Job ID = 6367159 SRX = SRX2965733 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:19:20 prefetch.2.10.7: 1) Downloading 'SRR5766318'... 2020-06-15T23:19:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:23:18 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:23:18 prefetch.2.10.7: 1) 'SRR5766318' was downloaded successfully 2020-06-15T23:23:18 prefetch.2.10.7: 'SRR5766318' has 0 unresolved dependencies Read 47036427 spots for SRR5766318/SRR5766318.sra Written 47036427 spots for SRR5766318/SRR5766318.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:16:28 47036427 reads; of these: 47036427 (100.00%) were unpaired; of these: 4271819 (9.08%) aligned 0 times 35550208 (75.58%) aligned exactly 1 time 7214400 (15.34%) aligned >1 times 90.92% overall alignment rate Time searching: 00:16:29 Overall time: 00:16:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 9432695 / 42764608 = 0.2206 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:56:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:56:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:56:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:56:49: 1000000 INFO @ Tue, 16 Jun 2020 08:56:55: 2000000 INFO @ Tue, 16 Jun 2020 08:57:01: 3000000 INFO @ Tue, 16 Jun 2020 08:57:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:57:12: 5000000 INFO @ Tue, 16 Jun 2020 08:57:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:57:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:57:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:57:18: 6000000 INFO @ Tue, 16 Jun 2020 08:57:19: 1000000 INFO @ Tue, 16 Jun 2020 08:57:24: 7000000 INFO @ Tue, 16 Jun 2020 08:57:25: 2000000 INFO @ Tue, 16 Jun 2020 08:57:30: 8000000 INFO @ Tue, 16 Jun 2020 08:57:31: 3000000 INFO @ Tue, 16 Jun 2020 08:57:36: 9000000 INFO @ Tue, 16 Jun 2020 08:57:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:57:42: 10000000 INFO @ Tue, 16 Jun 2020 08:57:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:57:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:57:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:57:44: 5000000 INFO @ Tue, 16 Jun 2020 08:57:48: 11000000 INFO @ Tue, 16 Jun 2020 08:57:50: 1000000 INFO @ Tue, 16 Jun 2020 08:57:50: 6000000 INFO @ Tue, 16 Jun 2020 08:57:53: 12000000 INFO @ Tue, 16 Jun 2020 08:57:56: 2000000 INFO @ Tue, 16 Jun 2020 08:57:57: 7000000 INFO @ Tue, 16 Jun 2020 08:57:59: 13000000 INFO @ Tue, 16 Jun 2020 08:58:03: 3000000 INFO @ Tue, 16 Jun 2020 08:58:04: 8000000 INFO @ Tue, 16 Jun 2020 08:58:05: 14000000 INFO @ Tue, 16 Jun 2020 08:58:10: 4000000 INFO @ Tue, 16 Jun 2020 08:58:11: 15000000 INFO @ Tue, 16 Jun 2020 08:58:11: 9000000 INFO @ Tue, 16 Jun 2020 08:58:16: 5000000 INFO @ Tue, 16 Jun 2020 08:58:17: 16000000 INFO @ Tue, 16 Jun 2020 08:58:18: 10000000 INFO @ Tue, 16 Jun 2020 08:58:23: 17000000 INFO @ Tue, 16 Jun 2020 08:58:23: 6000000 INFO @ Tue, 16 Jun 2020 08:58:26: 11000000 INFO @ Tue, 16 Jun 2020 08:58:29: 18000000 INFO @ Tue, 16 Jun 2020 08:58:30: 7000000 INFO @ Tue, 16 Jun 2020 08:58:33: 12000000 INFO @ Tue, 16 Jun 2020 08:58:35: 19000000 INFO @ Tue, 16 Jun 2020 08:58:37: 8000000 INFO @ Tue, 16 Jun 2020 08:58:40: 13000000 INFO @ Tue, 16 Jun 2020 08:58:41: 20000000 INFO @ Tue, 16 Jun 2020 08:58:44: 9000000 INFO @ Tue, 16 Jun 2020 08:58:47: 21000000 INFO @ Tue, 16 Jun 2020 08:58:47: 14000000 INFO @ Tue, 16 Jun 2020 08:58:51: 10000000 INFO @ Tue, 16 Jun 2020 08:58:54: 22000000 INFO @ Tue, 16 Jun 2020 08:58:54: 15000000 INFO @ Tue, 16 Jun 2020 08:58:59: 11000000 INFO @ Tue, 16 Jun 2020 08:59:00: 23000000 INFO @ Tue, 16 Jun 2020 08:59:02: 16000000 INFO @ Tue, 16 Jun 2020 08:59:07: 12000000 INFO @ Tue, 16 Jun 2020 08:59:07: 24000000 INFO @ Tue, 16 Jun 2020 08:59:10: 17000000 INFO @ Tue, 16 Jun 2020 08:59:13: 25000000 INFO @ Tue, 16 Jun 2020 08:59:14: 13000000 INFO @ Tue, 16 Jun 2020 08:59:17: 18000000 INFO @ Tue, 16 Jun 2020 08:59:19: 26000000 INFO @ Tue, 16 Jun 2020 08:59:21: 14000000 INFO @ Tue, 16 Jun 2020 08:59:24: 19000000 INFO @ Tue, 16 Jun 2020 08:59:25: 27000000 INFO @ Tue, 16 Jun 2020 08:59:27: 15000000 INFO @ Tue, 16 Jun 2020 08:59:31: 20000000 INFO @ Tue, 16 Jun 2020 08:59:31: 28000000 INFO @ Tue, 16 Jun 2020 08:59:34: 16000000 INFO @ Tue, 16 Jun 2020 08:59:37: 29000000 INFO @ Tue, 16 Jun 2020 08:59:38: 21000000 INFO @ Tue, 16 Jun 2020 08:59:41: 17000000 INFO @ Tue, 16 Jun 2020 08:59:43: 30000000 INFO @ Tue, 16 Jun 2020 08:59:44: 22000000 INFO @ Tue, 16 Jun 2020 08:59:48: 18000000 INFO @ Tue, 16 Jun 2020 08:59:49: 31000000 INFO @ Tue, 16 Jun 2020 08:59:51: 23000000 INFO @ Tue, 16 Jun 2020 08:59:55: 19000000 INFO @ Tue, 16 Jun 2020 08:59:55: 32000000 INFO @ Tue, 16 Jun 2020 08:59:57: 24000000 INFO @ Tue, 16 Jun 2020 09:00:01: 33000000 INFO @ Tue, 16 Jun 2020 09:00:01: 20000000 INFO @ Tue, 16 Jun 2020 09:00:03: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:00:03: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:00:03: #1 total tags in treatment: 33331913 INFO @ Tue, 16 Jun 2020 09:00:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:00:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:00:03: #1 tags after filtering in treatment: 33331913 INFO @ Tue, 16 Jun 2020 09:00:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:00:03: #1 finished! INFO @ Tue, 16 Jun 2020 09:00:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:00:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:00:04: 25000000 INFO @ Tue, 16 Jun 2020 09:00:06: #2 number of paired peaks: 58 WARNING @ Tue, 16 Jun 2020 09:00:06: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:00:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:00:08: 21000000 INFO @ Tue, 16 Jun 2020 09:00:10: 26000000 INFO @ Tue, 16 Jun 2020 09:00:14: 22000000 INFO @ Tue, 16 Jun 2020 09:00:17: 27000000 INFO @ Tue, 16 Jun 2020 09:00:21: 23000000 INFO @ Tue, 16 Jun 2020 09:00:23: 28000000 INFO @ Tue, 16 Jun 2020 09:00:27: 24000000 INFO @ Tue, 16 Jun 2020 09:00:30: 29000000 INFO @ Tue, 16 Jun 2020 09:00:34: 25000000 INFO @ Tue, 16 Jun 2020 09:00:36: 30000000 INFO @ Tue, 16 Jun 2020 09:00:40: 26000000 INFO @ Tue, 16 Jun 2020 09:00:43: 31000000 INFO @ Tue, 16 Jun 2020 09:00:47: 27000000 INFO @ Tue, 16 Jun 2020 09:00:49: 32000000 INFO @ Tue, 16 Jun 2020 09:00:53: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:00:56: 33000000 INFO @ Tue, 16 Jun 2020 09:00:58: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:00:58: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:00:58: #1 total tags in treatment: 33331913 INFO @ Tue, 16 Jun 2020 09:00:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:00:59: #1 tags after filtering in treatment: 33331913 INFO @ Tue, 16 Jun 2020 09:00:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:00:59: #1 finished! INFO @ Tue, 16 Jun 2020 09:00:59: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:00:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:01:00: 29000000 INFO @ Tue, 16 Jun 2020 09:01:01: #2 number of paired peaks: 58 WARNING @ Tue, 16 Jun 2020 09:01:01: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:01:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:01:05: 30000000 INFO @ Tue, 16 Jun 2020 09:01:11: 31000000 INFO @ Tue, 16 Jun 2020 09:01:16: 32000000 INFO @ Tue, 16 Jun 2020 09:01:22: 33000000 INFO @ Tue, 16 Jun 2020 09:01:24: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 09:01:24: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 09:01:24: #1 total tags in treatment: 33331913 INFO @ Tue, 16 Jun 2020 09:01:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:01:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:01:25: #1 tags after filtering in treatment: 33331913 INFO @ Tue, 16 Jun 2020 09:01:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:01:25: #1 finished! INFO @ Tue, 16 Jun 2020 09:01:25: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:01:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:01:27: #2 number of paired peaks: 58 WARNING @ Tue, 16 Jun 2020 09:01:27: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:01:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX2965733/SRX2965733.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。