Job ID = 6367175 SRX = SRX2973381 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:26:06 prefetch.2.10.7: 1) Downloading 'SRR5786988'... 2020-06-15T23:26:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:33:35 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:33:35 prefetch.2.10.7: 1) 'SRR5786988' was downloaded successfully 2020-06-15T23:33:35 prefetch.2.10.7: 'SRR5786988' has 0 unresolved dependencies Read 54119838 spots for SRR5786988/SRR5786988.sra Written 54119838 spots for SRR5786988/SRR5786988.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:29 54119838 reads; of these: 54119838 (100.00%) were unpaired; of these: 1596357 (2.95%) aligned 0 times 44662868 (82.53%) aligned exactly 1 time 7860613 (14.52%) aligned >1 times 97.05% overall alignment rate Time searching: 00:18:29 Overall time: 00:18:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13339965 / 52523481 = 0.2540 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:08:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:08:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:08:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:08:27: 1000000 INFO @ Tue, 16 Jun 2020 09:08:34: 2000000 INFO @ Tue, 16 Jun 2020 09:08:41: 3000000 INFO @ Tue, 16 Jun 2020 09:08:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:08:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:08:50: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:08:50: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:08:55: 5000000 INFO @ Tue, 16 Jun 2020 09:08:58: 1000000 INFO @ Tue, 16 Jun 2020 09:09:03: 6000000 INFO @ Tue, 16 Jun 2020 09:09:06: 2000000 INFO @ Tue, 16 Jun 2020 09:09:10: 7000000 INFO @ Tue, 16 Jun 2020 09:09:13: 3000000 INFO @ Tue, 16 Jun 2020 09:09:18: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:09:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:09:20: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:09:20: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:09:21: 4000000 INFO @ Tue, 16 Jun 2020 09:09:26: 9000000 INFO @ Tue, 16 Jun 2020 09:09:29: 1000000 INFO @ Tue, 16 Jun 2020 09:09:29: 5000000 INFO @ Tue, 16 Jun 2020 09:09:34: 10000000 INFO @ Tue, 16 Jun 2020 09:09:37: 2000000 INFO @ Tue, 16 Jun 2020 09:09:37: 6000000 INFO @ Tue, 16 Jun 2020 09:09:42: 11000000 INFO @ Tue, 16 Jun 2020 09:09:45: 3000000 INFO @ Tue, 16 Jun 2020 09:09:45: 7000000 INFO @ Tue, 16 Jun 2020 09:09:51: 12000000 INFO @ Tue, 16 Jun 2020 09:09:53: 8000000 INFO @ Tue, 16 Jun 2020 09:09:53: 4000000 INFO @ Tue, 16 Jun 2020 09:09:59: 13000000 INFO @ Tue, 16 Jun 2020 09:10:01: 9000000 INFO @ Tue, 16 Jun 2020 09:10:01: 5000000 INFO @ Tue, 16 Jun 2020 09:10:07: 14000000 INFO @ Tue, 16 Jun 2020 09:10:09: 10000000 INFO @ Tue, 16 Jun 2020 09:10:09: 6000000 INFO @ Tue, 16 Jun 2020 09:10:15: 15000000 INFO @ Tue, 16 Jun 2020 09:10:17: 11000000 INFO @ Tue, 16 Jun 2020 09:10:17: 7000000 INFO @ Tue, 16 Jun 2020 09:10:23: 16000000 INFO @ Tue, 16 Jun 2020 09:10:25: 12000000 INFO @ Tue, 16 Jun 2020 09:10:25: 8000000 INFO @ Tue, 16 Jun 2020 09:10:31: 17000000 INFO @ Tue, 16 Jun 2020 09:10:33: 13000000 INFO @ Tue, 16 Jun 2020 09:10:33: 9000000 INFO @ Tue, 16 Jun 2020 09:10:38: 18000000 INFO @ Tue, 16 Jun 2020 09:10:41: 14000000 INFO @ Tue, 16 Jun 2020 09:10:41: 10000000 INFO @ Tue, 16 Jun 2020 09:10:46: 19000000 INFO @ Tue, 16 Jun 2020 09:10:49: 15000000 INFO @ Tue, 16 Jun 2020 09:10:49: 11000000 INFO @ Tue, 16 Jun 2020 09:10:54: 20000000 INFO @ Tue, 16 Jun 2020 09:10:56: 16000000 INFO @ Tue, 16 Jun 2020 09:10:57: 12000000 INFO @ Tue, 16 Jun 2020 09:11:02: 21000000 INFO @ Tue, 16 Jun 2020 09:11:04: 17000000 INFO @ Tue, 16 Jun 2020 09:11:05: 13000000 INFO @ Tue, 16 Jun 2020 09:11:10: 22000000 INFO @ Tue, 16 Jun 2020 09:11:12: 18000000 INFO @ Tue, 16 Jun 2020 09:11:13: 14000000 INFO @ Tue, 16 Jun 2020 09:11:18: 23000000 INFO @ Tue, 16 Jun 2020 09:11:20: 19000000 INFO @ Tue, 16 Jun 2020 09:11:21: 15000000 INFO @ Tue, 16 Jun 2020 09:11:25: 24000000 INFO @ Tue, 16 Jun 2020 09:11:28: 20000000 INFO @ Tue, 16 Jun 2020 09:11:28: 16000000 INFO @ Tue, 16 Jun 2020 09:11:33: 25000000 INFO @ Tue, 16 Jun 2020 09:11:35: 21000000 INFO @ Tue, 16 Jun 2020 09:11:36: 17000000 INFO @ Tue, 16 Jun 2020 09:11:41: 26000000 INFO @ Tue, 16 Jun 2020 09:11:43: 22000000 INFO @ Tue, 16 Jun 2020 09:11:44: 18000000 INFO @ Tue, 16 Jun 2020 09:11:49: 27000000 INFO @ Tue, 16 Jun 2020 09:11:51: 23000000 INFO @ Tue, 16 Jun 2020 09:11:52: 19000000 INFO @ Tue, 16 Jun 2020 09:11:56: 28000000 INFO @ Tue, 16 Jun 2020 09:11:59: 24000000 INFO @ Tue, 16 Jun 2020 09:12:00: 20000000 INFO @ Tue, 16 Jun 2020 09:12:04: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:12:07: 25000000 INFO @ Tue, 16 Jun 2020 09:12:07: 21000000 INFO @ Tue, 16 Jun 2020 09:12:12: 30000000 INFO @ Tue, 16 Jun 2020 09:12:14: 26000000 INFO @ Tue, 16 Jun 2020 09:12:15: 22000000 INFO @ Tue, 16 Jun 2020 09:12:19: 31000000 INFO @ Tue, 16 Jun 2020 09:12:22: 27000000 INFO @ Tue, 16 Jun 2020 09:12:23: 23000000 INFO @ Tue, 16 Jun 2020 09:12:27: 32000000 INFO @ Tue, 16 Jun 2020 09:12:30: 28000000 INFO @ Tue, 16 Jun 2020 09:12:31: 24000000 INFO @ Tue, 16 Jun 2020 09:12:35: 33000000 INFO @ Tue, 16 Jun 2020 09:12:38: 29000000 INFO @ Tue, 16 Jun 2020 09:12:39: 25000000 INFO @ Tue, 16 Jun 2020 09:12:43: 34000000 INFO @ Tue, 16 Jun 2020 09:12:46: 30000000 INFO @ Tue, 16 Jun 2020 09:12:47: 26000000 INFO @ Tue, 16 Jun 2020 09:12:51: 35000000 INFO @ Tue, 16 Jun 2020 09:12:54: 31000000 INFO @ Tue, 16 Jun 2020 09:12:55: 27000000 INFO @ Tue, 16 Jun 2020 09:12:58: 36000000 INFO @ Tue, 16 Jun 2020 09:13:02: 32000000 INFO @ Tue, 16 Jun 2020 09:13:03: 28000000 INFO @ Tue, 16 Jun 2020 09:13:06: 37000000 INFO @ Tue, 16 Jun 2020 09:13:10: 33000000 INFO @ Tue, 16 Jun 2020 09:13:11: 29000000 INFO @ Tue, 16 Jun 2020 09:13:14: 38000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:13:18: 34000000 INFO @ Tue, 16 Jun 2020 09:13:19: 30000000 INFO @ Tue, 16 Jun 2020 09:13:22: 39000000 INFO @ Tue, 16 Jun 2020 09:13:23: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:13:23: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:13:23: #1 total tags in treatment: 39183516 INFO @ Tue, 16 Jun 2020 09:13:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:24: #1 tags after filtering in treatment: 39183516 INFO @ Tue, 16 Jun 2020 09:13:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:24: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:13:25: 35000000 INFO @ Tue, 16 Jun 2020 09:13:26: 31000000 INFO @ Tue, 16 Jun 2020 09:13:27: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:13:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:13:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:13:33: 36000000 INFO @ Tue, 16 Jun 2020 09:13:34: 32000000 INFO @ Tue, 16 Jun 2020 09:13:41: 37000000 INFO @ Tue, 16 Jun 2020 09:13:42: 33000000 INFO @ Tue, 16 Jun 2020 09:13:48: 38000000 INFO @ Tue, 16 Jun 2020 09:13:50: 34000000 INFO @ Tue, 16 Jun 2020 09:13:56: 39000000 INFO @ Tue, 16 Jun 2020 09:13:57: 35000000 INFO @ Tue, 16 Jun 2020 09:13:57: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:13:57: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:13:57: #1 total tags in treatment: 39183516 INFO @ Tue, 16 Jun 2020 09:13:57: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:13:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:13:58: #1 tags after filtering in treatment: 39183516 INFO @ Tue, 16 Jun 2020 09:13:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:13:58: #1 finished! INFO @ Tue, 16 Jun 2020 09:13:58: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:13:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:00: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:14:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:14:04: 36000000 INFO @ Tue, 16 Jun 2020 09:14:11: 37000000 INFO @ Tue, 16 Jun 2020 09:14:18: 38000000 INFO @ Tue, 16 Jun 2020 09:14:25: 39000000 INFO @ Tue, 16 Jun 2020 09:14:26: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:14:26: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:14:26: #1 total tags in treatment: 39183516 INFO @ Tue, 16 Jun 2020 09:14:26: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:27: #1 tags after filtering in treatment: 39183516 INFO @ Tue, 16 Jun 2020 09:14:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:27: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:27: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:29: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:14:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:14:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2973381/SRX2973381.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling