Job ID = 6626590 SRX = SRX3008764 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:10 45176912 reads; of these: 45176912 (100.00%) were unpaired; of these: 2583939 (5.72%) aligned 0 times 35587966 (78.77%) aligned exactly 1 time 7005007 (15.51%) aligned >1 times 94.28% overall alignment rate Time searching: 00:10:10 Overall time: 00:10:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7350970 / 42592973 = 0.1726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:38: 1000000 INFO @ Tue, 14 Jul 2020 08:00:44: 2000000 INFO @ Tue, 14 Jul 2020 08:00:49: 3000000 INFO @ Tue, 14 Jul 2020 08:00:55: 4000000 INFO @ Tue, 14 Jul 2020 08:01:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:01:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:01:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:01:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:06: 6000000 INFO @ Tue, 14 Jul 2020 08:01:12: 7000000 INFO @ Tue, 14 Jul 2020 08:01:12: 1000000 INFO @ Tue, 14 Jul 2020 08:01:18: 8000000 INFO @ Tue, 14 Jul 2020 08:01:21: 2000000 INFO @ Tue, 14 Jul 2020 08:01:25: 9000000 INFO @ Tue, 14 Jul 2020 08:01:30: 3000000 INFO @ Tue, 14 Jul 2020 08:01:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:01:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:01:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:01:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:37: 11000000 INFO @ Tue, 14 Jul 2020 08:01:39: 4000000 INFO @ Tue, 14 Jul 2020 08:01:39: 1000000 INFO @ Tue, 14 Jul 2020 08:01:43: 12000000 INFO @ Tue, 14 Jul 2020 08:01:46: 2000000 INFO @ Tue, 14 Jul 2020 08:01:49: 5000000 INFO @ Tue, 14 Jul 2020 08:01:50: 13000000 INFO @ Tue, 14 Jul 2020 08:01:52: 3000000 INFO @ Tue, 14 Jul 2020 08:01:56: 14000000 INFO @ Tue, 14 Jul 2020 08:01:57: 6000000 INFO @ Tue, 14 Jul 2020 08:01:58: 4000000 INFO @ Tue, 14 Jul 2020 08:02:02: 15000000 INFO @ Tue, 14 Jul 2020 08:02:05: 5000000 INFO @ Tue, 14 Jul 2020 08:02:06: 7000000 INFO @ Tue, 14 Jul 2020 08:02:09: 16000000 INFO @ Tue, 14 Jul 2020 08:02:11: 6000000 INFO @ Tue, 14 Jul 2020 08:02:15: 17000000 INFO @ Tue, 14 Jul 2020 08:02:15: 8000000 INFO @ Tue, 14 Jul 2020 08:02:18: 7000000 INFO @ Tue, 14 Jul 2020 08:02:21: 18000000 INFO @ Tue, 14 Jul 2020 08:02:24: 9000000 INFO @ Tue, 14 Jul 2020 08:02:24: 8000000 INFO @ Tue, 14 Jul 2020 08:02:27: 19000000 INFO @ Tue, 14 Jul 2020 08:02:30: 9000000 INFO @ Tue, 14 Jul 2020 08:02:32: 10000000 INFO @ Tue, 14 Jul 2020 08:02:33: 20000000 INFO @ Tue, 14 Jul 2020 08:02:37: 10000000 INFO @ Tue, 14 Jul 2020 08:02:40: 21000000 INFO @ Tue, 14 Jul 2020 08:02:41: 11000000 INFO @ Tue, 14 Jul 2020 08:02:43: 11000000 INFO @ Tue, 14 Jul 2020 08:02:46: 22000000 INFO @ Tue, 14 Jul 2020 08:02:50: 12000000 INFO @ Tue, 14 Jul 2020 08:02:50: 12000000 INFO @ Tue, 14 Jul 2020 08:02:52: 23000000 INFO @ Tue, 14 Jul 2020 08:02:57: 13000000 INFO @ Tue, 14 Jul 2020 08:02:59: 24000000 INFO @ Tue, 14 Jul 2020 08:02:59: 13000000 INFO @ Tue, 14 Jul 2020 08:03:03: 14000000 INFO @ Tue, 14 Jul 2020 08:03:05: 25000000 INFO @ Tue, 14 Jul 2020 08:03:08: 14000000 INFO @ Tue, 14 Jul 2020 08:03:09: 15000000 INFO @ Tue, 14 Jul 2020 08:03:11: 26000000 INFO @ Tue, 14 Jul 2020 08:03:16: 16000000 INFO @ Tue, 14 Jul 2020 08:03:16: 15000000 INFO @ Tue, 14 Jul 2020 08:03:17: 27000000 INFO @ Tue, 14 Jul 2020 08:03:22: 17000000 INFO @ Tue, 14 Jul 2020 08:03:24: 28000000 INFO @ Tue, 14 Jul 2020 08:03:25: 16000000 INFO @ Tue, 14 Jul 2020 08:03:28: 18000000 INFO @ Tue, 14 Jul 2020 08:03:30: 29000000 INFO @ Tue, 14 Jul 2020 08:03:34: 17000000 INFO @ Tue, 14 Jul 2020 08:03:34: 19000000 INFO @ Tue, 14 Jul 2020 08:03:36: 30000000 INFO @ Tue, 14 Jul 2020 08:03:41: 20000000 INFO @ Tue, 14 Jul 2020 08:03:42: 18000000 INFO @ Tue, 14 Jul 2020 08:03:43: 31000000 INFO @ Tue, 14 Jul 2020 08:03:47: 21000000 INFO @ Tue, 14 Jul 2020 08:03:49: 32000000 INFO @ Tue, 14 Jul 2020 08:03:50: 19000000 INFO @ Tue, 14 Jul 2020 08:03:54: 22000000 INFO @ Tue, 14 Jul 2020 08:03:55: 33000000 INFO @ Tue, 14 Jul 2020 08:03:57: 20000000 INFO @ Tue, 14 Jul 2020 08:04:00: 23000000 INFO @ Tue, 14 Jul 2020 08:04:01: 34000000 INFO @ Tue, 14 Jul 2020 08:04:04: 21000000 INFO @ Tue, 14 Jul 2020 08:04:06: 24000000 INFO @ Tue, 14 Jul 2020 08:04:08: 35000000 INFO @ Tue, 14 Jul 2020 08:04:09: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:04:09: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:04:09: #1 total tags in treatment: 35242003 INFO @ Tue, 14 Jul 2020 08:04:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:04:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:04:10: #1 tags after filtering in treatment: 35242003 INFO @ Tue, 14 Jul 2020 08:04:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:04:10: #1 finished! INFO @ Tue, 14 Jul 2020 08:04:10: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:04:10: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:04:11: 22000000 INFO @ Tue, 14 Jul 2020 08:04:12: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 08:04:12: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:04:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:04:13: 25000000 INFO @ Tue, 14 Jul 2020 08:04:18: 23000000 INFO @ Tue, 14 Jul 2020 08:04:19: 26000000 INFO @ Tue, 14 Jul 2020 08:04:25: 27000000 INFO @ Tue, 14 Jul 2020 08:04:25: 24000000 INFO @ Tue, 14 Jul 2020 08:04:31: 28000000 INFO @ Tue, 14 Jul 2020 08:04:32: 25000000 INFO @ Tue, 14 Jul 2020 08:04:37: 29000000 INFO @ Tue, 14 Jul 2020 08:04:39: 26000000 INFO @ Tue, 14 Jul 2020 08:04:43: 30000000 INFO @ Tue, 14 Jul 2020 08:04:46: 27000000 INFO @ Tue, 14 Jul 2020 08:04:50: 31000000 INFO @ Tue, 14 Jul 2020 08:04:52: 28000000 INFO @ Tue, 14 Jul 2020 08:04:56: 32000000 INFO @ Tue, 14 Jul 2020 08:04:59: 29000000 INFO @ Tue, 14 Jul 2020 08:05:02: 33000000 INFO @ Tue, 14 Jul 2020 08:05:06: 30000000 INFO @ Tue, 14 Jul 2020 08:05:08: 34000000 INFO @ Tue, 14 Jul 2020 08:05:13: 31000000 INFO @ Tue, 14 Jul 2020 08:05:14: 35000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:05:16: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:05:16: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:05:16: #1 total tags in treatment: 35242003 INFO @ Tue, 14 Jul 2020 08:05:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:16: #1 tags after filtering in treatment: 35242003 INFO @ Tue, 14 Jul 2020 08:05:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:16: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:16: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:19: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 08:05:19: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:05:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:05:19: 32000000 INFO @ Tue, 14 Jul 2020 08:05:26: 33000000 INFO @ Tue, 14 Jul 2020 08:05:32: 34000000 INFO @ Tue, 14 Jul 2020 08:05:39: 35000000 INFO @ Tue, 14 Jul 2020 08:05:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:05:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:05:41: #1 total tags in treatment: 35242003 INFO @ Tue, 14 Jul 2020 08:05:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:05:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:05:41: #1 tags after filtering in treatment: 35242003 INFO @ Tue, 14 Jul 2020 08:05:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:05:41: #1 finished! INFO @ Tue, 14 Jul 2020 08:05:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:05:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:05:43: #2 number of paired peaks: 39 WARNING @ Tue, 14 Jul 2020 08:05:43: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:05:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008764/SRX3008764.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling