Job ID = 6366794 SRX = SRX233459 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:01:53 prefetch.2.10.7: 1) Downloading 'SRR701515'... 2020-06-15T23:01:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:02:21 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:02:21 prefetch.2.10.7: 'SRR701515' is valid 2020-06-15T23:02:21 prefetch.2.10.7: 1) 'SRR701515' was downloaded successfully Read 4021791 spots for SRR701515/SRR701515.sra Written 4021791 spots for SRR701515/SRR701515.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:40 4021791 reads; of these: 4021791 (100.00%) were unpaired; of these: 202510 (5.04%) aligned 0 times 3228236 (80.27%) aligned exactly 1 time 591045 (14.70%) aligned >1 times 94.96% overall alignment rate Time searching: 00:00:40 Overall time: 00:00:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 208075 / 3819281 = 0.0545 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:04:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:04:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:04:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:04:33: 1000000 INFO @ Tue, 16 Jun 2020 08:04:39: 2000000 INFO @ Tue, 16 Jun 2020 08:04:45: 3000000 INFO @ Tue, 16 Jun 2020 08:04:49: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:04:49: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:04:49: #1 total tags in treatment: 3611206 INFO @ Tue, 16 Jun 2020 08:04:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:04:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:04:49: #1 tags after filtering in treatment: 3611206 INFO @ Tue, 16 Jun 2020 08:04:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:04:49: #1 finished! INFO @ Tue, 16 Jun 2020 08:04:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:04:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:04:49: #2 number of paired peaks: 436 WARNING @ Tue, 16 Jun 2020 08:04:49: Fewer paired peaks (436) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 436 pairs to build model! INFO @ Tue, 16 Jun 2020 08:04:49: start model_add_line... INFO @ Tue, 16 Jun 2020 08:04:49: start X-correlation... INFO @ Tue, 16 Jun 2020 08:04:49: end of X-cor INFO @ Tue, 16 Jun 2020 08:04:49: #2 finished! INFO @ Tue, 16 Jun 2020 08:04:49: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:04:49: #2 alternative fragment length(s) may be 4,33,557,567 bps INFO @ Tue, 16 Jun 2020 08:04:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.05_model.r WARNING @ Tue, 16 Jun 2020 08:04:49: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:04:49: #2 You may need to consider one of the other alternative d(s): 4,33,557,567 WARNING @ Tue, 16 Jun 2020 08:04:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:04:49: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:04:49: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:04:57: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:04:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:04:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:04:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:05:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:05:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:05:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.05_summits.bed INFO @ Tue, 16 Jun 2020 08:05:01: Done! INFO @ Tue, 16 Jun 2020 08:05:03: 1000000 pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (396 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:05:09: 2000000 INFO @ Tue, 16 Jun 2020 08:05:15: 3000000 INFO @ Tue, 16 Jun 2020 08:05:18: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:05:18: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:05:18: #1 total tags in treatment: 3611206 INFO @ Tue, 16 Jun 2020 08:05:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:05:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:05:18: #1 tags after filtering in treatment: 3611206 INFO @ Tue, 16 Jun 2020 08:05:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:05:18: #1 finished! INFO @ Tue, 16 Jun 2020 08:05:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:05:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:05:18: #2 number of paired peaks: 436 WARNING @ Tue, 16 Jun 2020 08:05:18: Fewer paired peaks (436) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 436 pairs to build model! INFO @ Tue, 16 Jun 2020 08:05:18: start model_add_line... INFO @ Tue, 16 Jun 2020 08:05:18: start X-correlation... INFO @ Tue, 16 Jun 2020 08:05:18: end of X-cor INFO @ Tue, 16 Jun 2020 08:05:18: #2 finished! INFO @ Tue, 16 Jun 2020 08:05:18: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:05:18: #2 alternative fragment length(s) may be 4,33,557,567 bps INFO @ Tue, 16 Jun 2020 08:05:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.10_model.r WARNING @ Tue, 16 Jun 2020 08:05:18: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:05:18: #2 You may need to consider one of the other alternative d(s): 4,33,557,567 WARNING @ Tue, 16 Jun 2020 08:05:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:05:18: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:05:18: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:05:27: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:05:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:05:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:05:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:05:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:05:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:05:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.10_summits.bed INFO @ Tue, 16 Jun 2020 08:05:31: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (191 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:05:33: 1000000 INFO @ Tue, 16 Jun 2020 08:05:39: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:05:45: 3000000 INFO @ Tue, 16 Jun 2020 08:05:49: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:05:49: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:05:49: #1 total tags in treatment: 3611206 INFO @ Tue, 16 Jun 2020 08:05:49: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:05:49: #1 tags after filtering in treatment: 3611206 INFO @ Tue, 16 Jun 2020 08:05:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:05:49: #1 finished! INFO @ Tue, 16 Jun 2020 08:05:49: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:05:49: #2 number of paired peaks: 436 WARNING @ Tue, 16 Jun 2020 08:05:49: Fewer paired peaks (436) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 436 pairs to build model! INFO @ Tue, 16 Jun 2020 08:05:49: start model_add_line... INFO @ Tue, 16 Jun 2020 08:05:49: start X-correlation... INFO @ Tue, 16 Jun 2020 08:05:49: end of X-cor INFO @ Tue, 16 Jun 2020 08:05:49: #2 finished! INFO @ Tue, 16 Jun 2020 08:05:49: #2 predicted fragment length is 33 bps INFO @ Tue, 16 Jun 2020 08:05:49: #2 alternative fragment length(s) may be 4,33,557,567 bps INFO @ Tue, 16 Jun 2020 08:05:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.20_model.r WARNING @ Tue, 16 Jun 2020 08:05:49: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:05:49: #2 You may need to consider one of the other alternative d(s): 4,33,557,567 WARNING @ Tue, 16 Jun 2020 08:05:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:05:49: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:05:49: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:05:57: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:06:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:06:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:06:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233459/SRX233459.20_summits.bed INFO @ Tue, 16 Jun 2020 08:06:01: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (38 records, 4 fields): 1 millis CompletedMACS2peakCalling