Job ID = 6367204 SRX = SRX2978708 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-15T23:26:21 prefetch.2.10.7: 1) Downloading 'SRR5799215'... 2020-06-15T23:26:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:32:25 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:32:25 prefetch.2.10.7: 1) 'SRR5799215' was downloaded successfully 2020-06-15T23:32:25 prefetch.2.10.7: 'SRR5799215' has 0 unresolved dependencies Read 30870687 spots for SRR5799215/SRR5799215.sra Written 30870687 spots for SRR5799215/SRR5799215.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:56 30870687 reads; of these: 30870687 (100.00%) were paired; of these: 2571202 (8.33%) aligned concordantly 0 times 23947324 (77.57%) aligned concordantly exactly 1 time 4352161 (14.10%) aligned concordantly >1 times ---- 2571202 pairs aligned concordantly 0 times; of these: 605851 (23.56%) aligned discordantly 1 time ---- 1965351 pairs aligned 0 times concordantly or discordantly; of these: 3930702 mates make up the pairs; of these: 2527028 (64.29%) aligned 0 times 973317 (24.76%) aligned exactly 1 time 430357 (10.95%) aligned >1 times 95.91% overall alignment rate Time searching: 00:26:57 Overall time: 00:26:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 889354 / 28629107 = 0.0311 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:17:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:17:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:17:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:17:47: 1000000 INFO @ Tue, 16 Jun 2020 09:17:52: 2000000 INFO @ Tue, 16 Jun 2020 09:17:57: 3000000 INFO @ Tue, 16 Jun 2020 09:18:02: 4000000 INFO @ Tue, 16 Jun 2020 09:18:07: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:18:12: 6000000 INFO @ Tue, 16 Jun 2020 09:18:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:18:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:18:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:18:17: 1000000 INFO @ Tue, 16 Jun 2020 09:18:17: 7000000 INFO @ Tue, 16 Jun 2020 09:18:22: 2000000 INFO @ Tue, 16 Jun 2020 09:18:22: 8000000 INFO @ Tue, 16 Jun 2020 09:18:27: 3000000 INFO @ Tue, 16 Jun 2020 09:18:27: 9000000 INFO @ Tue, 16 Jun 2020 09:18:32: 10000000 INFO @ Tue, 16 Jun 2020 09:18:32: 4000000 INFO @ Tue, 16 Jun 2020 09:18:37: 5000000 INFO @ Tue, 16 Jun 2020 09:18:37: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:18:42: 6000000 INFO @ Tue, 16 Jun 2020 09:18:42: 12000000 INFO @ Tue, 16 Jun 2020 09:18:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:18:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:18:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:18:47: 7000000 INFO @ Tue, 16 Jun 2020 09:18:47: 13000000 INFO @ Tue, 16 Jun 2020 09:18:47: 1000000 INFO @ Tue, 16 Jun 2020 09:18:52: 8000000 INFO @ Tue, 16 Jun 2020 09:18:52: 14000000 INFO @ Tue, 16 Jun 2020 09:18:53: 2000000 INFO @ Tue, 16 Jun 2020 09:18:57: 9000000 INFO @ Tue, 16 Jun 2020 09:18:57: 15000000 INFO @ Tue, 16 Jun 2020 09:18:58: 3000000 INFO @ Tue, 16 Jun 2020 09:19:02: 10000000 INFO @ Tue, 16 Jun 2020 09:19:02: 16000000 INFO @ Tue, 16 Jun 2020 09:19:03: 4000000 INFO @ Tue, 16 Jun 2020 09:19:07: 11000000 INFO @ Tue, 16 Jun 2020 09:19:08: 17000000 INFO @ Tue, 16 Jun 2020 09:19:08: 5000000 INFO @ Tue, 16 Jun 2020 09:19:12: 12000000 INFO @ Tue, 16 Jun 2020 09:19:13: 18000000 INFO @ Tue, 16 Jun 2020 09:19:13: 6000000 INFO @ Tue, 16 Jun 2020 09:19:18: 13000000 INFO @ Tue, 16 Jun 2020 09:19:18: 19000000 INFO @ Tue, 16 Jun 2020 09:19:18: 7000000 INFO @ Tue, 16 Jun 2020 09:19:23: 14000000 INFO @ Tue, 16 Jun 2020 09:19:23: 20000000 INFO @ Tue, 16 Jun 2020 09:19:24: 8000000 INFO @ Tue, 16 Jun 2020 09:19:28: 21000000 INFO @ Tue, 16 Jun 2020 09:19:28: 15000000 INFO @ Tue, 16 Jun 2020 09:19:29: 9000000 INFO @ Tue, 16 Jun 2020 09:19:33: 22000000 INFO @ Tue, 16 Jun 2020 09:19:33: 16000000 INFO @ Tue, 16 Jun 2020 09:19:34: 10000000 INFO @ Tue, 16 Jun 2020 09:19:38: 23000000 INFO @ Tue, 16 Jun 2020 09:19:39: 17000000 INFO @ Tue, 16 Jun 2020 09:19:39: 11000000 INFO @ Tue, 16 Jun 2020 09:19:44: 24000000 INFO @ Tue, 16 Jun 2020 09:19:44: 18000000 INFO @ Tue, 16 Jun 2020 09:19:44: 12000000 INFO @ Tue, 16 Jun 2020 09:19:49: 25000000 INFO @ Tue, 16 Jun 2020 09:19:49: 19000000 INFO @ Tue, 16 Jun 2020 09:19:49: 13000000 INFO @ Tue, 16 Jun 2020 09:19:54: 26000000 INFO @ Tue, 16 Jun 2020 09:19:55: 20000000 INFO @ Tue, 16 Jun 2020 09:19:55: 14000000 INFO @ Tue, 16 Jun 2020 09:19:59: 27000000 INFO @ Tue, 16 Jun 2020 09:20:00: 21000000 INFO @ Tue, 16 Jun 2020 09:20:00: 15000000 INFO @ Tue, 16 Jun 2020 09:20:04: 28000000 INFO @ Tue, 16 Jun 2020 09:20:05: 16000000 INFO @ Tue, 16 Jun 2020 09:20:05: 22000000 INFO @ Tue, 16 Jun 2020 09:20:09: 29000000 INFO @ Tue, 16 Jun 2020 09:20:10: 17000000 INFO @ Tue, 16 Jun 2020 09:20:10: 23000000 INFO @ Tue, 16 Jun 2020 09:20:15: 30000000 INFO @ Tue, 16 Jun 2020 09:20:15: 18000000 INFO @ Tue, 16 Jun 2020 09:20:16: 24000000 INFO @ Tue, 16 Jun 2020 09:20:20: 31000000 INFO @ Tue, 16 Jun 2020 09:20:21: 19000000 INFO @ Tue, 16 Jun 2020 09:20:21: 25000000 INFO @ Tue, 16 Jun 2020 09:20:25: 32000000 INFO @ Tue, 16 Jun 2020 09:20:26: 20000000 INFO @ Tue, 16 Jun 2020 09:20:26: 26000000 INFO @ Tue, 16 Jun 2020 09:20:30: 33000000 INFO @ Tue, 16 Jun 2020 09:20:31: 21000000 INFO @ Tue, 16 Jun 2020 09:20:31: 27000000 INFO @ Tue, 16 Jun 2020 09:20:35: 34000000 INFO @ Tue, 16 Jun 2020 09:20:36: 22000000 INFO @ Tue, 16 Jun 2020 09:20:36: 28000000 INFO @ Tue, 16 Jun 2020 09:20:41: 35000000 INFO @ Tue, 16 Jun 2020 09:20:41: 23000000 INFO @ Tue, 16 Jun 2020 09:20:42: 29000000 INFO @ Tue, 16 Jun 2020 09:20:46: 36000000 INFO @ Tue, 16 Jun 2020 09:20:47: 24000000 INFO @ Tue, 16 Jun 2020 09:20:47: 30000000 INFO @ Tue, 16 Jun 2020 09:20:51: 37000000 INFO @ Tue, 16 Jun 2020 09:20:52: 31000000 INFO @ Tue, 16 Jun 2020 09:20:52: 25000000 INFO @ Tue, 16 Jun 2020 09:20:56: 38000000 INFO @ Tue, 16 Jun 2020 09:20:57: 32000000 INFO @ Tue, 16 Jun 2020 09:20:57: 26000000 INFO @ Tue, 16 Jun 2020 09:21:01: 39000000 INFO @ Tue, 16 Jun 2020 09:21:02: 33000000 INFO @ Tue, 16 Jun 2020 09:21:02: 27000000 INFO @ Tue, 16 Jun 2020 09:21:06: 40000000 INFO @ Tue, 16 Jun 2020 09:21:07: 34000000 INFO @ Tue, 16 Jun 2020 09:21:07: 28000000 INFO @ Tue, 16 Jun 2020 09:21:11: 41000000 INFO @ Tue, 16 Jun 2020 09:21:12: 35000000 INFO @ Tue, 16 Jun 2020 09:21:12: 29000000 INFO @ Tue, 16 Jun 2020 09:21:16: 42000000 INFO @ Tue, 16 Jun 2020 09:21:16: 36000000 INFO @ Tue, 16 Jun 2020 09:21:17: 30000000 INFO @ Tue, 16 Jun 2020 09:21:21: 43000000 INFO @ Tue, 16 Jun 2020 09:21:21: 37000000 INFO @ Tue, 16 Jun 2020 09:21:22: 31000000 INFO @ Tue, 16 Jun 2020 09:21:26: 38000000 INFO @ Tue, 16 Jun 2020 09:21:26: 44000000 INFO @ Tue, 16 Jun 2020 09:21:27: 32000000 INFO @ Tue, 16 Jun 2020 09:21:31: 39000000 INFO @ Tue, 16 Jun 2020 09:21:31: 45000000 INFO @ Tue, 16 Jun 2020 09:21:33: 33000000 INFO @ Tue, 16 Jun 2020 09:21:36: 40000000 INFO @ Tue, 16 Jun 2020 09:21:36: 46000000 INFO @ Tue, 16 Jun 2020 09:21:38: 34000000 INFO @ Tue, 16 Jun 2020 09:21:41: 41000000 INFO @ Tue, 16 Jun 2020 09:21:42: 47000000 INFO @ Tue, 16 Jun 2020 09:21:43: 35000000 INFO @ Tue, 16 Jun 2020 09:21:46: 42000000 INFO @ Tue, 16 Jun 2020 09:21:47: 48000000 INFO @ Tue, 16 Jun 2020 09:21:48: 36000000 INFO @ Tue, 16 Jun 2020 09:21:51: 43000000 INFO @ Tue, 16 Jun 2020 09:21:52: 49000000 INFO @ Tue, 16 Jun 2020 09:21:54: 37000000 INFO @ Tue, 16 Jun 2020 09:21:56: 44000000 INFO @ Tue, 16 Jun 2020 09:21:57: 50000000 INFO @ Tue, 16 Jun 2020 09:21:59: 38000000 INFO @ Tue, 16 Jun 2020 09:22:02: 45000000 INFO @ Tue, 16 Jun 2020 09:22:02: 51000000 INFO @ Tue, 16 Jun 2020 09:22:04: 39000000 INFO @ Tue, 16 Jun 2020 09:22:07: 46000000 INFO @ Tue, 16 Jun 2020 09:22:07: 52000000 INFO @ Tue, 16 Jun 2020 09:22:09: 40000000 INFO @ Tue, 16 Jun 2020 09:22:12: 47000000 INFO @ Tue, 16 Jun 2020 09:22:13: 53000000 INFO @ Tue, 16 Jun 2020 09:22:15: 41000000 INFO @ Tue, 16 Jun 2020 09:22:17: 48000000 INFO @ Tue, 16 Jun 2020 09:22:18: 54000000 INFO @ Tue, 16 Jun 2020 09:22:20: 42000000 INFO @ Tue, 16 Jun 2020 09:22:22: 49000000 INFO @ Tue, 16 Jun 2020 09:22:23: 55000000 INFO @ Tue, 16 Jun 2020 09:22:25: 43000000 INFO @ Tue, 16 Jun 2020 09:22:27: 50000000 INFO @ Tue, 16 Jun 2020 09:22:29: 56000000 INFO @ Tue, 16 Jun 2020 09:22:30: 44000000 INFO @ Tue, 16 Jun 2020 09:22:32: 51000000 INFO @ Tue, 16 Jun 2020 09:22:34: 57000000 INFO @ Tue, 16 Jun 2020 09:22:36: 45000000 INFO @ Tue, 16 Jun 2020 09:22:36: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:22:36: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:22:36: #1 total tags in treatment: 27412858 INFO @ Tue, 16 Jun 2020 09:22:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:22:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:22:37: #1 tags after filtering in treatment: 23868829 INFO @ Tue, 16 Jun 2020 09:22:37: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 16 Jun 2020 09:22:37: #1 finished! INFO @ Tue, 16 Jun 2020 09:22:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:22:37: 52000000 INFO @ Tue, 16 Jun 2020 09:22:39: #2 number of paired peaks: 199 WARNING @ Tue, 16 Jun 2020 09:22:39: Fewer paired peaks (199) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 199 pairs to build model! INFO @ Tue, 16 Jun 2020 09:22:39: start model_add_line... INFO @ Tue, 16 Jun 2020 09:22:39: start X-correlation... INFO @ Tue, 16 Jun 2020 09:22:39: end of X-cor INFO @ Tue, 16 Jun 2020 09:22:39: #2 finished! INFO @ Tue, 16 Jun 2020 09:22:39: #2 predicted fragment length is 97 bps INFO @ Tue, 16 Jun 2020 09:22:39: #2 alternative fragment length(s) may be 2,72,97 bps INFO @ Tue, 16 Jun 2020 09:22:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.05_model.r WARNING @ Tue, 16 Jun 2020 09:22:39: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:22:39: #2 You may need to consider one of the other alternative d(s): 2,72,97 WARNING @ Tue, 16 Jun 2020 09:22:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:22:39: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:22:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:22:41: 46000000 INFO @ Tue, 16 Jun 2020 09:22:42: 53000000 INFO @ Tue, 16 Jun 2020 09:22:47: 47000000 INFO @ Tue, 16 Jun 2020 09:22:47: 54000000 INFO @ Tue, 16 Jun 2020 09:22:52: 48000000 INFO @ Tue, 16 Jun 2020 09:22:53: 55000000 INFO @ Tue, 16 Jun 2020 09:22:57: 49000000 INFO @ Tue, 16 Jun 2020 09:22:58: 56000000 INFO @ Tue, 16 Jun 2020 09:23:03: 50000000 INFO @ Tue, 16 Jun 2020 09:23:04: 57000000 INFO @ Tue, 16 Jun 2020 09:23:06: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:23:06: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:23:06: #1 total tags in treatment: 27412858 INFO @ Tue, 16 Jun 2020 09:23:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:23:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:23:07: #1 tags after filtering in treatment: 23868829 INFO @ Tue, 16 Jun 2020 09:23:07: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 16 Jun 2020 09:23:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:23:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:23:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:23:08: 51000000 INFO @ Tue, 16 Jun 2020 09:23:09: #2 number of paired peaks: 199 WARNING @ Tue, 16 Jun 2020 09:23:09: Fewer paired peaks (199) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 199 pairs to build model! INFO @ Tue, 16 Jun 2020 09:23:09: start model_add_line... INFO @ Tue, 16 Jun 2020 09:23:09: start X-correlation... INFO @ Tue, 16 Jun 2020 09:23:09: end of X-cor INFO @ Tue, 16 Jun 2020 09:23:09: #2 finished! INFO @ Tue, 16 Jun 2020 09:23:09: #2 predicted fragment length is 97 bps INFO @ Tue, 16 Jun 2020 09:23:09: #2 alternative fragment length(s) may be 2,72,97 bps INFO @ Tue, 16 Jun 2020 09:23:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.10_model.r WARNING @ Tue, 16 Jun 2020 09:23:09: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:23:09: #2 You may need to consider one of the other alternative d(s): 2,72,97 WARNING @ Tue, 16 Jun 2020 09:23:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:23:09: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:23:09: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:23:14: 52000000 INFO @ Tue, 16 Jun 2020 09:23:19: 53000000 INFO @ Tue, 16 Jun 2020 09:23:20: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:23:24: 54000000 INFO @ Tue, 16 Jun 2020 09:23:29: 55000000 INFO @ Tue, 16 Jun 2020 09:23:35: 56000000 INFO @ Tue, 16 Jun 2020 09:23:40: 57000000 INFO @ Tue, 16 Jun 2020 09:23:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:23:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:23:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.05_summits.bed INFO @ Tue, 16 Jun 2020 09:23:40: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (936 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:23:42: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:23:42: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:23:42: #1 total tags in treatment: 27412858 INFO @ Tue, 16 Jun 2020 09:23:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:23:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:23:43: #1 tags after filtering in treatment: 23868829 INFO @ Tue, 16 Jun 2020 09:23:43: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 16 Jun 2020 09:23:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:23:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:23:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:23:44: #2 number of paired peaks: 199 WARNING @ Tue, 16 Jun 2020 09:23:44: Fewer paired peaks (199) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 199 pairs to build model! INFO @ Tue, 16 Jun 2020 09:23:44: start model_add_line... INFO @ Tue, 16 Jun 2020 09:23:45: start X-correlation... INFO @ Tue, 16 Jun 2020 09:23:45: end of X-cor INFO @ Tue, 16 Jun 2020 09:23:45: #2 finished! INFO @ Tue, 16 Jun 2020 09:23:45: #2 predicted fragment length is 97 bps INFO @ Tue, 16 Jun 2020 09:23:45: #2 alternative fragment length(s) may be 2,72,97 bps INFO @ Tue, 16 Jun 2020 09:23:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.20_model.r WARNING @ Tue, 16 Jun 2020 09:23:45: #2 Since the d (97) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 09:23:45: #2 You may need to consider one of the other alternative d(s): 2,72,97 WARNING @ Tue, 16 Jun 2020 09:23:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 09:23:45: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:23:45: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:23:49: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:24:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:24:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:24:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.10_summits.bed INFO @ Tue, 16 Jun 2020 09:24:10: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (384 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:24:26: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:24:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:24:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:24:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX2978708/SRX2978708.20_summits.bed INFO @ Tue, 16 Jun 2020 09:24:47: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (211 records, 4 fields): 1 millis CompletedMACS2peakCalling