Job ID = 12265653 SRX = SRX3029124 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:06:19 61195528 reads; of these: 61195528 (100.00%) were paired; of these: 24558368 (40.13%) aligned concordantly 0 times 31829272 (52.01%) aligned concordantly exactly 1 time 4807888 (7.86%) aligned concordantly >1 times ---- 24558368 pairs aligned concordantly 0 times; of these: 7662877 (31.20%) aligned discordantly 1 time ---- 16895491 pairs aligned 0 times concordantly or discordantly; of these: 33790982 mates make up the pairs; of these: 30224742 (89.45%) aligned 0 times 1621007 (4.80%) aligned exactly 1 time 1945233 (5.76%) aligned >1 times 75.30% overall alignment rate Time searching: 01:06:19 Overall time: 01:06:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 11717949 / 43733165 = 0.2679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:48:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:48:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:49:01: 1000000 INFO @ Sat, 03 Apr 2021 09:49:08: 2000000 INFO @ Sat, 03 Apr 2021 09:49:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:49:23: 4000000 INFO @ Sat, 03 Apr 2021 09:49:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:49:24: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:49:24: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:49:30: 5000000 INFO @ Sat, 03 Apr 2021 09:49:32: 1000000 INFO @ Sat, 03 Apr 2021 09:49:38: 6000000 INFO @ Sat, 03 Apr 2021 09:49:40: 2000000 INFO @ Sat, 03 Apr 2021 09:49:46: 7000000 INFO @ Sat, 03 Apr 2021 09:49:48: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 09:49:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 09:49:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 09:49:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 09:49:54: 8000000 INFO @ Sat, 03 Apr 2021 09:49:56: 4000000 INFO @ Sat, 03 Apr 2021 09:50:01: 1000000 INFO @ Sat, 03 Apr 2021 09:50:03: 9000000 INFO @ Sat, 03 Apr 2021 09:50:05: 5000000 INFO @ Sat, 03 Apr 2021 09:50:09: 2000000 INFO @ Sat, 03 Apr 2021 09:50:11: 10000000 INFO @ Sat, 03 Apr 2021 09:50:13: 6000000 INFO @ Sat, 03 Apr 2021 09:50:16: 3000000 INFO @ Sat, 03 Apr 2021 09:50:19: 11000000 INFO @ Sat, 03 Apr 2021 09:50:21: 7000000 INFO @ Sat, 03 Apr 2021 09:50:22: 4000000 INFO @ Sat, 03 Apr 2021 09:50:27: 12000000 INFO @ Sat, 03 Apr 2021 09:50:29: 8000000 INFO @ Sat, 03 Apr 2021 09:50:32: 5000000 INFO @ Sat, 03 Apr 2021 09:50:35: 13000000 INFO @ Sat, 03 Apr 2021 09:50:37: 9000000 INFO @ Sat, 03 Apr 2021 09:50:40: 6000000 INFO @ Sat, 03 Apr 2021 09:50:43: 14000000 INFO @ Sat, 03 Apr 2021 09:50:44: 10000000 INFO @ Sat, 03 Apr 2021 09:50:48: 7000000 INFO @ Sat, 03 Apr 2021 09:50:51: 15000000 INFO @ Sat, 03 Apr 2021 09:50:52: 11000000 INFO @ Sat, 03 Apr 2021 09:50:56: 8000000 INFO @ Sat, 03 Apr 2021 09:50:59: 16000000 INFO @ Sat, 03 Apr 2021 09:51:00: 12000000 INFO @ Sat, 03 Apr 2021 09:51:04: 9000000 INFO @ Sat, 03 Apr 2021 09:51:07: 17000000 INFO @ Sat, 03 Apr 2021 09:51:08: 13000000 INFO @ Sat, 03 Apr 2021 09:51:12: 10000000 INFO @ Sat, 03 Apr 2021 09:51:15: 18000000 INFO @ Sat, 03 Apr 2021 09:51:16: 14000000 INFO @ Sat, 03 Apr 2021 09:51:20: 11000000 INFO @ Sat, 03 Apr 2021 09:51:24: 19000000 INFO @ Sat, 03 Apr 2021 09:51:24: 15000000 INFO @ Sat, 03 Apr 2021 09:51:28: 12000000 INFO @ Sat, 03 Apr 2021 09:51:32: 20000000 INFO @ Sat, 03 Apr 2021 09:51:32: 16000000 INFO @ Sat, 03 Apr 2021 09:51:36: 13000000 INFO @ Sat, 03 Apr 2021 09:51:40: 21000000 INFO @ Sat, 03 Apr 2021 09:51:40: 17000000 INFO @ Sat, 03 Apr 2021 09:51:44: 14000000 INFO @ Sat, 03 Apr 2021 09:51:48: 22000000 INFO @ Sat, 03 Apr 2021 09:51:48: 18000000 INFO @ Sat, 03 Apr 2021 09:51:51: 15000000 INFO @ Sat, 03 Apr 2021 09:51:56: 19000000 INFO @ Sat, 03 Apr 2021 09:51:56: 23000000 INFO @ Sat, 03 Apr 2021 09:51:59: 16000000 INFO @ Sat, 03 Apr 2021 09:52:05: 20000000 INFO @ Sat, 03 Apr 2021 09:52:05: 24000000 INFO @ Sat, 03 Apr 2021 09:52:08: 17000000 INFO @ Sat, 03 Apr 2021 09:52:14: 21000000 INFO @ Sat, 03 Apr 2021 09:52:14: 25000000 INFO @ Sat, 03 Apr 2021 09:52:16: 18000000 INFO @ Sat, 03 Apr 2021 09:52:22: 22000000 INFO @ Sat, 03 Apr 2021 09:52:23: 26000000 INFO @ Sat, 03 Apr 2021 09:52:25: 19000000 INFO @ Sat, 03 Apr 2021 09:52:31: 23000000 INFO @ Sat, 03 Apr 2021 09:52:31: 27000000 INFO @ Sat, 03 Apr 2021 09:52:34: 20000000 INFO @ Sat, 03 Apr 2021 09:52:39: 24000000 INFO @ Sat, 03 Apr 2021 09:52:40: 28000000 INFO @ Sat, 03 Apr 2021 09:52:43: 21000000 INFO @ Sat, 03 Apr 2021 09:52:48: 29000000 INFO @ Sat, 03 Apr 2021 09:52:48: 25000000 INFO @ Sat, 03 Apr 2021 09:52:52: 22000000 INFO @ Sat, 03 Apr 2021 09:52:57: 30000000 INFO @ Sat, 03 Apr 2021 09:52:58: 26000000 INFO @ Sat, 03 Apr 2021 09:53:01: 23000000 INFO @ Sat, 03 Apr 2021 09:53:06: 27000000 INFO @ Sat, 03 Apr 2021 09:53:06: 31000000 INFO @ Sat, 03 Apr 2021 09:53:10: 24000000 INFO @ Sat, 03 Apr 2021 09:53:14: 28000000 INFO @ Sat, 03 Apr 2021 09:53:15: 32000000 INFO @ Sat, 03 Apr 2021 09:53:18: 25000000 INFO @ Sat, 03 Apr 2021 09:53:23: 29000000 INFO @ Sat, 03 Apr 2021 09:53:23: 33000000 INFO @ Sat, 03 Apr 2021 09:53:27: 26000000 INFO @ Sat, 03 Apr 2021 09:53:32: 30000000 INFO @ Sat, 03 Apr 2021 09:53:32: 34000000 INFO @ Sat, 03 Apr 2021 09:53:36: 27000000 INFO @ Sat, 03 Apr 2021 09:53:40: 35000000 INFO @ Sat, 03 Apr 2021 09:53:40: 31000000 INFO @ Sat, 03 Apr 2021 09:53:44: 28000000 INFO @ Sat, 03 Apr 2021 09:53:49: 36000000 INFO @ Sat, 03 Apr 2021 09:53:49: 32000000 INFO @ Sat, 03 Apr 2021 09:53:53: 29000000 INFO @ Sat, 03 Apr 2021 09:53:57: 37000000 INFO @ Sat, 03 Apr 2021 09:53:58: 33000000 INFO @ Sat, 03 Apr 2021 09:54:02: 30000000 INFO @ Sat, 03 Apr 2021 09:54:06: 38000000 INFO @ Sat, 03 Apr 2021 09:54:06: 34000000 INFO @ Sat, 03 Apr 2021 09:54:10: 31000000 INFO @ Sat, 03 Apr 2021 09:54:14: 39000000 INFO @ Sat, 03 Apr 2021 09:54:14: 35000000 INFO @ Sat, 03 Apr 2021 09:54:18: 32000000 INFO @ Sat, 03 Apr 2021 09:54:22: 40000000 INFO @ Sat, 03 Apr 2021 09:54:22: 36000000 INFO @ Sat, 03 Apr 2021 09:54:26: 33000000 INFO @ Sat, 03 Apr 2021 09:54:30: 41000000 INFO @ Sat, 03 Apr 2021 09:54:30: 37000000 INFO @ Sat, 03 Apr 2021 09:54:35: 34000000 INFO @ Sat, 03 Apr 2021 09:54:38: 42000000 INFO @ Sat, 03 Apr 2021 09:54:39: 38000000 INFO @ Sat, 03 Apr 2021 09:54:43: 35000000 INFO @ Sat, 03 Apr 2021 09:54:46: 43000000 INFO @ Sat, 03 Apr 2021 09:54:47: 39000000 INFO @ Sat, 03 Apr 2021 09:54:51: 36000000 INFO @ Sat, 03 Apr 2021 09:54:54: 44000000 INFO @ Sat, 03 Apr 2021 09:54:55: 40000000 INFO @ Sat, 03 Apr 2021 09:54:59: 37000000 INFO @ Sat, 03 Apr 2021 09:55:02: 45000000 INFO @ Sat, 03 Apr 2021 09:55:03: 41000000 INFO @ Sat, 03 Apr 2021 09:55:06: 38000000 INFO @ Sat, 03 Apr 2021 09:55:10: 46000000 INFO @ Sat, 03 Apr 2021 09:55:11: 42000000 INFO @ Sat, 03 Apr 2021 09:55:14: 39000000 INFO @ Sat, 03 Apr 2021 09:55:18: 47000000 INFO @ Sat, 03 Apr 2021 09:55:19: 43000000 INFO @ Sat, 03 Apr 2021 09:55:22: 40000000 INFO @ Sat, 03 Apr 2021 09:55:27: 48000000 INFO @ Sat, 03 Apr 2021 09:55:28: 44000000 INFO @ Sat, 03 Apr 2021 09:55:30: 41000000 INFO @ Sat, 03 Apr 2021 09:55:37: 45000000 INFO @ Sat, 03 Apr 2021 09:55:37: 49000000 INFO @ Sat, 03 Apr 2021 09:55:39: 42000000 INFO @ Sat, 03 Apr 2021 09:55:45: 46000000 INFO @ Sat, 03 Apr 2021 09:55:46: 50000000 INFO @ Sat, 03 Apr 2021 09:55:48: 43000000 INFO @ Sat, 03 Apr 2021 09:55:53: 47000000 INFO @ Sat, 03 Apr 2021 09:55:55: 51000000 INFO @ Sat, 03 Apr 2021 09:55:56: 44000000 INFO @ Sat, 03 Apr 2021 09:56:01: 48000000 INFO @ Sat, 03 Apr 2021 09:56:04: 45000000 INFO @ Sat, 03 Apr 2021 09:56:05: 52000000 INFO @ Sat, 03 Apr 2021 09:56:09: 49000000 INFO @ Sat, 03 Apr 2021 09:56:11: 46000000 INFO @ Sat, 03 Apr 2021 09:56:15: 53000000 INFO @ Sat, 03 Apr 2021 09:56:17: 50000000 INFO @ Sat, 03 Apr 2021 09:56:19: 47000000 INFO @ Sat, 03 Apr 2021 09:56:25: 54000000 INFO @ Sat, 03 Apr 2021 09:56:26: 51000000 INFO @ Sat, 03 Apr 2021 09:56:28: 48000000 INFO @ Sat, 03 Apr 2021 09:56:34: 52000000 INFO @ Sat, 03 Apr 2021 09:56:35: 55000000 INFO @ Sat, 03 Apr 2021 09:56:36: 49000000 INFO @ Sat, 03 Apr 2021 09:56:42: 53000000 INFO @ Sat, 03 Apr 2021 09:56:43: 56000000 INFO @ Sat, 03 Apr 2021 09:56:44: 50000000 INFO @ Sat, 03 Apr 2021 09:56:50: 54000000 INFO @ Sat, 03 Apr 2021 09:56:52: 57000000 INFO @ Sat, 03 Apr 2021 09:56:52: 51000000 INFO @ Sat, 03 Apr 2021 09:57:00: 55000000 INFO @ Sat, 03 Apr 2021 09:57:01: 52000000 INFO @ Sat, 03 Apr 2021 09:57:04: 58000000 INFO @ Sat, 03 Apr 2021 09:57:09: 56000000 INFO @ Sat, 03 Apr 2021 09:57:12: 53000000 INFO @ Sat, 03 Apr 2021 09:57:16: 59000000 INFO @ Sat, 03 Apr 2021 09:57:17: 57000000 INFO @ Sat, 03 Apr 2021 09:57:20: 54000000 INFO @ Sat, 03 Apr 2021 09:57:26: 58000000 INFO @ Sat, 03 Apr 2021 09:57:26: 60000000 INFO @ Sat, 03 Apr 2021 09:57:28: 55000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 09:57:34: 59000000 INFO @ Sat, 03 Apr 2021 09:57:36: 61000000 INFO @ Sat, 03 Apr 2021 09:57:36: 56000000 INFO @ Sat, 03 Apr 2021 09:57:42: 60000000 INFO @ Sat, 03 Apr 2021 09:57:44: 57000000 INFO @ Sat, 03 Apr 2021 09:57:47: 62000000 INFO @ Sat, 03 Apr 2021 09:57:50: 61000000 INFO @ Sat, 03 Apr 2021 09:57:53: 58000000 INFO @ Sat, 03 Apr 2021 09:57:57: 63000000 INFO @ Sat, 03 Apr 2021 09:57:58: 62000000 INFO @ Sat, 03 Apr 2021 09:58:01: 59000000 INFO @ Sat, 03 Apr 2021 09:58:06: 63000000 INFO @ Sat, 03 Apr 2021 09:58:08: 64000000 INFO @ Sat, 03 Apr 2021 09:58:09: 60000000 INFO @ Sat, 03 Apr 2021 09:58:15: 64000000 INFO @ Sat, 03 Apr 2021 09:58:17: 61000000 INFO @ Sat, 03 Apr 2021 09:58:19: 65000000 INFO @ Sat, 03 Apr 2021 09:58:23: 65000000 INFO @ Sat, 03 Apr 2021 09:58:25: 62000000 INFO @ Sat, 03 Apr 2021 09:58:30: 66000000 INFO @ Sat, 03 Apr 2021 09:58:31: 66000000 INFO @ Sat, 03 Apr 2021 09:58:33: 63000000 INFO @ Sat, 03 Apr 2021 09:58:39: 67000000 INFO @ Sat, 03 Apr 2021 09:58:40: 67000000 INFO @ Sat, 03 Apr 2021 09:58:41: 64000000 INFO @ Sat, 03 Apr 2021 09:58:47: 68000000 INFO @ Sat, 03 Apr 2021 09:58:49: 65000000 INFO @ Sat, 03 Apr 2021 09:58:50: 68000000 INFO @ Sat, 03 Apr 2021 09:58:53: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:58:53: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:58:53: #1 total tags in treatment: 27127082 INFO @ Sat, 03 Apr 2021 09:58:53: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:58:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:58:54: #1 tags after filtering in treatment: 18173941 INFO @ Sat, 03 Apr 2021 09:58:54: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 09:58:54: #1 finished! INFO @ Sat, 03 Apr 2021 09:58:54: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:58:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:58:55: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 09:58:55: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 09:58:55: start model_add_line... INFO @ Sat, 03 Apr 2021 09:58:55: start X-correlation... INFO @ Sat, 03 Apr 2021 09:58:55: end of X-cor INFO @ Sat, 03 Apr 2021 09:58:55: #2 finished! INFO @ Sat, 03 Apr 2021 09:58:55: #2 predicted fragment length is 83 bps INFO @ Sat, 03 Apr 2021 09:58:55: #2 alternative fragment length(s) may be 4,83,598 bps INFO @ Sat, 03 Apr 2021 09:58:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.10_model.r WARNING @ Sat, 03 Apr 2021 09:58:55: #2 Since the d (83) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:58:55: #2 You may need to consider one of the other alternative d(s): 4,83,598 WARNING @ Sat, 03 Apr 2021 09:58:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:58:55: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:58:55: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:58:57: 66000000 INFO @ Sat, 03 Apr 2021 09:58:57: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:58:57: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:58:57: #1 total tags in treatment: 27127082 INFO @ Sat, 03 Apr 2021 09:58:57: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:58:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:58:58: #1 tags after filtering in treatment: 18173941 INFO @ Sat, 03 Apr 2021 09:58:58: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 09:58:58: #1 finished! INFO @ Sat, 03 Apr 2021 09:58:58: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:58:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:58:59: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 09:58:59: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 09:58:59: start model_add_line... INFO @ Sat, 03 Apr 2021 09:58:59: start X-correlation... INFO @ Sat, 03 Apr 2021 09:58:59: end of X-cor INFO @ Sat, 03 Apr 2021 09:58:59: #2 finished! INFO @ Sat, 03 Apr 2021 09:58:59: #2 predicted fragment length is 83 bps INFO @ Sat, 03 Apr 2021 09:58:59: #2 alternative fragment length(s) may be 4,83,598 bps INFO @ Sat, 03 Apr 2021 09:58:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.05_model.r WARNING @ Sat, 03 Apr 2021 09:58:59: #2 Since the d (83) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:58:59: #2 You may need to consider one of the other alternative d(s): 4,83,598 WARNING @ Sat, 03 Apr 2021 09:58:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:58:59: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:58:59: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 09:59:04: 67000000 INFO @ Sat, 03 Apr 2021 09:59:11: 68000000 INFO @ Sat, 03 Apr 2021 09:59:17: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 09:59:17: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 09:59:17: #1 total tags in treatment: 27127082 INFO @ Sat, 03 Apr 2021 09:59:17: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 09:59:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 09:59:17: #1 tags after filtering in treatment: 18173941 INFO @ Sat, 03 Apr 2021 09:59:17: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 09:59:17: #1 finished! INFO @ Sat, 03 Apr 2021 09:59:17: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 09:59:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 09:59:18: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 09:59:18: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 09:59:18: start model_add_line... INFO @ Sat, 03 Apr 2021 09:59:19: start X-correlation... INFO @ Sat, 03 Apr 2021 09:59:19: end of X-cor INFO @ Sat, 03 Apr 2021 09:59:19: #2 finished! INFO @ Sat, 03 Apr 2021 09:59:19: #2 predicted fragment length is 83 bps INFO @ Sat, 03 Apr 2021 09:59:19: #2 alternative fragment length(s) may be 4,83,598 bps INFO @ Sat, 03 Apr 2021 09:59:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.20_model.r WARNING @ Sat, 03 Apr 2021 09:59:19: #2 Since the d (83) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 09:59:19: #2 You may need to consider one of the other alternative d(s): 4,83,598 WARNING @ Sat, 03 Apr 2021 09:59:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 09:59:19: #3 Call peaks... INFO @ Sat, 03 Apr 2021 09:59:19: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 09:59:28: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:59:32: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:59:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.10_peaks.xls INFO @ Sat, 03 Apr 2021 09:59:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:59:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.10_summits.bed INFO @ Sat, 03 Apr 2021 09:59:46: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5633 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 09:59:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.05_peaks.xls INFO @ Sat, 03 Apr 2021 09:59:51: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 09:59:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 09:59:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.05_summits.bed INFO @ Sat, 03 Apr 2021 09:59:51: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (10186 records, 4 fields): 559 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:00:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.20_peaks.xls INFO @ Sat, 03 Apr 2021 10:00:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:00:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX3029124/SRX3029124.20_summits.bed INFO @ Sat, 03 Apr 2021 10:00:09: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2083 records, 4 fields): 4 millis CompletedMACS2peakCalling