Job ID = 6367246 SRX = SRX3104609 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:22:16 prefetch.2.10.7: 1) Downloading 'SRR5946214'... 2020-06-15T23:22:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:23:44 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:23:44 prefetch.2.10.7: 1) 'SRR5946214' was downloaded successfully 2020-06-15T23:23:44 prefetch.2.10.7: 'SRR5946214' has 0 unresolved dependencies Read 32248442 spots for SRR5946214/SRR5946214.sra Written 32248442 spots for SRR5946214/SRR5946214.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:38 32248442 reads; of these: 32248442 (100.00%) were unpaired; of these: 667757 (2.07%) aligned 0 times 26371225 (81.78%) aligned exactly 1 time 5209460 (16.15%) aligned >1 times 97.93% overall alignment rate Time searching: 00:07:39 Overall time: 00:07:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3948906 / 31580685 = 0.1250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:38:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:38:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:38:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:38:43: 1000000 INFO @ Tue, 16 Jun 2020 08:38:50: 2000000 INFO @ Tue, 16 Jun 2020 08:38:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:39:05: 4000000 INFO @ Tue, 16 Jun 2020 08:39:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:39:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:39:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:39:13: 1000000 INFO @ Tue, 16 Jun 2020 08:39:13: 5000000 INFO @ Tue, 16 Jun 2020 08:39:20: 2000000 INFO @ Tue, 16 Jun 2020 08:39:22: 6000000 INFO @ Tue, 16 Jun 2020 08:39:28: 3000000 INFO @ Tue, 16 Jun 2020 08:39:30: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:39:35: 4000000 INFO @ Tue, 16 Jun 2020 08:39:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:39:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:39:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:39:38: 8000000 INFO @ Tue, 16 Jun 2020 08:39:42: 5000000 INFO @ Tue, 16 Jun 2020 08:39:44: 1000000 INFO @ Tue, 16 Jun 2020 08:39:46: 9000000 INFO @ Tue, 16 Jun 2020 08:39:50: 6000000 INFO @ Tue, 16 Jun 2020 08:39:52: 2000000 INFO @ Tue, 16 Jun 2020 08:39:55: 10000000 INFO @ Tue, 16 Jun 2020 08:39:57: 7000000 INFO @ Tue, 16 Jun 2020 08:40:00: 3000000 INFO @ Tue, 16 Jun 2020 08:40:03: 11000000 INFO @ Tue, 16 Jun 2020 08:40:05: 8000000 INFO @ Tue, 16 Jun 2020 08:40:09: 4000000 INFO @ Tue, 16 Jun 2020 08:40:11: 12000000 INFO @ Tue, 16 Jun 2020 08:40:12: 9000000 INFO @ Tue, 16 Jun 2020 08:40:17: 5000000 INFO @ Tue, 16 Jun 2020 08:40:19: 10000000 INFO @ Tue, 16 Jun 2020 08:40:20: 13000000 INFO @ Tue, 16 Jun 2020 08:40:26: 6000000 INFO @ Tue, 16 Jun 2020 08:40:27: 11000000 INFO @ Tue, 16 Jun 2020 08:40:28: 14000000 INFO @ Tue, 16 Jun 2020 08:40:34: 7000000 INFO @ Tue, 16 Jun 2020 08:40:34: 12000000 INFO @ Tue, 16 Jun 2020 08:40:37: 15000000 INFO @ Tue, 16 Jun 2020 08:40:42: 13000000 INFO @ Tue, 16 Jun 2020 08:40:43: 8000000 INFO @ Tue, 16 Jun 2020 08:40:45: 16000000 INFO @ Tue, 16 Jun 2020 08:40:49: 14000000 INFO @ Tue, 16 Jun 2020 08:40:51: 9000000 INFO @ Tue, 16 Jun 2020 08:40:53: 17000000 INFO @ Tue, 16 Jun 2020 08:40:57: 15000000 INFO @ Tue, 16 Jun 2020 08:40:59: 10000000 INFO @ Tue, 16 Jun 2020 08:41:02: 18000000 INFO @ Tue, 16 Jun 2020 08:41:04: 16000000 INFO @ Tue, 16 Jun 2020 08:41:08: 11000000 INFO @ Tue, 16 Jun 2020 08:41:10: 19000000 INFO @ Tue, 16 Jun 2020 08:41:12: 17000000 INFO @ Tue, 16 Jun 2020 08:41:16: 12000000 INFO @ Tue, 16 Jun 2020 08:41:19: 20000000 INFO @ Tue, 16 Jun 2020 08:41:19: 18000000 INFO @ Tue, 16 Jun 2020 08:41:25: 13000000 INFO @ Tue, 16 Jun 2020 08:41:27: 19000000 INFO @ Tue, 16 Jun 2020 08:41:27: 