Job ID = 6367210 SRX = SRX2985930 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:17:16 prefetch.2.10.7: 1) Downloading 'SRR5807080'... 2020-06-15T23:17:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:19:23 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:19:23 prefetch.2.10.7: 1) 'SRR5807080' was downloaded successfully 2020-06-15T23:19:23 prefetch.2.10.7: 'SRR5807080' has 0 unresolved dependencies Read 53040099 spots for SRR5807080/SRR5807080.sra Written 53040099 spots for SRR5807080/SRR5807080.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:49 53040099 reads; of these: 53040099 (100.00%) were unpaired; of these: 1336882 (2.52%) aligned 0 times 39802826 (75.04%) aligned exactly 1 time 11900391 (22.44%) aligned >1 times 97.48% overall alignment rate Time searching: 00:12:49 Overall time: 00:12:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 17657659 / 51703217 = 0.3415 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:43:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:43:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:43:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:43:12: 1000000 INFO @ Tue, 16 Jun 2020 08:43:17: 2000000 INFO @ Tue, 16 Jun 2020 08:43:23: 3000000 INFO @ Tue, 16 Jun 2020 08:43:28: 4000000 INFO @ Tue, 16 Jun 2020 08:43:33: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:43:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:43:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:43:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:43:39: 6000000 INFO @ Tue, 16 Jun 2020 08:43:42: 1000000 INFO @ Tue, 16 Jun 2020 08:43:44: 7000000 INFO @ Tue, 16 Jun 2020 08:43:48: 2000000 INFO @ Tue, 16 Jun 2020 08:43:50: 8000000 INFO @ Tue, 16 Jun 2020 08:43:54: 3000000 INFO @ Tue, 16 Jun 2020 08:43:56: 9000000 INFO @ Tue, 16 Jun 2020 08:43:59: 4000000 INFO @ Tue, 16 Jun 2020 08:44:02: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:44:05: 5000000 INFO @ Tue, 16 Jun 2020 08:44:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:44:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:44:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:44:08: 11000000 INFO @ Tue, 16 Jun 2020 08:44:11: 6000000 INFO @ Tue, 16 Jun 2020 08:44:12: 1000000 INFO @ Tue, 16 Jun 2020 08:44:14: 12000000 INFO @ Tue, 16 Jun 2020 08:44:17: 7000000 INFO @ Tue, 16 Jun 2020 08:44:18: 2000000 INFO @ Tue, 16 Jun 2020 08:44:20: 13000000 INFO @ Tue, 16 Jun 2020 08:44:23: 8000000 INFO @ Tue, 16 Jun 2020 08:44:24: 3000000 INFO @ Tue, 16 Jun 2020 08:44:26: 14000000 INFO @ Tue, 16 Jun 2020 08:44:28: 9000000 INFO @ Tue, 16 Jun 2020 08:44:30: 4000000 INFO @ Tue, 16 Jun 2020 08:44:32: 15000000 INFO @ Tue, 16 Jun 2020 08:44:34: 10000000 INFO @ Tue, 16 Jun 2020 08:44:36: 5000000 INFO @ Tue, 16 Jun 2020 08:44:38: 16000000 INFO @ Tue, 16 Jun 2020 08:44:40: 11000000 INFO @ Tue, 16 Jun 2020 08:44:42: 6000000 INFO @ Tue, 16 Jun 2020 08:44:44: 17000000 INFO @ Tue, 16 Jun 2020 08:44:46: 12000000 INFO @ Tue, 16 Jun 2020 08:44:48: 7000000 INFO @ Tue, 16 Jun 2020 08:44:49: 18000000 INFO @ Tue, 16 Jun 2020 08:44:52: 13000000 INFO @ Tue, 16 Jun 2020 08:44:54: 8000000 INFO @ Tue, 16 Jun 2020 08:44:55: 19000000 INFO @ Tue, 16 Jun 2020 08:44:58: 14000000 INFO @ Tue, 16 Jun 2020 08:45:00: 9000000 INFO @ Tue, 16 Jun 2020 08:45:01: 20000000 INFO @ Tue, 16 Jun 2020 08:45:04: 15000000 INFO @ Tue, 16 Jun 2020 08:45:05: 10000000 INFO @ Tue, 16 Jun 2020 08:45:07: 21000000 INFO @ Tue, 16 Jun 2020 08:45:09: 16000000 INFO @ Tue, 16 Jun 2020 08:45:11: 11000000 INFO @ Tue, 16 Jun 2020 08:45:13: 22000000 INFO @ Tue, 16 Jun 2020 08:45:15: 17000000 INFO @ Tue, 16 Jun 2020 08:45:17: 12000000 INFO @ Tue, 16 Jun 2020 08:45:18: 23000000 INFO @ Tue, 16 Jun 2020 08:45:21: 18000000 INFO @ Tue, 16 Jun 2020 08:45:23: 13000000 INFO @ Tue, 16 Jun 2020 08:45:24: 24000000 INFO @ Tue, 16 Jun 2020 08:45:26: 19000000 INFO @ Tue, 16 Jun 2020 08:45:29: 14000000 INFO @ Tue, 16 Jun 2020 08:45:30: 25000000 INFO @ Tue, 16 Jun 2020 08:45:32: 20000000 INFO @ Tue, 16 Jun 2020 08:45:35: 15000000 INFO @ Tue, 16 Jun 2020 08:45:36: 26000000 INFO @ Tue, 16 Jun 2020 08:45:38: 21000000 INFO @ Tue, 16 Jun 2020 08:45:40: 16000000 