Job ID = 16436974 SRX = SRX15684316 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:36:14 46995272 reads; of these: 46995272 (100.00%) were paired; of these: 8667453 (18.44%) aligned concordantly 0 times 30472336 (64.84%) aligned concordantly exactly 1 time 7855483 (16.72%) aligned concordantly >1 times ---- 8667453 pairs aligned concordantly 0 times; of these: 886391 (10.23%) aligned discordantly 1 time ---- 7781062 pairs aligned 0 times concordantly or discordantly; of these: 15562124 mates make up the pairs; of these: 14313904 (91.98%) aligned 0 times 645519 (4.15%) aligned exactly 1 time 602701 (3.87%) aligned >1 times 84.77% overall alignment rate Time searching: 01:36:14 Overall time: 01:36:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 7671977 / 39087841 = 0.1963 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:20:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:20:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:20:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:20:44: 1000000 INFO @ Tue, 02 Aug 2022 14:20:50: 2000000 INFO @ Tue, 02 Aug 2022 14:20:56: 3000000 INFO @ Tue, 02 Aug 2022 14:21:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:21:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:21:06: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:21:06: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:21:09: 5000000 INFO @ Tue, 02 Aug 2022 14:21:13: 1000000 INFO @ Tue, 02 Aug 2022 14:21:16: 6000000 INFO @ Tue, 02 Aug 2022 14:21:20: 2000000 INFO @ Tue, 02 Aug 2022 14:21:24: 7000000 INFO @ Tue, 02 Aug 2022 14:21:28: 3000000 INFO @ Tue, 02 Aug 2022 14:21:31: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:21:35: 4000000 INFO @ Tue, 02 Aug 2022 14:21:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:21:37: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:21:37: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:21:38: 9000000 INFO @ Tue, 02 Aug 2022 14:21:42: 5000000 INFO @ Tue, 02 Aug 2022 14:21:45: 10000000 INFO @ Tue, 02 Aug 2022 14:21:46: 1000000 INFO @ Tue, 02 Aug 2022 14:21:49: 6000000 INFO @ Tue, 02 Aug 2022 14:21:52: 11000000 INFO @ Tue, 02 Aug 2022 14:21:54: 2000000 INFO @ Tue, 02 Aug 2022 14:21:56: 7000000 INFO @ Tue, 02 Aug 2022 14:22:00: 12000000 INFO @ Tue, 02 Aug 2022 14:22:02: 3000000 INFO @ Tue, 02 Aug 2022 14:22:03: 8000000 INFO @ Tue, 02 Aug 2022 14:22:07: 13000000 INFO @ Tue, 02 Aug 2022 14:22:10: 9000000 INFO @ Tue, 02 Aug 2022 14:22:11: 4000000 INFO @ Tue, 02 Aug 2022 14:22:14: 14000000 INFO @ Tue, 02 Aug 2022 14:22:17: 10000000 INFO @ Tue, 02 Aug 2022 14:22:19: 5000000 INFO @ Tue, 02 Aug 2022 14:22:21: 15000000 INFO @ Tue, 02 Aug 2022 14:22:24: 11000000 INFO @ Tue, 02 Aug 2022 14:22:28: 6000000 INFO @ Tue, 02 Aug 2022 14:22:29: 16000000 INFO @ Tue, 02 Aug 2022 14:22:31: 12000000 INFO @ Tue, 02 Aug 2022 14:22:36: 7000000 INFO @ Tue, 02 Aug 2022 14:22:36: 17000000 INFO @ Tue, 02 Aug 2022 14:22:38: 13000000 INFO @ Tue, 02 Aug 2022 14:22:44: 18000000 INFO @ Tue, 02 Aug 2022 14:22:45: 8000000 INFO @ Tue, 02 Aug 2022 14:22:45: 14000000 INFO @ Tue, 02 Aug 2022 14:22:51: 19000000 INFO @ Tue, 02 Aug 2022 14:22:52: 15000000 INFO @ Tue, 02 Aug 2022 14:22:53: 9000000 INFO @ Tue, 02 Aug 2022 14:22:58: 20000000 INFO @ Tue, 02 Aug 2022 14:22:59: 16000000 INFO @ Tue, 02 Aug 2022 14:23:01: 10000000 INFO @ Tue, 02 Aug 2022 14:23:06: 17000000 INFO @ Tue, 02 Aug 2022 14:23:06: 21000000 INFO @ Tue, 02 Aug 2022 14:23:10: 11000000 INFO @ Tue, 02 Aug 2022 14:23:13: 18000000 INFO @ Tue, 02 Aug 2022 14:23:13: 22000000 INFO @ Tue, 02 Aug 2022 14:23:18: 12000000 INFO @ Tue, 02 Aug 2022 14:23:20: 19000000 INFO @ Tue, 02 Aug 2022 14:23:21: 23000000 INFO @ Tue, 02 Aug 2022 14:23:26: 13000000 INFO @ Tue, 02 Aug 2022 14:23:27: 20000000 INFO @ Tue, 02 Aug 2022 14:23:28: 24000000 INFO @ Tue, 02 Aug 2022 14:23:34: 21000000 INFO @ Tue, 02 Aug 2022 14:23:34: 14000000 INFO @ Tue, 02 Aug 2022 14:23:35: 25000000 INFO @ Tue, 02 Aug 2022 14:23:41: 22000000 INFO @ Tue, 02 Aug 2022 14:23:42: 26000000 INFO @ Tue, 02 Aug 2022 14:23:42: 15000000 INFO @ Tue, 02 Aug 2022 14:23:48: 23000000 INFO @ Tue, 02 Aug 2022 14:23:49: 27000000 INFO @ Tue, 02 Aug 2022 14:23:50: 16000000 INFO @ Tue, 02 Aug 2022 14:23:55: 24000000 INFO @ Tue, 02 Aug 2022 14:23:56: 28000000 INFO @ Tue, 02 Aug 2022 14:23:59: 17000000 INFO @ Tue, 02 Aug 2022 14:24:02: 25000000 INFO @ Tue, 02 Aug 2022 14:24:03: 29000000 INFO @ Tue, 02 Aug 2022 14:24:07: 18000000 INFO @ Tue, 02 Aug 2022 14:24:10: 26000000 INFO @ Tue, 02 Aug 2022 14:24:11: 30000000 INFO @ Tue, 02 Aug 2022 14:24:15: 19000000 INFO @ Tue, 02 Aug 2022 14:24:17: 27000000 INFO @ Tue, 02 Aug 2022 14:24:18: 31000000 INFO @ Tue, 02 Aug 2022 14:24:23: 20000000 INFO @ Tue, 02 Aug 2022 14:24:24: 28000000 INFO @ Tue, 02 Aug 2022 14:24:25: 32000000 INFO @ Tue, 02 Aug 2022 14:24:31: 29000000 INFO @ Tue, 02 Aug 2022 14:24:32: 21000000 INFO @ Tue, 02 Aug 2022 14:24:32: 33000000 INFO @ Tue, 02 Aug 2022 14:24:38: 30000000 INFO @ Tue, 02 Aug 2022 14:24:39: 34000000 INFO @ Tue, 02 Aug 2022 14:24:40: 22000000 INFO @ Tue, 02 Aug 2022 14:24:45: 31000000 INFO @ Tue, 02 Aug 2022 14:24:46: 35000000 INFO @ Tue, 02 Aug 2022 14:24:49: 23000000 INFO @ Tue, 02 Aug 2022 14:24:52: 32000000 INFO @ Tue, 02 Aug 2022 14:24:54: 36000000 INFO @ Tue, 02 Aug 2022 14:24:58: 24000000 INFO @ Tue, 02 Aug 2022 14:25:00: 33000000 INFO @ Tue, 02 Aug 2022 14:25:01: 37000000 INFO @ Tue, 02 Aug 2022 14:25:06: 25000000 INFO @ Tue, 02 Aug 2022 14:25:07: 34000000 INFO @ Tue, 02 Aug 2022 14:25:08: 38000000 INFO @ Tue, 02 Aug 2022 14:25:14: 35000000 INFO @ Tue, 02 Aug 2022 14:25:15: 26000000 INFO @ Tue, 02 Aug 2022 14:25:15: 39000000 INFO @ Tue, 02 Aug 2022 14:25:21: 36000000 INFO @ Tue, 02 Aug 2022 14:25:22: 40000000 INFO @ Tue, 02 Aug 2022 14:25:24: 27000000 INFO @ Tue, 02 Aug 2022 14:25:28: 37000000 INFO @ Tue, 02 Aug 2022 14:25:30: 41000000 INFO @ Tue, 02 Aug 2022 14:25:32: 28000000 INFO @ Tue, 02 Aug 2022 14:25:35: 38000000 INFO @ Tue, 02 Aug 2022 14:25:37: 42000000 INFO @ Tue, 02 Aug 2022 14:25:41: 29000000 INFO @ Tue, 02 Aug 2022 14:25:43: 39000000 INFO @ Tue, 02 Aug 2022 14:25:44: 43000000 INFO @ Tue, 02 Aug 2022 14:25:49: 30000000 INFO @ Tue, 02 Aug 2022 14:25:50: 40000000 INFO @ Tue, 02 Aug 2022 14:25:51: 44000000 INFO @ Tue, 02 Aug 2022 