Job ID = 6367180 SRX = SRX2973386 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:15:17 prefetch.2.10.7: 1) Downloading 'SRR5786983'... 2020-06-15T23:15:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:20:34 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:20:34 prefetch.2.10.7: 1) 'SRR5786983' was downloaded successfully 2020-06-15T23:20:34 prefetch.2.10.7: 'SRR5786983' has 0 unresolved dependencies Read 71862577 spots for SRR5786983/SRR5786983.sra Written 71862577 spots for SRR5786983/SRR5786983.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:33 71862577 reads; of these: 71862577 (100.00%) were unpaired; of these: 4849057 (6.75%) aligned 0 times 56182502 (78.18%) aligned exactly 1 time 10831018 (15.07%) aligned >1 times 93.25% overall alignment rate Time searching: 00:23:34 Overall time: 00:23:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 22905098 / 67013520 = 0.3418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:02:11: 1000000 INFO @ Tue, 16 Jun 2020 09:02:18: 2000000 INFO @ Tue, 16 Jun 2020 09:02:24: 3000000 INFO @ Tue, 16 Jun 2020 09:02:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:02:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:02:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:02:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:02:37: 5000000 INFO @ Tue, 16 Jun 2020 09:02:42: 1000000 INFO @ Tue, 16 Jun 2020 09:02:45: 6000000 INFO @ Tue, 16 Jun 2020 09:02:50: 2000000 INFO @ Tue, 16 Jun 2020 09:02:52: 7000000 INFO @ Tue, 16 Jun 2020 09:02:57: 3000000 INFO @ Tue, 16 Jun 2020 09:02:59: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:04: 4000000 INFO @ Tue, 16 Jun 2020 09:03:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:07: 9000000 INFO @ Tue, 16 Jun 2020 09:03:12: 5000000 INFO @ Tue, 16 Jun 2020 09:03:13: 1000000 INFO @ Tue, 16 Jun 2020 09:03:14: 10000000 INFO @ Tue, 16 Jun 2020 09:03:20: 6000000 INFO @ Tue, 16 Jun 2020 09:03:20: 2000000 INFO @ Tue, 16 Jun 2020 09:03:22: 11000000 INFO @ Tue, 16 Jun 2020 09:03:27: 7000000 INFO @ Tue, 16 Jun 2020 09:03:28: 3000000 INFO @ Tue, 16 Jun 2020 09:03:29: 12000000 INFO @ Tue, 16 Jun 2020 09:03:35: 8000000 INFO @ Tue, 16 Jun 2020 09:03:35: 4000000 INFO @ Tue, 16 Jun 2020 09:03:37: 13000000 INFO @ Tue, 16 Jun 2020 09:03:42: 9000000 INFO @ Tue, 16 Jun 2020 09:03:43: 5000000 INFO @ Tue, 16 Jun 2020 09:03:44: 14000000 INFO @ Tue, 16 Jun 2020 09:03:50: 10000000 INFO @ Tue, 16 Jun 2020 09:03:51: 6000000 INFO @ Tue, 16 Jun 2020 09:03:52: 15000000 INFO @ Tue, 16 Jun 2020 09:03:58: 11000000 INFO @ Tue, 16 Jun 2020 09:03:58: 7000000 INFO @ Tue, 16 Jun 2020 09:03:59: 16000000 INFO @ Tue, 16 Jun 2020 09:04:05: 12000000 INFO @ Tue, 16 Jun 2020 09:04:06: 8000000 INFO @ Tue, 16 Jun 2020 09:04:07: 17000000 INFO @ Tue, 16 Jun 2020 09:04:13: 13000000 INFO @ Tue, 16 Jun 2020 09:04:14: 9000000 INFO @ Tue, 16 Jun 2020 09:04:15: 18000000 INFO @ Tue, 16 Jun 2020 09:04:21: 14000000 INFO @ Tue, 16 Jun 2020 09:04:21: 10000000 INFO @ Tue, 16 Jun 2020 09:04:22: 19000000 INFO @ Tue, 16 Jun 2020 09:04:29: 15000000 INFO @ Tue, 16 Jun 2020 09:04:29: 11000000 INFO @ Tue, 16 Jun 2020 09:04:30: 20000000 INFO @ Tue, 16 Jun 2020 09:04:36: 16000000 INFO @ Tue, 16 Jun 2020 09:04:37: 12000000 INFO @ Tue, 16 Jun 2020 09:04:37: 21000000 INFO @ Tue, 16 Jun 2020 09:04:44: 17000000 INFO @ Tue, 16 Jun 2020 09:04:44: 13000000 INFO @ Tue, 16 Jun 2020 09:04:45: 22000000 INFO @ Tue, 16 Jun 2020 09:04:51: 18000000 INFO @ Tue, 16 Jun 2020 09:04:52: 14000000 INFO @ Tue, 16 Jun 2020 09:04:52: 23000000 INFO @ Tue, 16 Jun 2020 09:04:59: 19000000 INFO @ Tue, 16 Jun 2020 09:04:59: 15000000 INFO @ Tue, 16 Jun 2020 09:05:00: 24000000 INFO @ Tue, 16 Jun 2020 09:05:06: 20000000 INFO @ Tue, 16 Jun 2020 09:05:07: 16000000 INFO @ Tue, 16 Jun 2020 09:05:07: 25000000 INFO @ Tue, 16 Jun 2020 09:05:14: 21000000 INFO @ Tue, 16 Jun 2020 09:05:15: 17000000 INFO @ Tue, 16 Jun 2020 09:05:15: 26000000 INFO @ Tue, 16 Jun 2020 09:05:22: 22000000 INFO @ Tue, 16 Jun 2020 09:05:23: 18000000 INFO @ Tue, 16 Jun 2020 09:05:23: 27000000 INFO @ Tue, 16 Jun 2020 09:05:30: 23000000 INFO @ Tue, 16 Jun 2020 09:05:30: 19000000 INFO @ Tue, 16 Jun 2020 09:05:31: 28000000 INFO @ Tue, 16 Jun 2020 09:05:38: 24000000 INFO @ Tue, 16 Jun 2020 09:05:38: 29000000 INFO @ Tue, 16 Jun 2020 09:05:38: 20000000 INFO @ Tue, 16 Jun 2020 09:05:45: 25000000 INFO @ Tue, 16 Jun 2020 09:05:46: 30000000 INFO @ Tue, 16 Jun 2020 09:05:46: 21000000 INFO @ Tue, 16 Jun 2020 09:05:53: 26000000 INFO @ Tue, 16 Jun 2020 09:05:53: 31000000 INFO @ Tue, 16 Jun 2020 09:05:54: 22000000 INFO @ Tue, 16 Jun 2020 09:06:01: 27000000 INFO @ Tue, 16 Jun 2020 09:06:01: 32000000 INFO @ Tue, 16 Jun 2020 09:06:01: 23000000 INFO @ Tue, 16 Jun 2020 09:06:08: 28000000 INFO @ Tue, 16 Jun 2020 09:06:09: 33000000 INFO @ Tue, 16 Jun 2020 09:06:09: 24000000 INFO @ Tue, 16 Jun 2020 09:06:16: 29000000 INFO @ Tue, 16 Jun 2020 09:06:16: 34000000 INFO @ Tue, 16 Jun 2020 09:06:17: 25000000 INFO @ Tue, 16 Jun 2020 09:06:24: 35000000 INFO @ Tue, 16 Jun 2020 09:06:24: 30000000 INFO @ Tue, 16 Jun 2020 09:06:24: 26000000 INFO @ Tue, 16 Jun 2020 09:06:32: 36000000 INFO @ Tue, 16 Jun 2020 09:06:32: 31000000 INFO @ Tue, 16 Jun 2020 09:06:32: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:06:39: 37000000 INFO @ Tue, 16 Jun 2020 09:06:39: 32000000 INFO @ Tue, 16 Jun 2020 09:06:40: 28000000 INFO @ Tue, 16 Jun 2020 09:06:47: 38000000 INFO @ Tue, 16 Jun 2020 09:06:47: 33000000 INFO @ Tue, 16 Jun 2020 09:06:48: 29000000 INFO @ Tue, 16 Jun 2020 09:06:54: 39000000 INFO @ Tue, 16 Jun 2020 09:06:55: 34000000 INFO @ Tue, 16 Jun 2020 09:06:55: 30000000 INFO @ Tue, 16 Jun 2020 09:07:02: 40000000 INFO @ Tue, 16 Jun 2020 09:07:02: 35000000 INFO @ Tue, 16 Jun 2020 09:07:03: 31000000 INFO @ Tue, 16 Jun 2020 09:07:09: 41000000 INFO @ Tue, 16 Jun 2020 09:07:10: 36000000 INFO @ Tue, 16 Jun 2020 09:07:10: 32000000 INFO @ Tue, 16 Jun 2020 09:07:17: 42000000 INFO @ Tue, 16 Jun 2020 09:07:17: 37000000 INFO @ Tue, 16 Jun 2020 09:07:18: 33000000 INFO @ Tue, 16 Jun 2020 09:07:24: 43000000 INFO @ Tue, 16 Jun 2020 09:07:25: 38000000 INFO @ Tue, 16 Jun 2020 09:07:26: 34000000 INFO @ Tue, 16 Jun 2020 09:07:32: 44000000 INFO @ Tue, 16 Jun 2020 09:07:32: 39000000 INFO @ Tue, 16 Jun 2020 09:07:33: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:07:33: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:07:33: #1 total tags in treatment: 44108422 INFO @ Tue, 16 Jun 2020 09:07:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:07:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:07:33: 35000000 INFO @ Tue, 16 Jun 2020 09:07:34: #1 tags after filtering in treatment: 44108422 INFO @ Tue, 16 Jun 2020 09:07:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:07:34: #1 finished! INFO @ Tue, 16 Jun 2020 09:07:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:07:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:07:36: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:07:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:07:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:07:39: 40000000 INFO @ Tue, 16 Jun 2020 09:07:40: 36000000 INFO @ Tue, 16 Jun 2020 09:07:46: 41000000 INFO @ Tue, 16 Jun 2020 09:07:47: 37000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:07:53: 42000000 INFO @ Tue, 16 Jun 2020 09:07:54: 38000000 INFO @ Tue, 16 Jun 2020 09:07:59: 43000000 INFO @ Tue, 16 Jun 2020 09:08:00: 39000000 INFO @ Tue, 16 Jun 2020 09:08:06: 44000000 INFO @ Tue, 16 Jun 2020 09:08:07: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:08:07: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:08:07: #1 total tags in treatment: 44108422 INFO @ Tue, 16 Jun 2020 09:08:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:08:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:08:07: 40000000 INFO @ Tue, 16 Jun 2020 09:08:07: #1 tags after filtering in treatment: 44108422 INFO @ Tue, 16 Jun 2020 09:08:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:08:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:08:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:08:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:08:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:08:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:08:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:08:13: 41000000 INFO @ Tue, 16 Jun 2020 09:08:20: 42000000 INFO @ Tue, 16 Jun 2020 09:08:26: 43000000 INFO @ Tue, 16 Jun 2020 09:08:32: 44000000 INFO @ Tue, 16 Jun 2020 09:08:33: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:08:33: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:08:33: #1 total tags in treatment: 44108422 INFO @ Tue, 16 Jun 2020 09:08:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:08:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:08:34: #1 tags after filtering in treatment: 44108422 INFO @ Tue, 16 Jun 2020 09:08:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:08:34: #1 finished! INFO @ Tue, 16 Jun 2020 09:08:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:08:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:08:36: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:08:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:08:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973386/SRX2973386.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling