Job ID = 6366478 SRX = SRX147630 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:54:29 prefetch.2.10.7: 1) Downloading 'SRR496307'... 2020-06-15T22:54:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:55:05 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:55:05 prefetch.2.10.7: 'SRR496307' is valid 2020-06-15T22:55:05 prefetch.2.10.7: 1) 'SRR496307' was downloaded successfully Read 6036969 spots for SRR496307/SRR496307.sra Written 6036969 spots for SRR496307/SRR496307.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:37 6036969 reads; of these: 6036969 (100.00%) were unpaired; of these: 3446292 (57.09%) aligned 0 times 2151266 (35.63%) aligned exactly 1 time 439411 (7.28%) aligned >1 times 42.91% overall alignment rate Time searching: 00:00:37 Overall time: 00:00:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 399483 / 2590677 = 0.1542 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:57:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:57:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:57:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:57:07: 1000000 INFO @ Tue, 16 Jun 2020 07:57:13: 2000000 INFO @ Tue, 16 Jun 2020 07:57:14: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:57:14: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:57:14: #1 total tags in treatment: 2191194 INFO @ Tue, 16 Jun 2020 07:57:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:57:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:57:14: #1 tags after filtering in treatment: 2191194 INFO @ Tue, 16 Jun 2020 07:57:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:57:14: #1 finished! INFO @ Tue, 16 Jun 2020 07:57:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:57:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:57:14: #2 number of paired peaks: 492 WARNING @ Tue, 16 Jun 2020 07:57:14: Fewer paired peaks (492) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 492 pairs to build model! INFO @ Tue, 16 Jun 2020 07:57:14: start model_add_line... INFO @ Tue, 16 Jun 2020 07:57:14: start X-correlation... INFO @ Tue, 16 Jun 2020 07:57:14: end of X-cor INFO @ Tue, 16 Jun 2020 07:57:14: #2 finished! INFO @ Tue, 16 Jun 2020 07:57:14: #2 predicted fragment length is 86 bps INFO @ Tue, 16 Jun 2020 07:57:14: #2 alternative fragment length(s) may be 39,86 bps INFO @ Tue, 16 Jun 2020 07:57:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.05_model.r INFO @ Tue, 16 Jun 2020 07:57:14: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:57:14: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:57:19: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:57:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:57:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:57:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.05_summits.bed INFO @ Tue, 16 Jun 2020 07:57:21: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (549 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:57:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:57:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:57:37: 1000000 INFO @ Tue, 16 Jun 2020 07:57:43: 2000000 INFO @ Tue, 16 Jun 2020 07:57:44: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:57:44: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:57:44: #1 total tags in treatment: 2191194 INFO @ Tue, 16 Jun 2020 07:57:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:57:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:57:44: #1 tags after filtering in treatment: 2191194 INFO @ Tue, 16 Jun 2020 07:57:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:57:44: #1 finished! INFO @ Tue, 16 Jun 2020 07:57:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:57:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:57:44: #2 number of paired peaks: 492 WARNING @ Tue, 16 Jun 2020 07:57:44: Fewer paired peaks (492) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 492 pairs to build model! INFO @ Tue, 16 Jun 2020 07:57:44: start model_add_line... INFO @ Tue, 16 Jun 2020 07:57:44: start X-correlation... INFO @ Tue, 16 Jun 2020 07:57:44: end of X-cor INFO @ Tue, 16 Jun 2020 07:57:44: #2 finished! INFO @ Tue, 16 Jun 2020 07:57:44: #2 predicted fragment length is 86 bps INFO @ Tue, 16 Jun 2020 07:57:44: #2 alternative fragment length(s) may be 39,86 bps INFO @ Tue, 16 Jun 2020 07:57:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.10_model.r INFO @ Tue, 16 Jun 2020 07:57:44: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:57:44: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:57:49: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:57:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:57:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:57:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.10_summits.bed INFO @ Tue, 16 Jun 2020 07:57:51: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (242 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:58:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:58:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:58:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:58:08: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:58:13: 2000000 INFO @ Tue, 16 Jun 2020 07:58:14: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:58:14: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:58:14: #1 total tags in treatment: 2191194 INFO @ Tue, 16 Jun 2020 07:58:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:58:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:58:14: #1 tags after filtering in treatment: 2191194 INFO @ Tue, 16 Jun 2020 07:58:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:58:14: #1 finished! INFO @ Tue, 16 Jun 2020 07:58:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:58:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:58:14: #2 number of paired peaks: 492 WARNING @ Tue, 16 Jun 2020 07:58:14: Fewer paired peaks (492) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 492 pairs to build model! INFO @ Tue, 16 Jun 2020 07:58:14: start model_add_line... INFO @ Tue, 16 Jun 2020 07:58:14: start X-correlation... INFO @ Tue, 16 Jun 2020 07:58:14: end of X-cor INFO @ Tue, 16 Jun 2020 07:58:14: #2 finished! INFO @ Tue, 16 Jun 2020 07:58:14: #2 predicted fragment length is 86 bps INFO @ Tue, 16 Jun 2020 07:58:14: #2 alternative fragment length(s) may be 39,86 bps INFO @ Tue, 16 Jun 2020 07:58:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.20_model.r INFO @ Tue, 16 Jun 2020 07:58:14: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:58:14: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:58:19: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:58:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:58:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:58:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147630/SRX147630.20_summits.bed INFO @ Tue, 16 Jun 2020 07:58:22: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (94 records, 4 fields): 1 millis CompletedMACS2peakCalling