Job ID = 6367164 SRX = SRX2965738 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:35:50 prefetch.2.10.7: 1) Downloading 'SRR5766323'... 2020-06-15T23:35:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:45:28 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:45:28 prefetch.2.10.7: 1) 'SRR5766323' was downloaded successfully 2020-06-15T23:45:28 prefetch.2.10.7: 'SRR5766323' has 0 unresolved dependencies Read 81489952 spots for SRR5766323/SRR5766323.sra Written 81489952 spots for SRR5766323/SRR5766323.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:49 81489952 reads; of these: 81489952 (100.00%) were unpaired; of these: 3649164 (4.48%) aligned 0 times 64462508 (79.10%) aligned exactly 1 time 13378280 (16.42%) aligned >1 times 95.52% overall alignment rate Time searching: 00:28:49 Overall time: 00:28:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 21552383 / 77840788 = 0.2769 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:40:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:40:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:40:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:40:23: 1000000 INFO @ Tue, 16 Jun 2020 09:40:29: 2000000 INFO @ Tue, 16 Jun 2020 09:40:35: 3000000 INFO @ Tue, 16 Jun 2020 09:40:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:40:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:40:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:40:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:40:47: 5000000 INFO @ Tue, 16 Jun 2020 09:40:54: 1000000 INFO @ Tue, 16 Jun 2020 09:40:54: 6000000 INFO @ Tue, 16 Jun 2020 09:41:00: 2000000 INFO @ Tue, 16 Jun 2020 09:41:01: 7000000 INFO @ Tue, 16 Jun 2020 09:41:07: 3000000 INFO @ Tue, 16 Jun 2020 09:41:08: 8000000 INFO @ Tue, 16 Jun 2020 09:41:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:41:15: 9000000 INFO @ Tue, 16 Jun 2020 09:41:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:41:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:41:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:41:21: 5000000 INFO @ Tue, 16 Jun 2020 09:41:22: 10000000 INFO @ Tue, 16 Jun 2020 09:41:23: 1000000 INFO @ Tue, 16 Jun 2020 09:41:28: 6000000 INFO @ Tue, 16 Jun 2020 09:41:29: 11000000 INFO @ Tue, 16 Jun 2020 09:41:29: 2000000 INFO @ Tue, 16 Jun 2020 09:41:35: 3000000 INFO @ Tue, 16 Jun 2020 09:41:36: 7000000 INFO @ Tue, 16 Jun 2020 09:41:36: 12000000 INFO @ Tue, 16 Jun 2020 09:41:42: 4000000 INFO @ Tue, 16 Jun 2020 09:41:43: 8000000 INFO @ Tue, 16 Jun 2020 09:41:43: 13000000 INFO @ Tue, 16 Jun 2020 09:41:48: 5000000 INFO @ Tue, 16 Jun 2020 09:41:50: 9000000 INFO @ Tue, 16 Jun 2020 09:41:50: 14000000 INFO @ Tue, 16 Jun 2020 09:41:54: 6000000 INFO @ Tue, 16 Jun 2020 09:41:57: 10000000 INFO @ Tue, 16 Jun 2020 09:41:57: 15000000 INFO @ Tue, 16 Jun 2020 09:42:00: 7000000 INFO @ Tue, 16 Jun 2020 09:42:05: 11000000 INFO @ Tue, 16 Jun 2020 09:42:05: 16000000 INFO @ Tue, 16 Jun 2020 09:42:07: 8000000 INFO @ Tue, 16 Jun 2020 09:42:12: 12000000 INFO @ Tue, 16 Jun 2020 09:42:12: 17000000 INFO @ Tue, 16 Jun 2020 09:42:13: 9000000 INFO @ Tue, 16 Jun 2020 09:42:19: 13000000 INFO @ Tue, 16 Jun 2020 09:42:19: 18000000 INFO @ Tue, 16 Jun 2020 09:42:19: 10000000 INFO @ Tue, 16 Jun 2020 09:42:26: 11000000 INFO @ Tue, 16 Jun 2020 09:42:26: 14000000 INFO @ Tue, 16 Jun 2020 09:42:27: 19000000 INFO @ Tue, 16 Jun 2020 09:42:32: 12000000 INFO @ Tue, 16 Jun 2020 09:42:34: 15000000 INFO @ Tue, 16 Jun 2020 09:42:34: 20000000 INFO @ Tue, 16 Jun 2020 09:42:38: 13000000 INFO @ Tue, 16 Jun 2020 09:42:41: 16000000 INFO @ Tue, 16 Jun 2020 09:42:41: 21000000 INFO @ Tue, 16 Jun 2020 09:42:44: 14000000 INFO @ Tue, 16 Jun 2020 09:42:48: 17000000 INFO @ Tue, 16 Jun 2020 09:42:48: 22000000 INFO @ Tue, 16 Jun 2020 09:42:51: 15000000 INFO @ Tue, 16 Jun 2020 09:42:55: 18000000 INFO @ Tue, 16 Jun 2020 09:42:55: 23000000 INFO @ Tue, 16 Jun 2020 09:42:57: 16000000 INFO @ Tue, 16 Jun 2020 09:43:03: 19000000 INFO @ Tue, 16 Jun 2020 09:43:03: 24000000 INFO @ Tue, 16 Jun 2020 09:43:03: 17000000 INFO @ Tue, 16 Jun 2020 09:43:09: 18000000 INFO @ Tue, 16 Jun 2020 09:43:10: 20000000 INFO @ Tue, 16 Jun 2020 09:43:10: 25000000 INFO @ Tue, 16 Jun 2020 09:43:15: 19000000 INFO @ Tue, 16 Jun 2020 09:43:17: 21000000 INFO @ Tue, 16 Jun 2020 09:43:17: 26000000 INFO @ Tue, 16 Jun 2020 09:43:22: 20000000 INFO @ Tue, 16 Jun 2020 09:43:25: 22000000 INFO @ Tue, 16 Jun 2020 09:43:25: 27000000 INFO @ Tue, 16 Jun 2020 09:43:29: 21000000 INFO @ Tue, 16 Jun 2020 09:43:32: 23000000 INFO @ Tue, 16 Jun 2020 09:43:32: 28000000 INFO @ Tue, 16 Jun 2020 09:43:36: 22000000 INFO @ Tue, 16 Jun 2020 09:43:39: 24000000 INFO @ Tue, 16 Jun 2020 09:43:39: 29000000 INFO @ Tue, 16 Jun 2020 09:43:44: 23000000 INFO @ Tue, 16 Jun 2020 09:43:46: 30000000 INFO @ Tue, 16 Jun 2020 09:43:46: 25000000 INFO @ Tue, 16 Jun 2020 09:43:51: 24000000 INFO @ Tue, 16 Jun 2020 09:43:53: 31000000 INFO @ Tue, 16 Jun 2020 09:43:53: 26000000 INFO @ Tue, 16 Jun 2020 09:44:00: 32000000 INFO @ Tue, 16 Jun 2020 09:44:00: 25000000 INFO @ Tue, 16 Jun 2020 09:44:00: 27000000 INFO @ Tue, 16 Jun 2020 09:44:07: 33000000 INFO @ Tue, 16 Jun 2020 09:44:07: 28000000 INFO @ Tue, 16 Jun 2020 09:44:08: 26000000 INFO @ Tue, 16 Jun 2020 09:44:14: 34000000 INFO @ Tue, 16 Jun 2020 09:44:14: 29000000 INFO @ Tue, 16 Jun 2020 09:44:17: 27000000 INFO @ Tue, 16 Jun 2020 09:44:21: 35000000 INFO @ Tue, 16 Jun 2020 09:44:21: 30000000 INFO @ Tue, 16 Jun 2020 09:44:25: 28000000 INFO @ Tue, 16 Jun 2020 09:44:28: 36000000 INFO @ Tue, 16 Jun 2020 09:44:29: 31000000 INFO @ Tue, 16 Jun 2020 09:44:34: 29000000 INFO @ Tue, 16 Jun 2020 09:44:36: 37000000 INFO @ Tue, 16 Jun 2020 09:44:36: 32000000 INFO @ Tue, 16 Jun 2020 09:44:42: 30000000 INFO @ Tue, 16 Jun 2020 09:44:44: 38000000 INFO @ Tue, 16 Jun 2020 09:44:44: 33000000 INFO @ Tue, 16 Jun 2020 09:44:51: 31000000 INFO @ Tue, 16 Jun 2020 09:44:51: 39000000 INFO @ Tue, 16 Jun 2020 09:44:51: 34000000 INFO @ Tue, 16 Jun 2020 09:44:59: 40000000 INFO @ Tue, 16 Jun 2020 09:44:59: 35000000 INFO @ Tue, 16 Jun 2020 09:44:59: 32000000 INFO @ Tue, 16 Jun 2020 09:45:06: 41000000 INFO @ Tue, 16 Jun 2020 09:45:07: 36000000 INFO @ Tue, 16 Jun 2020 09:45:08: 33000000 INFO @ Tue, 16 Jun 2020 09:45:14: 42000000 INFO @ Tue, 16 Jun 2020 09:45:14: 37000000 INFO @ Tue, 16 Jun 2020 09:45:17: 34000000 INFO @ Tue, 16 Jun 2020 09:45:21: 43000000 INFO @ Tue, 16 Jun 2020 09:45:22: 38000000 INFO @ Tue, 16 Jun 2020 09:45:25: 35000000 INFO @ Tue, 16 Jun 2020 09:45:29: 44000000 INFO @ Tue, 16 Jun 2020 09:45:30: 39000000 INFO @ Tue, 16 Jun 2020 09:45:34: 36000000 INFO @ Tue, 16 Jun 2020 09:45:37: 45000000 INFO @ Tue, 16 Jun 2020 09:45:38: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:45:43: 37000000 INFO @ Tue, 16 Jun 2020 09:45:45: 46000000 INFO @ Tue, 16 Jun 2020 09:45:46: 41000000 INFO @ Tue, 16 Jun 2020 09:45:51: 38000000 INFO @ Tue, 16 Jun 2020 09:45:53: 47000000 INFO @ Tue, 16 Jun 2020 09:45:54: 42000000 INFO @ Tue, 16 Jun 2020 09:45:59: 39000000 INFO @ Tue, 16 Jun 2020 09:46:01: 48000000 INFO @ Tue, 16 Jun 2020 09:46:02: 43000000 INFO @ Tue, 16 Jun 2020 09:46:06: 40000000 INFO @ Tue, 16 Jun 2020 09:46:09: 49000000 INFO @ Tue, 16 Jun 2020 09:46:09: 44000000 INFO @ Tue, 16 Jun 2020 09:46:14: 41000000 INFO @ Tue, 16 Jun 2020 09:46:17: 50000000 INFO @ Tue, 16 Jun 2020 09:46:17: 45000000 INFO @ Tue, 16 Jun 2020 09:46:21: 42000000 INFO @ Tue, 16 Jun 2020 09:46:24: 51000000 INFO @ Tue, 16 Jun 2020 09:46:25: 46000000 INFO @ Tue, 16 Jun 2020 09:46:28: 43000000 INFO @ Tue, 16 Jun 2020 09:46:32: 52000000 INFO @ Tue, 16 Jun 2020 09:46:33: 47000000 INFO @ Tue, 16 Jun 2020 09:46:34: 44000000 INFO @ Tue, 16 Jun 2020 09:46:40: 53000000 INFO @ Tue, 16 Jun 2020 09:46:41: 48000000 INFO @ Tue, 16 Jun 2020 09:46:41: 45000000 INFO @ Tue, 16 Jun 2020 09:46:47: 46000000 INFO @ Tue, 16 Jun 2020 09:46:48: 54000000 INFO @ Tue, 16 Jun 2020 09:46:49: 49000000 INFO @ Tue, 16 Jun 2020 09:46:54: 47000000 INFO @ Tue, 16 Jun 2020 09:46:55: 55000000 INFO @ Tue, 16 Jun 2020 09:46:56: 50000000 INFO @ Tue, 16 Jun 2020 09:47:00: 48000000 INFO @ Tue, 16 Jun 2020 09:47:03: 56000000 INFO @ Tue, 16 Jun 2020 09:47:04: 51000000 INFO @ Tue, 16 Jun 2020 09:47:06: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:47:06: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:47:06: #1 total tags in treatment: 56288405 INFO @ Tue, 16 Jun 2020 09:47:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:07: #1 tags after filtering in treatment: 56288405 INFO @ Tue, 16 Jun 2020 09:47:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:07: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:07: 49000000 INFO @ Tue, 16 Jun 2020 09:47:10: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:47:11: 52000000 INFO @ Tue, 16 Jun 2020 09:47:13: 50000000 INFO @ Tue, 16 Jun 2020 09:47:18: 53000000 INFO @ Tue, 16 Jun 2020 09:47:20: 51000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:47:25: 54000000 INFO @ Tue, 16 Jun 2020 09:47:27: 52000000 INFO @ Tue, 16 Jun 2020 09:47:31: 55000000 INFO @ Tue, 16 Jun 2020 09:47:33: 53000000 INFO @ Tue, 16 Jun 2020 09:47:38: 56000000 INFO @ Tue, 16 Jun 2020 09:47:40: 54000000 INFO @ Tue, 16 Jun 2020 09:47:40: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:47:40: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:47:40: #1 total tags in treatment: 56288405 INFO @ Tue, 16 Jun 2020 09:47:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:41: #1 tags after filtering in treatment: 56288405 INFO @ Tue, 16 Jun 2020 09:47:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:41: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:41: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:45: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:47:46: 55000000 INFO @ Tue, 16 Jun 2020 09:47:53: 56000000 INFO @ Tue, 16 Jun 2020 09:47:55: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:47:55: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:47:55: #1 total tags in treatment: 56288405 INFO @ Tue, 16 Jun 2020 09:47:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:47:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:47:56: #1 tags after filtering in treatment: 56288405 INFO @ Tue, 16 Jun 2020 09:47:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:47:56: #1 finished! INFO @ Tue, 16 Jun 2020 09:47:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:47:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:47:59: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:47:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:47:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965738/SRX2965738.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling