Job ID = 16436965 SRX = SRX15684318 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:36:42 47188732 reads; of these: 47188732 (100.00%) were paired; of these: 3651661 (7.74%) aligned concordantly 0 times 36697475 (77.77%) aligned concordantly exactly 1 time 6839596 (14.49%) aligned concordantly >1 times ---- 3651661 pairs aligned concordantly 0 times; of these: 782936 (21.44%) aligned discordantly 1 time ---- 2868725 pairs aligned 0 times concordantly or discordantly; of these: 5737450 mates make up the pairs; of these: 4307229 (75.07%) aligned 0 times 822195 (14.33%) aligned exactly 1 time 608026 (10.60%) aligned >1 times 95.44% overall alignment rate Time searching: 01:36:42 Overall time: 01:36:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 68 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8409260 / 44186601 = 0.1903 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:23:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:23:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:23:36: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:23:43: 1000000 INFO @ Tue, 02 Aug 2022 14:23:50: 2000000 INFO @ Tue, 02 Aug 2022 14:23:56: 3000000 INFO @ Tue, 02 Aug 2022 14:24:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:24:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:24:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:24:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:24:10: 5000000 INFO @ Tue, 02 Aug 2022 14:24:13: 1000000 INFO @ Tue, 02 Aug 2022 14:24:16: 6000000 INFO @ Tue, 02 Aug 2022 14:24:19: 2000000 INFO @ Tue, 02 Aug 2022 14:24:23: 7000000 INFO @ Tue, 02 Aug 2022 14:24:26: 3000000 INFO @ Tue, 02 Aug 2022 14:24:30: 8000000 INFO @ Tue, 02 Aug 2022 14:24:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:24:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:24:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:24:36: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:24:37: 9000000 INFO @ Tue, 02 Aug 2022 14:24:39: 5000000 INFO @ Tue, 02 Aug 2022 14:24:44: 10000000 INFO @ Tue, 02 Aug 2022 14:24:45: 1000000 INFO @ Tue, 02 Aug 2022 14:24:46: 6000000 INFO @ Tue, 02 Aug 2022 14:24:51: 11000000 INFO @ Tue, 02 Aug 2022 14:24:53: 7000000 INFO @ Tue, 02 Aug 2022 14:24:53: 2000000 INFO @ Tue, 02 Aug 2022 14:24:57: 12000000 INFO @ Tue, 02 Aug 2022 14:24:59: 8000000 INFO @ Tue, 02 Aug 2022 14:25:01: 3000000 INFO @ Tue, 02 Aug 2022 14:25:04: 13000000 INFO @ Tue, 02 Aug 2022 14:25:06: 9000000 INFO @ Tue, 02 Aug 2022 14:25:09: 4000000 INFO @ Tue, 02 Aug 2022 14:25:11: 14000000 INFO @ Tue, 02 Aug 2022 14:25:12: 10000000 INFO @ Tue, 02 Aug 2022 14:25:17: 5000000 INFO @ Tue, 02 Aug 2022 14:25:18: 15000000 INFO @ Tue, 02 Aug 2022 14:25:19: 11000000 INFO @ Tue, 02 Aug 2022 14:25:25: 16000000 INFO @ Tue, 02 Aug 2022 14:25:25: 6000000 INFO @ Tue, 02 Aug 2022 14:25:26: 12000000 INFO @ Tue, 02 Aug 2022 14:25:31: 17000000 INFO @ Tue, 02 Aug 2022 14:25:33: 13000000 INFO @ Tue, 02 Aug 2022 14:25:33: 7000000 INFO @ Tue, 02 Aug 2022 14:25:38: 18000000 INFO @ Tue, 02 Aug 2022 14:25:39: 14000000 INFO @ Tue, 02 Aug 2022 14:25:41: 8000000 INFO @ Tue, 02 Aug 2022 14:25:45: 19000000 INFO @ Tue, 02 Aug 2022 14:25:46: 15000000 INFO @ Tue, 02 Aug 2022 14:25:49: 9000000 INFO @ Tue, 02 Aug 2022 14:25:52: 20000000 INFO @ Tue, 02 Aug 2022 14:25:52: 16000000 INFO @ Tue, 02 Aug 2022 14:25:57: 10000000 INFO @ Tue, 02 Aug 2022 14:25:59: 17000000 INFO @ Tue, 02 Aug 2022 14:25:59: 21000000 INFO @ Tue, 02 Aug 2022 14:26:05: 11000000 INFO @ Tue, 02 Aug 2022 14:26:06: 18000000 INFO @ Tue, 02 Aug 2022 14:26:06: 22000000 INFO @ Tue, 02 Aug 2022 14:26:12: 19000000 INFO @ Tue, 02 Aug 2022 14:26:13: 12000000 INFO @ Tue, 02 Aug 2022 14:26:13: 23000000 INFO @ Tue, 02 Aug 2022 14:26:19: 20000000 INFO @ Tue, 02 Aug 2022 14:26:20: 24000000 INFO @ Tue, 02 Aug 2022 14:26:20: 13000000 INFO @ Tue, 02 Aug 2022 14:26:25: 21000000 INFO @ Tue, 02 Aug 2022 14:26:27: 25000000 INFO @ Tue, 02 Aug 2022 14:26:28: 14000000 INFO @ Tue, 02 Aug 2022 14:26:32: 22000000 INFO @ Tue, 02 Aug 2022 14:26:34: 26000000 INFO @ Tue, 02 Aug 2022 14:26:36: 15000000 INFO @ Tue, 02 Aug 2022 14:26:39: 23000000 INFO @ Tue, 02 Aug 2022 14:26:41: 27000000 INFO @ Tue, 02 Aug 2022 14:26:44: 16000000 INFO @ Tue, 02 Aug 2022 14:26:45: 24000000 INFO @ Tue, 02 Aug 2022 14:26:48: 28000000 INFO @ Tue, 02 Aug 2022 14:26:52: 17000000 INFO @ Tue, 02 Aug 2022 14:26:52: 25000000 INFO @ Tue, 02 Aug 2022 14:26:55: 29000000 INFO @ Tue, 02 Aug 2022 14:26:59: 26000000 INFO @ Tue, 02 Aug 2022 14:26:59: 18000000 INFO @ Tue, 02 Aug 2022 14:27:02: 30000000 INFO @ Tue, 02 Aug 2022 14:27:05: 27000000 INFO @ Tue, 02 Aug 2022 14:27:07: 19000000 INFO @ Tue, 02 Aug 2022 14:27:10: 31000000 INFO @ Tue, 02 Aug 2022 14:27:12: 28000000 INFO @ Tue, 02 Aug 2022 14:27:15: 20000000 INFO @ Tue, 02 Aug 2022 14:27:17: 32000000 INFO @ Tue, 02 Aug 2022 14:27:20: 29000000 INFO @ Tue, 02 Aug 2022 14:27:23: 21000000 INFO @ Tue, 02 Aug 2022 14:27:24: 33000000 INFO @ Tue, 02 Aug 2022 14:27:27: 30000000 INFO @ Tue, 02 Aug 2022 14:27:31: 34000000 INFO @ Tue, 02 Aug 2022 14:27:31: 22000000 INFO @ Tue, 02 Aug 2022 14:27:34: 31000000 INFO @ Tue, 02 Aug 2022 14:27:38: 35000000 INFO @ Tue, 02 Aug 2022 14:27:39: 23000000 INFO @ Tue, 02 Aug 2022 14:27:41: 32000000 INFO @ Tue, 02 Aug 2022 14:27:45: 36000000 INFO @ Tue, 02 Aug 2022 14:27:47: 24000000 INFO @ Tue, 02 Aug 2022 14:27:48: 33000000 INFO @ Tue, 02 Aug 2022 14:27:52: 37000000 INFO @ Tue, 02 Aug 2022 14:27:54: 25000000 INFO @ Tue, 02 Aug 2022 14:27:55: 34000000 INFO @ Tue, 02 Aug 2022 14:27:59: 38000000 INFO @ Tue, 02 Aug 2022 14:28:02: 35000000 INFO @ Tue, 02 Aug 2022 14:28:02: 26000000 INFO @ Tue, 02 Aug 2022 14:28:06: 39000000 INFO @ Tue, 02 Aug 2022 14:28:09: 36000000 INFO @ Tue, 02 Aug 2022 14:28:10: 27000000 INFO @ Tue, 02 Aug 2022 14:28:13: 40000000 INFO @ Tue, 02 Aug 2022 14:28:16: 37000000 INFO @ Tue, 02 Aug 2022 14:28:18: 28000000 INFO @ Tue, 02 Aug 2022 14:28:20: 41000000 INFO @ Tue, 02 Aug 2022 14:28:23: 38000000 INFO @ Tue, 02 Aug 2022 14:28:26: 29000000 INFO @ Tue, 02 Aug 2022 14:28:27: 42000000 INFO @ Tue, 02 Aug 2022 14:28:30: 39000000 INFO @ Tue, 02 Aug 2022 14:28:33: 30000000 INFO @ Tue, 02 Aug 2022 14:28:34: 43000000 INFO @ Tue, 02 Aug 2022 14:28:37: 40000000 INFO @ Tue, 02 Aug 2022 14:28:40: 44000000 INFO @ Tue, 02 Aug 2022 14:28:41: 31000000 INFO @ Tue, 02 Aug 2022 14:28:44: 41000000 INFO @ Tue, 02 Aug 2022 14:28:47: 45000000 INFO @ Tue, 02 Aug 2022 14:28:48: 32000000 INFO @ Tue, 02 Aug 2022 14:28:51: 42000000 INFO @ Tue, 02 Aug 2022 14:28:54: 46000000 INFO @ Tue, 02 Aug 2022 14:28:56: 33000000 INFO @ Tue, 02 Aug 2022 14:28:58: 43000000 INFO @ Tue, 02 Aug 2022 14:29:01: 47000000 INFO @ Tue, 02 Aug 2022 14:29:03: 34000000 INFO @ Tue, 02 Aug 2022 14:29:05: 44000000 INFO @ Tue, 02 Aug 2022 14:29:08: 48000000 INFO @ Tue, 02 Aug 2022 14:29:11: 35000000 INFO @ Tue, 02 Aug 2022 14:29:12: 45000000 INFO @ Tue, 02 Aug 2022 14:29:15: 49000000 INFO @ Tue, 02 Aug 2022 14:29:18: 36000000 INFO @ Tue, 02 Aug 2022 14:29:19: 46000000 INFO @ Tue, 02 Aug 2022 14:29:22: 50000000 INFO @ Tue, 02 Aug 2022 14:29:25: 37000000 INFO @ Tue, 02 Aug 2022 14:29:25: 47000000 INFO @ Tue, 02 Aug 2022 14:29:29: 51000000 INFO @ Tue, 02 Aug 2022 14:29:32: 48000000 INFO @ Tue, 02 Aug 2022 14:29:33: 38000000 INFO @ Tue, 02 Aug 2022 14:29:35: 52000000 INFO @ Tue, 02 Aug 2022 14:29:39: 49000000 INFO @ Tue, 02 Aug 2022 14:29:41: 39000000 INFO @ Tue, 02 Aug 2022 14:29:42: 53000000 INFO @ Tue, 02 Aug 2022 14:29:46: 50000000 INFO @ Tue, 02 Aug 2022 14:29:48: 40000000 INFO @ Tue, 02 Aug 2022 14:29:49: 54000000 INFO @ Tue, 02 Aug 2022 14:29:53: 51000000 INFO @ Tue, 02 Aug 2022 14:29:55: 41000000 INFO @ Tue, 02 Aug 2022 14:29:56: 55000000 INFO @ Tue, 02 Aug 2022 14:30:00: 52000000 INFO @ Tue, 02 Aug 2022 14:30:03: 42000000 INFO @ Tue, 02 Aug 2022 14:30:03: 56000000 INFO @ Tue, 02 Aug 2022 14:30:07: 53000000 INFO @ Tue, 02 Aug 2022 14:30:10: 57000000 INFO @ Tue, 02 Aug 2022 14:30:10: 43000000 INFO @ Tue, 02 Aug 2022 14:30:14: 54000000 INFO @ Tue, 02 Aug 2022 14:30:17: 58000000 INFO @ Tue, 02 Aug 2022 14:30:18: 44000000 INFO @ Tue, 02 Aug 2022 14:30:20: 55000000 INFO @ Tue, 02 Aug 2022 14:30:23: 59000000 INFO @ Tue, 02 Aug 2022 14:30:25: 45000000 INFO @ Tue, 02 Aug 2022 14:30:27: 56000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:30:30: 60000000 INFO @ Tue, 02 Aug 2022 14:30:33: 46000000 INFO @ Tue, 02 Aug 2022 14:30:34: 57000000 INFO @ Tue, 02 Aug 2022 14:30:37: 61000000 INFO @ Tue, 02 Aug 2022 14:30:40: 47000000 INFO @ Tue, 02 Aug 2022 14:30:41: 58000000 INFO @ Tue, 02 Aug 2022 14:30:44: 62000000 INFO @ Tue, 02 Aug 2022 14:30:47: 48000000 INFO @ Tue, 02 Aug 2022 14:30:48: 59000000 INFO @ Tue, 02 Aug 2022 14:30:51: 63000000 INFO @ Tue, 02 Aug 2022 14:30:55: 60000000 INFO @ Tue, 02 Aug 2022 14:30:55: 49000000 INFO @ Tue, 02 Aug 2022 14:30:58: 64000000 INFO @ Tue, 02 Aug 2022 14:31:02: 61000000 INFO @ Tue, 02 Aug 2022 14:31:03: 50000000 INFO @ Tue, 02 Aug 2022 14:31:05: 65000000 INFO @ Tue, 02 Aug 2022 14:31:09: 62000000 INFO @ Tue, 02 Aug 2022 14:31:10: 51000000 INFO @ Tue, 02 Aug 2022 14:31:12: 66000000 INFO @ Tue, 02 Aug 2022 14:31:16: 63000000 INFO @ Tue, 02 Aug 2022 14:31:17: 52000000 INFO @ Tue, 02 Aug 2022 14:31:18: 67000000 INFO @ Tue, 02 Aug 2022 14:31:23: 64000000 INFO @ Tue, 02 Aug 2022 14:31:25: 53000000 INFO @ Tue, 02 Aug 2022 14:31:25: 68000000 INFO @ Tue, 02 Aug 2022 14:31:30: 65000000 INFO @ Tue, 02 Aug 2022 14:31:32: 69000000 INFO @ Tue, 02 Aug 2022 14:31:33: 54000000 INFO @ Tue, 02 Aug 2022 14:31:37: 66000000 INFO @ Tue, 02 Aug 2022 14:31:40: 70000000 INFO @ Tue, 02 Aug 2022 14:31:40: 55000000 INFO @ Tue, 02 Aug 2022 14:31:43: 67000000 INFO @ Tue, 02 Aug 2022 14:31:47: 71000000 INFO @ Tue, 02 Aug 2022 14:31:48: 56000000 INFO @ Tue, 02 Aug 2022 14:31:50: 68000000 INFO @ Tue, 02 Aug 2022 14:31:54: 72000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:31:55: 57000000 INFO @ Tue, 02 Aug 2022 14:31:57: 69000000 INFO @ Tue, 02 Aug 2022 14:32:01: 73000000 INFO @ Tue, 02 Aug 2022 14:32:03: 58000000 INFO @ Tue, 02 Aug 2022 14:32:03: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:32:03: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:32:03: #1 total tags in treatment: 35212900 INFO @ Tue, 02 Aug 2022 14:32:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:32:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:32:03: #1 tags after filtering in treatment: 30227377 INFO @ Tue, 02 Aug 2022 14:32:03: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 14:32:03: #1 finished! INFO @ Tue, 02 Aug 2022 14:32:03: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:32:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:32:04: 70000000 INFO @ Tue, 02 Aug 2022 14:32:05: #2 number of paired peaks: 138 WARNING @ Tue, 02 Aug 2022 14:32:05: Fewer paired peaks (138) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 138 pairs to build model! INFO @ Tue, 02 Aug 2022 14:32:05: start model_add_line... INFO @ Tue, 02 Aug 2022 14:32:06: start X-correlation... INFO @ Tue, 02 Aug 2022 14:32:06: end of X-cor INFO @ Tue, 02 Aug 2022 14:32:06: #2 finished! INFO @ Tue, 02 Aug 2022 14:32:06: #2 predicted fragment length is 152 bps INFO @ Tue, 02 Aug 2022 14:32:06: #2 alternative fragment length(s) may be 2,115,133,152 bps INFO @ Tue, 02 Aug 2022 14:32:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.05_model.r WARNING @ Tue, 02 Aug 2022 14:32:06: #2 Since the d (152) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:32:06: #2 You may need to consider one of the other alternative d(s): 2,115,133,152 WARNING @ Tue, 02 Aug 2022 14:32:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:32:06: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:32:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:32:10: 59000000 INFO @ Tue, 02 Aug 2022 14:32:11: 71000000 INFO @ Tue, 02 Aug 2022 14:32:18: 60000000 INFO @ Tue, 02 Aug 2022 14:32:18: 72000000 INFO @ Tue, 02 Aug 2022 14:32:25: 73000000 INFO @ Tue, 02 Aug 2022 14:32:25: 61000000 INFO @ Tue, 02 Aug 2022 14:32:27: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:32:27: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:32:27: #1 total tags in treatment: 35212900 INFO @ Tue, 02 Aug 2022 14:32:27: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:32:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:32:27: #1 tags after filtering in treatment: 30227377 INFO @ Tue, 02 Aug 2022 14:32:27: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 14:32:27: #1 finished! INFO @ Tue, 02 Aug 2022 14:32:27: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:32:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:32:29: #2 number of paired peaks: 138 WARNING @ Tue, 02 Aug 2022 14:32:29: Fewer paired peaks (138) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 138 pairs to build model! INFO @ Tue, 02 Aug 2022 14:32:29: start model_add_line... INFO @ Tue, 02 Aug 2022 14:32:29: start X-correlation... INFO @ Tue, 02 Aug 2022 14:32:29: end of X-cor INFO @ Tue, 02 Aug 2022 14:32:29: #2 finished! INFO @ Tue, 02 Aug 2022 14:32:29: #2 predicted fragment length is 152 bps INFO @ Tue, 02 Aug 2022 14:32:29: #2 alternative fragment length(s) may be 2,115,133,152 bps INFO @ Tue, 02 Aug 2022 14:32:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.10_model.r WARNING @ Tue, 02 Aug 2022 14:32:29: #2 Since the d (152) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:32:29: #2 You may need to consider one of the other alternative d(s): 2,115,133,152 WARNING @ Tue, 02 Aug 2022 14:32:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:32:29: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:32:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:32:32: 62000000 INFO @ Tue, 02 Aug 2022 14:32:40: 63000000 INFO @ Tue, 02 Aug 2022 14:32:47: 64000000 INFO @ Tue, 02 Aug 2022 14:32:52: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:32:54: 65000000 INFO @ Tue, 02 Aug 2022 14:33:01: 66000000 INFO @ Tue, 02 Aug 2022 14:33:08: 67000000 INFO @ Tue, 02 Aug 2022 14:33:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:33:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:33:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.05_summits.bed INFO @ Tue, 02 Aug 2022 14:33:13: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (758 records, 4 fields): 29 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:33:15: 68000000 INFO @ Tue, 02 Aug 2022 14:33:16: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:33:23: 69000000 INFO @ Tue, 02 Aug 2022 14:33:30: 70000000 INFO @ Tue, 02 Aug 2022 14:33:37: 71000000 INFO @ Tue, 02 Aug 2022 14:33:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:33:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:33:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.10_summits.bed INFO @ Tue, 02 Aug 2022 14:33:38: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (452 records, 4 fields): 25 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:33:45: 72000000 INFO @ Tue, 02 Aug 2022 14:33:52: 73000000 INFO @ Tue, 02 Aug 2022 14:33:54: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:33:54: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:33:54: #1 total tags in treatment: 35212900 INFO @ Tue, 02 Aug 2022 14:33:54: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:33:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:33:55: #1 tags after filtering in treatment: 30227377 INFO @ Tue, 02 Aug 2022 14:33:55: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 14:33:55: #1 finished! INFO @ Tue, 02 Aug 2022 14:33:55: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:33:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:33:56: #2 number of paired peaks: 138 WARNING @ Tue, 02 Aug 2022 14:33:56: Fewer paired peaks (138) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 138 pairs to build model! INFO @ Tue, 02 Aug 2022 14:33:56: start model_add_line... INFO @ Tue, 02 Aug 2022 14:33:57: start X-correlation... INFO @ Tue, 02 Aug 2022 14:33:57: end of X-cor INFO @ Tue, 02 Aug 2022 14:33:57: #2 finished! INFO @ Tue, 02 Aug 2022 14:33:57: #2 predicted fragment length is 152 bps INFO @ Tue, 02 Aug 2022 14:33:57: #2 alternative fragment length(s) may be 2,115,133,152 bps INFO @ Tue, 02 Aug 2022 14:33:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.20_model.r WARNING @ Tue, 02 Aug 2022 14:33:57: #2 Since the d (152) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:33:57: #2 You may need to consider one of the other alternative d(s): 2,115,133,152 WARNING @ Tue, 02 Aug 2022 14:33:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:33:57: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:33:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:34:42: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:35:03: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:35:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:35:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX15684318/SRX15684318.20_summits.bed INFO @ Tue, 02 Aug 2022 14:35:03: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (308 records, 4 fields): 22 millis CompletedMACS2peakCalling