Job ID = 6366519 SRX = SRX1674099 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:47:37 prefetch.2.10.7: 1) Downloading 'SRR3320145'... 2020-06-15T22:47:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:57:30 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:57:30 prefetch.2.10.7: 1) 'SRR3320145' was downloaded successfully Read 104108339 spots for SRR3320145/SRR3320145.sra Written 104108339 spots for SRR3320145/SRR3320145.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:19 104108339 reads; of these: 104108339 (100.00%) were unpaired; of these: 4860758 (4.67%) aligned 0 times 88148166 (84.67%) aligned exactly 1 time 11099415 (10.66%) aligned >1 times 95.33% overall alignment rate Time searching: 00:44:20 Overall time: 00:44:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 51840465 / 99247581 = 0.5223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:19:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:19:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:19:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:19:35: 1000000 INFO @ Tue, 16 Jun 2020 09:19:43: 2000000 INFO @ Tue, 16 Jun 2020 09:19:51: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:19:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:19:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:19:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:19:59: 4000000 INFO @ Tue, 16 Jun 2020 09:20:06: 1000000 INFO @ Tue, 16 Jun 2020 09:20:08: 5000000 INFO @ Tue, 16 Jun 2020 09:20:14: 2000000 INFO @ Tue, 16 Jun 2020 09:20:17: 6000000 INFO @ Tue, 16 Jun 2020 09:20:23: 3000000 INFO @ Tue, 16 Jun 2020 09:20:25: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:20:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:20:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:20:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:20:31: 4000000 INFO @ Tue, 16 Jun 2020 09:20:33: 8000000 INFO @ Tue, 16 Jun 2020 09:20:36: 1000000 INFO @ Tue, 16 Jun 2020 09:20:40: 5000000 INFO @ Tue, 16 Jun 2020 09:20:42: 9000000 INFO @ Tue, 16 Jun 2020 09:20:45: 2000000 INFO @ Tue, 16 Jun 2020 09:20:49: 6000000 INFO @ Tue, 16 Jun 2020 09:20:50: 10000000 INFO @ Tue, 16 Jun 2020 09:20:54: 3000000 INFO @ Tue, 16 Jun 2020 09:20:57: 7000000 INFO @ Tue, 16 Jun 2020 09:20:58: 11000000 INFO @ Tue, 16 Jun 2020 09:21:02: 4000000 INFO @ Tue, 16 Jun 2020 09:21:06: 8000000 INFO @ Tue, 16 Jun 2020 09:21:06: 12000000 INFO @ Tue, 16 Jun 2020 09:21:10: 5000000 INFO @ Tue, 16 Jun 2020 09:21:14: 9000000 INFO @ Tue, 16 Jun 2020 09:21:14: 13000000 INFO @ Tue, 16 Jun 2020 09:21:19: 6000000 INFO @ Tue, 16 Jun 2020 09:21:22: 10000000 INFO @ Tue, 16 Jun 2020 09:21:23: 14000000 INFO @ Tue, 16 Jun 2020 09:21:27: 7000000 INFO @ Tue, 16 Jun 2020 09:21:30: 11000000 INFO @ Tue, 16 Jun 2020 09:21:31: 15000000 INFO @ Tue, 16 Jun 2020 09:21:35: 8000000 INFO @ Tue, 16 Jun 2020 09:21:38: 12000000 INFO @ Tue, 16 Jun 2020 09:21:39: 16000000 INFO @ Tue, 16 Jun 2020 09:21:43: 9000000 INFO @ Tue, 16 Jun 2020 09:21:46: 13000000 INFO @ Tue, 16 Jun 2020 09:21:47: 17000000 INFO @ Tue, 16 Jun 2020 09:21:51: 10000000 INFO @ Tue, 16 Jun 2020 09:21:55: 14000000 INFO @ Tue, 16 Jun 2020 09:21:55: 18000000 INFO @ Tue, 16 Jun 2020 09:21:59: 11000000 INFO @ Tue, 16 Jun 2020 09:22:03: 15000000 INFO @ Tue, 16 Jun 2020 09:22:03: 19000000 INFO @ Tue, 16 Jun 2020 09:22:07: 12000000 INFO @ Tue, 16 Jun 2020 09:22:11: 20000000 INFO @ Tue, 16 Jun 2020 09:22:11: 16000000 INFO @ Tue, 16 Jun 2020 09:22:16: 13000000 INFO @ Tue, 16 Jun 2020 09:22:19: 17000000 INFO @ Tue, 16 Jun 2020 09:22:19: 21000000 INFO @ Tue, 16 Jun 2020 09:22:24: 14000000 INFO @ Tue, 16 Jun 2020 09:22:28: 18000000 INFO @ Tue, 16 Jun 2020 09:22:28: 22000000 INFO @ Tue, 16 Jun 2020 09:22:32: 15000000 INFO @ Tue, 16 Jun 2020 09:22:36: 19000000 INFO @ Tue, 16 Jun 2020 09:22:37: 23000000 INFO @ Tue, 16 Jun 2020 09:22:40: 16000000 INFO @ Tue, 16 Jun 2020 09:22:44: 20000000 INFO @ Tue, 16 Jun 2020 09:22:46: 24000000 INFO @ Tue, 16 Jun 2020 09:22:48: 17000000 INFO @ Tue, 16 Jun 2020 09:22:52: 21000000 INFO @ Tue, 16 Jun 2020 09:22:55: 25000000 INFO @ Tue, 16 Jun 2020 09:22:56: 18000000 INFO @ Tue, 16 Jun 2020 09:23:01: 22000000 INFO @ Tue, 16 Jun 2020 09:23:03: 26000000 INFO @ Tue, 16 Jun 2020 09:23:04: 19000000 INFO @ Tue, 16 Jun 2020 09:23:09: 23000000 INFO @ Tue, 16 Jun 2020 09:23:11: 27000000 INFO @ Tue, 16 Jun 2020 09:23:12: 20000000 INFO @ Tue, 16 Jun 2020 09:23:16: 24000000 INFO @ Tue, 16 Jun 2020 09:23:18: 28000000 INFO @ Tue, 16 Jun 2020 09:23:20: 21000000 INFO @ Tue, 16 Jun 2020 09:23:24: 25000000 INFO @ Tue, 16 Jun 2020 09:23:26: 29000000 INFO @ Tue, 16 Jun 2020 09:23:28: 22000000 INFO @ Tue, 16 Jun 2020 09:23:32: 26000000 INFO @ Tue, 16 Jun 2020 09:23:34: 30000000 INFO @ Tue, 16 Jun 2020 09:23:36: 23000000 INFO @ Tue, 16 Jun 2020 09:23:40: 27000000 INFO @ Tue, 16 Jun 2020 09:23:42: 31000000 INFO @ Tue, 16 Jun 2020 09:23:44: 24000000 INFO @ Tue, 16 Jun 2020 09:23:48: 28000000 INFO @ Tue, 16 Jun 2020 09:23:49: 32000000 INFO @ Tue, 16 Jun 2020 09:23:52: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:23:56: 29000000 INFO @ Tue, 16 Jun 2020 09:23:57: 33000000 INFO @ Tue, 16 Jun 2020 09:24:00: 26000000 INFO @ Tue, 16 Jun 2020 09:24:04: 30000000 INFO @ Tue, 16 Jun 2020 09:24:05: 34000000 INFO @ Tue, 16 Jun 2020 09:24:08: 27000000 INFO @ Tue, 16 Jun 2020 09:24:13: 31000000 INFO @ Tue, 16 Jun 2020 09:24:13: 35000000 INFO @ Tue, 16 Jun 2020 09:24:17: 28000000 INFO @ Tue, 16 Jun 2020 09:24:21: 32000000 INFO @ Tue, 16 Jun 2020 09:24:21: 36000000 INFO @ Tue, 16 Jun 2020 09:24:25: 29000000 INFO @ Tue, 16 Jun 2020 09:24:29: 33000000 INFO @ Tue, 16 Jun 2020 09:24:29: 37000000 INFO @ Tue, 16 Jun 2020 09:24:33: 30000000 INFO @ Tue, 16 Jun 2020 09:24:37: 38000000 INFO @ Tue, 16 Jun 2020 09:24:37: 34000000 INFO @ Tue, 16 Jun 2020 09:24:41: 31000000 INFO @ Tue, 16 Jun 2020 09:24:45: 35000000 INFO @ Tue, 16 Jun 2020 09:24:45: 39000000 INFO @ Tue, 16 Jun 2020 09:24:49: 32000000 INFO @ Tue, 16 Jun 2020 09:24:53: 36000000 INFO @ Tue, 16 Jun 2020 09:24:53: 40000000 INFO @ Tue, 16 Jun 2020 09:24:57: 33000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:25:01: 37000000 INFO @ Tue, 16 Jun 2020 09:25:01: 41000000 INFO @ Tue, 16 Jun 2020 09:25:05: 34000000 INFO @ Tue, 16 Jun 2020 09:25:09: 38000000 INFO @ Tue, 16 Jun 2020 09:25:09: 42000000 INFO @ Tue, 16 Jun 2020 09:25:13: 35000000 INFO @ Tue, 16 Jun 2020 09:25:17: 43000000 INFO @ Tue, 16 Jun 2020 09:25:17: 39000000 INFO @ Tue, 16 Jun 2020 09:25:21: 36000000 INFO @ Tue, 16 Jun 2020 09:25:25: 44000000 INFO @ Tue, 16 Jun 2020 09:25:25: 40000000 INFO @ Tue, 16 Jun 2020 09:25:29: 37000000 INFO @ Tue, 16 Jun 2020 09:25:33: 41000000 INFO @ Tue, 16 Jun 2020 09:25:33: 45000000 INFO @ Tue, 16 Jun 2020 09:25:38: 38000000 INFO @ Tue, 16 Jun 2020 09:25:41: 42000000 INFO @ Tue, 16 Jun 2020 09:25:42: 46000000 INFO @ Tue, 16 Jun 2020 09:25:46: 39000000 INFO @ Tue, 16 Jun 2020 09:25:49: 43000000 INFO @ Tue, 16 Jun 2020 09:25:50: 47000000 INFO @ Tue, 16 Jun 2020 09:25:54: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:25:54: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:25:54: #1 total tags in treatment: 47407116 INFO @ Tue, 16 Jun 2020 09:25:54: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:25:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:25:55: 40000000 INFO @ Tue, 16 Jun 2020 09:25:55: #1 tags after filtering in treatment: 47407116 INFO @ Tue, 16 Jun 2020 09:25:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:25:55: #1 finished! INFO @ Tue, 16 Jun 2020 09:25:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:25:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:25:56: 44000000 INFO @ Tue, 16 Jun 2020 09:25:58: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:25:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:25:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:26:03: 41000000 INFO @ Tue, 16 Jun 2020 09:26:04: 45000000 INFO @ Tue, 16 Jun 2020 09:26:11: 42000000 INFO @ Tue, 16 Jun 2020 09:26:12: 46000000 INFO @ Tue, 16 Jun 2020 09:26:18: 43000000 INFO @ Tue, 16 Jun 2020 09:26:20: 47000000 INFO @ Tue, 16 Jun 2020 09:26:23: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:26:23: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:26:23: #1 total tags in treatment: 47407116 INFO @ Tue, 16 Jun 2020 09:26:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:26:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:26:24: #1 tags after filtering in treatment: 47407116 INFO @ Tue, 16 Jun 2020 09:26:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:26:24: #1 finished! INFO @ Tue, 16 Jun 2020 09:26:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:26:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:26:26: 44000000 INFO @ Tue, 16 Jun 2020 09:26:27: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:26:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:26:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:26:33: 45000000 INFO @ Tue, 16 Jun 2020 09:26:40: 46000000 INFO @ Tue, 16 Jun 2020 09:26:47: 47000000 INFO @ Tue, 16 Jun 2020 09:26:50: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 09:26:50: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 09:26:50: #1 total tags in treatment: 47407116 INFO @ Tue, 16 Jun 2020 09:26:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:26:51: #1 tags after filtering in treatment: 47407116 INFO @ Tue, 16 Jun 2020 09:26:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:26:51: #1 finished! INFO @ Tue, 16 Jun 2020 09:26:51: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:26:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:26:54: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 09:26:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:26:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674099/SRX1674099.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling