Job ID = 6367057 SRX = SRX276915 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:13:01 prefetch.2.10.7: 1) Downloading 'SRR849521'... 2020-06-15T23:13:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:13:28 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:13:28 prefetch.2.10.7: 'SRR849521' is valid 2020-06-15T23:13:28 prefetch.2.10.7: 1) 'SRR849521' was downloaded successfully Read 6117489 spots for SRR849521/SRR849521.sra Written 6117489 spots for SRR849521/SRR849521.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:45 6117489 reads; of these: 6117489 (100.00%) were unpaired; of these: 2583348 (42.23%) aligned 0 times 2960394 (48.39%) aligned exactly 1 time 573747 (9.38%) aligned >1 times 57.77% overall alignment rate Time searching: 00:00:45 Overall time: 00:00:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 300423 / 3534141 = 0.0850 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:15:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:15:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:15:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:15:44: 1000000 INFO @ Tue, 16 Jun 2020 08:15:49: 2000000 INFO @ Tue, 16 Jun 2020 08:15:54: 3000000 INFO @ Tue, 16 Jun 2020 08:15:55: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:15:55: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:15:55: #1 total tags in treatment: 3233718 INFO @ Tue, 16 Jun 2020 08:15:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:15:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:15:55: #1 tags after filtering in treatment: 3233718 INFO @ Tue, 16 Jun 2020 08:15:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:15:55: #1 finished! INFO @ Tue, 16 Jun 2020 08:15:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:15:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:15:55: #2 number of paired peaks: 377 WARNING @ Tue, 16 Jun 2020 08:15:55: Fewer paired peaks (377) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 377 pairs to build model! INFO @ Tue, 16 Jun 2020 08:15:55: start model_add_line... INFO @ Tue, 16 Jun 2020 08:15:55: start X-correlation... INFO @ Tue, 16 Jun 2020 08:15:55: end of X-cor INFO @ Tue, 16 Jun 2020 08:15:55: #2 finished! INFO @ Tue, 16 Jun 2020 08:15:55: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:15:55: #2 alternative fragment length(s) may be 34 bps INFO @ Tue, 16 Jun 2020 08:15:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.05_model.r WARNING @ Tue, 16 Jun 2020 08:15:55: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:15:55: #2 You may need to consider one of the other alternative d(s): 34 WARNING @ Tue, 16 Jun 2020 08:15:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:15:55: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:15:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:16:02: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:16:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:16:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:16:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.05_summits.bed INFO @ Tue, 16 Jun 2020 08:16:05: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (360 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:16:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:16:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:16:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:16:14: 1000000 INFO @ Tue, 16 Jun 2020 08:16:19: 2000000 INFO @ Tue, 16 Jun 2020 08:16:24: 3000000 INFO @ Tue, 16 Jun 2020 08:16:25: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:16:25: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:16:25: #1 total tags in treatment: 3233718 INFO @ Tue, 16 Jun 2020 08:16:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:16:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:16:25: #1 tags after filtering in treatment: 3233718 INFO @ Tue, 16 Jun 2020 08:16:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:16:25: #1 finished! INFO @ Tue, 16 Jun 2020 08:16:25: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:16:25: #2 number of paired peaks: 377 WARNING @ Tue, 16 Jun 2020 08:16:25: Fewer paired peaks (377) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 377 pairs to build model! INFO @ Tue, 16 Jun 2020 08:16:25: start model_add_line... INFO @ Tue, 16 Jun 2020 08:16:25: start X-correlation... INFO @ Tue, 16 Jun 2020 08:16:25: end of X-cor INFO @ Tue, 16 Jun 2020 08:16:25: #2 finished! INFO @ Tue, 16 Jun 2020 08:16:25: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:16:25: #2 alternative fragment length(s) may be 34 bps INFO @ Tue, 16 Jun 2020 08:16:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.10_model.r WARNING @ Tue, 16 Jun 2020 08:16:25: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:16:25: #2 You may need to consider one of the other alternative d(s): 34 WARNING @ Tue, 16 Jun 2020 08:16:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:16:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:16:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:16:32: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:16:35: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:16:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:16:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.10_summits.bed INFO @ Tue, 16 Jun 2020 08:16:35: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (161 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:16:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:16:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:16:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:16:44: 1000000 INFO @ Tue, 16 Jun 2020 08:16:49: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:16:54: 3000000 INFO @ Tue, 16 Jun 2020 08:16:55: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:16:55: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:16:55: #1 total tags in treatment: 3233718 INFO @ Tue, 16 Jun 2020 08:16:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:16:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:16:55: #1 tags after filtering in treatment: 3233718 INFO @ Tue, 16 Jun 2020 08:16:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:16:55: #1 finished! INFO @ Tue, 16 Jun 2020 08:16:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:16:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:16:56: #2 number of paired peaks: 377 WARNING @ Tue, 16 Jun 2020 08:16:56: Fewer paired peaks (377) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 377 pairs to build model! INFO @ Tue, 16 Jun 2020 08:16:56: start model_add_line... INFO @ Tue, 16 Jun 2020 08:16:56: start X-correlation... INFO @ Tue, 16 Jun 2020 08:16:56: end of X-cor INFO @ Tue, 16 Jun 2020 08:16:56: #2 finished! INFO @ Tue, 16 Jun 2020 08:16:56: #2 predicted fragment length is 34 bps INFO @ Tue, 16 Jun 2020 08:16:56: #2 alternative fragment length(s) may be 34 bps INFO @ Tue, 16 Jun 2020 08:16:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.20_model.r WARNING @ Tue, 16 Jun 2020 08:16:56: #2 Since the d (34) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:16:56: #2 You may need to consider one of the other alternative d(s): 34 WARNING @ Tue, 16 Jun 2020 08:16:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:16:56: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:16:56: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:17:02: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:17:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:17:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:17:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX276915/SRX276915.20_summits.bed INFO @ Tue, 16 Jun 2020 08:17:06: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (46 records, 4 fields): 1 millis CompletedMACS2peakCalling