Job ID = 6366791 SRX = SRX233456 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:22:16 prefetch.2.10.7: 1) Downloading 'SRR701512'... 2020-06-15T23:22:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:22:45 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:22:45 prefetch.2.10.7: 'SRR701512' is valid 2020-06-15T23:22:45 prefetch.2.10.7: 1) 'SRR701512' was downloaded successfully Read 5538997 spots for SRR701512/SRR701512.sra Written 5538997 spots for SRR701512/SRR701512.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:48 5538997 reads; of these: 5538997 (100.00%) were unpaired; of these: 970102 (17.51%) aligned 0 times 3817671 (68.92%) aligned exactly 1 time 751224 (13.56%) aligned >1 times 82.49% overall alignment rate Time searching: 00:00:48 Overall time: 00:00:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 230582 / 4568895 = 0.0505 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:25:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:25:15: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:25:15: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:25:21: 1000000 INFO @ Tue, 16 Jun 2020 08:25:26: 2000000 INFO @ Tue, 16 Jun 2020 08:25:32: 3000000 INFO @ Tue, 16 Jun 2020 08:25:38: 4000000 INFO @ Tue, 16 Jun 2020 08:25:39: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:25:39: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:25:39: #1 total tags in treatment: 4338313 INFO @ Tue, 16 Jun 2020 08:25:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:25:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:25:40: #1 tags after filtering in treatment: 4338313 INFO @ Tue, 16 Jun 2020 08:25:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:25:40: #1 finished! INFO @ Tue, 16 Jun 2020 08:25:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:25:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:25:40: #2 number of paired peaks: 415 WARNING @ Tue, 16 Jun 2020 08:25:40: Fewer paired peaks (415) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 415 pairs to build model! INFO @ Tue, 16 Jun 2020 08:25:40: start model_add_line... INFO @ Tue, 16 Jun 2020 08:25:40: start X-correlation... INFO @ Tue, 16 Jun 2020 08:25:40: end of X-cor INFO @ Tue, 16 Jun 2020 08:25:40: #2 finished! INFO @ Tue, 16 Jun 2020 08:25:40: #2 predicted fragment length is 32 bps INFO @ Tue, 16 Jun 2020 08:25:40: #2 alternative fragment length(s) may be 3,32,532,537,551 bps INFO @ Tue, 16 Jun 2020 08:25:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.05_model.r WARNING @ Tue, 16 Jun 2020 08:25:40: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:25:40: #2 You may need to consider one of the other alternative d(s): 3,32,532,537,551 WARNING @ Tue, 16 Jun 2020 08:25:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:25:40: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:25:40: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:25:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:25:45: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:25:45: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:25:50: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:25:51: 1000000 INFO @ Tue, 16 Jun 2020 08:25:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:25:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:25:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.05_summits.bed INFO @ Tue, 16 Jun 2020 08:25:55: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (453 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:25:56: 2000000 INFO @ Tue, 16 Jun 2020 08:26:02: 3000000 INFO @ Tue, 16 Jun 2020 08:26:07: 4000000 INFO @ Tue, 16 Jun 2020 08:26:09: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:26:09: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:26:09: #1 total tags in treatment: 4338313 INFO @ Tue, 16 Jun 2020 08:26:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:26:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:26:09: #1 tags after filtering in treatment: 4338313 INFO @ Tue, 16 Jun 2020 08:26:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:26:09: #1 finished! INFO @ Tue, 16 Jun 2020 08:26:09: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:26:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:26:09: #2 number of paired peaks: 415 WARNING @ Tue, 16 Jun 2020 08:26:09: Fewer paired peaks (415) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 415 pairs to build model! INFO @ Tue, 16 Jun 2020 08:26:09: start model_add_line... INFO @ Tue, 16 Jun 2020 08:26:09: start X-correlation... INFO @ Tue, 16 Jun 2020 08:26:09: end of X-cor INFO @ Tue, 16 Jun 2020 08:26:09: #2 finished! INFO @ Tue, 16 Jun 2020 08:26:09: #2 predicted fragment length is 32 bps INFO @ Tue, 16 Jun 2020 08:26:09: #2 alternative fragment length(s) may be 3,32,532,537,551 bps INFO @ Tue, 16 Jun 2020 08:26:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.10_model.r WARNING @ Tue, 16 Jun 2020 08:26:09: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:26:09: #2 You may need to consider one of the other alternative d(s): 3,32,532,537,551 WARNING @ Tue, 16 Jun 2020 08:26:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:26:09: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:26:09: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:26:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:26:15: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:26:15: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:26:19: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:26:21: 1000000 INFO @ Tue, 16 Jun 2020 08:26:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:26:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:26:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.10_summits.bed INFO @ Tue, 16 Jun 2020 08:26:24: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (188 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:26:27: 2000000 INFO @ Tue, 16 Jun 2020 08:26:32: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:26:38: 4000000 INFO @ Tue, 16 Jun 2020 08:26:40: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:26:40: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:26:40: #1 total tags in treatment: 4338313 INFO @ Tue, 16 Jun 2020 08:26:40: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:26:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:26:40: #1 tags after filtering in treatment: 4338313 INFO @ Tue, 16 Jun 2020 08:26:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:26:40: #1 finished! INFO @ Tue, 16 Jun 2020 08:26:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:26:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:26:40: #2 number of paired peaks: 415 WARNING @ Tue, 16 Jun 2020 08:26:40: Fewer paired peaks (415) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 415 pairs to build model! INFO @ Tue, 16 Jun 2020 08:26:40: start model_add_line... INFO @ Tue, 16 Jun 2020 08:26:40: start X-correlation... INFO @ Tue, 16 Jun 2020 08:26:40: end of X-cor INFO @ Tue, 16 Jun 2020 08:26:40: #2 finished! INFO @ Tue, 16 Jun 2020 08:26:40: #2 predicted fragment length is 32 bps INFO @ Tue, 16 Jun 2020 08:26:40: #2 alternative fragment length(s) may be 3,32,532,537,551 bps INFO @ Tue, 16 Jun 2020 08:26:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.20_model.r WARNING @ Tue, 16 Jun 2020 08:26:40: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:26:40: #2 You may need to consider one of the other alternative d(s): 3,32,532,537,551 WARNING @ Tue, 16 Jun 2020 08:26:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:26:40: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:26:40: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:26:50: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:26:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:26:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:26:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX233456/SRX233456.20_summits.bed INFO @ Tue, 16 Jun 2020 08:26:55: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (39 records, 4 fields): 1 millis CompletedMACS2peakCalling