Job ID = 6366518 SRX = SRX1674098 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:34:35 prefetch.2.10.7: 1) Downloading 'SRR3320144'... 2020-06-15T23:34:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:55:40 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:55:40 prefetch.2.10.7: 1) 'SRR3320144' was downloaded successfully Read 100922140 spots for SRR3320144/SRR3320144.sra Written 100922140 spots for SRR3320144/SRR3320144.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:49:12 100922140 reads; of these: 100922140 (100.00%) were unpaired; of these: 4574594 (4.53%) aligned 0 times 85508676 (84.73%) aligned exactly 1 time 10838870 (10.74%) aligned >1 times 95.47% overall alignment rate Time searching: 00:49:12 Overall time: 00:49:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 50198839 / 96347546 = 0.5210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:27:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:27:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:27:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:27:56: 1000000 INFO @ Tue, 16 Jun 2020 10:28:05: 2000000 INFO @ Tue, 16 Jun 2020 10:28:14: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:28:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:28:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:28:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:28:23: 4000000 INFO @ Tue, 16 Jun 2020 10:28:27: 1000000 INFO @ Tue, 16 Jun 2020 10:28:32: 5000000 INFO @ Tue, 16 Jun 2020 10:28:37: 2000000 INFO @ Tue, 16 Jun 2020 10:28:40: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:28:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:28:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:28:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:28:47: 3000000 INFO @ Tue, 16 Jun 2020 10:28:49: 7000000 INFO @ Tue, 16 Jun 2020 10:28:56: 1000000 INFO @ Tue, 16 Jun 2020 10:28:57: 4000000 INFO @ Tue, 16 Jun 2020 10:28:58: 8000000 INFO @ Tue, 16 Jun 2020 10:29:05: 2000000 INFO @ Tue, 16 Jun 2020 10:29:07: 9000000 INFO @ Tue, 16 Jun 2020 10:29:07: 5000000 INFO @ Tue, 16 Jun 2020 10:29:14: 3000000 INFO @ Tue, 16 Jun 2020 10:29:16: 10000000 INFO @ Tue, 16 Jun 2020 10:29:18: 6000000 INFO @ Tue, 16 Jun 2020 10:29:23: 4000000 INFO @ Tue, 16 Jun 2020 10:29:26: 11000000 INFO @ Tue, 16 Jun 2020 10:29:28: 7000000 INFO @ Tue, 16 Jun 2020 10:29:32: 5000000 INFO @ Tue, 16 Jun 2020 10:29:35: 12000000 INFO @ Tue, 16 Jun 2020 10:29:38: 8000000 INFO @ Tue, 16 Jun 2020 10:29:40: 6000000 INFO @ Tue, 16 Jun 2020 10:29:44: 13000000 INFO @ Tue, 16 Jun 2020 10:29:48: 9000000 INFO @ Tue, 16 Jun 2020 10:29:49: 7000000 INFO @ Tue, 16 Jun 2020 10:29:53: 14000000 INFO @ Tue, 16 Jun 2020 10:29:58: 8000000 INFO @ Tue, 16 Jun 2020 10:29:58: 10000000 INFO @ Tue, 16 Jun 2020 10:30:02: 15000000 INFO @ Tue, 16 Jun 2020 10:30:07: 9000000 INFO @ Tue, 16 Jun 2020 10:30:09: 11000000 INFO @ Tue, 16 Jun 2020 10:30:11: 16000000 INFO @ Tue, 16 Jun 2020 10:30:16: 10000000 INFO @ Tue, 16 Jun 2020 10:30:19: 12000000 INFO @ Tue, 16 Jun 2020 10:30:20: 17000000 INFO @ Tue, 16 Jun 2020 10:30:25: 11000000 INFO @ Tue, 16 Jun 2020 10:30:29: 13000000 INFO @ Tue, 16 Jun 2020 10:30:29: 18000000 INFO @ Tue, 16 Jun 2020 10:30:34: 12000000 INFO @ Tue, 16 Jun 2020 10:30:38: 19000000 INFO @ Tue, 16 Jun 2020 10:30:39: 14000000 INFO @ Tue, 16 Jun 2020 10:30:42: 13000000 INFO @ Tue, 16 Jun 2020 10:30:47: 20000000 INFO @ Tue, 16 Jun 2020 10:30:49: 15000000 INFO @ Tue, 16 Jun 2020 10:30:51: 14000000 INFO @ Tue, 16 Jun 2020 10:30:56: 21000000 INFO @ Tue, 16 Jun 2020 10:30:59: 16000000 INFO @ Tue, 16 Jun 2020 10:31:00: 15000000 INFO @ Tue, 16 Jun 2020 10:31:05: 22000000 INFO @ Tue, 16 Jun 2020 10:31:09: 16000000 INFO @ Tue, 16 Jun 2020 10:31:09: 17000000 INFO @ Tue, 16 Jun 2020 10:31:14: 23000000 INFO @ Tue, 16 Jun 2020 10:31:18: 17000000 INFO @ Tue, 16 Jun 2020 10:31:19: 18000000 INFO @ Tue, 16 Jun 2020 10:31:23: 24000000 INFO @ Tue, 16 Jun 2020 10:31:27: 18000000 INFO @ Tue, 16 Jun 2020 10:31:30: 19000000 INFO @ Tue, 16 Jun 2020 10:31:32: 25000000 INFO @ Tue, 16 Jun 2020 10:31:36: 19000000 INFO @ Tue, 16 Jun 2020 10:31:40: 20000000 INFO @ Tue, 16 Jun 2020 10:31:41: 26000000 INFO @ Tue, 16 Jun 2020 10:31:44: 20000000 INFO @ Tue, 16 Jun 2020 10:31:50: 21000000 INFO @ Tue, 16 Jun 2020 10:31:50: 27000000 INFO @ Tue, 16 Jun 2020 10:31:53: 21000000 INFO @ Tue, 16 Jun 2020 10:31:59: 28000000 INFO @ Tue, 16 Jun 2020 10:32:00: 22000000 INFO @ Tue, 16 Jun 2020 10:32:02: 22000000 INFO @ Tue, 16 Jun 2020 10:32:08: 29000000 INFO @ Tue, 16 Jun 2020 10:32:10: 23000000 INFO @ Tue, 16 Jun 2020 10:32:11: 23000000 INFO @ Tue, 16 Jun 2020 10:32:17: 30000000 INFO @ Tue, 16 Jun 2020 10:32:20: 24000000 INFO @ Tue, 16 Jun 2020 10:32:21: 24000000 INFO @ Tue, 16 Jun 2020 10:32:26: 31000000 INFO @ Tue, 16 Jun 2020 10:32:29: 25000000 INFO @ Tue, 16 Jun 2020 10:32:31: 25000000 INFO @ Tue, 16 Jun 2020 10:32:35: 32000000 INFO @ Tue, 16 Jun 2020 10:32:38: 26000000 INFO @ Tue, 16 Jun 2020 10:32:41: 26000000 INFO @ Tue, 16 Jun 2020 10:32:45: 33000000 INFO @ Tue, 16 Jun 2020 10:32:47: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:32:51: 27000000 INFO @ Tue, 16 Jun 2020 10:32:54: 34000000 INFO @ Tue, 16 Jun 2020 10:32:56: 28000000 INFO @ Tue, 16 Jun 2020 10:33:02: 28000000 INFO @ Tue, 16 Jun 2020 10:33:03: 35000000 INFO @ Tue, 16 Jun 2020 10:33:05: 29000000 INFO @ Tue, 16 Jun 2020 10:33:12: 36000000 INFO @ Tue, 16 Jun 2020 10:33:12: 29000000 INFO @ Tue, 16 Jun 2020 10:33:14: 30000000 INFO @ Tue, 16 Jun 2020 10:33:21: 37000000 INFO @ Tue, 16 Jun 2020 10:33:22: 30000000 INFO @ Tue, 16 Jun 2020 10:33:23: 31000000 INFO @ Tue, 16 Jun 2020 10:33:30: 38000000 INFO @ Tue, 16 Jun 2020 10:33:32: 32000000 INFO @ Tue, 16 Jun 2020 10:33:32: 31000000 INFO @ Tue, 16 Jun 2020 10:33:39: 39000000 INFO @ Tue, 16 Jun 2020 10:33:40: 33000000 INFO @ Tue, 16 Jun 2020 10:33:43: 32000000 INFO @ Tue, 16 Jun 2020 10:33:48: 40000000 INFO @ Tue, 16 Jun 2020 10:33:49: 34000000 INFO @ Tue, 16 Jun 2020 10:33:53: 33000000 INFO @ Tue, 16 Jun 2020 10:33:56: 41000000 INFO @ Tue, 16 Jun 2020 10:33:57: 35000000 INFO @ Tue, 16 Jun 2020 10:34:03: 34000000 INFO @ Tue, 16 Jun 2020 10:34:04: 42000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:34:05: 36000000 INFO @ Tue, 16 Jun 2020 10:34:13: 43000000 INFO @ Tue, 16 Jun 2020 10:34:13: 37000000 INFO @ Tue, 16 Jun 2020 10:34:13: 35000000 INFO @ Tue, 16 Jun 2020 10:34:21: 44000000 INFO @ Tue, 16 Jun 2020 10:34:21: 38000000 INFO @ Tue, 16 Jun 2020 10:34:24: 36000000 INFO @ Tue, 16 Jun 2020 10:34:29: 45000000 INFO @ Tue, 16 Jun 2020 10:34:29: 39000000 INFO @ Tue, 16 Jun 2020 10:34:34: 37000000 INFO @ Tue, 16 Jun 2020 10:34:37: 46000000 INFO @ Tue, 16 Jun 2020 10:34:37: 40000000 INFO @ Tue, 16 Jun 2020 10:34:39: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 10:34:39: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 10:34:39: #1 total tags in treatment: 46148707 INFO @ Tue, 16 Jun 2020 10:34:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:34:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:34:40: #1 tags after filtering in treatment: 46148707 INFO @ Tue, 16 Jun 2020 10:34:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:34:40: #1 finished! INFO @ Tue, 16 Jun 2020 10:34:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:34:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:34:43: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:34:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:34:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:34:44: 38000000 INFO @ Tue, 16 Jun 2020 10:34:45: 41000000 INFO @ Tue, 16 Jun 2020 10:34:53: 42000000 INFO @ Tue, 16 Jun 2020 10:34:54: 39000000 INFO @ Tue, 16 Jun 2020 10:35:00: 43000000 INFO @ Tue, 16 Jun 2020 10:35:05: 40000000 INFO @ Tue, 16 Jun 2020 10:35:08: 44000000 INFO @ Tue, 16 Jun 2020 10:35:15: 41000000 INFO @ Tue, 16 Jun 2020 10:35:15: 45000000 INFO @ Tue, 16 Jun 2020 10:35:23: 46000000 INFO @ Tue, 16 Jun 2020 10:35:24: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 10:35:24: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 10:35:24: #1 total tags in treatment: 46148707 INFO @ Tue, 16 Jun 2020 10:35:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:35:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:35:25: #1 tags after filtering in treatment: 46148707 INFO @ Tue, 16 Jun 2020 10:35:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:35:25: #1 finished! INFO @ Tue, 16 Jun 2020 10:35:25: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:35:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:35:25: 42000000 INFO @ Tue, 16 Jun 2020 10:35:28: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:35:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:35:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:35:35: 43000000 INFO @ Tue, 16 Jun 2020 10:35:46: 44000000 INFO @ Tue, 16 Jun 2020 10:35:56: 45000000 INFO @ Tue, 16 Jun 2020 10:36:05: 46000000 INFO @ Tue, 16 Jun 2020 10:36:07: #1 tag size is determined as 101 bps INFO @ Tue, 16 Jun 2020 10:36:07: #1 tag size = 101 INFO @ Tue, 16 Jun 2020 10:36:07: #1 total tags in treatment: 46148707 INFO @ Tue, 16 Jun 2020 10:36:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:36:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:36:08: #1 tags after filtering in treatment: 46148707 INFO @ Tue, 16 Jun 2020 10:36:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:36:08: #1 finished! INFO @ Tue, 16 Jun 2020 10:36:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:36:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:36:11: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:36:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:36:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674098/SRX1674098.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling