Job ID = 6367183 SRX = SRX2973389 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:26:36 prefetch.2.10.7: 1) Downloading 'SRR5786980'... 2020-06-15T23:26:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:37:39 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:37:39 prefetch.2.10.7: 1) 'SRR5786980' was downloaded successfully Read 45310834 spots for SRR5786980/SRR5786980.sra Written 45310834 spots for SRR5786980/SRR5786980.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:52 45310834 reads; of these: 45310834 (100.00%) were unpaired; of these: 9131022 (20.15%) aligned 0 times 31241245 (68.95%) aligned exactly 1 time 4938567 (10.90%) aligned >1 times 79.85% overall alignment rate Time searching: 00:17:52 Overall time: 00:17:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6697022 / 36179812 = 0.1851 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:10:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:10:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:10:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:10:32: 1000000 INFO @ Tue, 16 Jun 2020 09:10:40: 2000000 INFO @ Tue, 16 Jun 2020 09:10:47: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:10:54: 4000000 INFO @ Tue, 16 Jun 2020 09:10:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:10:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:10:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:02: 5000000 INFO @ Tue, 16 Jun 2020 09:11:03: 1000000 INFO @ Tue, 16 Jun 2020 09:11:10: 6000000 INFO @ Tue, 16 Jun 2020 09:11:12: 2000000 INFO @ Tue, 16 Jun 2020 09:11:18: 7000000 INFO @ Tue, 16 Jun 2020 09:11:20: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:11:27: 8000000 INFO @ Tue, 16 Jun 2020 09:11:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:11:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:11:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:11:28: 4000000 INFO @ Tue, 16 Jun 2020 09:11:35: 9000000 INFO @ Tue, 16 Jun 2020 09:11:35: 1000000 INFO @ Tue, 16 Jun 2020 09:11:36: 5000000 INFO @ Tue, 16 Jun 2020 09:11:44: 10000000 INFO @ Tue, 16 Jun 2020 09:11:44: 2000000 INFO @ Tue, 16 Jun 2020 09:11:45: 6000000 INFO @ Tue, 16 Jun 2020 09:11:52: 11000000 INFO @ Tue, 16 Jun 2020 09:11:52: 3000000 INFO @ Tue, 16 Jun 2020 09:11:53: 7000000 INFO @ Tue, 16 Jun 2020 09:12:01: 12000000 INFO @ Tue, 16 Jun 2020 09:12:01: 4000000 INFO @ Tue, 16 Jun 2020 09:12:02: 8000000 INFO @ Tue, 16 Jun 2020 09:12:09: 13000000 INFO @ Tue, 16 Jun 2020 09:12:09: 5000000 INFO @ Tue, 16 Jun 2020 09:12:10: 9000000 INFO @ Tue, 16 Jun 2020 09:12:18: 14000000 INFO @ Tue, 16 Jun 2020 09:12:18: 6000000 INFO @ Tue, 16 Jun 2020 09:12:19: 10000000 INFO @ Tue, 16 Jun 2020 09:12:26: 15000000 INFO @ Tue, 16 Jun 2020 09:12:26: 7000000 INFO @ Tue, 16 Jun 2020 09:12:27: 11000000 INFO @ Tue, 16 Jun 2020 09:12:35: 16000000 INFO @ Tue, 16 Jun 2020 09:12:35: 8000000 INFO @ Tue, 16 Jun 2020 09:12:36: 12000000 INFO @ Tue, 16 Jun 2020 09:12:43: 17000000 INFO @ Tue, 16 Jun 2020 09:12:43: 9000000 INFO @ Tue, 16 Jun 2020 09:12:44: 13000000 INFO @ Tue, 16 Jun 2020 09:12:51: 18000000 INFO @ Tue, 16 Jun 2020 09:12:52: 10000000 INFO @ Tue, 16 Jun 2020 09:12:53: 14000000 INFO @ Tue, 16 Jun 2020 09:13:00: 19000000 INFO @ Tue, 16 Jun 2020 09:13:00: 11000000 INFO @ Tue, 16 Jun 2020 09:13:02: 15000000 INFO @ Tue, 16 Jun 2020 09:13:08: 20000000 INFO @ Tue, 16 Jun 2020 09:13:09: 12000000 INFO @ Tue, 16 Jun 2020 09:13:10: 16000000 INFO @ Tue, 16 Jun 2020 09:13:17: 21000000 INFO @ Tue, 16 Jun 2020 09:13:17: 13000000 INFO @ Tue, 16 Jun 2020 09:13:18: 17000000 INFO @ Tue, 16 Jun 2020 09:13:25: 14000000 INFO @ Tue, 16 Jun 2020 09:13:25: 22000000 INFO @ Tue, 16 Jun 2020 09:13:27: 18000000 INFO @ Tue, 16 Jun 2020 09:13:34: 23000000 INFO @ Tue, 16 Jun 2020 09:13:34: 15000000 INFO @ Tue, 16 Jun 2020 09:13:35: 19000000 INFO @ Tue, 16 Jun 2020 09:13:42: 24000000 INFO @ Tue, 16 Jun 2020 09:13:42: 16000000 INFO @ Tue, 16 Jun 2020 09:13:43: 20000000 INFO @ Tue, 16 Jun 2020 09:13:50: 17000000 INFO @ Tue, 16 Jun 2020 09:13:51: 25000000 INFO @ Tue, 16 Jun 2020 09:13:52: 21000000 INFO @ Tue, 16 Jun 2020 09:13:58: 18000000 INFO @ Tue, 16 Jun 2020 09:13:59: 26000000 INFO @ Tue, 16 Jun 2020 09:14:00: 22000000 INFO @ Tue, 16 Jun 2020 09:14:07: 19000000 INFO @ Tue, 16 Jun 2020 09:14:07: 27000000 INFO @ Tue, 16 Jun 2020 09:14:08: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:14:15: 20000000 INFO @ Tue, 16 Jun 2020 09:14:15: 28000000 INFO @ Tue, 16 Jun 2020 09:14:16: 24000000 INFO @ Tue, 16 Jun 2020 09:14:23: 29000000 INFO @ Tue, 16 Jun 2020 09:14:23: 21000000 INFO @ Tue, 16 Jun 2020 09:14:24: 25000000 INFO @ Tue, 16 Jun 2020 09:14:27: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:14:27: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:14:27: #1 total tags in treatment: 29482790 INFO @ Tue, 16 Jun 2020 09:14:27: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:28: #1 tags after filtering in treatment: 29482790 INFO @ Tue, 16 Jun 2020 09:14:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:28: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:30: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 09:14:30: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:14:31: 22000000 INFO @ Tue, 16 Jun 2020 09:14:32: 26000000 INFO @ Tue, 16 Jun 2020 09:14:39: 23000000 INFO @ Tue, 16 Jun 2020 09:14:40: 27000000 INFO @ Tue, 16 Jun 2020 09:14:47: 24000000 INFO @ Tue, 16 Jun 2020 09:14:48: 28000000 INFO @ Tue, 16 Jun 2020 09:14:55: 25000000 INFO @ Tue, 16 Jun 2020 09:14:56: 29000000 INFO @ Tue, 16 Jun 2020 09:15:00: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:15:00: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:15:00: #1 total tags in treatment: 29482790 INFO @ Tue, 16 Jun 2020 09:15:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:15:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:15:01: #1 tags after filtering in treatment: 29482790 INFO @ Tue, 16 Jun 2020 09:15:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:15:01: #1 finished! INFO @ Tue, 16 Jun 2020 09:15:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:15:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:15:03: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 09:15:03: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:15:03: Process for pairing-model is terminated! INFO @ Tue, 16 Jun 2020 09:15:03: 26000000 cut: /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:15:10: 27000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:15:17: 28000000 INFO @ Tue, 16 Jun 2020 09:15:24: 29000000 INFO @ Tue, 16 Jun 2020 09:15:28: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:15:28: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:15:28: #1 total tags in treatment: 29482790 INFO @ Tue, 16 Jun 2020 09:15:28: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:15:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:15:28: #1 tags after filtering in treatment: 29482790 INFO @ Tue, 16 Jun 2020 09:15:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:15:28: #1 finished! INFO @ Tue, 16 Jun 2020 09:15:28: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:15:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:15:30: #2 number of paired peaks: 47 WARNING @ Tue, 16 Jun 2020 09:15:30: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:15:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973389/SRX2973389.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling