Job ID = 6366509 SRX = SRX1674089 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:58:08 prefetch.2.10.7: 1) Downloading 'SRR3320135'... 2020-06-15T22:58:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:01:24 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:01:24 prefetch.2.10.7: 1) 'SRR3320135' was downloaded successfully Read 74193295 spots for SRR3320135/SRR3320135.sra Written 74193295 spots for SRR3320135/SRR3320135.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:29 74193295 reads; of these: 74193295 (100.00%) were unpaired; of these: 9004430 (12.14%) aligned 0 times 53666900 (72.33%) aligned exactly 1 time 11521965 (15.53%) aligned >1 times 87.86% overall alignment rate Time searching: 00:15:29 Overall time: 00:15:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 20193303 / 65188865 = 0.3098 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:38:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:38:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:38:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:39:01: 1000000 INFO @ Tue, 16 Jun 2020 08:39:07: 2000000 INFO @ Tue, 16 Jun 2020 08:39:14: 3000000 INFO @ Tue, 16 Jun 2020 08:39:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:39:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:39:24: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:39:24: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:39:27: 5000000 INFO @ Tue, 16 Jun 2020 08:39:31: 1000000 INFO @ Tue, 16 Jun 2020 08:39:34: 6000000 INFO @ Tue, 16 Jun 2020 08:39:38: 2000000 INFO @ Tue, 16 Jun 2020 08:39:40: 7000000 INFO @ Tue, 16 Jun 2020 08:39:44: 3000000 INFO @ Tue, 16 Jun 2020 08:39:47: 8000000 INFO @ Tue, 16 Jun 2020 08:39:51: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:39:53: 9000000 INFO @ Tue, 16 Jun 2020 08:39:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:39:54: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:39:54: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:39:57: 5000000 INFO @ Tue, 16 Jun 2020 08:40:00: 10000000 INFO @ Tue, 16 Jun 2020 08:40:01: 1000000 INFO @ Tue, 16 Jun 2020 08:40:04: 6000000 INFO @ Tue, 16 Jun 2020 08:40:06: 11000000 INFO @ Tue, 16 Jun 2020 08:40:08: 2000000 INFO @ Tue, 16 Jun 2020 08:40:11: 7000000 INFO @ Tue, 16 Jun 2020 08:40:13: 12000000 INFO @ Tue, 16 Jun 2020 08:40:15: 3000000 INFO @ Tue, 16 Jun 2020 08:40:17: 8000000 INFO @ Tue, 16 Jun 2020 08:40:19: 13000000 INFO @ Tue, 16 Jun 2020 08:40:22: 4000000 INFO @ Tue, 16 Jun 2020 08:40:24: 9000000 INFO @ Tue, 16 Jun 2020 08:40:26: 14000000 INFO @ Tue, 16 Jun 2020 08:40:28: 5000000 INFO @ Tue, 16 Jun 2020 08:40:30: 10000000 INFO @ Tue, 16 Jun 2020 08:40:32: 15000000 INFO @ Tue, 16 Jun 2020 08:40:35: 6000000 INFO @ Tue, 16 Jun 2020 08:40:36: 11000000 INFO @ Tue, 16 Jun 2020 08:40:38: 16000000 INFO @ Tue, 16 Jun 2020 08:40:41: 7000000 INFO @ Tue, 16 Jun 2020 08:40:43: 12000000 INFO @ Tue, 16 Jun 2020 08:40:44: 17000000 INFO @ Tue, 16 Jun 2020 08:40:47: 8000000 INFO @ Tue, 16 Jun 2020 08:40:49: 13000000 INFO @ Tue, 16 Jun 2020 08:40:51: 18000000 INFO @ Tue, 16 Jun 2020 08:40:54: 9000000 INFO @ Tue, 16 Jun 2020 08:40:55: 14000000 INFO @ Tue, 16 Jun 2020 08:40:57: 19000000 INFO @ Tue, 16 Jun 2020 08:41:00: 10000000 INFO @ Tue, 16 Jun 2020 08:41:01: 15000000 INFO @ Tue, 16 Jun 2020 08:41:03: 20000000 INFO @ Tue, 16 Jun 2020 08:41:06: 11000000 INFO @ Tue, 16 Jun 2020 08:41:08: 16000000 INFO @ Tue, 16 Jun 2020 08:41:09: 21000000 INFO @ Tue, 16 Jun 2020 08:41:13: 12000000 INFO @ Tue, 16 Jun 2020 08:41:14: 17000000 INFO @ Tue, 16 Jun 2020 08:41:15: 22000000 INFO @ Tue, 16 Jun 2020 08:41:19: 13000000 INFO @ Tue, 16 Jun 2020 08:41:20: 18000000 INFO @ Tue, 16 Jun 2020 08:41:22: 23000000 INFO @ Tue, 16 Jun 2020 08:41:25: 14000000 INFO @ Tue, 16 Jun 2020 08:41:26: 19000000 INFO @ Tue, 16 Jun 2020 08:41:28: 24000000 INFO @ Tue, 16 Jun 2020 08:41:31: 15000000 INFO @ Tue, 16 Jun 2020 08:41:33: 20000000 INFO @ Tue, 16 Jun 2020 08:41:34: 25000000 INFO @ Tue, 16 Jun 2020 08:41:38: 16000000 INFO @ Tue, 16 Jun 2020 08:41:39: 21000000 INFO @ Tue, 16 Jun 2020 08:41:40: 26000000 INFO @ Tue, 16 Jun 2020 08:41:44: 17000000 INFO @ Tue, 16 Jun 2020 08:41:45: 22000000 INFO @ Tue, 16 Jun 2020 08:41:46: 27000000 INFO @ Tue, 16 Jun 2020 08:41:50: 18000000 INFO @ Tue, 16 Jun 2020 08:41:52: 23000000 INFO @ Tue, 16 Jun 2020 08:41:53: 28000000 INFO @ Tue, 16 Jun 2020 08:41:57: 19000000 INFO @ Tue, 16 Jun 2020 08:41:58: 24000000 INFO @ Tue, 16 Jun 2020 08:41:59: 29000000 INFO @ Tue, 16 Jun 2020 08:42:03: 20000000 INFO @ Tue, 16 Jun 2020 08:42:04: 25000000 INFO @ Tue, 16 Jun 2020 08:42:05: 30000000 INFO @ Tue, 16 Jun 2020 08:42:09: 21000000 INFO @ Tue, 16 Jun 2020 08:42:10: 26000000 INFO @ Tue, 16 Jun 2020 08:42:11: 31000000 INFO @ Tue, 16 Jun 2020 08:42:16: 22000000 INFO @ Tue, 16 Jun 2020 08:42:17: 27000000 INFO @ Tue, 16 Jun 2020 08:42:17: 32000000 INFO @ Tue, 16 Jun 2020 08:42:22: 23000000 INFO @ Tue, 16 Jun 2020 08:42:23: 28000000 INFO @ Tue, 16 Jun 2020 08:42:23: 33000000 INFO @ Tue, 16 Jun 2020 08:42:28: 24000000 INFO @ Tue, 16 Jun 2020 08:42:29: 29000000 INFO @ Tue, 16 Jun 2020 08:42:30: 34000000 INFO @ Tue, 16 Jun 2020 08:42:34: 25000000 INFO @ Tue, 16 Jun 2020 08:42:35: 30000000 INFO @ Tue, 16 Jun 2020 08:42:36: 35000000 INFO @ Tue, 16 Jun 2020 08:42:41: 26000000 INFO @ Tue, 16 Jun 2020 08:42:42: 31000000 INFO @ Tue, 16 Jun 2020 08:42:42: 36000000 INFO @ Tue, 16 Jun 2020 08:42:47: 27000000 INFO @ Tue, 16 Jun 2020 08:42:48: 32000000 INFO @ Tue, 16 Jun 2020 08:42:49: 37000000 INFO @ Tue, 16 Jun 2020 08:42:53: 28000000 INFO @ Tue, 16 Jun 2020 08:42:54: 33000000 INFO @ Tue, 16 Jun 2020 08:42:55: 38000000 INFO @ Tue, 16 Jun 2020 08:42:59: 29000000 INFO @ Tue, 16 Jun 2020 08:43:00: 34000000 INFO @ Tue, 16 Jun 2020 08:43:01: 39000000 INFO @ Tue, 16 Jun 2020 08:43:06: 30000000 INFO @ Tue, 16 Jun 2020 08:43:07: 35000000 INFO @ Tue, 16 Jun 2020 08:43:08: 40000000 INFO @ Tue, 16 Jun 2020 08:43:12: 31000000 INFO @ Tue, 16 Jun 2020 08:43:13: 36000000 INFO @ Tue, 16 Jun 2020 08:43:14: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:43:18: 32000000 INFO @ Tue, 16 Jun 2020 08:43:20: 37000000 INFO @ Tue, 16 Jun 2020 08:43:20: 42000000 INFO @ Tue, 16 Jun 2020 08:43:24: 33000000 INFO @ Tue, 16 Jun 2020 08:43:26: 43000000 INFO @ Tue, 16 Jun 2020 08:43:26: 38000000 INFO @ Tue, 16 Jun 2020 08:43:31: 34000000 INFO @ Tue, 16 Jun 2020 08:43:32: 44000000 INFO @ Tue, 16 Jun 2020 08:43:32: 39000000 INFO @ Tue, 16 Jun 2020 08:43:37: 35000000 INFO @ Tue, 16 Jun 2020 08:43:39: 40000000 INFO @ Tue, 16 Jun 2020 08:43:39: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:43:39: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:43:39: #1 total tags in treatment: 44995562 INFO @ Tue, 16 Jun 2020 08:43:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:43:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:43:40: #1 tags after filtering in treatment: 44995562 INFO @ Tue, 16 Jun 2020 08:43:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:43:40: #1 finished! INFO @ Tue, 16 Jun 2020 08:43:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:43:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:43:43: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:43:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:43:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:43:43: 36000000 INFO @ Tue, 16 Jun 2020 08:43:44: 41000000 INFO @ Tue, 16 Jun 2020 08:43:49: 37000000 INFO @ Tue, 16 Jun 2020 08:43:50: 42000000 INFO @ Tue, 16 Jun 2020 08:43:55: 38000000 INFO @ Tue, 16 Jun 2020 08:43:56: 43000000 INFO @ Tue, 16 Jun 2020 08:44:00: 39000000 INFO @ Tue, 16 Jun 2020 08:44:01: 44000000 INFO @ Tue, 16 Jun 2020 08:44:06: 40000000 INFO @ Tue, 16 Jun 2020 08:44:07: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:44:07: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:44:07: #1 total tags in treatment: 44995562 INFO @ Tue, 16 Jun 2020 08:44:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:44:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:44:08: #1 tags after filtering in treatment: 44995562 INFO @ Tue, 16 Jun 2020 08:44:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:44:08: #1 finished! INFO @ Tue, 16 Jun 2020 08:44:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:44:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:44:11: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:44:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:44:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:44:11: 41000000 INFO @ Tue, 16 Jun 2020 08:44:16: 42000000 INFO @ Tue, 16 Jun 2020 08:44:22: 43000000 INFO @ Tue, 16 Jun 2020 08:44:27: 44000000 INFO @ Tue, 16 Jun 2020 08:44:32: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:44:32: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:44:32: #1 total tags in treatment: 44995562 INFO @ Tue, 16 Jun 2020 08:44:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:44:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:44:33: #1 tags after filtering in treatment: 44995562 INFO @ Tue, 16 Jun 2020 08:44:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:44:33: #1 finished! INFO @ Tue, 16 Jun 2020 08:44:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:44:36: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:44:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:44:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1674089/SRX1674089.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。