Job ID = 6626591 SRX = SRX3008769 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:51 42913454 reads; of these: 42913454 (100.00%) were unpaired; of these: 529248 (1.23%) aligned 0 times 35925921 (83.72%) aligned exactly 1 time 6458285 (15.05%) aligned >1 times 98.77% overall alignment rate Time searching: 00:09:51 Overall time: 00:09:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7521846 / 42384206 = 0.1775 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:33: 1000000 INFO @ Tue, 14 Jul 2020 08:00:37: 2000000 INFO @ Tue, 14 Jul 2020 08:00:42: 3000000 INFO @ Tue, 14 Jul 2020 08:00:47: 4000000 INFO @ Tue, 14 Jul 2020 08:00:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:57: 6000000 INFO @ Tue, 14 Jul 2020 08:00:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:58: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:58: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:02: 7000000 INFO @ Tue, 14 Jul 2020 08:01:03: 1000000 INFO @ Tue, 14 Jul 2020 08:01:07: 8000000 INFO @ Tue, 14 Jul 2020 08:01:08: 2000000 INFO @ Tue, 14 Jul 2020 08:01:12: 9000000 INFO @ Tue, 14 Jul 2020 08:01:13: 3000000 INFO @ Tue, 14 Jul 2020 08:01:17: 10000000 INFO @ Tue, 14 Jul 2020 08:01:18: 4000000 INFO @ Tue, 14 Jul 2020 08:01:22: 11000000 INFO @ Tue, 14 Jul 2020 08:01:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:01:27: 12000000 INFO @ Tue, 14 Jul 2020 08:01:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:01:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:01:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:28: 6000000 INFO @ Tue, 14 Jul 2020 08:01:32: 13000000 INFO @ Tue, 14 Jul 2020 08:01:33: 7000000 INFO @ Tue, 14 Jul 2020 08:01:35: 1000000 INFO @ Tue, 14 Jul 2020 08:01:37: 14000000 INFO @ Tue, 14 Jul 2020 08:01:38: 8000000 INFO @ Tue, 14 Jul 2020 08:01:41: 2000000 INFO @ Tue, 14 Jul 2020 08:01:42: 15000000 INFO @ Tue, 14 Jul 2020 08:01:43: 9000000 INFO @ Tue, 14 Jul 2020 08:01:47: 16000000 INFO @ Tue, 14 Jul 2020 08:01:48: 10000000 INFO @ Tue, 14 Jul 2020 08:01:48: 3000000 INFO @ Tue, 14 Jul 2020 08:01:53: 17000000 INFO @ Tue, 14 Jul 2020 08:01:53: 11000000 INFO @ Tue, 14 Jul 2020 08:01:55: 4000000 INFO @ Tue, 14 Jul 2020 08:01:58: 18000000 INFO @ Tue, 14 Jul 2020 08:01:58: 12000000 INFO @ Tue, 14 Jul 2020 08:02:01: 5000000 INFO @ Tue, 14 Jul 2020 08:02:03: 13000000 INFO @ Tue, 14 Jul 2020 08:02:03: 19000000 INFO @ Tue, 14 Jul 2020 08:02:07: 6000000 INFO @ Tue, 14 Jul 2020 08:02:08: 14000000 INFO @ Tue, 14 Jul 2020 08:02:08: 20000000 INFO @ Tue, 14 Jul 2020 08:02:13: 15000000 INFO @ Tue, 14 Jul 2020 08:02:13: 21000000 INFO @ Tue, 14 Jul 2020 08:02:13: 7000000 INFO @ Tue, 14 Jul 2020 08:02:18: 16000000 INFO @ Tue, 14 Jul 2020 08:02:18: 22000000 INFO @ Tue, 14 Jul 2020 08:02:20: 8000000 INFO @ Tue, 14 Jul 2020 08:02:22: 17000000 INFO @ Tue, 14 Jul 2020 08:02:23: 23000000 INFO @ Tue, 14 Jul 2020 08:02:26: 9000000 INFO @ Tue, 14 Jul 2020 08:02:27: 18000000 INFO @ Tue, 14 Jul 2020 08:02:28: 24000000 INFO @ Tue, 14 Jul 2020 08:02:32: 10000000 INFO @ Tue, 14 Jul 2020 08:02:32: 19000000 INFO @ Tue, 14 Jul 2020 08:02:33: 25000000 INFO @ Tue, 14 Jul 2020 08:02:37: 20000000 INFO @ Tue, 14 Jul 2020 08:02:38: 26000000 INFO @ Tue, 14 Jul 2020 08:02:38: 11000000 INFO @ Tue, 14 Jul 2020 08:02:42: 21000000 INFO @ Tue, 14 Jul 2020 08:02:43: 27000000 INFO @ Tue, 14 Jul 2020 08:02:45: 12000000 INFO @ Tue, 14 Jul 2020 08:02:47: 22000000 INFO @ Tue, 14 Jul 2020 08:02:48: 28000000 INFO @ Tue, 14 Jul 2020 08:02:51: 13000000 INFO @ Tue, 14 Jul 2020 08:02:52: 23000000 INFO @ Tue, 14 Jul 2020 08:02:54: 29000000 INFO @ Tue, 14 Jul 2020 08:02:57: 24000000 INFO @ Tue, 14 Jul 2020 08:02:58: 14000000 INFO @ Tue, 14 Jul 2020 08:02:59: 30000000 INFO @ Tue, 14 Jul 2020 08:03:02: 25000000 INFO @ Tue, 14 Jul 2020 08:03:04: 31000000 INFO @ Tue, 14 Jul 2020 08:03:04: 15000000 INFO @ Tue, 14 Jul 2020 08:03:07: 26000000 INFO @ Tue, 14 Jul 2020 08:03:09: 32000000 INFO @ Tue, 14 Jul 2020 08:03:11: 16000000 INFO @ Tue, 14 Jul 2020 08:03:12: 27000000 INFO @ Tue, 14 Jul 2020 08:03:14: 33000000 INFO @ Tue, 14 Jul 2020 08:03:17: 28000000 INFO @ Tue, 14 Jul 2020 08:03:17: 17000000 INFO @ Tue, 14 Jul 2020 08:03:19: 34000000 INFO @ Tue, 14 Jul 2020 08:03:22: 29000000 INFO @ Tue, 14 Jul 2020 08:03:24: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:03:24: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:03:24: #1 total tags in treatment: 34862360 INFO @ Tue, 14 Jul 2020 08:03:24: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:24: 18000000 INFO @ Tue, 14 Jul 2020 08:03:24: #1 tags after filtering in treatment: 34862360 INFO @ Tue, 14 Jul 2020 08:03:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:24: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:24: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:26: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 08:03:26: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:03:27: 30000000 INFO @ Tue, 14 Jul 2020 08:03:30: 19000000 INFO @ Tue, 14 Jul 2020 08:03:32: 31000000 INFO @ Tue, 14 Jul 2020 08:03:36: 20000000 INFO @ Tue, 14 Jul 2020 08:03:37: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:03:42: 33000000 INFO @ Tue, 14 Jul 2020 08:03:43: 21000000 INFO @ Tue, 14 Jul 2020 08:03:47: 34000000 INFO @ Tue, 14 Jul 2020 08:03:49: 22000000 INFO @ Tue, 14 Jul 2020 08:03:51: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:03:51: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:03:51: #1 total tags in treatment: 34862360 INFO @ Tue, 14 Jul 2020 08:03:51: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:52: #1 tags after filtering in treatment: 34862360 INFO @ Tue, 14 Jul 2020 08:03:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:52: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:52: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:54: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 08:03:54: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:03:55: 23000000 INFO @ Tue, 14 Jul 2020 08:04:01: 24000000 INFO @ Tue, 14 Jul 2020 08:04:08: 25000000 INFO @ Tue, 14 Jul 2020 08:04:14: 26000000 INFO @ Tue, 14 Jul 2020 08:04:20: 27000000 INFO @ Tue, 14 Jul 2020 08:04:26: 28000000 INFO @ Tue, 14 Jul 2020 08:04:32: 29000000 INFO @ Tue, 14 Jul 2020 08:04:38: 30000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:04:44: 31000000 INFO @ Tue, 14 Jul 2020 08:04:50: 32000000 INFO @ Tue, 14 Jul 2020 08:04:56: 33000000 INFO @ Tue, 14 Jul 2020 08:05:02: 34000000 INFO @ Tue, 14 Jul 2020 08:05:08: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:05:08: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:05:08: #1 total tags in treatment: 34862360 INFO @ Tue, 14 Jul 2020 08:05:08: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:08: #1 tags after filtering in treatment: 34862360 INFO @ Tue, 14 Jul 2020 08:05:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:08: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:08: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:10: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 08:05:10: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:05:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008769/SRX3008769.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling