Job ID = 12265624 SRX = SRX3029125 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:56:13 54995702 reads; of these: 54995702 (100.00%) were paired; of these: 25094808 (45.63%) aligned concordantly 0 times 26027399 (47.33%) aligned concordantly exactly 1 time 3873495 (7.04%) aligned concordantly >1 times ---- 25094808 pairs aligned concordantly 0 times; of these: 6798180 (27.09%) aligned discordantly 1 time ---- 18296628 pairs aligned 0 times concordantly or discordantly; of these: 36593256 mates make up the pairs; of these: 33418219 (91.32%) aligned 0 times 1512137 (4.13%) aligned exactly 1 time 1662900 (4.54%) aligned >1 times 69.62% overall alignment rate Time searching: 00:56:13 Overall time: 00:56:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8326533 / 36227183 = 0.2298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:27:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:27:02: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:27:02: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:27:10: 1000000 INFO @ Sat, 03 Apr 2021 09:27:17: 2000000 INFO @ Sat, 03 Apr 2021 09:27:25: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:27:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:27:32: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:27:32: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:27:32: 4000000 INFO @ Sat, 03 Apr 2021 09:27:40: 1000000 INFO @ Sat, 03 Apr 2021 09:27:40: 5000000 INFO @ Sat, 03 Apr 2021 09:27:48: 2000000 INFO @ Sat, 03 Apr 2021 09:27:48: 6000000 INFO @ Sat, 03 Apr 2021 09:27:56: 7000000 INFO @ Sat, 03 Apr 2021 09:27:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:28:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:28:02: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:28:02: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:28:04: 8000000 INFO @ Sat, 03 Apr 2021 09:28:04: 4000000 INFO @ Sat, 03 Apr 2021 09:28:11: 1000000 INFO @ Sat, 03 Apr 2021 09:28:12: 5000000 INFO @ Sat, 03 Apr 2021 09:28:13: 9000000 INFO @ Sat, 03 Apr 2021 09:28:20: 2000000 INFO @ Sat, 03 Apr 2021 09:28:21: 6000000 INFO @ Sat, 03 Apr 2021 09:28:22: 10000000 INFO @ Sat, 03 Apr 2021 09:28:28: 3000000 INFO @ Sat, 03 Apr 2021 09:28:30: 7000000 INFO @ Sat, 03 Apr 2021 09:28:31: 11000000 INFO @ Sat, 03 Apr 2021 09:28:36: 4000000 INFO @ Sat, 03 Apr 2021 09:28:39: 8000000 INFO @ Sat, 03 Apr 2021 09:28:40: 12000000 INFO @ Sat, 03 Apr 2021 09:28:44: 5000000 INFO @ Sat, 03 Apr 2021 09:28:48: 9000000 INFO @ Sat, 03 Apr 2021 09:28:49: 13000000 INFO @ Sat, 03 Apr 2021 09:28:52: 6000000 INFO @ Sat, 03 Apr 2021 09:28:57: 10000000 INFO @ Sat, 03 Apr 2021 09:28:58: 14000000 INFO @ Sat, 03 Apr 2021 09:29:00: 7000000 INFO @ Sat, 03 Apr 2021 09:29:06: 11000000 INFO @ Sat, 03 Apr 2021 09:29:08: 15000000 INFO @ Sat, 03 Apr 2021 09:29:08: 8000000 INFO @ Sat, 03 Apr 2021 09:29:15: 12000000 INFO @ Sat, 03 Apr 2021 09:29:17: 9000000 INFO @ Sat, 03 Apr 2021 09:29:17: 16000000 INFO @ Sat, 03 Apr 2021 09:29:24: 10000000 INFO @ Sat, 03 Apr 2021 09:29:25: 13000000 INFO @ Sat, 03 Apr 2021 09:29:26: 17000000 INFO @ Sat, 03 Apr 2021 09:29:32: 11000000 INFO @ Sat, 03 Apr 2021 09:29:34: 14000000 INFO @ Sat, 03 Apr 2021 09:29:35: 18000000 INFO @ Sat, 03 Apr 2021 09:29:40: 12000000 INFO @ Sat, 03 Apr 2021 09:29:43: 15000000 INFO @ Sat, 03 Apr 2021 09:29:45: 19000000 INFO @ Sat, 03 Apr 2021 09:29:48: 13000000 INFO @ Sat, 03 Apr 2021 09:29:52: 16000000 INFO @ Sat, 03 Apr 2021 09:29:53: 20000000 INFO @ Sat, 03 Apr 2021 09:29:57: 14000000 INFO @ Sat, 03 Apr 2021 09:30:01: 17000000 INFO @ Sat, 03 Apr 2021 09:30:02: 21000000 INFO @ Sat, 03 Apr 2021 09:30:05: 15000000 INFO @ Sat, 03 Apr 2021 09:30:10: 18000000 INFO @ Sat, 03 Apr 2021 09:30:10: 22000000 INFO @ Sat, 03 Apr 2021 09:30:13: 16000000 INFO @ Sat, 03 Apr 2021 09:30:19: 19000000 INFO @ Sat, 03 Apr 2021 09:30:20: 23000000 INFO @ Sat, 03 Apr 2021 09:30:23: 17000000 INFO @ Sat, 03 Apr 2021 09:30:29: 20000000 INFO @ Sat, 03 Apr 2021 09:30:29: 24000000 INFO @ Sat, 03 Apr 2021 09:30:32: 18000000 INFO @ Sat, 03 Apr 2021 09:30:38: 21000000 INFO @ Sat, 03 Apr 2021 09:30:38: 25000000 INFO @ Sat, 03 Apr 2021 09:30:40: 19000000 INFO @ Sat, 03 Apr 2021 09:30:47: 22000000 INFO @ Sat, 03 Apr 2021 09:30:47: 26000000 INFO @ Sat, 03 Apr 2021 09:30:48: 20000000 INFO @ Sat, 03 Apr 2021 09:30:55: 27000000 INFO @ Sat, 03 Apr 2021 09:30:56: 21000000 INFO @ Sat, 03 Apr 2021 09:30:56: 23000000 INFO @ Sat, 03 Apr 2021 09:31:03: 22000000 INFO @ Sat, 03 Apr 2021 09:31:04: 28000000 INFO @ Sat, 03 Apr 2021 09:31:04: 24000000 INFO @ Sat, 03 Apr 2021 09:31:11: 23000000 INFO @ Sat, 03 Apr 2021 09:31:13: 25000000 INFO @ Sat, 03 Apr 2021 09:31:14: 29000000 INFO @ Sat, 03 Apr 2021 09:31:19: 24000000 INFO @ Sat, 03 Apr 2021 09:31:22: 26000000 INFO @ Sat, 03 Apr 2021 09:31:22: 30000000 INFO @ Sat, 03 Apr 2021 09:31:27: 25000000 INFO @ Sat, 03 Apr 2021 09:31:31: 27000000 INFO @ Sat, 03 Apr 2021 09:31:31: 31000000 INFO @ Sat, 03 Apr 2021 09:31:35: 26000000 INFO @ Sat, 03 Apr 2021 09:31:40: 28000000 INFO @ Sat, 03 Apr 2021 09:31:40: 32000000 INFO @ Sat, 03 Apr 2021 09:31:43: 27000000 INFO @ Sat, 03 Apr 2021 09:31:48: 33000000 INFO @ Sat, 03 Apr 2021 09:31:49: 29000000 INFO @ Sat, 03 Apr 2021 09:31:51: 28000000 INFO @ Sat, 03 Apr 2021 09:31:57: 34000000 INFO @ Sat, 03 Apr 2021 09:31:58: 30000000 INFO @ Sat, 03 Apr 2021 09:31:59: 29000000 INFO @ Sat, 03 Apr 2021 09:32:06: 35000000 INFO @ Sat, 03 Apr 2021 09:32:06: 31000000 INFO @ Sat, 03 Apr 2021 09:32:07: 30000000 INFO @ Sat, 03 Apr 2021 09:32:14: 31000000 INFO @ Sat, 03 Apr 2021 09:32:15: 36000000 INFO @ Sat, 03 Apr 2021 09:32:15: 32000000 INFO @ Sat, 03 Apr 2021 09:32:22: 32000000 INFO @ Sat, 03 Apr 2021 09:32:23: 37000000 INFO @ Sat, 03 Apr 2021 09:32:24: 33000000 INFO @ Sat, 03 Apr 2021 09:32:30: 33000000 INFO @ Sat, 03 Apr 2021 09:32:32: 38000000 INFO @ Sat, 03 Apr 2021 09:32:33: 34000000 INFO @ Sat, 03 Apr 2021 09:32:37: 34000000 INFO @ Sat, 03 Apr 2021 09:32:41: 39000000 INFO @ Sat, 03 Apr 2021 09:32:42: 35000000 INFO @ Sat, 03 Apr 2021 09:32:45: 35000000 INFO @ Sat, 03 Apr 2021 09:32:50: 40000000 INFO @ Sat, 03 Apr 2021 09:32:50: 36000000 INFO @ Sat, 03 Apr 2021 09:32:53: 36000000 INFO @ Sat, 03 Apr 2021 09:32:59: 41000000 INFO @ Sat, 03 Apr 2021 09:32:59: 37000000 INFO @ Sat, 03 Apr 2021 09:33:00: 37000000 INFO @ Sat, 03 Apr 2021 09:33:07: 42000000 INFO @ Sat, 03 Apr 2021 09:33:08: 38000000 INFO @ Sat, 03 Apr 2021 09:33:08: 38000000 INFO @ Sat, 03 Apr 2021 09:33:16: 39000000 INFO @ Sat, 03 Apr 2021 09:33:16: 43000000 INFO @ Sat, 03 Apr 2021 09:33:17: 39000000 INFO @ Sat, 03 Apr 2021 09:33:24: 40000000 INFO @ Sat, 03 Apr 2021 09:33:25: 44000000 INFO @ Sat, 03 Apr 2021 09:33:25: 40000000 INFO @ Sat, 03 Apr 2021 09:33:32: 41000000 INFO @ Sat, 03 Apr 2021 09:33:34: 45000000 INFO @ Sat, 03 Apr 2021 09:33:34: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:33:40: 42000000 INFO @ Sat, 03 Apr 2021 09:33:43: 42000000 INFO @ Sat, 03 Apr 2021 09:33:44: 46000000 INFO @ Sat, 03 Apr 2021 09:33:49: 43000000 INFO @ Sat, 03 Apr 2021 09:33:51: 43000000 INFO @ Sat, 03 Apr 2021 09:33:53: 47000000 INFO @ Sat, 03 Apr 2021 09:33:58: 44000000 INFO @ Sat, 03 Apr 2021 09:34:00: 44000000 INFO @ Sat, 03 Apr 2021 09:34:03: 48000000 INFO @ Sat, 03 Apr 2021 09:34:07: 45000000 INFO @ Sat, 03 Apr 2021 09:34:09: 45000000 INFO @ Sat, 03 Apr 2021 09:34:12: 49000000 INFO @ Sat, 03 Apr 2021 09:34:17: 46000000 INFO @ Sat, 03 Apr 2021 09:34:19: 46000000 INFO @ Sat, 03 Apr 2021 09:34:22: 50000000 INFO @ Sat, 03 Apr 2021 09:34:27: 47000000 INFO @ Sat, 03 Apr 2021 09:34:29: 47000000 INFO @ Sat, 03 Apr 2021 09:34:32: 51000000 INFO @ Sat, 03 Apr 2021 09:34:36: 48000000 INFO @ Sat, 03 Apr 2021 09:34:39: 48000000 INFO @ Sat, 03 Apr 2021 09:34:41: 52000000 INFO @ Sat, 03 Apr 2021 09:34:46: 49000000 INFO @ Sat, 03 Apr 2021 09:34:49: 49000000 INFO @ Sat, 03 Apr 2021 09:34:51: 53000000 INFO @ Sat, 03 Apr 2021 09:34:55: 50000000 INFO @ Sat, 03 Apr 2021 09:34:58: 50000000 INFO @ Sat, 03 Apr 2021 09:35:00: 54000000 INFO @ Sat, 03 Apr 2021 09:35:03: 51000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:35:08: 51000000 INFO @ Sat, 03 Apr 2021 09:35:10: 55000000 INFO @ Sat, 03 Apr 2021 09:35:12: 52000000 INFO @ Sat, 03 Apr 2021 09:35:17: 52000000 INFO @ Sat, 03 Apr 2021 09:35:19: 56000000 INFO @ Sat, 03 Apr 2021 09:35:20: 53000000 INFO @ Sat, 03 Apr 2021 09:35:27: 53000000 INFO @ Sat, 03 Apr 2021 09:35:28: 54000000 INFO @ Sat, 03 Apr 2021 09:35:29: 57000000 INFO @ Sat, 03 Apr 2021 09:35:36: 54000000 INFO @ Sat, 03 Apr 2021 09:35:37: 55000000 INFO @ Sat, 03 Apr 2021 09:35:39: 58000000 INFO @ Sat, 03 Apr 2021 09:35:45: 56000000 INFO @ Sat, 03 Apr 2021 09:35:46: 55000000 INFO @ Sat, 03 Apr 2021 09:35:48: 59000000 INFO @ Sat, 03 Apr 2021 09:35:54: 57000000 INFO @ Sat, 03 Apr 2021 09:35:55: 56000000 INFO @ Sat, 03 Apr 2021 09:35:56: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:35:56: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:35:56: #1 total tags in treatment: 23341771 INFO @ Sat, 03 Apr 2021 09:35:56: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:35:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:35:56: #1 tags after filtering in treatment: 16014829 INFO @ Sat, 03 Apr 2021 09:35:56: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 09:35:56: #1 finished! INFO @ Sat, 03 Apr 2021 09:35:56: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:35:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:35:58: #2 number of paired peaks: 202 WARNING @ Sat, 03 Apr 2021 09:35:58: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Sat, 03 Apr 2021 09:35:58: start model_add_line... INFO @ Sat, 03 Apr 2021 09:35:58: start X-correlation... INFO @ Sat, 03 Apr 2021 09:35:58: end of X-cor INFO @ Sat, 03 Apr 2021 09:35:58: #2 finished! INFO @ Sat, 03 Apr 2021 09:35:58: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 09:35:58: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 09:35:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.05_model.r WARNING @ Sat, 03 Apr 2021 09:35:58: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:35:58: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 09:35:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:35:58: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:35:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:36:02: 58000000 INFO @ Sat, 03 Apr 2021 09:36:03: 57000000 INFO @ Sat, 03 Apr 2021 09:36:10: 59000000 INFO @ Sat, 03 Apr 2021 09:36:11: 58000000 INFO @ Sat, 03 Apr 2021 09:36:18: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:36:18: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:36:18: #1 total tags in treatment: 23341771 INFO @ Sat, 03 Apr 2021 09:36:18: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:36:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:36:18: #1 tags after filtering in treatment: 16014829 INFO @ Sat, 03 Apr 2021 09:36:18: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 09:36:18: #1 finished! INFO @ Sat, 03 Apr 2021 09:36:18: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:36:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:36:19: 59000000 INFO @ Sat, 03 Apr 2021 09:36:20: #2 number of paired peaks: 202 WARNING @ Sat, 03 Apr 2021 09:36:20: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Sat, 03 Apr 2021 09:36:20: start model_add_line... INFO @ Sat, 03 Apr 2021 09:36:20: start X-correlation... INFO @ Sat, 03 Apr 2021 09:36:20: end of X-cor INFO @ Sat, 03 Apr 2021 09:36:20: #2 finished! INFO @ Sat, 03 Apr 2021 09:36:20: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 09:36:20: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 09:36:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.20_model.r WARNING @ Sat, 03 Apr 2021 09:36:20: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:36:20: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 09:36:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:36:20: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:36:20: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:36:26: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:36:26: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:36:26: #1 total tags in treatment: 23341771 INFO @ Sat, 03 Apr 2021 09:36:26: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:36:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:36:27: #1 tags after filtering in treatment: 16014829 INFO @ Sat, 03 Apr 2021 09:36:27: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 09:36:27: #1 finished! INFO @ Sat, 03 Apr 2021 09:36:27: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:36:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:36:28: #2 number of paired peaks: 202 WARNING @ Sat, 03 Apr 2021 09:36:28: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Sat, 03 Apr 2021 09:36:28: start model_add_line... INFO @ Sat, 03 Apr 2021 09:36:28: start X-correlation... INFO @ Sat, 03 Apr 2021 09:36:28: end of X-cor INFO @ Sat, 03 Apr 2021 09:36:28: #2 finished! INFO @ Sat, 03 Apr 2021 09:36:28: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 09:36:28: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 09:36:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.10_model.r WARNING @ Sat, 03 Apr 2021 09:36:28: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:36:28: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 09:36:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:36:28: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:36:28: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:36:40: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:37:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:37:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:37:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.05_summits.bed INFO @ Sat, 03 Apr 2021 09:37:01: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10092 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:37:05: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:37:10: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:37:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.20_peaks.xls INFO @ Sat, 03 Apr 2021 09:37:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:37:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.20_summits.bed INFO @ Sat, 03 Apr 2021 09:37:27: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (2349 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:37:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:37:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:37:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029125/SRX3029125.10_summits.bed INFO @ Sat, 03 Apr 2021 09:37:31: Done! pass1 - making usageList (7 chroms): 4 millis pass2 - checking and writing primary data (5922 records, 4 fields): 11 millis CompletedMACS2peakCalling