Job ID = 6626588 SRX = SRX3008765 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:48 41940504 reads; of these: 41940504 (100.00%) were unpaired; of these: 611431 (1.46%) aligned 0 times 33843851 (80.69%) aligned exactly 1 time 7485222 (17.85%) aligned >1 times 98.54% overall alignment rate Time searching: 00:10:48 Overall time: 00:10:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7441575 / 41329073 = 0.1801 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:58:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:58:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:58:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:58:39: 1000000 INFO @ Tue, 14 Jul 2020 07:58:44: 2000000 INFO @ Tue, 14 Jul 2020 07:58:49: 3000000 INFO @ Tue, 14 Jul 2020 07:58:54: 4000000 INFO @ Tue, 14 Jul 2020 07:58:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:59:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:59:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:59:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:59:04: 6000000 INFO @ Tue, 14 Jul 2020 07:59:10: 1000000 INFO @ Tue, 14 Jul 2020 07:59:10: 7000000 INFO @ Tue, 14 Jul 2020 07:59:16: 2000000 INFO @ Tue, 14 Jul 2020 07:59:16: 8000000 INFO @ Tue, 14 Jul 2020 07:59:22: 3000000 INFO @ Tue, 14 Jul 2020 07:59:22: 9000000 INFO @ Tue, 14 Jul 2020 07:59:28: 10000000 INFO @ Tue, 14 Jul 2020 07:59:28: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:59:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:59:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:59:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:59:34: 11000000 INFO @ Tue, 14 Jul 2020 07:59:34: 5000000 INFO @ Tue, 14 Jul 2020 07:59:39: 1000000 INFO @ Tue, 14 Jul 2020 07:59:40: 12000000 INFO @ Tue, 14 Jul 2020 07:59:40: 6000000 INFO @ Tue, 14 Jul 2020 07:59:45: 2000000 INFO @ Tue, 14 Jul 2020 07:59:46: 13000000 INFO @ Tue, 14 Jul 2020 07:59:47: 7000000 INFO @ Tue, 14 Jul 2020 07:59:52: 3000000 INFO @ Tue, 14 Jul 2020 07:59:52: 14000000 INFO @ Tue, 14 Jul 2020 07:59:53: 8000000 INFO @ Tue, 14 Jul 2020 07:59:58: 4000000 INFO @ Tue, 14 Jul 2020 07:59:58: 15000000 INFO @ Tue, 14 Jul 2020 08:00:00: 9000000 INFO @ Tue, 14 Jul 2020 08:00:04: 5000000 INFO @ Tue, 14 Jul 2020 08:00:04: 16000000 INFO @ Tue, 14 Jul 2020 08:00:06: 10000000 INFO @ Tue, 14 Jul 2020 08:00:10: 6000000 INFO @ Tue, 14 Jul 2020 08:00:10: 17000000 INFO @ Tue, 14 Jul 2020 08:00:12: 11000000 INFO @ Tue, 14 Jul 2020 08:00:16: 7000000 INFO @ Tue, 14 Jul 2020 08:00:16: 18000000 INFO @ Tue, 14 Jul 2020 08:00:18: 12000000 INFO @ Tue, 14 Jul 2020 08:00:22: 8000000 INFO @ Tue, 14 Jul 2020 08:00:22: 19000000 INFO @ Tue, 14 Jul 2020 08:00:23: 13000000 INFO @ Tue, 14 Jul 2020 08:00:28: 9000000 INFO @ Tue, 14 Jul 2020 08:00:28: 20000000 INFO @ Tue, 14 Jul 2020 08:00:29: 14000000 INFO @ Tue, 14 Jul 2020 08:00:34: 21000000 INFO @ Tue, 14 Jul 2020 08:00:34: 10000000 INFO @ Tue, 14 Jul 2020 08:00:35: 15000000 INFO @ Tue, 14 Jul 2020 08:00:39: 22000000 INFO @ Tue, 14 Jul 2020 08:00:39: 11000000 INFO @ Tue, 14 Jul 2020 08:00:41: 16000000 INFO @ Tue, 14 Jul 2020 08:00:45: 23000000 INFO @ Tue, 14 Jul 2020 08:00:45: 12000000 INFO @ Tue, 14 Jul 2020 08:00:47: 17000000 INFO @ Tue, 14 Jul 2020 08:00:51: 24000000 INFO @ Tue, 14 Jul 2020 08:00:51: 13000000 INFO @ Tue, 14 Jul 2020 08:00:53: 18000000 INFO @ Tue, 14 Jul 2020 08:00:57: 25000000 INFO @ Tue, 14 Jul 2020 08:00:57: 14000000 INFO @ Tue, 14 Jul 2020 08:00:59: 19000000 INFO @ Tue, 14 Jul 2020 08:01:02: 26000000 INFO @ Tue, 14 Jul 2020 08:01:03: 15000000 INFO @ Tue, 14 Jul 2020 08:01:05: 20000000 INFO @ Tue, 14 Jul 2020 08:01:08: 27000000 INFO @ Tue, 14 Jul 2020 08:01:09: 16000000 INFO @ Tue, 14 Jul 2020 08:01:12: 21000000 INFO @ Tue, 14 Jul 2020 08:01:14: 28000000 INFO @ Tue, 14 Jul 2020 08:01:15: 17000000 INFO @ Tue, 14 Jul 2020 08:01:18: 22000000 INFO @ Tue, 14 Jul 2020 08:01:20: 29000000 INFO @ Tue, 14 Jul 2020 08:01:21: 18000000 INFO @ Tue, 14 Jul 2020 08:01:24: 23000000 INFO @ Tue, 14 Jul 2020 08:01:26: 30000000 INFO @ Tue, 14 Jul 2020 08:01:27: 19000000 INFO @ Tue, 14 Jul 2020 08:01:30: 24000000 INFO @ Tue, 14 Jul 2020 08:01:32: 31000000 INFO @ Tue, 14 Jul 2020 08:01:33: 20000000 INFO @ Tue, 14 Jul 2020 08:01:35: 25000000 INFO @ Tue, 14 Jul 2020 08:01:38: 32000000 INFO @ Tue, 14 Jul 2020 08:01:39: 21000000 INFO @ Tue, 14 Jul 2020 08:01:41: 26000000 INFO @ Tue, 14 Jul 2020 08:01:44: 33000000 INFO @ Tue, 14 Jul 2020 08:01:45: 22000000 INFO @ Tue, 14 Jul 2020 08:01:48: 27000000 INFO @ Tue, 14 Jul 2020 08:01:49: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:01:49: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:01:49: #1 total tags in treatment: 33887498 INFO @ Tue, 14 Jul 2020 08:01:49: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:01:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:01:50: #1 tags after filtering in treatment: 33887498 INFO @ Tue, 14 Jul 2020 08:01:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:01:50: #1 finished! INFO @ Tue, 14 Jul 2020 08:01:50: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:01:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:01:51: 23000000 INFO @ Tue, 14 Jul 2020 08:01:52: #2 number of paired peaks: 33 WARNING @ Tue, 14 Jul 2020 08:01:52: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:01:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.05_peaks.narrowPeak: No such file or directory BedGraph に変換しました。 BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:01:53: 28000000 INFO @ Tue, 14 Jul 2020 08:01:57: 24000000 INFO @ Tue, 14 Jul 2020 08:01:59: 29000000 INFO @ Tue, 14 Jul 2020 08:02:03: 25000000 INFO @ Tue, 14 Jul 2020 08:02:05: 30000000 INFO @ Tue, 14 Jul 2020 08:02:08: 26000000 INFO @ Tue, 14 Jul 2020 08:02:11: 31000000 INFO @ Tue, 14 Jul 2020 08:02:14: 27000000 INFO @ Tue, 14 Jul 2020 08:02:17: 32000000 INFO @ Tue, 14 Jul 2020 08:02:20: 28000000 INFO @ Tue, 14 Jul 2020 08:02:22: 33000000 INFO @ Tue, 14 Jul 2020 08:02:26: 29000000 INFO @ Tue, 14 Jul 2020 08:02:28: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:02:28: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:02:28: #1 total tags in treatment: 33887498 INFO @ Tue, 14 Jul 2020 08:02:28: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:02:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:02:28: #1 tags after filtering in treatment: 33887498 INFO @ Tue, 14 Jul 2020 08:02:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:02:28: #1 finished! INFO @ Tue, 14 Jul 2020 08:02:28: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:02:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:02:30: #2 number of paired peaks: 33 WARNING @ Tue, 14 Jul 2020 08:02:30: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:02:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:02:31: 30000000 INFO @ Tue, 14 Jul 2020 08:02:37: 31000000 INFO @ Tue, 14 Jul 2020 08:02:42: 32000000 INFO @ Tue, 14 Jul 2020 08:02:48: 33000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:02:53: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:02:53: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:02:53: #1 total tags in treatment: 33887498 INFO @ Tue, 14 Jul 2020 08:02:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:02:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:02:53: #1 tags after filtering in treatment: 33887498 INFO @ Tue, 14 Jul 2020 08:02:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:02:53: #1 finished! INFO @ Tue, 14 Jul 2020 08:02:53: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:02:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:02:56: #2 number of paired peaks: 33 WARNING @ Tue, 14 Jul 2020 08:02:56: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:02:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008765/SRX3008765.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling