Job ID = 6626598 SRX = SRX3008768 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:24 42721018 reads; of these: 42721018 (100.00%) were unpaired; of these: 521419 (1.22%) aligned 0 times 34420130 (80.57%) aligned exactly 1 time 7779469 (18.21%) aligned >1 times 98.78% overall alignment rate Time searching: 00:11:24 Overall time: 00:11:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7899324 / 42199599 = 0.1872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:06:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:06:43: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:06:43: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:06:52: 1000000 INFO @ Tue, 14 Jul 2020 08:07:02: 2000000 INFO @ Tue, 14 Jul 2020 08:07:11: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:07:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:07:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:07:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:07:20: 1000000 INFO @ Tue, 14 Jul 2020 08:07:20: 4000000 INFO @ Tue, 14 Jul 2020 08:07:28: 2000000 INFO @ Tue, 14 Jul 2020 08:07:30: 5000000 INFO @ Tue, 14 Jul 2020 08:07:35: 3000000 INFO @ Tue, 14 Jul 2020 08:07:39: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:07:42: 4000000 INFO @ Tue, 14 Jul 2020 08:07:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:07:43: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:07:43: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:07:48: 7000000 INFO @ Tue, 14 Jul 2020 08:07:49: 5000000 INFO @ Tue, 14 Jul 2020 08:07:50: 1000000 INFO @ Tue, 14 Jul 2020 08:07:57: 6000000 INFO @ Tue, 14 Jul 2020 08:07:57: 2000000 INFO @ Tue, 14 Jul 2020 08:07:58: 8000000 INFO @ Tue, 14 Jul 2020 08:08:03: 3000000 INFO @ Tue, 14 Jul 2020 08:08:04: 7000000 INFO @ Tue, 14 Jul 2020 08:08:08: 9000000 INFO @ Tue, 14 Jul 2020 08:08:10: 4000000 INFO @ Tue, 14 Jul 2020 08:08:11: 8000000 INFO @ Tue, 14 Jul 2020 08:08:17: 5000000 INFO @ Tue, 14 Jul 2020 08:08:18: 10000000 INFO @ Tue, 14 Jul 2020 08:08:19: 9000000 INFO @ Tue, 14 Jul 2020 08:08:23: 6000000 INFO @ Tue, 14 Jul 2020 08:08:26: 10000000 INFO @ Tue, 14 Jul 2020 08:08:28: 11000000 INFO @ Tue, 14 Jul 2020 08:08:30: 7000000 INFO @ Tue, 14 Jul 2020 08:08:34: 11000000 INFO @ Tue, 14 Jul 2020 08:08:36: 8000000 INFO @ Tue, 14 Jul 2020 08:08:37: 12000000 INFO @ Tue, 14 Jul 2020 08:08:41: 12000000 INFO @ Tue, 14 Jul 2020 08:08:43: 9000000 INFO @ Tue, 14 Jul 2020 08:08:46: 13000000 INFO @ Tue, 14 Jul 2020 08:08:48: 13000000 INFO @ Tue, 14 Jul 2020 08:08:50: 10000000 INFO @ Tue, 14 Jul 2020 08:08:56: 14000000 INFO @ Tue, 14 Jul 2020 08:08:56: 14000000 INFO @ Tue, 14 Jul 2020 08:08:56: 11000000 INFO @ Tue, 14 Jul 2020 08:09:03: 12000000 INFO @ Tue, 14 Jul 2020 08:09:04: 15000000 INFO @ Tue, 14 Jul 2020 08:09:05: 15000000 INFO @ Tue, 14 Jul 2020 08:09:09: 13000000 INFO @ Tue, 14 Jul 2020 08:09:11: 16000000 INFO @ Tue, 14 Jul 2020 08:09:14: 16000000 INFO @ Tue, 14 Jul 2020 08:09:16: 14000000 INFO @ Tue, 14 Jul 2020 08:09:18: 17000000 INFO @ Tue, 14 Jul 2020 08:09:23: 15000000 INFO @ Tue, 14 Jul 2020 08:09:23: 17000000 INFO @ Tue, 14 Jul 2020 08:09:25: 18000000 INFO @ Tue, 14 Jul 2020 08:09:29: 16000000 INFO @ Tue, 14 Jul 2020 08:09:32: 18000000 INFO @ Tue, 14 Jul 2020 08:09:32: 19000000 INFO @ Tue, 14 Jul 2020 08:09:36: 17000000 INFO @ Tue, 14 Jul 2020 08:09:40: 20000000 INFO @ Tue, 14 Jul 2020 08:09:41: 19000000 INFO @ Tue, 14 Jul 2020 08:09:42: 18000000 INFO @ Tue, 14 Jul 2020 08:09:47: 21000000 INFO @ Tue, 14 Jul 2020 08:09:49: 19000000 INFO @ Tue, 14 Jul 2020 08:09:49: 20000000 INFO @ Tue, 14 Jul 2020 08:09:54: 22000000 INFO @ Tue, 14 Jul 2020 08:09:55: 20000000 INFO @ Tue, 14 Jul 2020 08:09:58: 21000000 INFO @ Tue, 14 Jul 2020 08:10:01: 23000000 INFO @ Tue, 14 Jul 2020 08:10:02: 21000000 INFO @ Tue, 14 Jul 2020 08:10:07: 22000000 INFO @ Tue, 14 Jul 2020 08:10:08: 22000000 INFO @ Tue, 14 Jul 2020 08:10:08: 24000000 INFO @ Tue, 14 Jul 2020 08:10:16: 25000000 INFO @ Tue, 14 Jul 2020 08:10:16: 23000000 INFO @ Tue, 14 Jul 2020 08:10:16: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:10:22: 24000000 INFO @ Tue, 14 Jul 2020 08:10:23: 26000000 INFO @ Tue, 14 Jul 2020 08:10:25: 24000000 INFO @ Tue, 14 Jul 2020 08:10:29: 25000000 INFO @ Tue, 14 Jul 2020 08:10:30: 27000000 INFO @ Tue, 14 Jul 2020 08:10:34: 25000000 INFO @ Tue, 14 Jul 2020 08:10:35: 26000000 INFO @ Tue, 14 Jul 2020 08:10:37: 28000000 INFO @ Tue, 14 Jul 2020 08:10:42: 27000000 INFO @ Tue, 14 Jul 2020 08:10:43: 26000000 INFO @ Tue, 14 Jul 2020 08:10:44: 29000000 INFO @ Tue, 14 Jul 2020 08:10:48: 28000000 INFO @ Tue, 14 Jul 2020 08:10:51: 30000000 INFO @ Tue, 14 Jul 2020 08:10:52: 27000000 INFO @ Tue, 14 Jul 2020 08:10:55: 29000000 INFO @ Tue, 14 Jul 2020 08:10:58: 31000000 INFO @ Tue, 14 Jul 2020 08:11:01: 30000000 INFO @ Tue, 14 Jul 2020 08:11:01: 28000000 INFO @ Tue, 14 Jul 2020 08:11:06: 32000000 INFO @ Tue, 14 Jul 2020 08:11:08: 31000000 INFO @ Tue, 14 Jul 2020 08:11:10: 29000000 INFO @ Tue, 14 Jul 2020 08:11:13: 33000000 INFO @ Tue, 14 Jul 2020 08:11:14: 32000000 INFO @ Tue, 14 Jul 2020 08:11:17: 30000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:11:20: 34000000 INFO @ Tue, 14 Jul 2020 08:11:21: 33000000 INFO @ Tue, 14 Jul 2020 08:11:22: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:11:22: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:11:22: #1 total tags in treatment: 34300275 INFO @ Tue, 14 Jul 2020 08:11:22: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:11:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:11:23: #1 tags after filtering in treatment: 34300275 INFO @ Tue, 14 Jul 2020 08:11:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:11:23: #1 finished! INFO @ Tue, 14 Jul 2020 08:11:23: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:11:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:11:25: 31000000 INFO @ Tue, 14 Jul 2020 08:11:25: #2 number of paired peaks: 20 WARNING @ Tue, 14 Jul 2020 08:11:25: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:11:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:11:27: 34000000 INFO @ Tue, 14 Jul 2020 08:11:29: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:11:29: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:11:29: #1 total tags in treatment: 34300275 INFO @ Tue, 14 Jul 2020 08:11:29: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:11:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:11:29: #1 tags after filtering in treatment: 34300275 INFO @ Tue, 14 Jul 2020 08:11:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:11:29: #1 finished! INFO @ Tue, 14 Jul 2020 08:11:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:11:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:11:32: #2 number of paired peaks: 20 WARNING @ Tue, 14 Jul 2020 08:11:32: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:11:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:11:32: 32000000 INFO @ Tue, 14 Jul 2020 08:11:39: 33000000 INFO @ Tue, 14 Jul 2020 08:11:45: 34000000 INFO @ Tue, 14 Jul 2020 08:11:48: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:11:48: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:11:48: #1 total tags in treatment: 34300275 INFO @ Tue, 14 Jul 2020 08:11:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:11:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:11:48: #1 tags after filtering in treatment: 34300275 INFO @ Tue, 14 Jul 2020 08:11:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:11:48: #1 finished! INFO @ Tue, 14 Jul 2020 08:11:48: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:11:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:11:50: #2 number of paired peaks: 20 WARNING @ Tue, 14 Jul 2020 08:11:50: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:11:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008768/SRX3008768.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling