Job ID = 6626592 SRX = SRX3008762 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:12 43398663 reads; of these: 43398663 (100.00%) were unpaired; of these: 699445 (1.61%) aligned 0 times 35755402 (82.39%) aligned exactly 1 time 6943816 (16.00%) aligned >1 times 98.39% overall alignment rate Time searching: 00:09:12 Overall time: 00:09:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7319709 / 42699218 = 0.1714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:21: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:21: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:26: 1000000 INFO @ Tue, 14 Jul 2020 08:00:32: 2000000 INFO @ Tue, 14 Jul 2020 08:00:37: 3000000 INFO @ Tue, 14 Jul 2020 08:00:43: 4000000 INFO @ Tue, 14 Jul 2020 08:00:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:00:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:00:51: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:00:51: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:00:54: 6000000 INFO @ Tue, 14 Jul 2020 08:00:56: 1000000 INFO @ Tue, 14 Jul 2020 08:01:00: 7000000 INFO @ Tue, 14 Jul 2020 08:01:02: 2000000 INFO @ Tue, 14 Jul 2020 08:01:05: 8000000 INFO @ Tue, 14 Jul 2020 08:01:08: 3000000 INFO @ Tue, 14 Jul 2020 08:01:11: 9000000 INFO @ Tue, 14 Jul 2020 08:01:13: 4000000 INFO @ Tue, 14 Jul 2020 08:01:17: 10000000 INFO @ Tue, 14 Jul 2020 08:01:19: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:01:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:01:21: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:01:21: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:01:22: 11000000 INFO @ Tue, 14 Jul 2020 08:01:24: 6000000 INFO @ Tue, 14 Jul 2020 08:01:27: 1000000 INFO @ Tue, 14 Jul 2020 08:01:28: 12000000 INFO @ Tue, 14 Jul 2020 08:01:30: 7000000 INFO @ Tue, 14 Jul 2020 08:01:33: 2000000 INFO @ Tue, 14 Jul 2020 08:01:34: 13000000 INFO @ Tue, 14 Jul 2020 08:01:36: 8000000 INFO @ Tue, 14 Jul 2020 08:01:38: 3000000 INFO @ Tue, 14 Jul 2020 08:01:39: 14000000 INFO @ Tue, 14 Jul 2020 08:01:41: 9000000 INFO @ Tue, 14 Jul 2020 08:01:44: 4000000 INFO @ Tue, 14 Jul 2020 08:01:45: 15000000 INFO @ Tue, 14 Jul 2020 08:01:47: 10000000 INFO @ Tue, 14 Jul 2020 08:01:50: 5000000 INFO @ Tue, 14 Jul 2020 08:01:51: 16000000 INFO @ Tue, 14 Jul 2020 08:01:53: 11000000 INFO @ Tue, 14 Jul 2020 08:01:55: 6000000 INFO @ Tue, 14 Jul 2020 08:01:56: 17000000 INFO @ Tue, 14 Jul 2020 08:01:58: 12000000 INFO @ Tue, 14 Jul 2020 08:02:01: 7000000 INFO @ Tue, 14 Jul 2020 08:02:02: 18000000 INFO @ Tue, 14 Jul 2020 08:02:04: 13000000 INFO @ Tue, 14 Jul 2020 08:02:07: 8000000 INFO @ Tue, 14 Jul 2020 08:02:08: 19000000 INFO @ Tue, 14 Jul 2020 08:02:10: 14000000 INFO @ Tue, 14 Jul 2020 08:02:12: 9000000 INFO @ Tue, 14 Jul 2020 08:02:13: 20000000 INFO @ Tue, 14 Jul 2020 08:02:15: 15000000 INFO @ Tue, 14 Jul 2020 08:02:18: 10000000 INFO @ Tue, 14 Jul 2020 08:02:19: 21000000 INFO @ Tue, 14 Jul 2020 08:02:21: 16000000 INFO @ Tue, 14 Jul 2020 08:02:24: 11000000 INFO @ Tue, 14 Jul 2020 08:02:25: 22000000 INFO @ Tue, 14 Jul 2020 08:02:27: 17000000 INFO @ Tue, 14 Jul 2020 08:02:29: 12000000 INFO @ Tue, 14 Jul 2020 08:02:31: 23000000 INFO @ Tue, 14 Jul 2020 08:02:32: 18000000 INFO @ Tue, 14 Jul 2020 08:02:35: 13000000 INFO @ Tue, 14 Jul 2020 08:02:36: 24000000 INFO @ Tue, 14 Jul 2020 08:02:38: 19000000 INFO @ Tue, 14 Jul 2020 08:02:41: 14000000 INFO @ Tue, 14 Jul 2020 08:02:42: 25000000 INFO @ Tue, 14 Jul 2020 08:02:44: 20000000 INFO @ Tue, 14 Jul 2020 08:02:46: 15000000 INFO @ Tue, 14 Jul 2020 08:02:48: 26000000 INFO @ Tue, 14 Jul 2020 08:02:49: 21000000 INFO @ Tue, 14 Jul 2020 08:02:52: 16000000 INFO @ Tue, 14 Jul 2020 08:02:53: 27000000 INFO @ Tue, 14 Jul 2020 08:02:55: 22000000 INFO @ Tue, 14 Jul 2020 08:02:58: 17000000 INFO @ Tue, 14 Jul 2020 08:02:59: 28000000 INFO @ Tue, 14 Jul 2020 08:03:01: 23000000 INFO @ Tue, 14 Jul 2020 08:03:03: 18000000 INFO @ Tue, 14 Jul 2020 08:03:05: 29000000 INFO @ Tue, 14 Jul 2020 08:03:06: 24000000 INFO @ Tue, 14 Jul 2020 08:03:09: 19000000 INFO @ Tue, 14 Jul 2020 08:03:10: 30000000 INFO @ Tue, 14 Jul 2020 08:03:12: 25000000 INFO @ Tue, 14 Jul 2020 08:03:15: 20000000 INFO @ Tue, 14 Jul 2020 08:03:16: 31000000 INFO @ Tue, 14 Jul 2020 08:03:18: 26000000 INFO @ Tue, 14 Jul 2020 08:03:20: 21000000 INFO @ Tue, 14 Jul 2020 08:03:22: 32000000 INFO @ Tue, 14 Jul 2020 08:03:23: 27000000 INFO @ Tue, 14 Jul 2020 08:03:26: 22000000 INFO @ Tue, 14 Jul 2020 08:03:27: 33000000 INFO @ Tue, 14 Jul 2020 08:03:29: 28000000 INFO @ Tue, 14 Jul 2020 08:03:32: 23000000 INFO @ Tue, 14 Jul 2020 08:03:33: 34000000 INFO @ Tue, 14 Jul 2020 08:03:34: 29000000 INFO @ Tue, 14 Jul 2020 08:03:38: 24000000 INFO @ Tue, 14 Jul 2020 08:03:39: 35000000 INFO @ Tue, 14 Jul 2020 08:03:40: 30000000 INFO @ Tue, 14 Jul 2020 08:03:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:03:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:03:41: #1 total tags in treatment: 35379509 INFO @ Tue, 14 Jul 2020 08:03:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:03:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:03:42: #1 tags after filtering in treatment: 35379509 INFO @ Tue, 14 Jul 2020 08:03:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:03:42: #1 finished! INFO @ Tue, 14 Jul 2020 08:03:42: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:03:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:03:43: 25000000 INFO @ Tue, 14 Jul 2020 08:03:44: #2 number of paired peaks: 37 WARNING @ Tue, 14 Jul 2020 08:03:44: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:03:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:03:46: 31000000 INFO @ Tue, 14 Jul 2020 08:03:49: 26000000 INFO @ Tue, 14 Jul 2020 08:03:51: 32000000 INFO @ Tue, 14 Jul 2020 08:03:54: 27000000 INFO @ Tue, 14 Jul 2020 08:03:57: 33000000 INFO @ Tue, 14 Jul 2020 08:04:00: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:04:02: 34000000 INFO @ Tue, 14 Jul 2020 08:04:06: 29000000 INFO @ Tue, 14 Jul 2020 08:04:08: 35000000 INFO @ Tue, 14 Jul 2020 08:04:10: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:04:10: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:04:10: #1 total tags in treatment: 35379509 INFO @ Tue, 14 Jul 2020 08:04:10: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:04:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:04:11: #1 tags after filtering in treatment: 35379509 INFO @ Tue, 14 Jul 2020 08:04:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:04:11: #1 finished! INFO @ Tue, 14 Jul 2020 08:04:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:04:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:04:11: 30000000 INFO @ Tue, 14 Jul 2020 08:04:13: #2 number of paired peaks: 37 WARNING @ Tue, 14 Jul 2020 08:04:13: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:04:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:04:17: 31000000 INFO @ Tue, 14 Jul 2020 08:04:22: 32000000 INFO @ Tue, 14 Jul 2020 08:04:28: 33000000 INFO @ Tue, 14 Jul 2020 08:04:34: 34000000 INFO @ Tue, 14 Jul 2020 08:04:39: 35000000 INFO @ Tue, 14 Jul 2020 08:04:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:04:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:04:41: #1 total tags in treatment: 35379509 INFO @ Tue, 14 Jul 2020 08:04:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:04:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:04:42: #1 tags after filtering in treatment: 35379509 INFO @ Tue, 14 Jul 2020 08:04:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:04:42: #1 finished! INFO @ Tue, 14 Jul 2020 08:04:42: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:04:44: #2 number of paired peaks: 37 WARNING @ Tue, 14 Jul 2020 08:04:44: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:04:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008762/SRX3008762.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。