14:25:57: 41000000 INFO @ Tue, 02 Aug 2022 14:25:58: 31000000 INFO @ Tue, 02 Aug 2022 14:25:58: 45000000 INFO @ Tue, 02 Aug 2022 14:26:04: 42000000 INFO @ Tue, 02 Aug 2022 14:26:05: 46000000 INFO @ Tue, 02 Aug 2022 14:26:06: 32000000 INFO @ Tue, 02 Aug 2022 14:26:11: 43000000 INFO @ Tue, 02 Aug 2022 14:26:13: 47000000 INFO @ Tue, 02 Aug 2022 14:26:15: 33000000 INFO @ Tue, 02 Aug 2022 14:26:18: 44000000 INFO @ Tue, 02 Aug 2022 14:26:20: 48000000 INFO @ Tue, 02 Aug 2022 14:26:23: 34000000 INFO @ Tue, 02 Aug 2022 14:26:25: 45000000 INFO @ Tue, 02 Aug 2022 14:26:27: 49000000 INFO @ Tue, 02 Aug 2022 14:26:32: 35000000 INFO @ Tue, 02 Aug 2022 14:26:33: 46000000 INFO @ Tue, 02 Aug 2022 14:26:34: 50000000 INFO @ Tue, 02 Aug 2022 14:26:40: 47000000 INFO @ Tue, 02 Aug 2022 14:26:41: 51000000 INFO @ Tue, 02 Aug 2022 14:26:41: 36000000 INFO @ Tue, 02 Aug 2022 14:26:47: 48000000 INFO @ Tue, 02 Aug 2022 14:26:48: 52000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:26:50: 37000000 INFO @ Tue, 02 Aug 2022 14:26:54: 49000000 INFO @ Tue, 02 Aug 2022 14:26:55: 53000000 INFO @ Tue, 02 Aug 2022 14:26:58: 38000000 INFO @ Tue, 02 Aug 2022 14:27:01: 50000000 INFO @ Tue, 02 Aug 2022 14:27:03: 54000000 INFO @ Tue, 02 Aug 2022 14:27:07: 39000000 INFO @ Tue, 02 Aug 2022 14:27:08: 51000000 INFO @ Tue, 02 Aug 2022 14:27:10: 55000000 INFO @ Tue, 02 Aug 2022 14:27:15: 52000000 INFO @ Tue, 02 Aug 2022 14:27:16: 40000000 INFO @ Tue, 02 Aug 2022 14:27:17: 56000000 INFO @ Tue, 02 Aug 2022 14:27:22: 53000000 INFO @ Tue, 02 Aug 2022 14:27:24: 57000000 INFO @ Tue, 02 Aug 2022 14:27:25: 41000000 INFO @ Tue, 02 Aug 2022 14:27:30: 54000000 INFO @ Tue, 02 Aug 2022 14:27:31: 58000000 INFO @ Tue, 02 Aug 2022 14:27:33: 42000000 INFO @ Tue, 02 Aug 2022 14:27:37: 55000000 INFO @ Tue, 02 Aug 2022 14:27:38: 59000000 INFO @ Tue, 02 Aug 2022 14:27:42: 43000000 INFO @ Tue, 02 Aug 2022 14:27:44: 56000000 INFO @ Tue, 02 Aug 2022 14:27:46: 60000000 INFO @ Tue, 02 Aug 2022 14:27:51: 44000000 INFO @ Tue, 02 Aug 2022 14:27:51: 57000000 INFO @ Tue, 02 Aug 2022 14:27:53: 61000000 INFO @ Tue, 02 Aug 2022 14:27:58: 58000000 INFO @ Tue, 02 Aug 2022 14:27:59: 45000000 INFO @ Tue, 02 Aug 2022 14:28:00: 62000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:28:05: 59000000 INFO @ Tue, 02 Aug 2022 14:28:07: 63000000 INFO @ Tue, 02 Aug 2022 14:28:08: 46000000 INFO @ Tue, 02 Aug 2022 14:28:13: 60000000 INFO @ Tue, 02 Aug 2022 14:28:15: 64000000 INFO @ Tue, 02 Aug 2022 14:28:17: 47000000 INFO @ Tue, 02 Aug 2022 14:28:17: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:28:17: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:28:17: #1 total tags in treatment: 30752974 INFO @ Tue, 02 Aug 2022 14:28:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:28:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:28:18: #1 tags after filtering in treatment: 25558189 INFO @ Tue, 02 Aug 2022 14:28:18: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 02 Aug 2022 14:28:18: #1 finished! INFO @ Tue, 02 Aug 2022 14:28:18: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:28:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:28:19: #2 number of paired peaks: 250 WARNING @ Tue, 02 Aug 2022 14:28:19: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Tue, 02 Aug 2022 14:28:19: start model_add_line... INFO @ Tue, 02 Aug 2022 14:28:20: start X-correlation... INFO @ Tue, 02 Aug 2022 14:28:20: 61000000 INFO @ Tue, 02 Aug 2022 14:28:20: end of X-cor INFO @ Tue, 02 Aug 2022 14:28:20: #2 finished! INFO @ Tue, 02 Aug 2022 14:28:20: #2 predicted fragment length is 165 bps INFO @ Tue, 02 Aug 2022 14:28:20: #2 alternative fragment length(s) may be 2,165 bps INFO @ Tue, 02 Aug 2022 14:28:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.05_model.r WARNING @ Tue, 02 Aug 2022 14:28:20: #2 Since the d (165) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:28:20: #2 You may need to consider one of the other alternative d(s): 2,165 WARNING @ Tue, 02 Aug 2022 14:28:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:28:20: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:28:20: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:28:25: 48000000 INFO @ Tue, 02 Aug 2022 14:28:27: 62000000 INFO @ Tue, 02 Aug 2022 14:28:34: 63000000 INFO @ Tue, 02 Aug 2022 14:28:34: 49000000 INFO @ Tue, 02 Aug 2022 14:28:41: 64000000 INFO @ Tue, 02 Aug 2022 14:28:42: 50000000 INFO @ Tue, 02 Aug 2022 14:28:44: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:28:44: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:28:44: #1 total tags in treatment: 30752974 INFO @ Tue, 02 Aug 2022 14:28:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:28:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:28:44: #1 tags after filtering in treatment: 25558189 INFO @ Tue, 02 Aug 2022 14:28:44: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 02 Aug 2022 14:28:44: #1 finished! INFO @ Tue, 02 Aug 2022 14:28:44: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:28:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:28:46: #2 number of paired peaks: 250 WARNING @ Tue, 02 Aug 2022 14:28:46: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Tue, 02 Aug 2022 14:28:46: start model_add_line... INFO @ Tue, 02 Aug 2022 14:28:46: start X-correlation... INFO @ Tue, 02 Aug 2022 14:28:46: end of X-cor INFO @ Tue, 02 Aug 2022 14:28:46: #2 finished! INFO @ Tue, 02 Aug 2022 14:28:46: #2 predicted fragment length is 165 bps INFO @ Tue, 02 Aug 2022 14:28:46: #2 alternative fragment length(s) may be 2,165 bps INFO @ Tue, 02 Aug 2022 14:28:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.10_model.r WARNING @ Tue, 02 Aug 2022 14:28:46: #2 Since the d (165) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:28:46: #2 You may need to consider one of the other alternative d(s): 2,165 WARNING @ Tue, 02 Aug 2022 14:28:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:28:46: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:28:46: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:28:50: 51000000 INFO @ Tue, 02 Aug 2022 14:28:59: 52000000 INFO @ Tue, 02 Aug 2022 14:29:06: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:29:07: 53000000 INFO @ Tue, 02 Aug 2022 14:29:15: 54000000 INFO @ Tue, 02 Aug 2022 14:29:24: 55000000 INFO @ Tue, 02 Aug 2022 14:29:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:29:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:29:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.05_summits.bed INFO @ Tue, 02 Aug 2022 14:29:27: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2736 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:29:32: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:29:32: 56000000 INFO @ Tue, 02 Aug 2022 14:29:40: 57000000 INFO @ Tue, 02 Aug 2022 14:29:48: 58000000 INFO @ Tue, 02 Aug 2022 14:29:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:29:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:29:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.10_summits.bed INFO @ Tue, 02 Aug 2022 14:29:53: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (1205 records, 4 fields): 96 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:29:57: 59000000 INFO @ Tue, 02 Aug 2022 14:30:05: 60000000 INFO @ Tue, 02 Aug 2022 14:30:13: 61000000 INFO @ Tue, 02 Aug 2022 14:30:21: 62000000 INFO @ Tue, 02 Aug 2022 14:30:29: 63000000 INFO @ Tue, 02 Aug 2022 14:30:37: 64000000 INFO @ Tue, 02 Aug 2022 14:30:40: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:30:40: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:30:40: #1 total tags in treatment: 30752974 INFO @ Tue, 02 Aug 2022 14:30:40: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:30:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:30:40: #1 tags after filtering in treatment: 25558189 INFO @ Tue, 02 Aug 2022 14:30:40: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 02 Aug 2022 14:30:40: #1 finished! INFO @ Tue, 02 Aug 2022 14:30:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:30:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:30:42: #2 number of paired peaks: 250 WARNING @ Tue, 02 Aug 2022 14:30:42: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Tue, 02 Aug 2022 14:30:42: start model_add_line... INFO @ Tue, 02 Aug 2022 14:30:42: start X-correlation... INFO @ Tue, 02 Aug 2022 14:30:42: end of X-cor INFO @ Tue, 02 Aug 2022 14:30:42: #2 finished! INFO @ Tue, 02 Aug 2022 14:30:42: #2 predicted fragment length is 165 bps INFO @ Tue, 02 Aug 2022 14:30:42: #2 alternative fragment length(s) may be 2,165 bps INFO @ Tue, 02 Aug 2022 14:30:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.20_model.r WARNING @ Tue, 02 Aug 2022 14:30:42: #2 Since the d (165) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:30:42: #2 You may need to consider one of the other alternative d(s): 2,165 WARNING @ Tue, 02 Aug 2022 14:30:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:30:42: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:30:42: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:31:29: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:31:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:31:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:31:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX15684316/SRX15684316.20_summits.bed INFO @ Tue, 02 Aug 2022 14:31:49: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (492 records, 4 fields): 36 millis CompletedMACS2peakCalling