INFO @ Tue, 16 Jun 2020 08:45:41: 27000000 INFO @ Tue, 16 Jun 2020 08:45:44: 22000000 INFO @ Tue, 16 Jun 2020 08:45:46: 17000000 INFO @ Tue, 16 Jun 2020 08:45:47: 28000000 INFO @ Tue, 16 Jun 2020 08:45:49: 23000000 INFO @ Tue, 16 Jun 2020 08:45:52: 18000000 INFO @ Tue, 16 Jun 2020 08:45:53: 29000000 INFO @ Tue, 16 Jun 2020 08:45:55: 24000000 INFO @ Tue, 16 Jun 2020 08:45:58: 19000000 INFO @ Tue, 16 Jun 2020 08:45:59: 30000000 INFO @ Tue, 16 Jun 2020 08:46:01: 25000000 INFO @ Tue, 16 Jun 2020 08:46:03: 20000000 INFO @ Tue, 16 Jun 2020 08:46:04: 31000000 INFO @ Tue, 16 Jun 2020 08:46:06: 26000000 INFO @ Tue, 16 Jun 2020 08:46:09: 21000000 INFO @ Tue, 16 Jun 2020 08:46:10: 32000000 INFO @ Tue, 16 Jun 2020 08:46:12: 27000000 INFO @ Tue, 16 Jun 2020 08:46:15: 22000000 INFO @ Tue, 16 Jun 2020 08:46:16: 33000000 INFO @ Tue, 16 Jun 2020 08:46:18: 28000000 INFO @ Tue, 16 Jun 2020 08:46:21: 23000000 INFO @ Tue, 16 Jun 2020 08:46:22: 34000000 INFO @ Tue, 16 Jun 2020 08:46:22: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:46:22: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:46:22: #1 total tags in treatment: 34045558 INFO @ Tue, 16 Jun 2020 08:46:22: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:46:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:46:23: #1 tags after filtering in treatment: 34045558 INFO @ Tue, 16 Jun 2020 08:46:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:46:23: #1 finished! INFO @ Tue, 16 Jun 2020 08:46:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:46:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:46:24: 29000000 INFO @ Tue, 16 Jun 2020 08:46:25: #2 number of paired peaks: 44 WARNING @ Tue, 16 Jun 2020 08:46:25: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:46:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:46:27: 24000000 INFO @ Tue, 16 Jun 2020 08:46:30: 30000000 INFO @ Tue, 16 Jun 2020 08:46:32: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:46:35: 31000000 INFO @ Tue, 16 Jun 2020 08:46:38: 26000000 INFO @ Tue, 16 Jun 2020 08:46:41: 32000000 INFO @ Tue, 16 Jun 2020 08:46:44: 27000000 INFO @ Tue, 16 Jun 2020 08:46:47: 33000000 INFO @ Tue, 16 Jun 2020 08:46:49: 28000000 INFO @ Tue, 16 Jun 2020 08:46:52: 34000000 INFO @ Tue, 16 Jun 2020 08:46:53: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:46:53: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:46:53: #1 total tags in treatment: 34045558 INFO @ Tue, 16 Jun 2020 08:46:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:46:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:46:53: #1 tags after filtering in treatment: 34045558 INFO @ Tue, 16 Jun 2020 08:46:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:46:53: #1 finished! INFO @ Tue, 16 Jun 2020 08:46:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:46:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:46:55: 29000000 INFO @ Tue, 16 Jun 2020 08:46:55: #2 number of paired peaks: 44 WARNING @ Tue, 16 Jun 2020 08:46:55: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:46:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:47:00: 30000000 INFO @ Tue, 16 Jun 2020 08:47:06: 31000000 INFO @ Tue, 16 Jun 2020 08:47:11: 32000000 INFO @ Tue, 16 Jun 2020 08:47:16: 33000000 INFO @ Tue, 16 Jun 2020 08:47:21: 34000000 INFO @ Tue, 16 Jun 2020 08:47:22: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:47:22: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:47:22: #1 total tags in treatment: 34045558 INFO @ Tue, 16 Jun 2020 08:47:22: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:47:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:47:22: #1 tags after filtering in treatment: 34045558 INFO @ Tue, 16 Jun 2020 08:47:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:47:22: #1 finished! INFO @ Tue, 16 Jun 2020 08:47:22: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:47:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:47:24: #2 number of paired peaks: 44 WARNING @ Tue, 16 Jun 2020 08:47:24: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:47:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2985930/SRX2985930.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。