Job ID = 6367166 SRX = SRX2965740 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:17:31 prefetch.2.10.7: 1) Downloading 'SRR5766325'... 2020-06-15T23:17:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:21:57 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:21:57 prefetch.2.10.7: 1) 'SRR5766325' was downloaded successfully 2020-06-15T23:21:57 prefetch.2.10.7: 'SRR5766325' has 0 unresolved dependencies Read 50760011 spots for SRR5766325/SRR5766325.sra Written 50760011 spots for SRR5766325/SRR5766325.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:34 50760011 reads; of these: 50760011 (100.00%) were unpaired; of these: 1385326 (2.73%) aligned 0 times 41652340 (82.06%) aligned exactly 1 time 7722345 (15.21%) aligned >1 times 97.27% overall alignment rate Time searching: 00:17:34 Overall time: 00:17:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9879946 / 49374685 = 0.2001 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:55:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:55:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:55:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:55:08: 1000000 INFO @ Tue, 16 Jun 2020 08:55:14: 2000000 INFO @ Tue, 16 Jun 2020 08:55:20: 3000000 INFO @ Tue, 16 Jun 2020 08:55:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:55:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:55:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:55:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:55:33: 5000000 INFO @ Tue, 16 Jun 2020 08:55:38: 1000000 INFO @ Tue, 16 Jun 2020 08:55:39: 6000000 INFO @ Tue, 16 Jun 2020 08:55:45: 2000000 INFO @ Tue, 16 Jun 2020 08:55:46: 7000000 INFO @ Tue, 16 Jun 2020 08:55:52: 3000000 INFO @ Tue, 16 Jun 2020 08:55:53: 8000000 INFO @ Tue, 16 Jun 2020 08:55:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:56:00: 9000000 INFO @ Tue, 16 Jun 2020 08:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:56:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:56:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:56:07: 5000000 INFO @ Tue, 16 Jun 2020 08:56:08: 10000000 INFO @ Tue, 16 Jun 2020 08:56:10: 1000000 INFO @ Tue, 16 Jun 2020 08:56:14: 6000000 INFO @ Tue, 16 Jun 2020 08:56:15: 11000000 INFO @ Tue, 16 Jun 2020 08:56:19: 2000000 INFO @ Tue, 16 Jun 2020 08:56:22: 7000000 INFO @ Tue, 16 Jun 2020 08:56:23: 12000000 INFO @ Tue, 16 Jun 2020 08:56:27: 3000000 INFO @ Tue, 16 Jun 2020 08:56:30: 8000000 INFO @ Tue, 16 Jun 2020 08:56:31: 13000000 INFO @ Tue, 16 Jun 2020 08:56:35: 4000000 INFO @ Tue, 16 Jun 2020 08:56:38: 9000000 INFO @ Tue, 16 Jun 2020 08:56:39: 14000000 INFO @ Tue, 16 Jun 2020 08:56:44: 5000000 INFO @ Tue, 16 Jun 2020 08:56:46: 10000000 INFO @ Tue, 16 Jun 2020 08:56:47: 15000000 INFO @ Tue, 16 Jun 2020 08:56:52: 6000000 INFO @ Tue, 16 Jun 2020 08:56:53: 11000000 INFO @ Tue, 16 Jun 2020 08:56:54: 16000000 INFO @ Tue, 16 Jun 2020 08:57:00: 7000000 INFO @ Tue, 16 Jun 2020 08:57:01: 12000000 INFO @ Tue, 16 Jun 2020 08:57:02: 17000000 INFO @ Tue, 16 Jun 2020 08:57:08: 8000000 INFO @ Tue, 16 Jun 2020 08:57:09: 13000000 INFO @ Tue, 16 Jun 2020 08:57:10: 18000000 INFO @ Tue, 16 Jun 2020 08:57:16: 9000000 INFO @ Tue, 16 Jun 2020 08:57:17: 14000000 INFO @ Tue, 16 Jun 2020 08:57:18: 19000000 INFO @ Tue, 16 Jun 2020 08:57:24: 10000000 INFO @ Tue, 16 Jun 2020 08:57:24: 15000000 INFO @ Tue, 16 Jun 2020 08:57:25: 20000000 INFO @ Tue, 16 Jun 2020 08:57:32: 16000000 INFO @ Tue, 16 Jun 2020 08:57:32: 11000000 INFO @ Tue, 16 Jun 2020 08:57:33: 21000000 INFO @ Tue, 16 Jun 2020 08:57:40: 17000000 INFO @ Tue, 16 Jun 2020 08:57:41: 12000000 INFO @ Tue, 16 Jun 2020 08:57:41: 22000000 INFO @ Tue, 16 Jun 2020 08:57:48: 18000000 INFO @ Tue, 16 Jun 2020 08:57:48: 23000000 INFO @ Tue, 16 Jun 2020 08:57:49: 13000000 INFO @ Tue, 16 Jun 2020 08:57:56: 19000000 INFO @ Tue, 16 Jun 2020 08:57:56: 24000000 INFO @ Tue, 16 Jun 2020 08:57:57: 14000000 INFO @ Tue, 16 Jun 2020 08:58:03: 20000000 INFO @ Tue, 16 Jun 2020 08:58:04: 25000000 INFO @ Tue, 16 Jun 2020 08:58:05: 15000000 INFO @ Tue, 16 Jun 2020 08:58:11: 21000000 INFO @ Tue, 16 Jun 2020 08:58:12: 26000000 INFO @ Tue, 16 Jun 2020 08:58:13: 16000000 INFO @ Tue, 16 Jun 2020 08:58:18: 22000000 INFO @ Tue, 16 Jun 2020 08:58:19: 27000000 INFO @ Tue, 16 Jun 2020 08:58:21: 17000000 INFO @ Tue, 16 Jun 2020 08:58:26: 23000000 INFO @ Tue, 16 Jun 2020 08:58:27: 28000000 INFO @ Tue, 16 Jun 2020 08:58:29: 18000000 INFO @ Tue, 16 Jun 2020 08:58:34: 24000000 INFO @ Tue, 16 Jun 2020 08:58:35: 29000000 INFO @ Tue, 16 Jun 2020 08:58:37: 19000000 INFO @ Tue, 16 Jun 2020 08:58:41: 25000000 INFO @ Tue, 16 Jun 2020 08:58:42: 30000000 INFO @ Tue, 16 Jun 2020 08:58:45: 20000000 INFO @ Tue, 16 Jun 2020 08:58:49: 26000000 INFO @ Tue, 16 Jun 2020 08:58:50: 31000000 INFO @ Tue, 16 Jun 2020 08:58:53: 21000000 INFO @ Tue, 16 Jun 2020 08:58:57: 27000000 INFO @ Tue, 16 Jun 2020 08:58:58: 32000000 INFO @ Tue, 16 Jun 2020 08:59:01: 22000000 INFO @ Tue, 16 Jun 2020 08:59:04: 28000000 INFO @ Tue, 16 Jun 2020 08:59:05: 33000000 INFO @ Tue, 16 Jun 2020 08:59:10: 23000000 INFO @ Tue, 16 Jun 2020 08:59:12: 29000000 INFO @ Tue, 16 Jun 2020 08:59:13: 34000000 INFO @ Tue, 16 Jun 2020 08:59:18: 24000000 INFO @ Tue, 16 Jun 2020 08:59:20: 30000000 INFO @ Tue, 16 Jun 2020 08:59:20: 35000000 INFO @ Tue, 16 Jun 2020 08:59:26: 25000000 INFO @ Tue, 16 Jun 2020 08:59:27: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:59:28: 36000000 INFO @ Tue, 16 Jun 2020 08:59:34: 26000000 INFO @ Tue, 16 Jun 2020 08:59:35: 32000000 INFO @ Tue, 16 Jun 2020 08:59:35: 37000000 INFO @ Tue, 16 Jun 2020 08:59:42: 33000000 INFO @ Tue, 16 Jun 2020 08:59:43: 27000000 INFO @ Tue, 16 Jun 2020 08:59:43: 38000000 INFO @ Tue, 16 Jun 2020 08:59:50: 34000000 INFO @ Tue, 16 Jun 2020 08:59:50: 39000000 INFO @ Tue, 16 Jun 2020 08:59:51: 28000000 INFO @ Tue, 16 Jun 2020 08:59:54: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 08:59:54: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 08:59:54: #1 total tags in treatment: 39494739 INFO @ Tue, 16 Jun 2020 08:59:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:59:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:59:55: #1 tags after filtering in treatment: 39494739 INFO @ Tue, 16 Jun 2020 08:59:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:59:55: #1 finished! INFO @ Tue, 16 Jun 2020 08:59:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:59:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:59:57: 35000000 INFO @ Tue, 16 Jun 2020 08:59:57: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:59:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:59:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:59:59: 29000000 INFO @ Tue, 16 Jun 2020 09:00:04: 36000000 INFO @ Tue, 16 Jun 2020 09:00:07: 30000000 INFO @ Tue, 16 Jun 2020 09:00:11: 37000000 INFO @ Tue, 16 Jun 2020 09:00:15: 31000000 INFO @ Tue, 16 Jun 2020 09:00:18: 38000000 INFO @ Tue, 16 Jun 2020 09:00:23: 32000000 INFO @ Tue, 16 Jun 2020 09:00:25: 39000000 INFO @ Tue, 16 Jun 2020 09:00:29: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:00:29: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:00:29: #1 total tags in treatment: 39494739 INFO @ Tue, 16 Jun 2020 09:00:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:00:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:00:30: #1 tags after filtering in treatment: 39494739 INFO @ Tue, 16 Jun 2020 09:00:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:00:30: #1 finished! INFO @ Tue, 16 Jun 2020 09:00:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:00:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:00:31: 33000000 INFO @ Tue, 16 Jun 2020 09:00:32: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:00:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:00:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:00:38: 34000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:00:46: 35000000 INFO @ Tue, 16 Jun 2020 09:00:53: 36000000 INFO @ Tue, 16 Jun 2020 09:01:01: 37000000 INFO @ Tue, 16 Jun 2020 09:01:08: 38000000 INFO @ Tue, 16 Jun 2020 09:01:16: 39000000 INFO @ Tue, 16 Jun 2020 09:01:19: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:01:19: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:01:19: #1 total tags in treatment: 39494739 INFO @ Tue, 16 Jun 2020 09:01:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:01:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:01:20: #1 tags after filtering in treatment: 39494739 INFO @ Tue, 16 Jun 2020 09:01:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:01:20: #1 finished! INFO @ Tue, 16 Jun 2020 09:01:20: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:01:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:01:23: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:01:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:01:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965740/SRX2965740.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling