Job ID = 6626597 SRX = SRX3008761 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:03 46495373 reads; of these: 46495373 (100.00%) were unpaired; of these: 7328243 (15.76%) aligned 0 times 32700945 (70.33%) aligned exactly 1 time 6466185 (13.91%) aligned >1 times 84.24% overall alignment rate Time searching: 00:11:03 Overall time: 00:11:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6572454 / 39167130 = 0.1678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:03:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:03:36: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:03:36: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:03:43: 1000000 INFO @ Tue, 14 Jul 2020 08:03:49: 2000000 INFO @ Tue, 14 Jul 2020 08:03:55: 3000000 INFO @ Tue, 14 Jul 2020 08:04:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:04:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:04:06: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:04:06: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:04:07: 5000000 INFO @ Tue, 14 Jul 2020 08:04:13: 1000000 INFO @ Tue, 14 Jul 2020 08:04:14: 6000000 INFO @ Tue, 14 Jul 2020 08:04:20: 2000000 INFO @ Tue, 14 Jul 2020 08:04:21: 7000000 INFO @ Tue, 14 Jul 2020 08:04:26: 3000000 INFO @ Tue, 14 Jul 2020 08:04:27: 8000000 INFO @ Tue, 14 Jul 2020 08:04:32: 4000000 INFO @ Tue, 14 Jul 2020 08:04:33: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:04:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:04:36: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:04:36: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:04:38: 5000000 INFO @ Tue, 14 Jul 2020 08:04:39: 10000000 INFO @ Tue, 14 Jul 2020 08:04:43: 1000000 INFO @ Tue, 14 Jul 2020 08:04:44: 6000000 INFO @ Tue, 14 Jul 2020 08:04:46: 11000000 INFO @ Tue, 14 Jul 2020 08:04:49: 2000000 INFO @ Tue, 14 Jul 2020 08:04:51: 7000000 INFO @ Tue, 14 Jul 2020 08:04:53: 12000000 INFO @ Tue, 14 Jul 2020 08:04:56: 3000000 INFO @ Tue, 14 Jul 2020 08:04:57: 8000000 INFO @ Tue, 14 Jul 2020 08:05:00: 13000000 INFO @ Tue, 14 Jul 2020 08:05:03: 4000000 INFO @ Tue, 14 Jul 2020 08:05:04: 9000000 INFO @ Tue, 14 Jul 2020 08:05:07: 14000000 INFO @ Tue, 14 Jul 2020 08:05:09: 5000000 INFO @ Tue, 14 Jul 2020 08:05:11: 10000000 INFO @ Tue, 14 Jul 2020 08:05:13: 15000000 INFO @ Tue, 14 Jul 2020 08:05:16: 6000000 INFO @ Tue, 14 Jul 2020 08:05:18: 11000000 INFO @ Tue, 14 Jul 2020 08:05:20: 16000000 INFO @ Tue, 14 Jul 2020 08:05:23: 7000000 INFO @ Tue, 14 Jul 2020 08:05:24: 12000000 INFO @ Tue, 14 Jul 2020 08:05:26: 17000000 INFO @ Tue, 14 Jul 2020 08:05:30: 8000000 INFO @ Tue, 14 Jul 2020 08:05:31: 13000000 INFO @ Tue, 14 Jul 2020 08:05:33: 18000000 INFO @ Tue, 14 Jul 2020 08:05:37: 9000000 INFO @ Tue, 14 Jul 2020 08:05:38: 14000000 INFO @ Tue, 14 Jul 2020 08:05:39: 19000000 INFO @ Tue, 14 Jul 2020 08:05:43: 10000000 INFO @ Tue, 14 Jul 2020 08:05:45: 15000000 INFO @ Tue, 14 Jul 2020 08:05:46: 20000000 INFO @ Tue, 14 Jul 2020 08:05:50: 11000000 INFO @ Tue, 14 Jul 2020 08:05:51: 16000000 INFO @ Tue, 14 Jul 2020 08:05:52: 21000000 INFO @ Tue, 14 Jul 2020 08:05:57: 12000000 INFO @ Tue, 14 Jul 2020 08:05:58: 17000000 INFO @ Tue, 14 Jul 2020 08:05:59: 22000000 INFO @ Tue, 14 Jul 2020 08:06:03: 13000000 INFO @ Tue, 14 Jul 2020 08:06:04: 18000000 INFO @ Tue, 14 Jul 2020 08:06:05: 23000000 INFO @ Tue, 14 Jul 2020 08:06:10: 14000000 INFO @ Tue, 14 Jul 2020 08:06:11: 19000000 INFO @ Tue, 14 Jul 2020 08:06:11: 24000000 INFO @ Tue, 14 Jul 2020 08:06:17: 15000000 INFO @ Tue, 14 Jul 2020 08:06:18: 20000000 INFO @ Tue, 14 Jul 2020 08:06:18: 25000000 INFO @ Tue, 14 Jul 2020 08:06:23: 16000000 INFO @ Tue, 14 Jul 2020 08:06:24: 21000000 INFO @ Tue, 14 Jul 2020 08:06:24: 26000000 INFO @ Tue, 14 Jul 2020 08:06:30: 17000000 INFO @ Tue, 14 Jul 2020 08:06:31: 27000000 INFO @ Tue, 14 Jul 2020 08:06:31: 22000000 INFO @ Tue, 14 Jul 2020 08:06:37: 18000000 INFO @ Tue, 14 Jul 2020 08:06:37: 28000000 INFO @ Tue, 14 Jul 2020 08:06:38: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:06:44: 19000000 INFO @ Tue, 14 Jul 2020 08:06:44: 29000000 INFO @ Tue, 14 Jul 2020 08:06:45: 24000000 INFO @ Tue, 14 Jul 2020 08:06:51: 20000000 INFO @ Tue, 14 Jul 2020 08:06:51: 30000000 INFO @ Tue, 14 Jul 2020 08:06:52: 25000000 INFO @ Tue, 14 Jul 2020 08:06:58: 31000000 INFO @ Tue, 14 Jul 2020 08:06:58: 21000000 INFO @ Tue, 14 Jul 2020 08:06:59: 26000000 INFO @ Tue, 14 Jul 2020 08:07:04: 32000000 INFO @ Tue, 14 Jul 2020 08:07:05: 22000000 INFO @ Tue, 14 Jul 2020 08:07:06: 27000000 INFO @ Tue, 14 Jul 2020 08:07:09: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:07:09: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:07:09: #1 total tags in treatment: 32594676 INFO @ Tue, 14 Jul 2020 08:07:09: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:07:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:07:09: #1 tags after filtering in treatment: 32594676 INFO @ Tue, 14 Jul 2020 08:07:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:07:09: #1 finished! INFO @ Tue, 14 Jul 2020 08:07:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:07:11: #2 number of paired peaks: 64 WARNING @ Tue, 14 Jul 2020 08:07:11: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:07:12: 23000000 INFO @ Tue, 14 Jul 2020 08:07:13: 28000000 INFO @ Tue, 14 Jul 2020 08:07:19: 24000000 INFO @ Tue, 14 Jul 2020 08:07:19: 29000000 INFO @ Tue, 14 Jul 2020 08:07:25: 25000000 INFO @ Tue, 14 Jul 2020 08:07:26: 30000000 INFO @ Tue, 14 Jul 2020 08:07:32: 26000000 INFO @ Tue, 14 Jul 2020 08:07:32: 31000000 INFO @ Tue, 14 Jul 2020 08:07:38: 27000000 INFO @ Tue, 14 Jul 2020 08:07:39: 32000000 INFO @ Tue, 14 Jul 2020 08:07:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:07:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:07:43: #1 total tags in treatment: 32594676 INFO @ Tue, 14 Jul 2020 08:07:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:07:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:07:44: #1 tags after filtering in treatment: 32594676 INFO @ Tue, 14 Jul 2020 08:07:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:07:44: #1 finished! INFO @ Tue, 14 Jul 2020 08:07:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:07:44: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:07:45: 28000000 INFO @ Tue, 14 Jul 2020 08:07:45: #2 number of paired peaks: 64 WARNING @ Tue, 14 Jul 2020 08:07:45: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:07:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:07:51: 29000000 INFO @ Tue, 14 Jul 2020 08:07:57: 30000000 INFO @ Tue, 14 Jul 2020 08:08:03: 31000000 INFO @ Tue, 14 Jul 2020 08:08:09: 32000000 INFO @ Tue, 14 Jul 2020 08:08:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 08:08:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 08:08:13: #1 total tags in treatment: 32594676 INFO @ Tue, 14 Jul 2020 08:08:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:08:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:08:14: #1 tags after filtering in treatment: 32594676 INFO @ Tue, 14 Jul 2020 08:08:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 08:08:14: #1 finished! INFO @ Tue, 14 Jul 2020 08:08:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:08:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:08:16: #2 number of paired peaks: 64 WARNING @ Tue, 14 Jul 2020 08:08:16: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:08:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX3008761/SRX3008761.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling