Job ID = 6367205 SRX = SRX2978709 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-15T23:19:46 prefetch.2.10.7: 1) Downloading 'SRR5799216'... 2020-06-15T23:19:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:22:40 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:22:40 prefetch.2.10.7: 1) 'SRR5799216' was downloaded successfully 2020-06-15T23:22:40 prefetch.2.10.7: 'SRR5799216' has 0 unresolved dependencies Read 27403119 spots for SRR5799216/SRR5799216.sra Written 27403119 spots for SRR5799216/SRR5799216.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:46 27403119 reads; of these: 27403119 (100.00%) were paired; of these: 1013682 (3.70%) aligned concordantly 0 times 22626600 (82.57%) aligned concordantly exactly 1 time 3762837 (13.73%) aligned concordantly >1 times ---- 1013682 pairs aligned concordantly 0 times; of these: 519950 (51.29%) aligned discordantly 1 time ---- 493732 pairs aligned 0 times concordantly or discordantly; of these: 987464 mates make up the pairs; of these: 614288 (62.21%) aligned 0 times 218727 (22.15%) aligned exactly 1 time 154449 (15.64%) aligned >1 times 98.88% overall alignment rate Time searching: 00:23:46 Overall time: 00:23:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 427309 / 26892045 = 0.0159 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:00: 1000000 INFO @ Tue, 16 Jun 2020 09:03:05: 2000000 INFO @ Tue, 16 Jun 2020 09:03:10: 3000000 INFO @ Tue, 16 Jun 2020 09:03:15: 4000000 INFO @ Tue, 16 Jun 2020 09:03:20: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:25: 6000000 INFO @ Tue, 16 Jun 2020 09:03:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:30: 7000000 INFO @ Tue, 16 Jun 2020 09:03:31: 1000000 INFO @ Tue, 16 Jun 2020 09:03:35: 8000000 INFO @ Tue, 16 Jun 2020 09:03:36: 2000000 INFO @ Tue, 16 Jun 2020 09:03:41: 9000000 INFO @ Tue, 16 Jun 2020 09:03:41: 3000000 INFO @ Tue, 16 Jun 2020 09:03:46: 10000000 INFO @ Tue, 16 Jun 2020 09:03:47: 4000000 INFO @ Tue, 16 Jun 2020 09:03:51: 11000000 INFO @ Tue, 16 Jun 2020 09:03:52: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:57: 12000000 INFO @ Tue, 16 Jun 2020 09:03:57: 6000000 INFO @ Tue, 16 Jun 2020 09:04:01: 1000000 INFO @ Tue, 16 Jun 2020 09:04:02: 13000000 INFO @ Tue, 16 Jun 2020 09:04:03: 7000000 INFO @ Tue, 16 Jun 2020 09:04:06: 2000000 INFO @ Tue, 16 Jun 2020 09:04:07: 14000000 INFO @ Tue, 16 Jun 2020 09:04:08: 8000000 INFO @ Tue, 16 Jun 2020 09:04:11: 3000000 INFO @ Tue, 16 Jun 2020 09:04:12: 15000000 INFO @ Tue, 16 Jun 2020 09:04:13: 9000000 INFO @ Tue, 16 Jun 2020 09:04:16: 4000000 INFO @ Tue, 16 Jun 2020 09:04:17: 16000000 INFO @ Tue, 16 Jun 2020 09:04:18: 10000000 INFO @ Tue, 16 Jun 2020 09:04:22: 5000000 INFO @ Tue, 16 Jun 2020 09:04:23: 17000000 INFO @ Tue, 16 Jun 2020 09:04:24: 11000000 INFO @ Tue, 16 Jun 2020 09:04:27: 6000000 INFO @ Tue, 16 Jun 2020 09:04:28: 18000000 INFO @ Tue, 16 Jun 2020 09:04:29: 12000000 INFO @ Tue, 16 Jun 2020 09:04:33: 7000000 INFO @ Tue, 16 Jun 2020 09:04:34: 19000000 INFO @ Tue, 16 Jun 2020 09:04:35: 13000000 INFO @ Tue, 16 Jun 2020 09:04:39: 8000000 INFO @ Tue, 16 Jun 2020 09:04:39: 20000000 INFO @ Tue, 16 Jun 2020 09:04:40: 14000000 INFO @ Tue, 16 Jun 2020 09:04:44: 9000000 INFO @ Tue, 16 Jun 2020 09:04:45: 21000000 INFO @ Tue, 16 Jun 2020 09:04:45: 15000000 INFO @ Tue, 16 Jun 2020 09:04:50: 10000000 INFO @ Tue, 16 Jun 2020 09:04:51: 22000000 INFO @ Tue, 16 Jun 2020 09:04:51: 16000000 INFO @ Tue, 16 Jun 2020 09:04:55: 11000000 INFO @ Tue, 16 Jun 2020 09:04:56: 23000000 INFO @ Tue, 16 Jun 2020 09:04:56: 17000000 INFO @ Tue, 16 Jun 2020 09:05:00: 12000000 INFO @ Tue, 16 Jun 2020 09:05:02: 18000000 INFO @ Tue, 16 Jun 2020 09:05:02: 24000000 INFO @ Tue, 16 Jun 2020 09:05:06: 13000000 INFO @ Tue, 16 Jun 2020 09:05:07: 19000000 INFO @ Tue, 16 Jun 2020 09:05:07: 25000000 INFO @ Tue, 16 Jun 2020 09:05:11: 14000000 INFO @ Tue, 16 Jun 2020 09:05:13: 20000000 INFO @ Tue, 16 Jun 2020 09:05:13: 26000000 INFO @ Tue, 16 Jun 2020 09:05:17: 15000000 INFO @ Tue, 16 Jun 2020 09:05:18: 21000000 INFO @ Tue, 16 Jun 2020 09:05:18: 27000000 INFO @ Tue, 16 Jun 2020 09:05:23: 16000000 INFO @ Tue, 16 Jun 2020 09:05:24: 22000000 INFO @ Tue, 16 Jun 2020 09:05:24: 28000000 INFO @ Tue, 16 Jun 2020 09:05:28: 17000000 INFO @ Tue, 16 Jun 2020 09:05:29: 23000000 INFO @ Tue, 16 Jun 2020 09:05:29: 29000000 INFO @ Tue, 16 Jun 2020 09:05:33: 18000000 INFO @ Tue, 16 Jun 2020 09:05:35: 24000000 INFO @ Tue, 16 Jun 2020 09:05:35: 30000000 INFO @ Tue, 16 Jun 2020 09:05:39: 19000000 INFO @ Tue, 16 Jun 2020 09:05:40: 25000000 INFO @ Tue, 16 Jun 2020 09:05:40: 31000000 INFO @ Tue, 16 Jun 2020 09:05:44: 20000000 INFO @ Tue, 16 Jun 2020 09:05:46: 32000000 INFO @ Tue, 16 Jun 2020 09:05:46: 26000000 INFO @ Tue, 16 Jun 2020 09:05:49: 21000000 INFO @ Tue, 16 Jun 2020 09:05:51: 33000000 INFO @ Tue, 16 Jun 2020 09:05:51: 27000000 INFO @ Tue, 16 Jun 2020 09:05:55: 22000000 INFO @ Tue, 16 Jun 2020 09:05:56: 34000000 INFO @ Tue, 16 Jun 2020 09:05:57: 28000000 INFO @ Tue, 16 Jun 2020 09:06:00: 23000000 INFO @ Tue, 16 Jun 2020 09:06:02: 35000000 INFO @ Tue, 16 Jun 2020 09:06:02: 29000000 INFO @ Tue, 16 Jun 2020 09:06:06: 24000000 INFO @ Tue, 16 Jun 2020 09:06:07: 36000000 INFO @ Tue, 16 Jun 2020 09:06:07: 30000000 INFO @ Tue, 16 Jun 2020 09:06:11: 25000000 INFO @ Tue, 16 Jun 2020 09:06:13: 37000000 INFO @ Tue, 16 Jun 2020 09:06:13: 31000000 INFO @ Tue, 16 Jun 2020 09:06:16: 26000000 INFO @ Tue, 16 Jun 2020 09:06:18: 38000000 INFO @ Tue, 16 Jun 2020 09:06:18: 32000000 INFO @ Tue, 16 Jun 2020 09:06:22: 27000000 INFO @ Tue, 16 Jun 2020 09:06:23: 39000000 INFO @ Tue, 16 Jun 2020 09:06:24: 33000000 INFO @ Tue, 16 Jun 2020 09:06:27: 28000000 INFO @ Tue, 16 Jun 2020 09:06:29: 40000000 INFO @ Tue, 16 Jun 2020 09:06:29: 34000000 INFO @ Tue, 16 Jun 2020 09:06:33: 29000000 INFO @ Tue, 16 Jun 2020 09:06:34: 41000000 INFO @ Tue, 16 Jun 2020 09:06:35: 35000000 INFO @ Tue, 16 Jun 2020 09:06:38: 30000000 INFO @ Tue, 16 Jun 2020 09:06:40: 42000000 INFO @ Tue, 16 Jun 2020 09:06:40: 36000000 INFO @ Tue, 16 Jun 2020 09:06:43: 31000000 INFO @ Tue, 16 Jun 2020 09:06:45: 43000000 INFO @ Tue, 16 Jun 2020 09:06:46: 37000000 INFO @ Tue, 16 Jun 2020 09:06:49: 32000000 INFO @ Tue, 16 Jun 2020 09:06:51: 44000000 INFO @ Tue, 16 Jun 2020 09:06:51: 38000000 INFO @ Tue, 16 Jun 2020 09:06:54: 33000000 INFO @ Tue, 16 Jun 2020 09:06:56: 45000000 INFO @ Tue, 16 Jun 2020 09:06:57: 39000000 INFO @ Tue, 16 Jun 2020 09:07:00: 34000000 INFO @ Tue, 16 Jun 2020 09:07:01: 46000000 INFO @ Tue, 16 Jun 2020 09:07:02: 40000000 INFO @ Tue, 16 Jun 2020 09:07:05: 35000000 INFO @ Tue, 16 Jun 2020 09:07:07: 47000000 INFO @ Tue, 16 Jun 2020 09:07:07: 41000000 INFO @ Tue, 16 Jun 2020 09:07:10: 36000000 INFO @ Tue, 16 Jun 2020 09:07:12: 48000000 INFO @ Tue, 16 Jun 2020 09:07:12: 42000000 INFO @ Tue, 16 Jun 2020 09:07:16: 37000000 INFO @ Tue, 16 Jun 2020 09:07:17: 49000000 INFO @ Tue, 16 Jun 2020 09:07:18: 43000000 INFO @ Tue, 16 Jun 2020 09:07:21: 38000000 INFO @ Tue, 16 Jun 2020 09:07:23: 50000000 INFO @ Tue, 16 Jun 2020 09:07:23: 44000000 INFO @ Tue, 16 Jun 2020 09:07:26: 39000000 INFO @ Tue, 16 Jun 2020 09:07:28: 51000000 INFO @ Tue, 16 Jun 2020 09:07:28: 45000000 INFO @ Tue, 16 Jun 2020 09:07:32: 40000000 INFO @ Tue, 16 Jun 2020 09:07:34: 52000000 INFO @ Tue, 16 Jun 2020 09:07:34: 46000000 INFO @ Tue, 16 Jun 2020 09:07:37: 41000000 INFO @ Tue, 16 Jun 2020 09:07:39: 47000000 INFO @ Tue, 16 Jun 2020 09:07:39: 53000000 INFO @ Tue, 16 Jun 2020 09:07:41: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:07:41: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:07:41: #1 total tags in treatment: 25965119 INFO @ Tue, 16 Jun 2020 09:07:41: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:07:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:07:41: #1 tags after filtering in treatment: 22870846 INFO @ Tue, 16 Jun 2020 09:07:41: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 16 Jun 2020 09:07:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:07:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:07:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:07:42: 42000000 INFO @ Tue, 16 Jun 2020 09:07:43: #2 number of paired peaks: 200 WARNING @ Tue, 16 Jun 2020 09:07:43: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Tue, 16 Jun 2020 09:07:43: start model_add_line... INFO @ Tue, 16 Jun 2020 09:07:43: start X-correlation... INFO @ Tue, 16 Jun 2020 09:07:43: end of X-cor INFO @ Tue, 16 Jun 2020 09:07:43: #2 finished! INFO @ Tue, 16 Jun 2020 09:07:43: #2 predicted fragment length is 81 bps INFO @ Tue, 16 Jun 2020 09:07:43: #2 alternative fragment length(s) may be 2,81,86 bps INFO @ Tue, 16 Jun 2020 09:07:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.05_model.r WARNING @ Tue, 16 Jun 2020 09:07:43: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:07:43: #2 You may need to consider one of the other alternative d(s): 2,81,86 WARNING @ Tue, 16 Jun 2020 09:07:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:07:43: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:07:43: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:07:44: 48000000 INFO @ Tue, 16 Jun 2020 09:07:48: 43000000 INFO @ Tue, 16 Jun 2020 09:07:49: 49000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:07:53: 44000000 INFO @ Tue, 16 Jun 2020 09:07:55: 50000000 INFO @ Tue, 16 Jun 2020 09:07:58: 45000000 INFO @ Tue, 16 Jun 2020 09:08:00: 51000000 INFO @ Tue, 16 Jun 2020 09:08:03: 46000000 INFO @ Tue, 16 Jun 2020 09:08:05: 52000000 INFO @ Tue, 16 Jun 2020 09:08:09: 47000000 INFO @ Tue, 16 Jun 2020 09:08:11: 53000000 INFO @ Tue, 16 Jun 2020 09:08:13: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:08:13: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:08:13: #1 total tags in treatment: 25965119 INFO @ Tue, 16 Jun 2020 09:08:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:08:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:08:13: #1 tags after filtering in treatment: 22870846 INFO @ Tue, 16 Jun 2020 09:08:13: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 16 Jun 2020 09:08:13: #1 finished! INFO @ Tue, 16 Jun 2020 09:08:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:08:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:08:14: 48000000 INFO @ Tue, 16 Jun 2020 09:08:15: #2 number of paired peaks: 200 WARNING @ Tue, 16 Jun 2020 09:08:15: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Tue, 16 Jun 2020 09:08:15: start model_add_line... INFO @ Tue, 16 Jun 2020 09:08:15: start X-correlation... INFO @ Tue, 16 Jun 2020 09:08:15: end of X-cor INFO @ Tue, 16 Jun 2020 09:08:15: #2 finished! INFO @ Tue, 16 Jun 2020 09:08:15: #2 predicted fragment length is 81 bps INFO @ Tue, 16 Jun 2020 09:08:15: #2 alternative fragment length(s) may be 2,81,86 bps INFO @ Tue, 16 Jun 2020 09:08:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.10_model.r WARNING @ Tue, 16 Jun 2020 09:08:15: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:08:15: #2 You may need to consider one of the other alternative d(s): 2,81,86 WARNING @ Tue, 16 Jun 2020 09:08:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:08:15: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:08:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:08:19: 49000000 INFO @ Tue, 16 Jun 2020 09:08:22: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:08:24: 50000000 INFO @ Tue, 16 Jun 2020 09:08:29: 51000000 INFO @ Tue, 16 Jun 2020 09:08:34: 52000000 INFO @ Tue, 16 Jun 2020 09:08:39: 53000000 INFO @ Tue, 16 Jun 2020 09:08:40: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:08:40: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:08:40: #1 total tags in treatment: 25965119 INFO @ Tue, 16 Jun 2020 09:08:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:08:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:08:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:08:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:08:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.05_summits.bed INFO @ Tue, 16 Jun 2020 09:08:40: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (561 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:08:41: #1 tags after filtering in treatment: 22870846 INFO @ Tue, 16 Jun 2020 09:08:41: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 16 Jun 2020 09:08:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:08:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:08:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:08:42: #2 number of paired peaks: 200 WARNING @ Tue, 16 Jun 2020 09:08:42: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Tue, 16 Jun 2020 09:08:42: start model_add_line... INFO @ Tue, 16 Jun 2020 09:08:42: start X-correlation... INFO @ Tue, 16 Jun 2020 09:08:43: end of X-cor INFO @ Tue, 16 Jun 2020 09:08:43: #2 finished! INFO @ Tue, 16 Jun 2020 09:08:43: #2 predicted fragment length is 81 bps INFO @ Tue, 16 Jun 2020 09:08:43: #2 alternative fragment length(s) may be 2,81,86 bps INFO @ Tue, 16 Jun 2020 09:08:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.20_model.r WARNING @ Tue, 16 Jun 2020 09:08:43: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:08:43: #2 You may need to consider one of the other alternative d(s): 2,81,86 WARNING @ Tue, 16 Jun 2020 09:08:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:08:43: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:08:43: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:08:53: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:09:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:09:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:09:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.10_summits.bed INFO @ Tue, 16 Jun 2020 09:09:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (400 records, 4 fields): 1 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:09:21: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:09:39: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:09:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:09:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978709/SRX2978709.20_summits.bed INFO @ Tue, 16 Jun 2020 09:09:39: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (199 records, 4 fields): 1 millis CompletedMACS2peakCalling