Job ID = 6367173 SRX = SRX2965747 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:11:31 prefetch.2.10.7: 1) Downloading 'SRR5766332'... 2020-06-15T23:11:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:16:04 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:16:04 prefetch.2.10.7: 1) 'SRR5766332' was downloaded successfully Read 33011896 spots for SRR5766332/SRR5766332.sra Written 33011896 spots for SRR5766332/SRR5766332.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:21 33011896 reads; of these: 33011896 (100.00%) were unpaired; of these: 3791082 (11.48%) aligned 0 times 24545046 (74.35%) aligned exactly 1 time 4675768 (14.16%) aligned >1 times 88.52% overall alignment rate Time searching: 00:14:22 Overall time: 00:14:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3656959 / 29220814 = 0.1251 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:41:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:41:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:41:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:41:33: 1000000 INFO @ Tue, 16 Jun 2020 08:41:41: 2000000 INFO @ Tue, 16 Jun 2020 08:41:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:41:55: 4000000 INFO @ Tue, 16 Jun 2020 08:41:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:41:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:41:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:42:04: 5000000 INFO @ Tue, 16 Jun 2020 08:42:05: 1000000 INFO @ Tue, 16 Jun 2020 08:42:12: 6000000 INFO @ Tue, 16 Jun 2020 08:42:14: 2000000 INFO @ Tue, 16 Jun 2020 08:42:21: 7000000 INFO @ Tue, 16 Jun 2020 08:42:23: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:42:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:42:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:42:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:42:31: 8000000 INFO @ Tue, 16 Jun 2020 08:42:33: 4000000 INFO @ Tue, 16 Jun 2020 08:42:37: 1000000 INFO @ Tue, 16 Jun 2020 08:42:41: 9000000 INFO @ Tue, 16 Jun 2020 08:42:43: 5000000 INFO @ Tue, 16 Jun 2020 08:42:48: 2000000 INFO @ Tue, 16 Jun 2020 08:42:51: 10000000 INFO @ Tue, 16 Jun 2020 08:42:53: 6000000 INFO @ Tue, 16 Jun 2020 08:42:59: 3000000 INFO @ Tue, 16 Jun 2020 08:43:01: 11000000 INFO @ Tue, 16 Jun 2020 08:43:03: 7000000 INFO @ Tue, 16 Jun 2020 08:43:10: 4000000 INFO @ Tue, 16 Jun 2020 08:43:11: 12000000 INFO @ Tue, 16 Jun 2020 08:43:13: 8000000 INFO @ Tue, 16 Jun 2020 08:43:20: 5000000 INFO @ Tue, 16 Jun 2020 08:43:21: 13000000 INFO @ Tue, 16 Jun 2020 08:43:23: 9000000 INFO @ Tue, 16 Jun 2020 08:43:31: 6000000 INFO @ Tue, 16 Jun 2020 08:43:31: 14000000 INFO @ Tue, 16 Jun 2020 08:43:33: 10000000 INFO @ Tue, 16 Jun 2020 08:43:42: 7000000 INFO @ Tue, 16 Jun 2020 08:43:42: 15000000 INFO @ Tue, 16 Jun 2020 08:43:43: 11000000 INFO @ Tue, 16 Jun 2020 08:43:52: 16000000 INFO @ Tue, 16 Jun 2020 08:43:52: 8000000 INFO @ Tue, 16 Jun 2020 08:43:53: 12000000 INFO @ Tue, 16 Jun 2020 08:44:02: 17000000 INFO @ Tue, 16 Jun 2020 08:44:03: 9000000 INFO @ Tue, 16 Jun 2020 08:44:03: 13000000 INFO @ Tue, 16 Jun 2020 08:44:12: 18000000 INFO @ Tue, 16 Jun 2020 08:44:13: 10000000 INFO @ Tue, 16 Jun 2020 08:44:13: 14000000 INFO @ Tue, 16 Jun 2020 08:44:22: 19000000 INFO @ Tue, 16 Jun 2020 08:44:24: 15000000 INFO @ Tue, 16 Jun 2020 08:44:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:44:32: 20000000 INFO @ Tue, 16 Jun 2020 08:44:34: 16000000 INFO @ Tue, 16 Jun 2020 08:44:35: 12000000 INFO @ Tue, 16 Jun 2020 08:44:43: 21000000 INFO @ Tue, 16 Jun 2020 08:44:44: 17000000 INFO @ Tue, 16 Jun 2020 08:44:45: 13000000 INFO @ Tue, 16 Jun 2020 08:44:53: 22000000 INFO @ Tue, 16 Jun 2020 08:44:54: 18000000 INFO @ Tue, 16 Jun 2020 08:44:56: 14000000 INFO @ Tue, 16 Jun 2020 08:45:03: 23000000 INFO @ Tue, 16 Jun 2020 08:45:04: 19000000 INFO @ Tue, 16 Jun 2020 08:45:06: 15000000 INFO @ Tue, 16 Jun 2020 08:45:13: 24000000 INFO @ Tue, 16 Jun 2020 08:45:14: 20000000 INFO @ Tue, 16 Jun 2020 08:45:17: 16000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:45:23: 25000000 INFO @ Tue, 16 Jun 2020 08:45:24: 21000000 INFO @ Tue, 16 Jun 2020 08:45:27: 17000000 INFO @ Tue, 16 Jun 2020 08:45:29: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 08:45:29: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 08:45:29: #1 total tags in treatment: 25563855 INFO @ Tue, 16 Jun 2020 08:45:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:45:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:45:29: #1 tags after filtering in treatment: 25563855 INFO @ Tue, 16 Jun 2020 08:45:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:45:29: #1 finished! INFO @ Tue, 16 Jun 2020 08:45:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:45:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:45:31: #2 number of paired peaks: 89 WARNING @ Tue, 16 Jun 2020 08:45:31: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:45:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:45:34: 22000000 INFO @ Tue, 16 Jun 2020 08:45:38: 18000000 INFO @ Tue, 16 Jun 2020 08:45:43: 23000000 INFO @ Tue, 16 Jun 2020 08:45:49: 19000000 INFO @ Tue, 16 Jun 2020 08:45:53: 24000000 INFO @ Tue, 16 Jun 2020 08:45:59: 20000000 INFO @ Tue, 16 Jun 2020 08:46:03: 25000000 INFO @ Tue, 16 Jun 2020 08:46:08: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 08:46:08: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 08:46:08: #1 total tags in treatment: 25563855 INFO @ Tue, 16 Jun 2020 08:46:08: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:46:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:46:08: #1 tags after filtering in treatment: 25563855 INFO @ Tue, 16 Jun 2020 08:46:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:46:08: #1 finished! INFO @ Tue, 16 Jun 2020 08:46:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:46:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:46:09: 21000000 INFO @ Tue, 16 Jun 2020 08:46:10: #2 number of paired peaks: 89 WARNING @ Tue, 16 Jun 2020 08:46:10: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:46:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:46:18: 22000000 INFO @ Tue, 16 Jun 2020 08:46:27: 23000000 INFO @ Tue, 16 Jun 2020 08:46:36: 24000000 INFO @ Tue, 16 Jun 2020 08:46:46: 25000000 INFO @ Tue, 16 Jun 2020 08:46:52: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 08:46:52: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 08:46:52: #1 total tags in treatment: 25563855 INFO @ Tue, 16 Jun 2020 08:46:52: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:46:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:46:52: #1 tags after filtering in treatment: 25563855 INFO @ Tue, 16 Jun 2020 08:46:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:46:52: #1 finished! INFO @ Tue, 16 Jun 2020 08:46:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:46:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:46:54: #2 number of paired peaks: 89 WARNING @ Tue, 16 Jun 2020 08:46:54: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:46:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965747/SRX2965747.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling