Job ID = 6367176 SRX = SRX2973382 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:23:46 prefetch.2.10.7: 1) Downloading 'SRR5786987'... 2020-06-15T23:23:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:28:43 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:28:43 prefetch.2.10.7: 1) 'SRR5786987' was downloaded successfully 2020-06-15T23:28:43 prefetch.2.10.7: 'SRR5786987' has 0 unresolved dependencies Read 50760011 spots for SRR5786987/SRR5786987.sra Written 50760011 spots for SRR5786987/SRR5786987.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:21 50760011 reads; of these: 50760011 (100.00%) were unpaired; of these: 1385178 (2.73%) aligned 0 times 41652916 (82.06%) aligned exactly 1 time 7721917 (15.21%) aligned >1 times 97.27% overall alignment rate Time searching: 00:18:21 Overall time: 00:18:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9879792 / 49374833 = 0.2001 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:21: 1000000 INFO @ Tue, 16 Jun 2020 09:03:28: 2000000 INFO @ Tue, 16 Jun 2020 09:03:36: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:03:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:03:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:03:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:03:45: 4000000 INFO @ Tue, 16 Jun 2020 09:03:51: 1000000 INFO @ Tue, 16 Jun 2020 09:03:53: 5000000 INFO @ Tue, 16 Jun 2020 09:03:59: 2000000 INFO @ Tue, 16 Jun 2020 09:04:02: 6000000 INFO @ Tue, 16 Jun 2020 09:04:08: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:04:11: 7000000 INFO @ Tue, 16 Jun 2020 09:04:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:04:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:04:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:04:17: 4000000 INFO @ Tue, 16 Jun 2020 09:04:20: 8000000 INFO @ Tue, 16 Jun 2020 09:04:21: 1000000 INFO @ Tue, 16 Jun 2020 09:04:26: 5000000 INFO @ Tue, 16 Jun 2020 09:04:29: 9000000 INFO @ Tue, 16 Jun 2020 09:04:29: 2000000 INFO @ Tue, 16 Jun 2020 09:04:34: 6000000 INFO @ Tue, 16 Jun 2020 09:04:37: 3000000 INFO @ Tue, 16 Jun 2020 09:04:38: 10000000 INFO @ Tue, 16 Jun 2020 09:04:43: 7000000 INFO @ Tue, 16 Jun 2020 09:04:45: 4000000 INFO @ Tue, 16 Jun 2020 09:04:47: 11000000 INFO @ Tue, 16 Jun 2020 09:04:52: 8000000 INFO @ Tue, 16 Jun 2020 09:04:53: 5000000 INFO @ Tue, 16 Jun 2020 09:04:56: 12000000 INFO @ Tue, 16 Jun 2020 09:05:00: 9000000 INFO @ Tue, 16 Jun 2020 09:05:01: 6000000 INFO @ Tue, 16 Jun 2020 09:05:05: 13000000 INFO @ Tue, 16 Jun 2020 09:05:09: 10000000 INFO @ Tue, 16 Jun 2020 09:05:09: 7000000 INFO @ Tue, 16 Jun 2020 09:05:14: 14000000 INFO @ Tue, 16 Jun 2020 09:05:18: 8000000 INFO @ Tue, 16 Jun 2020 09:05:18: 11000000 INFO @ Tue, 16 Jun 2020 09:05:22: 15000000 INFO @ Tue, 16 Jun 2020 09:05:26: 9000000 INFO @ Tue, 16 Jun 2020 09:05:26: 12000000 INFO @ Tue, 16 Jun 2020 09:05:31: 16000000 INFO @ Tue, 16 Jun 2020 09:05:34: 10000000 INFO @ Tue, 16 Jun 2020 09:05:35: 13000000 INFO @ Tue, 16 Jun 2020 09:05:40: 17000000 INFO @ Tue, 16 Jun 2020 09:05:42: 11000000 INFO @ Tue, 16 Jun 2020 09:05:44: 14000000 INFO @ Tue, 16 Jun 2020 09:05:49: 18000000 INFO @ Tue, 16 Jun 2020 09:05:50: 12000000 INFO @ Tue, 16 Jun 2020 09:05:53: 15000000 INFO @ Tue, 16 Jun 2020 09:05:58: 13000000 INFO @ Tue, 16 Jun 2020 09:05:58: 19000000 INFO @ Tue, 16 Jun 2020 09:06:02: 16000000 INFO @ Tue, 16 Jun 2020 09:06:06: 14000000 INFO @ Tue, 16 Jun 2020 09:06:08: 20000000 INFO @ Tue, 16 Jun 2020 09:06:11: 17000000 INFO @ Tue, 16 Jun 2020 09:06:14: 15000000 INFO @ Tue, 16 Jun 2020 09:06:16: 21000000 INFO @ Tue, 16 Jun 2020 09:06:20: 18000000 INFO @ Tue, 16 Jun 2020 09:06:22: 16000000 INFO @ Tue, 16 Jun 2020 09:06:26: 22000000 INFO @ Tue, 16 Jun 2020 09:06:29: 19000000 INFO @ Tue, 16 Jun 2020 09:06:31: 17000000 INFO @ Tue, 16 Jun 2020 09:06:35: 23000000 INFO @ Tue, 16 Jun 2020 09:06:39: 20000000 INFO @ Tue, 16 Jun 2020 09:06:39: 18000000 INFO @ Tue, 16 Jun 2020 09:06:44: 24000000 INFO @ Tue, 16 Jun 2020 09:06:47: 19000000 INFO @ Tue, 16 Jun 2020 09:06:48: 21000000 INFO @ Tue, 16 Jun 2020 09:06:53: 25000000 INFO @ Tue, 16 Jun 2020 09:06:55: 20000000 INFO @ Tue, 16 Jun 2020 09:06:57: 22000000 INFO @ Tue, 16 Jun 2020 09:07:02: 26000000 INFO @ Tue, 16 Jun 2020 09:07:03: 21000000 INFO @ Tue, 16 Jun 2020 09:07:06: 23000000 INFO @ Tue, 16 Jun 2020 09:07:11: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:07:12: 22000000 INFO @ Tue, 16 Jun 2020 09:07:15: 24000000 INFO @ Tue, 16 Jun 2020 09:07:19: 28000000 INFO @ Tue, 16 Jun 2020 09:07:20: 23000000 INFO @ Tue, 16 Jun 2020 09:07:23: 25000000 INFO @ Tue, 16 Jun 2020 09:07:28: 24000000 INFO @ Tue, 16 Jun 2020 09:07:28: 29000000 INFO @ Tue, 16 Jun 2020 09:07:32: 26000000 INFO @ Tue, 16 Jun 2020 09:07:36: 25000000 INFO @ Tue, 16 Jun 2020 09:07:37: 30000000 INFO @ Tue, 16 Jun 2020 09:07:41: 27000000 INFO @ Tue, 16 Jun 2020 09:07:44: 26000000 INFO @ Tue, 16 Jun 2020 09:07:46: 31000000 INFO @ Tue, 16 Jun 2020 09:07:50: 28000000 INFO @ Tue, 16 Jun 2020 09:07:53: 27000000 INFO @ Tue, 16 Jun 2020 09:07:55: 32000000 INFO @ Tue, 16 Jun 2020 09:07:59: 29000000 INFO @ Tue, 16 Jun 2020 09:08:01: 28000000 INFO @ Tue, 16 Jun 2020 09:08:05: 33000000 INFO @ Tue, 16 Jun 2020 09:08:08: 30000000 INFO @ Tue, 16 Jun 2020 09:08:09: 29000000 INFO @ Tue, 16 Jun 2020 09:08:14: 34000000 INFO @ Tue, 16 Jun 2020 09:08:16: 31000000 INFO @ Tue, 16 Jun 2020 09:08:18: 30000000 INFO @ Tue, 16 Jun 2020 09:08:23: 35000000 INFO @ Tue, 16 Jun 2020 09:08:25: 32000000 INFO @ Tue, 16 Jun 2020 09:08:26: 31000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:08:32: 36000000 INFO @ Tue, 16 Jun 2020 09:08:34: 32000000 INFO @ Tue, 16 Jun 2020 09:08:34: 33000000 INFO @ Tue, 16 Jun 2020 09:08:42: 37000000 INFO @ Tue, 16 Jun 2020 09:08:43: 33000000 INFO @ Tue, 16 Jun 2020 09:08:43: 34000000 INFO @ Tue, 16 Jun 2020 09:08:51: 34000000 INFO @ Tue, 16 Jun 2020 09:08:51: 38000000 INFO @ Tue, 16 Jun 2020 09:08:52: 35000000 INFO @ Tue, 16 Jun 2020 09:08:59: 35000000 INFO @ Tue, 16 Jun 2020 09:09:01: 39000000 INFO @ Tue, 16 Jun 2020 09:09:02: 36000000 INFO @ Tue, 16 Jun 2020 09:09:05: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:09:05: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:09:05: #1 total tags in treatment: 39495041 INFO @ Tue, 16 Jun 2020 09:09:05: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:09:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:09:06: #1 tags after filtering in treatment: 39495041 INFO @ Tue, 16 Jun 2020 09:09:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:09:06: #1 finished! INFO @ Tue, 16 Jun 2020 09:09:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:09:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:09:07: 36000000 INFO @ Tue, 16 Jun 2020 09:09:09: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:09:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:09:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:09:11: 37000000 INFO @ Tue, 16 Jun 2020 09:09:16: 37000000 INFO @ Tue, 16 Jun 2020 09:09:20: 38000000 INFO @ Tue, 16 Jun 2020 09:09:24: 38000000 INFO @ Tue, 16 Jun 2020 09:09:29: 39000000 INFO @ Tue, 16 Jun 2020 09:09:32: 39000000 INFO @ Tue, 16 Jun 2020 09:09:34: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:09:34: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:09:34: #1 total tags in treatment: 39495041 INFO @ Tue, 16 Jun 2020 09:09:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:09:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:09:35: #1 tags after filtering in treatment: 39495041 INFO @ Tue, 16 Jun 2020 09:09:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:09:35: #1 finished! INFO @ Tue, 16 Jun 2020 09:09:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:09:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:09:36: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:09:36: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:09:36: #1 total tags in treatment: 39495041 INFO @ Tue, 16 Jun 2020 09:09:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:09:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:09:36: #1 tags after filtering in treatment: 39495041 INFO @ Tue, 16 Jun 2020 09:09:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:09:36: #1 finished! INFO @ Tue, 16 Jun 2020 09:09:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:09:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:09:38: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:09:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:09:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:09:39: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:09:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:09:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973382/SRX2973382.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling