Job ID = 6626582 SRX = SRX3008763 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:50 39763228 reads; of these: 39763228 (100.00%) were unpaired; of these: 1219710 (3.07%) aligned 0 times 32311688 (81.26%) aligned exactly 1 time 6231830 (15.67%) aligned >1 times 96.93% overall alignment rate Time searching: 00:08:50 Overall time: 00:08:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6495051 / 38543518 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:54:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:54:39: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:54:39: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:54:45: 1000000 INFO @ Tue, 14 Jul 2020 07:54:52: 2000000 INFO @ Tue, 14 Jul 2020 07:54:58: 3000000 INFO @ Tue, 14 Jul 2020 07:55:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:55:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:55:09: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:55:09: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:55:11: 5000000 INFO @ Tue, 14 Jul 2020 07:55:15: 1000000 INFO @ Tue, 14 Jul 2020 07:55:18: 6000000 INFO @ Tue, 14 Jul 2020 07:55:20: 2000000 INFO @ Tue, 14 Jul 2020 07:55:24: 7000000 INFO @ Tue, 14 Jul 2020 07:55:26: 3000000 INFO @ Tue, 14 Jul 2020 07:55:31: 8000000 INFO @ Tue, 14 Jul 2020 07:55:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:55:37: 5000000 INFO @ Tue, 14 Jul 2020 07:55:38: 9000000 INFO @ Tue, 14 Jul 2020 07:55:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:55:39: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:55:39: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:55:43: 6000000 INFO @ Tue, 14 Jul 2020 07:55:44: 10000000 INFO @ Tue, 14 Jul 2020 07:55:45: 1000000 INFO @ Tue, 14 Jul 2020 07:55:48: 7000000 INFO @ Tue, 14 Jul 2020 07:55:51: 11000000 INFO @ Tue, 14 Jul 2020 07:55:52: 2000000 INFO @ Tue, 14 Jul 2020 07:55:54: 8000000 INFO @ Tue, 14 Jul 2020 07:55:58: 12000000 INFO @ Tue, 14 Jul 2020 07:55:58: 3000000 INFO @ Tue, 14 Jul 2020 07:56:00: 9000000 INFO @ Tue, 14 Jul 2020 07:56:04: 13000000 INFO @ Tue, 14 Jul 2020 07:56:05: 4000000 INFO @ Tue, 14 Jul 2020 07:56:05: 10000000 INFO @ Tue, 14 Jul 2020 07:56:10: 14000000 INFO @ Tue, 14 Jul 2020 07:56:11: 11000000 INFO @ Tue, 14 Jul 2020 07:56:11: 5000000 INFO @ Tue, 14 Jul 2020 07:56:17: 12000000 INFO @ Tue, 14 Jul 2020 07:56:17: 15000000 INFO @ Tue, 14 Jul 2020 07:56:18: 6000000 INFO @ Tue, 14 Jul 2020 07:56:22: 13000000 INFO @ Tue, 14 Jul 2020 07:56:23: 16000000 INFO @ Tue, 14 Jul 2020 07:56:24: 7000000 INFO @ Tue, 14 Jul 2020 07:56:28: 14000000 INFO @ Tue, 14 Jul 2020 07:56:30: 17000000 INFO @ Tue, 14 Jul 2020 07:56:30: 8000000 INFO @ Tue, 14 Jul 2020 07:56:34: 15000000 INFO @ Tue, 14 Jul 2020 07:56:36: 18000000 INFO @ Tue, 14 Jul 2020 07:56:36: 9000000 INFO @ Tue, 14 Jul 2020 07:56:39: 16000000 INFO @ Tue, 14 Jul 2020 07:56:42: 19000000 INFO @ Tue, 14 Jul 2020 07:56:43: 10000000 INFO @ Tue, 14 Jul 2020 07:56:45: 17000000 INFO @ Tue, 14 Jul 2020 07:56:48: 20000000 INFO @ Tue, 14 Jul 2020 07:56:49: 11000000 INFO @ Tue, 14 Jul 2020 07:56:50: 18000000 INFO @ Tue, 14 Jul 2020 07:56:55: 21000000 INFO @ Tue, 14 Jul 2020 07:56:55: 12000000 INFO @ Tue, 14 Jul 2020 07:56:56: 19000000 INFO @ Tue, 14 Jul 2020 07:57:01: 22000000 INFO @ Tue, 14 Jul 2020 07:57:01: 13000000 INFO @ Tue, 14 Jul 2020 07:57:01: 20000000 INFO @ Tue, 14 Jul 2020 07:57:07: 23000000 INFO @ Tue, 14 Jul 2020 07:57:07: 21000000 INFO @ Tue, 14 Jul 2020 07:57:08: 14000000 INFO @ Tue, 14 Jul 2020 07:57:13: 22000000 INFO @ Tue, 14 Jul 2020 07:57:13: 24000000 INFO @ Tue, 14 Jul 2020 07:57:14: 15000000 INFO @ Tue, 14 Jul 2020 07:57:18: 23000000 INFO @ Tue, 14 Jul 2020 07:57:19: 25000000 INFO @ Tue, 14 Jul 2020 07:57:20: 16000000 INFO @ Tue, 14 Jul 2020 07:57:24: 24000000 INFO @ Tue, 14 Jul 2020 07:57:26: 26000000 INFO @ Tue, 14 Jul 2020 07:57:26: 17000000 INFO @ Tue, 14 Jul 2020 07:57:29: 25000000 INFO @ Tue, 14 Jul 2020 07:57:32: 27000000 INFO @ Tue, 14 Jul 2020 07:57:33: 18000000 INFO @ Tue, 14 Jul 2020 07:57:35: 26000000 INFO @ Tue, 14 Jul 2020 07:57:38: 28000000 INFO @ Tue, 14 Jul 2020 07:57:39: 19000000 INFO @ Tue, 14 Jul 2020 07:57:40: 27000000 INFO @ Tue, 14 Jul 2020 07:57:44: 29000000 INFO @ Tue, 14 Jul 2020 07:57:45: 20000000 INFO @ Tue, 14 Jul 2020 07:57:46: 28000000 INFO @ Tue, 14 Jul 2020 07:57:50: 30000000 INFO @ Tue, 14 Jul 2020 07:57:51: 21000000 INFO @ Tue, 14 Jul 2020 07:57:51: 29000000 INFO @ Tue, 14 Jul 2020 07:57:56: 31000000 INFO @ Tue, 14 Jul 2020 07:57:57: 30000000 INFO @ Tue, 14 Jul 2020 07:57:57: 22000000 INFO @ Tue, 14 Jul 2020 07:58:02: 31000000 INFO @ Tue, 14 Jul 2020 07:58:03: 32000000 INFO @ Tue, 14 Jul 2020 07:58:03: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:58:03: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:58:03: #1 total tags in treatment: 32048467 INFO @ Tue, 14 Jul 2020 07:58:03: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:58:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:58:03: 23000000 INFO @ Tue, 14 Jul 2020 07:58:04: #1 tags after filtering in treatment: 32048467 INFO @ Tue, 14 Jul 2020 07:58:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:58:04: #1 finished! INFO @ Tue, 14 Jul 2020 07:58:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:58:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:58:05: #2 number of paired peaks: 72 WARNING @ Tue, 14 Jul 2020 07:58:05: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:58:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:58:08: 32000000 INFO @ Tue, 14 Jul 2020 07:58:08: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:58:08: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:58:08: #1 total tags in treatment: 32048467 INFO @ Tue, 14 Jul 2020 07:58:08: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:58:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:58:09: #1 tags after filtering in treatment: 32048467 INFO @ Tue, 14 Jul 2020 07:58:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:58:09: #1 finished! INFO @ Tue, 14 Jul 2020 07:58:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:58:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:58:09: 24000000 INFO @ Tue, 14 Jul 2020 07:58:11: #2 number of paired peaks: 72 WARNING @ Tue, 14 Jul 2020 07:58:11: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:58:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:58:15: 25000000 INFO @ Tue, 14 Jul 2020 07:58:21: 26000000 INFO @ Tue, 14 Jul 2020 07:58:27: 27000000 INFO @ Tue, 14 Jul 2020 07:58:33: 28000000 INFO @ Tue, 14 Jul 2020 07:58:39: 29000000 INFO @ Tue, 14 Jul 2020 07:58:44: 30000000 INFO @ Tue, 14 Jul 2020 07:58:50: 31000000 INFO @ Tue, 14 Jul 2020 07:58:56: 32000000 INFO @ Tue, 14 Jul 2020 07:58:56: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:58:56: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:58:56: #1 total tags in treatment: 32048467 INFO @ Tue, 14 Jul 2020 07:58:56: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:58:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:58:57: #1 tags after filtering in treatment: 32048467 INFO @ Tue, 14 Jul 2020 07:58:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:58:57: #1 finished! INFO @ Tue, 14 Jul 2020 07:58:57: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:58:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:58:59: #2 number of paired peaks: 72 WARNING @ Tue, 14 Jul 2020 07:58:59: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:58:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008763/SRX3008763.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。