wed, 08 Dec 2021 23:45:51: 32000000 INFO @ Wed, 08 Dec 2021 23:45:58: 33000000 INFO @ Wed, 08 Dec 2021 23:46:05: 34000000 INFO @ Wed, 08 Dec 2021 23:46:13: 35000000 INFO @ Wed, 08 Dec 2021 23:46:20: 36000000 INFO @ Wed, 08 Dec 2021 23:46:27: 37000000 INFO @ Wed, 08 Dec 2021 23:46:34: 38000000 INFO @ Wed, 08 Dec 2021 23:46:37: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 23:46:37: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 23:46:37: #1 total tags in treatment: 38442230 INFO @ Wed, 08 Dec 2021 23:46:37: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 23:46:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 23:46:37: #1 tags after filtering in treatment: 38442230 INFO @ Wed, 08 Dec 2021 23:46:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 23:46:37: #1 finished! INFO @ Wed, 08 Dec 2021 23:46:37: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 23:46:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 23:46:40: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 23:46:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 23:46:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3180835/SRX3180835.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling