Job ID = 6626595 SRX = SRX3008772 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:52 39732741 reads; of these: 39732741 (100.00%) were unpaired; of these: 645282 (1.62%) aligned 0 times 33453577 (84.20%) aligned exactly 1 time 5633882 (14.18%) aligned >1 times 98.38% overall alignment rate Time searching: 00:08:52 Overall time: 00:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6881361 / 39087459 = 0.1761 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:04: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:04: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:10: 1000000 INFO @ Tue, 14 Jul 2020 08:00:15: 2000000 INFO @ Tue, 14 Jul 2020 08:00:21: 3000000 INFO @ Tue, 14 Jul 2020 08:00:27: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:32: 5000000 INFO @ Tue, 14 Jul 2020 08:00:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:39: 6000000 INFO @ Tue, 14 Jul 2020 08:00:39: 1000000 INFO @ Tue, 14 Jul 2020 08:00:45: 7000000 INFO @ Tue, 14 Jul 2020 08:00:46: 2000000 INFO @ Tue, 14 Jul 2020 08:00:52: 8000000 INFO @ Tue, 14 Jul 2020 08:00:52: 3000000 INFO @ Tue, 14 Jul 2020 08:00:58: 9000000 INFO @ Tue, 14 Jul 2020 08:00:58: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:01:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:01:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:01:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:04: 10000000 INFO @ Tue, 14 Jul 2020 08:01:05: 5000000 INFO @ Tue, 14 Jul 2020 08:01:10: 1000000 INFO @ Tue, 14 Jul 2020 08:01:11: 11000000 INFO @ Tue, 14 Jul 2020 08:01:11: 6000000 INFO @ Tue, 14 Jul 2020 08:01:16: 2000000 INFO @ Tue, 14 Jul 2020 08:01:18: 12000000 INFO @ Tue, 14 Jul 2020 08:01:18: 7000000 INFO @ Tue, 14 Jul 2020 08:01:23: 3000000 INFO @ Tue, 14 Jul 2020 08:01:24: 13000000 INFO @ Tue, 14 Jul 2020 08:01:24: 8000000 INFO @ Tue, 14 Jul 2020 08:01:29: 4000000 INFO @ Tue, 14 Jul 2020 08:01:31: 14000000 INFO @ Tue, 14 Jul 2020 08:01:31: 9000000 INFO @ Tue, 14 Jul 2020 08:01:36: 5000000 INFO @ Tue, 14 Jul 2020 08:01:37: 15000000 INFO @ Tue, 14 Jul 2020 08:01:38: 10000000 INFO @ Tue, 14 Jul 2020 08:01:42: 6000000 INFO @ Tue, 14 Jul 2020 08:01:44: 16000000 INFO @ Tue, 14 Jul 2020 08:01:44: 11000000 INFO @ Tue, 14 Jul 2020 08:01:49: 7000000 INFO @ Tue, 14 Jul 2020 08:01:50: 17000000 INFO @ Tue, 14 Jul 2020 08:01:51: 12000000 INFO @ Tue, 14 Jul 2020 08:01:55: 8000000 INFO @ Tue, 14 Jul 2020 08:01:57: 18000000 INFO @ Tue, 14 Jul 2020 08:01:57: 13000000 INFO @ Tue, 14 Jul 2020 08:02:02: 9000000 INFO @ Tue, 14 Jul 2020 08:02:03: 19000000 INFO @ Tue, 14 Jul 2020 08:02:04: 14000000 INFO @ Tue, 14 Jul 2020 08:02:08: 10000000 INFO @ Tue, 14 Jul 2020 08:02:10: 20000000 INFO @ Tue, 14 Jul 2020 08:02:10: 15000000 INFO @ Tue, 14 Jul 2020 08:02:15: 11000000 INFO @ Tue, 14 Jul 2020 08:02:17: 21000000 INFO @ Tue, 14 Jul 2020 08:02:17: 16000000 INFO @ Tue, 14 Jul 2020 08:02:21: 12000000 INFO @ Tue, 14 Jul 2020 08:02:23: 22000000 INFO @ Tue, 14 Jul 2020 08:02:23: 17000000 INFO @ Tue, 14 Jul 2020 08:02:28: 13000000 INFO @ Tue, 14 Jul 2020 08:02:30: 23000000 INFO @ Tue, 14 Jul 2020 08:02:30: 18000000 INFO @ Tue, 14 Jul 2020 08:02:34: 14000000 INFO @ Tue, 14 Jul 2020 08:02:36: 24000000 INFO @ Tue, 14 Jul 2020 08:02:37: 19000000 INFO @ Tue, 14 Jul 2020 08:02:41: 15000000 INFO @ Tue, 14 Jul 2020 08:02:43: 25000000 INFO @ Tue, 14 Jul 2020 08:02:43: 20000000 INFO @ Tue, 14 Jul 2020 08:02:47: 16000000 INFO @ Tue, 14 Jul 2020 08:02:49: 26000000 INFO @ Tue, 14 Jul 2020 08:02:50: 21000000 INFO @ Tue, 14 Jul 2020 08:02:54: 17000000 INFO @ Tue, 14 Jul 2020 08:02:56: 27000000 INFO @ Tue, 14 Jul 2020 08:02:56: 22000000 INFO @ Tue, 14 Jul 2020 08:03:01: 18000000 INFO @ Tue, 14 Jul 2020 08:03:03: 28000000 INFO @ Tue, 14 Jul 2020 08:03:03: 23000000 INFO @ Tue, 14 Jul 2020 08:03:07: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:03:09: 29000000 INFO @ Tue, 14 Jul 2020 08:03:09: 24000000 INFO @ Tue, 14 Jul 2020 08:03:14: 20000000 INFO @ Tue, 14 Jul 2020 08:03:16: 30000000 INFO @ Tue, 14 Jul 2020 08:03:16: 25000000 INFO @ Tue, 14 Jul 2020 08:03:20: 21000000 INFO @ Tue, 14 Jul 2020 08:03:22: 26000000 INFO @ Tue, 14 Jul 2020 08:03:22: 31000000 INFO @ Tue, 14 Jul 2020 08:03:27: 22000000 INFO @ Tue, 14 Jul 2020 08:03:29: 27000000 INFO @ Tue, 14 Jul 2020 08:03:29: 32000000 INFO @ Tue, 14 Jul 2020 08:03:30: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:03:30: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:03:30: #1 total tags in treatment: 32206098 INFO @ Tue, 14 Jul 2020 08:03:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:31: #1 tags after filtering in treatment: 32206098 INFO @ Tue, 14 Jul 2020 08:03:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:31: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:33: #2 number of paired peaks: 22 WARNING @ Tue, 14 Jul 2020 08:03:33: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:03:33: 23000000 INFO @ Tue, 14 Jul 2020 08:03:35: 28000000 INFO @ Tue, 14 Jul 2020 08:03:39: 24000000 INFO @ Tue, 14 Jul 2020 08:03:42: 29000000 INFO @ Tue, 14 Jul 2020 08:03:46: 25000000 INFO @ Tue, 14 Jul 2020 08:03:48: 30000000 INFO @ Tue, 14 Jul 2020 08:03:52: 26000000 INFO @ Tue, 14 Jul 2020 08:03:54: 31000000 INFO @ Tue, 14 Jul 2020 08:03:58: 27000000 INFO @ Tue, 14 Jul 2020 08:04:01: 32000000 INFO @ Tue, 14 Jul 2020 08:04:02: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:04:02: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:04:02: #1 total tags in treatment: 32206098 INFO @ Tue, 14 Jul 2020 08:04:02: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:04:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:04:03: #1 tags after filtering in treatment: 32206098 INFO @ Tue, 14 Jul 2020 08:04:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:04:03: #1 finished! INFO @ Tue, 14 Jul 2020 08:04:03: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:04:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:04:05: #2 number of paired peaks: 22 WARNING @ Tue, 14 Jul 2020 08:04:05: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:04:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:04:05: 28000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:04:11: 29000000 INFO @ Tue, 14 Jul 2020 08:04:17: 30000000 INFO @ Tue, 14 Jul 2020 08:04:22: 31000000 INFO @ Tue, 14 Jul 2020 08:04:28: 32000000 INFO @ Tue, 14 Jul 2020 08:04:30: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:04:30: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:04:30: #1 total tags in treatment: 32206098 INFO @ Tue, 14 Jul 2020 08:04:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:04:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:04:30: #1 tags after filtering in treatment: 32206098 INFO @ Tue, 14 Jul 2020 08:04:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:04:30: #1 finished! INFO @ Tue, 14 Jul 2020 08:04:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:04:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:04:32: #2 number of paired peaks: 22 WARNING @ Tue, 14 Jul 2020 08:04:32: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:04:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008772/SRX3008772.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling