Job ID = 6626594 SRX = SRX3008770 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:10 40235808 reads; of these: 40235808 (100.00%) were unpaired; of these: 401075 (1.00%) aligned 0 times 33826208 (84.07%) aligned exactly 1 time 6008525 (14.93%) aligned >1 times 99.00% overall alignment rate Time searching: 00:08:10 Overall time: 00:08:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6784622 / 39834733 = 0.1703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:58:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:58:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:58:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:58:59: 1000000 INFO @ Tue, 14 Jul 2020 07:59:04: 2000000 INFO @ Tue, 14 Jul 2020 07:59:10: 3000000 INFO @ Tue, 14 Jul 2020 07:59:15: 4000000 INFO @ Tue, 14 Jul 2020 07:59:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:59:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:59:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:59:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:59:26: 6000000 INFO @ Tue, 14 Jul 2020 07:59:31: 1000000 INFO @ Tue, 14 Jul 2020 07:59:32: 7000000 INFO @ Tue, 14 Jul 2020 07:59:37: 2000000 INFO @ Tue, 14 Jul 2020 07:59:39: 8000000 INFO @ Tue, 14 Jul 2020 07:59:44: 3000000 INFO @ Tue, 14 Jul 2020 07:59:45: 9000000 INFO @ Tue, 14 Jul 2020 07:59:51: 4000000 INFO @ Tue, 14 Jul 2020 07:59:51: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:59:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:59:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:59:57: 11000000 INFO @ Tue, 14 Jul 2020 07:59:58: 5000000 INFO @ Tue, 14 Jul 2020 08:00:01: 1000000 INFO @ Tue, 14 Jul 2020 08:00:04: 12000000 INFO @ Tue, 14 Jul 2020 08:00:05: 6000000 INFO @ Tue, 14 Jul 2020 08:00:08: 2000000 INFO @ Tue, 14 Jul 2020 08:00:10: 13000000 INFO @ Tue, 14 Jul 2020 08:00:12: 7000000 INFO @ Tue, 14 Jul 2020 08:00:15: 3000000 INFO @ Tue, 14 Jul 2020 08:00:16: 14000000 INFO @ Tue, 14 Jul 2020 08:00:18: 8000000 INFO @ Tue, 14 Jul 2020 08:00:22: 4000000 INFO @ Tue, 14 Jul 2020 08:00:22: 15000000 INFO @ Tue, 14 Jul 2020 08:00:25: 9000000 INFO @ Tue, 14 Jul 2020 08:00:29: 5000000 INFO @ Tue, 14 Jul 2020 08:00:29: 16000000 INFO @ Tue, 14 Jul 2020 08:00:32: 10000000 INFO @ Tue, 14 Jul 2020 08:00:35: 17000000 INFO @ Tue, 14 Jul 2020 08:00:36: 6000000 INFO @ Tue, 14 Jul 2020 08:00:39: 11000000 INFO @ Tue, 14 Jul 2020 08:00:42: 18000000 INFO @ Tue, 14 Jul 2020 08:00:43: 7000000 INFO @ Tue, 14 Jul 2020 08:00:46: 12000000 INFO @ Tue, 14 Jul 2020 08:00:48: 19000000 INFO @ Tue, 14 Jul 2020 08:00:49: 8000000 INFO @ Tue, 14 Jul 2020 08:00:52: 13000000 INFO @ Tue, 14 Jul 2020 08:00:54: 20000000 INFO @ Tue, 14 Jul 2020 08:00:56: 9000000 INFO @ Tue, 14 Jul 2020 08:00:59: 14000000 INFO @ Tue, 14 Jul 2020 08:01:00: 21000000 INFO @ Tue, 14 Jul 2020 08:01:03: 10000000 INFO @ Tue, 14 Jul 2020 08:01:06: 15000000 INFO @ Tue, 14 Jul 2020 08:01:06: 22000000 INFO @ Tue, 14 Jul 2020 08:01:10: 11000000 INFO @ Tue, 14 Jul 2020 08:01:13: 23000000 INFO @ Tue, 14 Jul 2020 08:01:13: 16000000 INFO @ Tue, 14 Jul 2020 08:01:17: 12000000 INFO @ Tue, 14 Jul 2020 08:01:19: 24000000 INFO @ Tue, 14 Jul 2020 08:01:20: 17000000 INFO @ Tue, 14 Jul 2020 08:01:24: 13000000 INFO @ Tue, 14 Jul 2020 08:01:25: 25000000 INFO @ Tue, 14 Jul 2020 08:01:27: 18000000 INFO @ Tue, 14 Jul 2020 08:01:30: 14000000 INFO @ Tue, 14 Jul 2020 08:01:31: 26000000 INFO @ Tue, 14 Jul 2020 08:01:34: 19000000 INFO @ Tue, 14 Jul 2020 08:01:37: 27000000 INFO @ Tue, 14 Jul 2020 08:01:38: 15000000 INFO @ Tue, 14 Jul 2020 08:01:41: 20000000 INFO @ Tue, 14 Jul 2020 08:01:44: 28000000 INFO @ Tue, 14 Jul 2020 08:01:45: 16000000 INFO @ Tue, 14 Jul 2020 08:01:48: 21000000 INFO @ Tue, 14 Jul 2020 08:01:50: 29000000 INFO @ Tue, 14 Jul 2020 08:01:52: 17000000 INFO @ Tue, 14 Jul 2020 08:01:54: 22000000 INFO @ Tue, 14 Jul 2020 08:01:56: 30000000 INFO @ Tue, 14 Jul 2020 08:01:59: 18000000 INFO @ Tue, 14 Jul 2020 08:02:01: 23000000 INFO @ Tue, 14 Jul 2020 08:02:02: 31000000 INFO @ Tue, 14 Jul 2020 08:02:06: 19000000 INFO @ Tue, 14 Jul 2020 08:02:08: 24000000 INFO @ Tue, 14 Jul 2020 08:02:08: 32000000 INFO @ Tue, 14 Jul 2020 08:02:13: 20000000 INFO @ Tue, 14 Jul 2020 08:02:15: 25000000 INFO @ Tue, 14 Jul 2020 08:02:15: 33000000 INFO @ Tue, 14 Jul 2020 08:02:15: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:02:15: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:02:15: #1 total tags in treatment: 33050111 INFO @ Tue, 14 Jul 2020 08:02:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:02:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:02:16: #1 tags after filtering in treatment: 33050111 INFO @ Tue, 14 Jul 2020 08:02:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:02:16: #1 finished! INFO @ Tue, 14 Jul 2020 08:02:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:02:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:02:18: #2 number of paired peaks: 23 WARNING @ Tue, 14 Jul 2020 08:02:18: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:02:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:02:20: 21000000 INFO @ Tue, 14 Jul 2020 08:02:22: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:02:26: 22000000 INFO @ Tue, 14 Jul 2020 08:02:28: 27000000 INFO @ Tue, 14 Jul 2020 08:02:33: 23000000 INFO @ Tue, 14 Jul 2020 08:02:35: 28000000 INFO @ Tue, 14 Jul 2020 08:02:40: 24000000 INFO @ Tue, 14 Jul 2020 08:02:42: 29000000 INFO @ Tue, 14 Jul 2020 08:02:47: 25000000 INFO @ Tue, 14 Jul 2020 08:02:48: 30000000 INFO @ Tue, 14 Jul 2020 08:02:53: 26000000 INFO @ Tue, 14 Jul 2020 08:02:55: 31000000 INFO @ Tue, 14 Jul 2020 08:03:00: 27000000 INFO @ Tue, 14 Jul 2020 08:03:02: 32000000 INFO @ Tue, 14 Jul 2020 08:03:07: 28000000 INFO @ Tue, 14 Jul 2020 08:03:08: 33000000 INFO @ Tue, 14 Jul 2020 08:03:09: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:03:09: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:03:09: #1 total tags in treatment: 33050111 INFO @ Tue, 14 Jul 2020 08:03:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:10: #1 tags after filtering in treatment: 33050111 INFO @ Tue, 14 Jul 2020 08:03:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:10: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:10: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:12: #2 number of paired peaks: 23 WARNING @ Tue, 14 Jul 2020 08:03:12: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:03:13: 29000000 INFO @ Tue, 14 Jul 2020 08:03:20: 30000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:03:26: 31000000 INFO @ Tue, 14 Jul 2020 08:03:33: 32000000 INFO @ Tue, 14 Jul 2020 08:03:39: 33000000 INFO @ Tue, 14 Jul 2020 08:03:40: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:03:40: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:03:40: #1 total tags in treatment: 33050111 INFO @ Tue, 14 Jul 2020 08:03:40: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:40: #1 tags after filtering in treatment: 33050111 INFO @ Tue, 14 Jul 2020 08:03:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:40: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:40: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:42: #2 number of paired peaks: 23 WARNING @ Tue, 14 Jul 2020 08:03:42: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008770/SRX3008770.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling