Job ID = 12265584 SRX = SRX3029130 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:54:34 49621886 reads; of these: 49621886 (100.00%) were paired; of these: 18545394 (37.37%) aligned concordantly 0 times 27221544 (54.86%) aligned concordantly exactly 1 time 3854948 (7.77%) aligned concordantly >1 times ---- 18545394 pairs aligned concordantly 0 times; of these: 5325292 (28.71%) aligned discordantly 1 time ---- 13220102 pairs aligned 0 times concordantly or discordantly; of these: 26440204 mates make up the pairs; of these: 23938571 (90.54%) aligned 0 times 1145624 (4.33%) aligned exactly 1 time 1356009 (5.13%) aligned >1 times 75.88% overall alignment rate Time searching: 00:54:34 Overall time: 00:54:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8196015 / 35988908 = 0.2277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:12:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:12:29: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:12:29: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:12:37: 1000000 INFO @ Sat, 03 Apr 2021 09:12:46: 2000000 INFO @ Sat, 03 Apr 2021 09:12:52: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:12:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:12:58: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:12:58: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:13:00: 4000000 INFO @ Sat, 03 Apr 2021 09:13:07: 1000000 INFO @ Sat, 03 Apr 2021 09:13:08: 5000000 INFO @ Sat, 03 Apr 2021 09:13:15: 2000000 INFO @ Sat, 03 Apr 2021 09:13:17: 6000000 INFO @ Sat, 03 Apr 2021 09:13:23: 3000000 INFO @ Sat, 03 Apr 2021 09:13:24: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:13:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:13:28: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:13:28: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:13:31: 4000000 INFO @ Sat, 03 Apr 2021 09:13:33: 8000000 INFO @ Sat, 03 Apr 2021 09:13:38: 1000000 INFO @ Sat, 03 Apr 2021 09:13:40: 5000000 INFO @ Sat, 03 Apr 2021 09:13:42: 9000000 INFO @ Sat, 03 Apr 2021 09:13:48: 2000000 INFO @ Sat, 03 Apr 2021 09:13:49: 6000000 INFO @ Sat, 03 Apr 2021 09:13:50: 10000000 INFO @ Sat, 03 Apr 2021 09:13:56: 3000000 INFO @ Sat, 03 Apr 2021 09:13:58: 7000000 INFO @ Sat, 03 Apr 2021 09:13:59: 11000000 INFO @ Sat, 03 Apr 2021 09:14:04: 4000000 INFO @ Sat, 03 Apr 2021 09:14:07: 8000000 INFO @ Sat, 03 Apr 2021 09:14:09: 12000000 INFO @ Sat, 03 Apr 2021 09:14:12: 5000000 INFO @ Sat, 03 Apr 2021 09:14:17: 9000000 INFO @ Sat, 03 Apr 2021 09:14:20: 13000000 INFO @ Sat, 03 Apr 2021 09:14:20: 6000000 INFO @ Sat, 03 Apr 2021 09:14:28: 10000000 INFO @ Sat, 03 Apr 2021 09:14:28: 7000000 INFO @ Sat, 03 Apr 2021 09:14:30: 14000000 INFO @ Sat, 03 Apr 2021 09:14:36: 8000000 INFO @ Sat, 03 Apr 2021 09:14:37: 11000000 INFO @ Sat, 03 Apr 2021 09:14:39: 15000000 INFO @ Sat, 03 Apr 2021 09:14:44: 9000000 INFO @ Sat, 03 Apr 2021 09:14:45: 12000000 INFO @ Sat, 03 Apr 2021 09:14:47: 16000000 INFO @ Sat, 03 Apr 2021 09:14:51: 10000000 INFO @ Sat, 03 Apr 2021 09:14:54: 13000000 INFO @ Sat, 03 Apr 2021 09:14:56: 17000000 INFO @ Sat, 03 Apr 2021 09:15:00: 11000000 INFO @ Sat, 03 Apr 2021 09:15:03: 14000000 INFO @ Sat, 03 Apr 2021 09:15:05: 18000000 INFO @ Sat, 03 Apr 2021 09:15:08: 12000000 INFO @ Sat, 03 Apr 2021 09:15:13: 15000000 INFO @ Sat, 03 Apr 2021 09:15:15: 19000000 INFO @ Sat, 03 Apr 2021 09:15:16: 13000000 INFO @ Sat, 03 Apr 2021 09:15:21: 16000000 INFO @ Sat, 03 Apr 2021 09:15:24: 20000000 INFO @ Sat, 03 Apr 2021 09:15:25: 14000000 INFO @ Sat, 03 Apr 2021 09:15:30: 17000000 INFO @ Sat, 03 Apr 2021 09:15:33: 21000000 INFO @ Sat, 03 Apr 2021 09:15:33: 15000000 INFO @ Sat, 03 Apr 2021 09:15:39: 18000000 INFO @ Sat, 03 Apr 2021 09:15:41: 16000000 INFO @ Sat, 03 Apr 2021 09:15:43: 22000000 INFO @ Sat, 03 Apr 2021 09:15:48: 19000000 INFO @ Sat, 03 Apr 2021 09:15:49: 17000000 INFO @ Sat, 03 Apr 2021 09:15:51: 23000000 INFO @ Sat, 03 Apr 2021 09:15:57: 18000000 INFO @ Sat, 03 Apr 2021 09:15:58: 20000000 INFO @ Sat, 03 Apr 2021 09:16:02: 24000000 INFO @ Sat, 03 Apr 2021 09:16:05: 19000000 INFO @ Sat, 03 Apr 2021 09:16:07: 21000000 INFO @ Sat, 03 Apr 2021 09:16:11: 25000000 INFO @ Sat, 03 Apr 2021 09:16:13: 20000000 INFO @ Sat, 03 Apr 2021 09:16:16: 22000000 INFO @ Sat, 03 Apr 2021 09:16:19: 26000000 INFO @ Sat, 03 Apr 2021 09:16:21: 21000000 INFO @ Sat, 03 Apr 2021 09:16:25: 23000000 INFO @ Sat, 03 Apr 2021 09:16:28: 27000000 INFO @ Sat, 03 Apr 2021 09:16:30: 22000000 INFO @ Sat, 03 Apr 2021 09:16:34: 24000000 INFO @ Sat, 03 Apr 2021 09:16:37: 28000000 INFO @ Sat, 03 Apr 2021 09:16:38: 23000000 INFO @ Sat, 03 Apr 2021 09:16:43: 25000000 INFO @ Sat, 03 Apr 2021 09:16:46: 29000000 INFO @ Sat, 03 Apr 2021 09:16:46: 24000000 INFO @ Sat, 03 Apr 2021 09:16:51: 26000000 INFO @ Sat, 03 Apr 2021 09:16:55: 25000000 INFO @ Sat, 03 Apr 2021 09:16:55: 30000000 INFO @ Sat, 03 Apr 2021 09:17:00: 27000000 INFO @ Sat, 03 Apr 2021 09:17:03: 26000000 INFO @ Sat, 03 Apr 2021 09:17:04: 31000000 INFO @ Sat, 03 Apr 2021 09:17:09: 28000000 INFO @ Sat, 03 Apr 2021 09:17:11: 27000000 INFO @ Sat, 03 Apr 2021 09:17:13: 32000000 INFO @ Sat, 03 Apr 2021 09:17:18: 29000000 INFO @ Sat, 03 Apr 2021 09:17:19: 28000000 INFO @ Sat, 03 Apr 2021 09:17:22: 33000000 INFO @ Sat, 03 Apr 2021 09:17:26: 30000000 INFO @ Sat, 03 Apr 2021 09:17:27: 29000000 INFO @ Sat, 03 Apr 2021 09:17:30: 34000000 INFO @ Sat, 03 Apr 2021 09:17:35: 30000000 INFO @ Sat, 03 Apr 2021 09:17:36: 31000000 INFO @ Sat, 03 Apr 2021 09:17:40: 35000000 INFO @ Sat, 03 Apr 2021 09:17:43: 31000000 INFO @ Sat, 03 Apr 2021 09:17:45: 32000000 INFO @ Sat, 03 Apr 2021 09:17:49: 36000000 INFO @ Sat, 03 Apr 2021 09:17:51: 32000000 INFO @ Sat, 03 Apr 2021 09:17:53: 33000000 INFO @ Sat, 03 Apr 2021 09:17:57: 37000000 INFO @ Sat, 03 Apr 2021 09:17:59: 33000000 INFO @ Sat, 03 Apr 2021 09:18:03: 34000000 INFO @ Sat, 03 Apr 2021 09:18:07: 34000000 INFO @ Sat, 03 Apr 2021 09:18:07: 38000000 INFO @ Sat, 03 Apr 2021 09:18:13: 35000000 INFO @ Sat, 03 Apr 2021 09:18:15: 35000000 INFO @ Sat, 03 Apr 2021 09:18:18: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:18:23: 36000000 INFO @ Sat, 03 Apr 2021 09:18:24: 36000000 INFO @ Sat, 03 Apr 2021 09:18:28: 40000000 INFO @ Sat, 03 Apr 2021 09:18:30: 37000000 INFO @ Sat, 03 Apr 2021 09:18:34: 37000000 INFO @ Sat, 03 Apr 2021 09:18:38: 38000000 INFO @ Sat, 03 Apr 2021 09:18:39: 41000000 INFO @ Sat, 03 Apr 2021 09:18:45: 38000000 INFO @ Sat, 03 Apr 2021 09:18:46: 39000000 INFO @ Sat, 03 Apr 2021 09:18:50: 42000000 INFO @ Sat, 03 Apr 2021 09:18:54: 40000000 INFO @ Sat, 03 Apr 2021 09:18:55: 39000000 INFO @ Sat, 03 Apr 2021 09:19:00: 43000000 INFO @ Sat, 03 Apr 2021 09:19:02: 41000000 INFO @ Sat, 03 Apr 2021 09:19:05: 40000000 INFO @ Sat, 03 Apr 2021 09:19:10: 42000000 INFO @ Sat, 03 Apr 2021 09:19:12: 44000000 INFO @ Sat, 03 Apr 2021 09:19:17: 41000000 INFO @ Sat, 03 Apr 2021 09:19:19: 43000000 INFO @ Sat, 03 Apr 2021 09:19:24: 45000000 INFO @ Sat, 03 Apr 2021 09:19:28: 44000000 INFO @ Sat, 03 Apr 2021 09:19:29: 42000000 INFO @ Sat, 03 Apr 2021 09:19:35: 46000000 INFO @ Sat, 03 Apr 2021 09:19:36: 45000000 INFO @ Sat, 03 Apr 2021 09:19:40: 43000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:19:45: 46000000 INFO @ Sat, 03 Apr 2021 09:19:47: 47000000 INFO @ Sat, 03 Apr 2021 09:19:52: 44000000 INFO @ Sat, 03 Apr 2021 09:19:55: 47000000 INFO @ Sat, 03 Apr 2021 09:19:59: 48000000 INFO @ Sat, 03 Apr 2021 09:20:03: 45000000 INFO @ Sat, 03 Apr 2021 09:20:04: 48000000 INFO @ Sat, 03 Apr 2021 09:20:10: 49000000 INFO @ Sat, 03 Apr 2021 09:20:14: 49000000 INFO @ Sat, 03 Apr 2021 09:20:14: 46000000 INFO @ Sat, 03 Apr 2021 09:20:22: 50000000 INFO @ Sat, 03 Apr 2021 09:20:24: 50000000 INFO @ Sat, 03 Apr 2021 09:20:26: 47000000 INFO @ Sat, 03 Apr 2021 09:20:33: 51000000 INFO @ Sat, 03 Apr 2021 09:20:35: 51000000 INFO @ Sat, 03 Apr 2021 09:20:39: 48000000 INFO @ Sat, 03 Apr 2021 09:20:42: 52000000 INFO @ Sat, 03 Apr 2021 09:20:47: 52000000 INFO @ Sat, 03 Apr 2021 09:20:50: 53000000 INFO @ Sat, 03 Apr 2021 09:20:51: 49000000 INFO @ Sat, 03 Apr 2021 09:20:59: 54000000 INFO @ Sat, 03 Apr 2021 09:20:59: 53000000 INFO @ Sat, 03 Apr 2021 09:21:03: 50000000 INFO @ Sat, 03 Apr 2021 09:21:08: 55000000 INFO @ Sat, 03 Apr 2021 09:21:11: 54000000 INFO @ Sat, 03 Apr 2021 09:21:15: 51000000 INFO @ Sat, 03 Apr 2021 09:21:17: 56000000 INFO @ Sat, 03 Apr 2021 09:21:23: 55000000 INFO @ Sat, 03 Apr 2021 09:21:25: 57000000 INFO @ Sat, 03 Apr 2021 09:21:26: 52000000 INFO @ Sat, 03 Apr 2021 09:21:34: 56000000 INFO @ Sat, 03 Apr 2021 09:21:34: 58000000 INFO @ Sat, 03 Apr 2021 09:21:37: 53000000 INFO @ Sat, 03 Apr 2021 09:21:42: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:21:42: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:21:42: #1 total tags in treatment: 24214533 INFO @ Sat, 03 Apr 2021 09:21:42: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:21:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:21:42: #1 tags after filtering in treatment: 16701708 INFO @ Sat, 03 Apr 2021 09:21:42: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 09:21:42: #1 finished! INFO @ Sat, 03 Apr 2021 09:21:42: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:21:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:21:43: #2 number of paired peaks: 162 WARNING @ Sat, 03 Apr 2021 09:21:43: Fewer paired peaks (162) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 162 pairs to build model! INFO @ Sat, 03 Apr 2021 09:21:43: start model_add_line... INFO @ Sat, 03 Apr 2021 09:21:44: start X-correlation... INFO @ Sat, 03 Apr 2021 09:21:44: end of X-cor INFO @ Sat, 03 Apr 2021 09:21:44: #2 finished! INFO @ Sat, 03 Apr 2021 09:21:44: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 09:21:44: #2 alternative fragment length(s) may be 4,100,597 bps INFO @ Sat, 03 Apr 2021 09:21:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.20_model.r WARNING @ Sat, 03 Apr 2021 09:21:44: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:21:44: #2 You may need to consider one of the other alternative d(s): 4,100,597 WARNING @ Sat, 03 Apr 2021 09:21:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:21:44: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:21:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:21:44: 57000000 INFO @ Sat, 03 Apr 2021 09:21:47: 54000000 INFO @ Sat, 03 Apr 2021 09:21:54: 58000000 INFO @ Sat, 03 Apr 2021 09:21:58: 55000000 INFO @ Sat, 03 Apr 2021 09:22:05: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:22:05: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:22:05: #1 total tags in treatment: 24214533 INFO @ Sat, 03 Apr 2021 09:22:05: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:22:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:22:05: #1 tags after filtering in treatment: 16701708 INFO @ Sat, 03 Apr 2021 09:22:05: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 09:22:05: #1 finished! INFO @ Sat, 03 Apr 2021 09:22:05: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:22:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:22:06: #2 number of paired peaks: 162 WARNING @ Sat, 03 Apr 2021 09:22:06: Fewer paired peaks (162) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 162 pairs to build model! INFO @ Sat, 03 Apr 2021 09:22:06: start model_add_line... INFO @ Sat, 03 Apr 2021 09:22:06: start X-correlation... INFO @ Sat, 03 Apr 2021 09:22:06: end of X-cor INFO @ Sat, 03 Apr 2021 09:22:06: #2 finished! INFO @ Sat, 03 Apr 2021 09:22:06: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 09:22:06: #2 alternative fragment length(s) may be 4,100,597 bps INFO @ Sat, 03 Apr 2021 09:22:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.05_model.r WARNING @ Sat, 03 Apr 2021 09:22:06: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:22:06: #2 You may need to consider one of the other alternative d(s): 4,100,597 WARNING @ Sat, 03 Apr 2021 09:22:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:22:06: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:22:06: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:22:09: 56000000 INFO @ Sat, 03 Apr 2021 09:22:15: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:22:20: 57000000 INFO @ Sat, 03 Apr 2021 09:22:31: 58000000 INFO @ Sat, 03 Apr 2021 09:22:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:22:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:22:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.20_summits.bed INFO @ Sat, 03 Apr 2021 09:22:32: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1762 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:22:38: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:22:41: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:22:41: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:22:41: #1 total tags in treatment: 24214533 INFO @ Sat, 03 Apr 2021 09:22:41: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:22:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:22:41: #1 tags after filtering in treatment: 16701708 INFO @ Sat, 03 Apr 2021 09:22:41: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 09:22:41: #1 finished! INFO @ Sat, 03 Apr 2021 09:22:41: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:22:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:22:43: #2 number of paired peaks: 162 WARNING @ Sat, 03 Apr 2021 09:22:43: Fewer paired peaks (162) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 162 pairs to build model! INFO @ Sat, 03 Apr 2021 09:22:43: start model_add_line... INFO @ Sat, 03 Apr 2021 09:22:43: start X-correlation... INFO @ Sat, 03 Apr 2021 09:22:43: end of X-cor INFO @ Sat, 03 Apr 2021 09:22:43: #2 finished! INFO @ Sat, 03 Apr 2021 09:22:43: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 09:22:43: #2 alternative fragment length(s) may be 4,100,597 bps INFO @ Sat, 03 Apr 2021 09:22:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.10_model.r WARNING @ Sat, 03 Apr 2021 09:22:43: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:22:43: #2 You may need to consider one of the other alternative d(s): 4,100,597 WARNING @ Sat, 03 Apr 2021 09:22:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:22:43: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:22:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:22:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:22:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:22:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.05_summits.bed INFO @ Sat, 03 Apr 2021 09:22:57: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (8467 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:23:14: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:23:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:23:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:23:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029130/SRX3029130.10_summits.bed INFO @ Sat, 03 Apr 2021 09:23:32: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (4662 records, 4 fields): 7 millis CompletedMACS2peakCalling