21000000 INFO @ Tue, 16 Jun 2020 08:41:34: 14000000 INFO @ Tue, 16 Jun 2020 08:41:35: 20000000 INFO @ Tue, 16 Jun 2020 08:41:36: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:41:42: 15000000 INFO @ Tue, 16 Jun 2020 08:41:42: 21000000 INFO @ Tue, 16 Jun 2020 08:41:45: 23000000 INFO @ Tue, 16 Jun 2020 08:41:50: 22000000 INFO @ Tue, 16 Jun 2020 08:41:51: 16000000 INFO @ Tue, 16 Jun 2020 08:41:54: 24000000 INFO @ Tue, 16 Jun 2020 08:41:57: 23000000 INFO @ Tue, 16 Jun 2020 08:42:00: 17000000 INFO @ Tue, 16 Jun 2020 08:42:02: 25000000 INFO @ Tue, 16 Jun 2020 08:42:05: 24000000 INFO @ Tue, 16 Jun 2020 08:42:08: 18000000 INFO @ Tue, 16 Jun 2020 08:42:11: 26000000 INFO @ Tue, 16 Jun 2020 08:42:12: 25000000 INFO @ Tue, 16 Jun 2020 08:42:17: 19000000 INFO @ Tue, 16 Jun 2020 08:42:19: 27000000 INFO @ Tue, 16 Jun 2020 08:42:20: 26000000 INFO @ Tue, 16 Jun 2020 08:42:25: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:42:25: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:42:25: #1 total tags in treatment: 27631779 INFO @ Tue, 16 Jun 2020 08:42:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:42:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:42:25: #1 tags after filtering in treatment: 27631779 INFO @ Tue, 16 Jun 2020 08:42:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:42:25: #1 finished! INFO @ Tue, 16 Jun 2020 08:42:25: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:42:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:42:25: 20000000 INFO @ Tue, 16 Jun 2020 08:42:27: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 08:42:27: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:42:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 16 Jun 2020 08:42:27: 27000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:42:32: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:42:32: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:42:32: #1 total tags in treatment: 27631779 INFO @ Tue, 16 Jun 2020 08:42:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:42:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:42:32: #1 tags after filtering in treatment: 27631779 INFO @ Tue, 16 Jun 2020 08:42:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:42:32: #1 finished! INFO @ Tue, 16 Jun 2020 08:42:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:42:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:42:34: 21000000 INFO @ Tue, 16 Jun 2020 08:42:34: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 08:42:34: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:42:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:42:41: 22000000 INFO @ Tue, 16 Jun 2020 08:42:49: 23000000 INFO @ Tue, 16 Jun 2020 08:42:57: 24000000 INFO @ Tue, 16 Jun 2020 08:43:05: 25000000 INFO @ Tue, 16 Jun 2020 08:43:13: 26000000 INFO @ Tue, 16 Jun 2020 08:43:20: 27000000 INFO @ Tue, 16 Jun 2020 08:43:25: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:43:25: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:43:25: #1 total tags in treatment: 27631779 INFO @ Tue, 16 Jun 2020 08:43:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:43:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:43:26: #1 tags after filtering in treatment: 27631779 INFO @ Tue, 16 Jun 2020 08:43:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:43:26: #1 finished! INFO @ Tue, 16 Jun 2020 08:43:26: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:43:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:43:28: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 08:43:28: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:43:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3104609/SRX3104609.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling