Job ID = 6367182 SRX = SRX2973388 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:19:31 prefetch.2.10.7: 1) Downloading 'SRR5786981'... 2020-06-15T23:19:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:25:20 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:25:20 prefetch.2.10.7: 1) 'SRR5786981' was downloaded successfully 2020-06-15T23:25:20 prefetch.2.10.7: 'SRR5786981' has 0 unresolved dependencies Read 81489952 spots for SRR5786981/SRR5786981.sra Written 81489952 spots for SRR5786981/SRR5786981.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:05 81489952 reads; of these: 81489952 (100.00%) were unpaired; of these: 3649171 (4.48%) aligned 0 times 64462508 (79.10%) aligned exactly 1 time 13378273 (16.42%) aligned >1 times 95.52% overall alignment rate Time searching: 00:29:05 Overall time: 00:29:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 21553632 / 77840781 = 0.2769 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:16:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:16:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:16:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:16:25: 1000000 INFO @ Tue, 16 Jun 2020 09:16:33: 2000000 INFO @ Tue, 16 Jun 2020 09:16:41: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:16:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:16:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:16:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:16:49: 4000000 INFO @ Tue, 16 Jun 2020 09:16:54: 1000000 INFO @ Tue, 16 Jun 2020 09:16:57: 5000000 INFO @ Tue, 16 Jun 2020 09:17:02: 2000000 INFO @ Tue, 16 Jun 2020 09:17:05: 6000000 INFO @ Tue, 16 Jun 2020 09:17:09: 3000000 INFO @ Tue, 16 Jun 2020 09:17:14: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:17:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:17:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:17:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:17:17: 4000000 INFO @ Tue, 16 Jun 2020 09:17:22: 8000000 INFO @ Tue, 16 Jun 2020 09:17:25: 1000000 INFO @ Tue, 16 Jun 2020 09:17:25: 5000000 INFO @ Tue, 16 Jun 2020 09:17:31: 9000000 INFO @ Tue, 16 Jun 2020 09:17:33: 2000000 INFO @ Tue, 16 Jun 2020 09:17:33: 6000000 INFO @ Tue, 16 Jun 2020 09:17:39: 10000000 INFO @ Tue, 16 Jun 2020 09:17:41: 3000000 INFO @ Tue, 16 Jun 2020 09:17:41: 7000000 INFO @ Tue, 16 Jun 2020 09:17:48: 11000000 INFO @ Tue, 16 Jun 2020 09:17:48: 4000000 INFO @ Tue, 16 Jun 2020 09:17:49: 8000000 INFO @ Tue, 16 Jun 2020 09:17:56: 5000000 INFO @ Tue, 16 Jun 2020 09:17:57: 9000000 INFO @ Tue, 16 Jun 2020 09:17:57: 12000000 INFO @ Tue, 16 Jun 2020 09:18:04: 6000000 INFO @ Tue, 16 Jun 2020 09:18:05: 10000000 INFO @ Tue, 16 Jun 2020 09:18:05: 13000000 INFO @ Tue, 16 Jun 2020 09:18:12: 7000000 INFO @ Tue, 16 Jun 2020 09:18:13: 11000000 INFO @ Tue, 16 Jun 2020 09:18:13: 14000000 INFO @ Tue, 16 Jun 2020 09:18:19: 8000000 INFO @ Tue, 16 Jun 2020 09:18:20: 12000000 INFO @ Tue, 16 Jun 2020 09:18:22: 15000000 INFO @ Tue, 16 Jun 2020 09:18:27: 9000000 INFO @ Tue, 16 Jun 2020 09:18:28: 13000000 INFO @ Tue, 16 Jun 2020 09:18:30: 16000000 INFO @ Tue, 16 Jun 2020 09:18:35: 10000000 INFO @ Tue, 16 Jun 2020 09:18:36: 14000000 INFO @ Tue, 16 Jun 2020 09:18:38: 17000000 INFO @ Tue, 16 Jun 2020 09:18:43: 11000000 INFO @ Tue, 16 Jun 2020 09:18:44: 15000000 INFO @ Tue, 16 Jun 2020 09:18:47: 18000000 INFO @ Tue, 16 Jun 2020 09:18:51: 12000000 INFO @ Tue, 16 Jun 2020 09:18:52: 16000000 INFO @ Tue, 16 Jun 2020 09:18:55: 19000000 INFO @ Tue, 16 Jun 2020 09:18:58: 13000000 INFO @ Tue, 16 Jun 2020 09:18:59: 17000000 INFO @ Tue, 16 Jun 2020 09:19:03: 20000000 INFO @ Tue, 16 Jun 2020 09:19:06: 14000000 INFO @ Tue, 16 Jun 2020 09:19:07: 18000000 INFO @ Tue, 16 Jun 2020 09:19:11: 21000000 INFO @ Tue, 16 Jun 2020 09:19:13: 15000000 INFO @ Tue, 16 Jun 2020 09:19:15: 19000000 INFO @ Tue, 16 Jun 2020 09:19:19: 22000000 INFO @ Tue, 16 Jun 2020 09:19:21: 16000000 INFO @ Tue, 16 Jun 2020 09:19:23: 20000000 INFO @ Tue, 16 Jun 2020 09:19:27: 23000000 INFO @ Tue, 16 Jun 2020 09:19:29: 17000000 INFO @ Tue, 16 Jun 2020 09:19:31: 21000000 INFO @ Tue, 16 Jun 2020 09:19:35: 24000000 INFO @ Tue, 16 Jun 2020 09:19:37: 18000000 INFO @ Tue, 16 Jun 2020 09:19:38: 22000000 INFO @ Tue, 16 Jun 2020 09:19:44: 25000000 INFO @ Tue, 16 Jun 2020 09:19:45: 19000000 INFO @ Tue, 16 Jun 2020 09:19:47: 23000000 INFO @ Tue, 16 Jun 2020 09:19:52: 26000000 INFO @ Tue, 16 Jun 2020 09:19:53: 20000000 INFO @ Tue, 16 Jun 2020 09:19:55: 24000000 INFO @ Tue, 16 Jun 2020 09:20:01: 27000000 INFO @ Tue, 16 Jun 2020 09:20:01: 21000000 INFO @ Tue, 16 Jun 2020 09:20:02: 25000000 INFO @ Tue, 16 Jun 2020 09:20:09: 22000000 INFO @ Tue, 16 Jun 2020 09:20:09: 28000000 INFO @ Tue, 16 Jun 2020 09:20:10: 26000000 INFO @ Tue, 16 Jun 2020 09:20:16: 23000000 INFO @ Tue, 16 Jun 2020 09:20:18: 29000000 INFO @ Tue, 16 Jun 2020 09:20:18: 27000000 INFO @ Tue, 16 Jun 2020 09:20:24: 24000000 INFO @ Tue, 16 Jun 2020 09:20:26: 28000000 INFO @ Tue, 16 Jun 2020 09:20:27: 30000000 INFO @ Tue, 16 Jun 2020 09:20:32: 25000000 INFO @ Tue, 16 Jun 2020 09:20:34: 29000000 INFO @ Tue, 16 Jun 2020 09:20:35: 31000000 INFO @ Tue, 16 Jun 2020 09:20:40: 26000000 INFO @ Tue, 16 Jun 2020 09:20:42: 30000000 INFO @ Tue, 16 Jun 2020 09:20:43: 32000000 INFO @ Tue, 16 Jun 2020 09:20:47: 27000000 INFO @ Tue, 16 Jun 2020 09:20:50: 31000000 INFO @ Tue, 16 Jun 2020 09:20:52: 33000000 INFO @ Tue, 16 Jun 2020 09:20:55: 28000000 INFO @ Tue, 16 Jun 2020 09:20:57: 32000000 INFO @ Tue, 16 Jun 2020 09:21:00: 34000000 INFO @ Tue, 16 Jun 2020 09:21:02: 29000000 INFO @ Tue, 16 Jun 2020 09:21:05: 33000000 INFO @ Tue, 16 Jun 2020 09:21:08: 35000000 INFO @ Tue, 16 Jun 2020 09:21:10: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:21:13: 34000000 INFO @ Tue, 16 Jun 2020 09:21:16: 36000000 INFO @ Tue, 16 Jun 2020 09:21:18: 31000000 INFO @ Tue, 16 Jun 2020 09:21:20: 35000000 INFO @ Tue, 16 Jun 2020 09:21:24: 37000000 INFO @ Tue, 16 Jun 2020 09:21:25: 32000000 INFO @ Tue, 16 Jun 2020 09:21:28: 36000000 INFO @ Tue, 16 Jun 2020 09:21:31: 38000000 INFO @ Tue, 16 Jun 2020 09:21:33: 33000000 INFO @ Tue, 16 Jun 2020 09:21:35: 37000000 INFO @ Tue, 16 Jun 2020 09:21:39: 39000000 INFO @ Tue, 16 Jun 2020 09:21:40: 34000000 INFO @ Tue, 16 Jun 2020 09:21:43: 38000000 INFO @ Tue, 16 Jun 2020 09:21:47: 40000000 INFO @ Tue, 16 Jun 2020 09:21:48: 35000000 INFO @ Tue, 16 Jun 2020 09:21:50: 39000000 INFO @ Tue, 16 Jun 2020 09:21:55: 41000000 INFO @ Tue, 16 Jun 2020 09:21:55: 36000000 INFO @ Tue, 16 Jun 2020 09:21:57: 40000000 INFO @ Tue, 16 Jun 2020 09:22:02: 42000000 INFO @ Tue, 16 Jun 2020 09:22:02: 37000000 INFO @ Tue, 16 Jun 2020 09:22:05: 41000000 INFO @ Tue, 16 Jun 2020 09:22:10: 38000000 INFO @ Tue, 16 Jun 2020 09:22:10: 43000000 INFO @ Tue, 16 Jun 2020 09:22:12: 42000000 INFO @ Tue, 16 Jun 2020 09:22:17: 39000000 INFO @ Tue, 16 Jun 2020 09:22:18: 44000000 INFO @ Tue, 16 Jun 2020 09:22:20: 43000000 INFO @ Tue, 16 Jun 2020 09:22:25: 40000000 INFO @ Tue, 16 Jun 2020 09:22:26: 45000000 INFO @ Tue, 16 Jun 2020 09:22:27: 44000000 INFO @ Tue, 16 Jun 2020 09:22:32: 41000000 INFO @ Tue, 16 Jun 2020 09:22:33: 46000000 INFO @ Tue, 16 Jun 2020 09:22:35: 45000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:22:40: 42000000 INFO @ Tue, 16 Jun 2020 09:22:41: 47000000 INFO @ Tue, 16 Jun 2020 09:22:42: 46000000 INFO @ Tue, 16 Jun 2020 09:22:47: 43000000 INFO @ Tue, 16 Jun 2020 09:22:49: 48000000 INFO @ Tue, 16 Jun 2020 09:22:50: 47000000 INFO @ Tue, 16 Jun 2020 09:22:55: 44000000 INFO @ Tue, 16 Jun 2020 09:22:57: 49000000 INFO @ Tue, 16 Jun 2020 09:22:58: 48000000 INFO @ Tue, 16 Jun 2020 09:23:03: 45000000 INFO @ Tue, 16 Jun 2020 09:23:05: 50000000 INFO @ Tue, 16 Jun 2020 09:23:05: 49000000 INFO @ Tue, 16 Jun 2020 09:23:11: 46000000 INFO @ Tue, 16 Jun 2020 09:23:13: 51000000 INFO @ Tue, 16 Jun 2020 09:23:13: 50000000 INFO @ Tue, 16 Jun 2020 09:23:18: 47000000 INFO @ Tue, 16 Jun 2020 09:23:20: 52000000 INFO @ Tue, 16 Jun 2020 09:23:21: 51000000 INFO @ Tue, 16 Jun 2020 09:23:26: 48000000 INFO @ Tue, 16 Jun 2020 09:23:28: 53000000 INFO @ Tue, 16 Jun 2020 09:23:28: 52000000 INFO @ Tue, 16 Jun 2020 09:23:34: 49000000 INFO @ Tue, 16 Jun 2020 09:23:36: 53000000 INFO @ Tue, 16 Jun 2020 09:23:36: 54000000 INFO @ Tue, 16 Jun 2020 09:23:41: 50000000 INFO @ Tue, 16 Jun 2020 09:23:43: 54000000 INFO @ Tue, 16 Jun 2020 09:23:44: 55000000 INFO @ Tue, 16 Jun 2020 09:23:49: 51000000 INFO @ Tue, 16 Jun 2020 09:23:51: 55000000 INFO @ Tue, 16 Jun 2020 09:23:51: 56000000 INFO @ Tue, 16 Jun 2020 09:23:54: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:23:54: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:23:54: #1 total tags in treatment: 56287149 INFO @ Tue, 16 Jun 2020 09:23:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:23:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:23:55: #1 tags after filtering in treatment: 56287149 INFO @ Tue, 16 Jun 2020 09:23:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:23:55: #1 finished! INFO @ Tue, 16 Jun 2020 09:23:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:23:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:23:56: 52000000 INFO @ Tue, 16 Jun 2020 09:23:58: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:23:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:23:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 16 Jun 2020 09:23:58: 56000000 pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:24:01: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:24:01: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:24:01: #1 total tags in treatment: 56287149 INFO @ Tue, 16 Jun 2020 09:24:01: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:24:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:24:02: #1 tags after filtering in treatment: 56287149 INFO @ Tue, 16 Jun 2020 09:24:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:24:02: #1 finished! INFO @ Tue, 16 Jun 2020 09:24:02: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:24:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:24:03: 53000000 INFO @ Tue, 16 Jun 2020 09:24:06: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:24:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:24:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:24:10: 54000000 INFO @ Tue, 16 Jun 2020 09:24:17: 55000000 INFO @ Tue, 16 Jun 2020 09:24:23: 56000000 INFO @ Tue, 16 Jun 2020 09:24:26: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:24:26: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:24:26: #1 total tags in treatment: 56287149 INFO @ Tue, 16 Jun 2020 09:24:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:24:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:24:27: #1 tags after filtering in treatment: 56287149 INFO @ Tue, 16 Jun 2020 09:24:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:24:27: #1 finished! INFO @ Tue, 16 Jun 2020 09:24:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:24:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:24:30: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:24:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:24:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973388/SRX2973388.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling