Job ID = 6367172 SRX = SRX2965746 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:24:16 prefetch.2.10.7: 1) Downloading 'SRR5766331'... 2020-06-15T23:24:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:30:16 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:30:16 prefetch.2.10.7: 1) 'SRR5766331' was downloaded successfully Read 35206966 spots for SRR5766331/SRR5766331.sra Written 35206966 spots for SRR5766331/SRR5766331.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:30 35206966 reads; of these: 35206966 (100.00%) were unpaired; of these: 2311398 (6.57%) aligned 0 times 27522311 (78.17%) aligned exactly 1 time 5373257 (15.26%) aligned >1 times 93.43% overall alignment rate Time searching: 00:16:30 Overall time: 00:16:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5963641 / 32895568 = 0.1813 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:59:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:59:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:59:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:59:55: 1000000 INFO @ Tue, 16 Jun 2020 09:00:05: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:00:14: 3000000 INFO @ Tue, 16 Jun 2020 09:00:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:00:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:00:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:00:24: 4000000 INFO @ Tue, 16 Jun 2020 09:00:27: 1000000 INFO @ Tue, 16 Jun 2020 09:00:34: 5000000 INFO @ Tue, 16 Jun 2020 09:00:37: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:00:44: 6000000 INFO @ Tue, 16 Jun 2020 09:00:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:00:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:00:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:00:48: 3000000 INFO @ Tue, 16 Jun 2020 09:00:55: 7000000 INFO @ Tue, 16 Jun 2020 09:00:56: 1000000 INFO @ Tue, 16 Jun 2020 09:00:58: 4000000 INFO @ Tue, 16 Jun 2020 09:01:05: 8000000 INFO @ Tue, 16 Jun 2020 09:01:07: 2000000 INFO @ Tue, 16 Jun 2020 09:01:08: 5000000 INFO @ Tue, 16 Jun 2020 09:01:15: 9000000 INFO @ Tue, 16 Jun 2020 09:01:17: 3000000 INFO @ Tue, 16 Jun 2020 09:01:18: 6000000 INFO @ Tue, 16 Jun 2020 09:01:26: 10000000 INFO @ Tue, 16 Jun 2020 09:01:27: 4000000 INFO @ Tue, 16 Jun 2020 09:01:29: 7000000 INFO @ Tue, 16 Jun 2020 09:01:37: 11000000 INFO @ Tue, 16 Jun 2020 09:01:37: 5000000 INFO @ Tue, 16 Jun 2020 09:01:39: 8000000 INFO @ Tue, 16 Jun 2020 09:01:47: 6000000 INFO @ Tue, 16 Jun 2020 09:01:47: 12000000 INFO @ Tue, 16 Jun 2020 09:01:49: 9000000 INFO @ Tue, 16 Jun 2020 09:01:57: 7000000 INFO @ Tue, 16 Jun 2020 09:01:58: 13000000 INFO @ Tue, 16 Jun 2020 09:02:00: 10000000 INFO @ Tue, 16 Jun 2020 09:02:07: 8000000 INFO @ Tue, 16 Jun 2020 09:02:08: 14000000 INFO @ Tue, 16 Jun 2020 09:02:10: 11000000 INFO @ Tue, 16 Jun 2020 09:02:17: 9000000 INFO @ Tue, 16 Jun 2020 09:02:18: 15000000 INFO @ Tue, 16 Jun 2020 09:02:20: 12000000 INFO @ Tue, 16 Jun 2020 09:02:28: 10000000 INFO @ Tue, 16 Jun 2020 09:02:28: 16000000 INFO @ Tue, 16 Jun 2020 09:02:31: 13000000 INFO @ Tue, 16 Jun 2020 09:02:38: 11000000 INFO @ Tue, 16 Jun 2020 09:02:38: 17000000 INFO @ Tue, 16 Jun 2020 09:02:41: 14000000 INFO @ Tue, 16 Jun 2020 09:02:49: 12000000 INFO @ Tue, 16 Jun 2020 09:02:49: 18000000 INFO @ Tue, 16 Jun 2020 09:02:51: 15000000 INFO @ Tue, 16 Jun 2020 09:02:59: 13000000 INFO @ Tue, 16 Jun 2020 09:02:59: 19000000 INFO @ Tue, 16 Jun 2020 09:03:01: 16000000 INFO @ Tue, 16 Jun 2020 09:03:09: 14000000 INFO @ Tue, 16 Jun 2020 09:03:10: 20000000 INFO @ Tue, 16 Jun 2020 09:03:12: 17000000 INFO @ Tue, 16 Jun 2020 09:03:20: 15000000 INFO @ Tue, 16 Jun 2020 09:03:20: 21000000 INFO @ Tue, 16 Jun 2020 09:03:22: 18000000 INFO @ Tue, 16 Jun 2020 09:03:30: 16000000 INFO @ Tue, 16 Jun 2020 09:03:30: 22000000 INFO @ Tue, 16 Jun 2020 09:03:33: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:03:40: 17000000 INFO @ Tue, 16 Jun 2020 09:03:41: 23000000 INFO @ Tue, 16 Jun 2020 09:03:43: 20000000 INFO @ Tue, 16 Jun 2020 09:03:51: 18000000 INFO @ Tue, 16 Jun 2020 09:03:51: 24000000 INFO @ Tue, 16 Jun 2020 09:03:53: 21000000 INFO @ Tue, 16 Jun 2020 09:04:01: 25000000 INFO @ Tue, 16 Jun 2020 09:04:01: 19000000 INFO @ Tue, 16 Jun 2020 09:04:03: 22000000 INFO @ Tue, 16 Jun 2020 09:04:10: 26000000 INFO @ Tue, 16 Jun 2020 09:04:12: 20000000 INFO @ Tue, 16 Jun 2020 09:04:13: 23000000 INFO @ Tue, 16 Jun 2020 09:04:20: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:04:20: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:04:20: #1 total tags in treatment: 26931927 INFO @ Tue, 16 Jun 2020 09:04:20: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:04:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:04:20: #1 tags after filtering in treatment: 26931927 INFO @ Tue, 16 Jun 2020 09:04:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:04:20: #1 finished! INFO @ Tue, 16 Jun 2020 09:04:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:04:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:04:22: #2 number of paired peaks: 87 WARNING @ Tue, 16 Jun 2020 09:04:22: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:04:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:04:22: 21000000 INFO @ Tue, 16 Jun 2020 09:04:23: 24000000 INFO @ Tue, 16 Jun 2020 09:04:33: 22000000 INFO @ Tue, 16 Jun 2020 09:04:33: 25000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:04:42: 26000000 INFO @ Tue, 16 Jun 2020 09:04:43: 23000000 INFO @ Tue, 16 Jun 2020 09:04:51: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:04:51: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:04:51: #1 total tags in treatment: 26931927 INFO @ Tue, 16 Jun 2020 09:04:51: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:04:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:04:52: #1 tags after filtering in treatment: 26931927 INFO @ Tue, 16 Jun 2020 09:04:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:04:52: #1 finished! INFO @ Tue, 16 Jun 2020 09:04:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:04:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:04:53: 24000000 INFO @ Tue, 16 Jun 2020 09:04:54: #2 number of paired peaks: 87 WARNING @ Tue, 16 Jun 2020 09:04:54: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:04:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:05:02: 25000000 INFO @ Tue, 16 Jun 2020 09:05:11: 26000000 INFO @ Tue, 16 Jun 2020 09:05:19: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:05:19: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:05:19: #1 total tags in treatment: 26931927 INFO @ Tue, 16 Jun 2020 09:05:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:05:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:05:20: #1 tags after filtering in treatment: 26931927 INFO @ Tue, 16 Jun 2020 09:05:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:05:20: #1 finished! INFO @ Tue, 16 Jun 2020 09:05:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:05:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:05:22: #2 number of paired peaks: 87 WARNING @ Tue, 16 Jun 2020 09:05:22: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:05:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965746/SRX2965746.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling