Job ID = 6366472 SRX = SRX147625 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:53:56 prefetch.2.10.7: 1) Downloading 'SRR496302'... 2020-06-15T22:53:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:54:30 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:54:30 prefetch.2.10.7: 'SRR496302' is valid 2020-06-15T22:54:30 prefetch.2.10.7: 1) 'SRR496302' was downloaded successfully Read 3323286 spots for SRR496302/SRR496302.sra Written 3323286 spots for SRR496302/SRR496302.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:19 3323286 reads; of these: 3323286 (100.00%) were unpaired; of these: 1929396 (58.06%) aligned 0 times 1184441 (35.64%) aligned exactly 1 time 209449 (6.30%) aligned >1 times 41.94% overall alignment rate Time searching: 00:00:19 Overall time: 00:00:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 206335 / 1393890 = 0.1480 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:55:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:55:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:55:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:55:52: 1000000 INFO @ Tue, 16 Jun 2020 07:55:53: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:55:53: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:55:53: #1 total tags in treatment: 1187555 INFO @ Tue, 16 Jun 2020 07:55:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:55:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:55:53: #1 tags after filtering in treatment: 1187555 INFO @ Tue, 16 Jun 2020 07:55:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:55:53: #1 finished! INFO @ Tue, 16 Jun 2020 07:55:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:55:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:55:53: #2 number of paired peaks: 1081 INFO @ Tue, 16 Jun 2020 07:55:53: start model_add_line... INFO @ Tue, 16 Jun 2020 07:55:53: start X-correlation... INFO @ Tue, 16 Jun 2020 07:55:53: end of X-cor INFO @ Tue, 16 Jun 2020 07:55:53: #2 finished! INFO @ Tue, 16 Jun 2020 07:55:53: #2 predicted fragment length is 150 bps INFO @ Tue, 16 Jun 2020 07:55:53: #2 alternative fragment length(s) may be 150 bps INFO @ Tue, 16 Jun 2020 07:55:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.05_model.r INFO @ Tue, 16 Jun 2020 07:55:53: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:55:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:55:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:55:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:55:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:55:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.05_summits.bed INFO @ Tue, 16 Jun 2020 07:55:57: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1173 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:56:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:56:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:56:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:56:23: 1000000 INFO @ Tue, 16 Jun 2020 07:56:24: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:56:24: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:56:24: #1 total tags in treatment: 1187555 INFO @ Tue, 16 Jun 2020 07:56:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:56:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:56:24: #1 tags after filtering in treatment: 1187555 INFO @ Tue, 16 Jun 2020 07:56:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:56:24: #1 finished! INFO @ Tue, 16 Jun 2020 07:56:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:56:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:56:25: #2 number of paired peaks: 1081 INFO @ Tue, 16 Jun 2020 07:56:25: start model_add_line... INFO @ Tue, 16 Jun 2020 07:56:25: start X-correlation... INFO @ Tue, 16 Jun 2020 07:56:25: end of X-cor INFO @ Tue, 16 Jun 2020 07:56:25: #2 finished! INFO @ Tue, 16 Jun 2020 07:56:25: #2 predicted fragment length is 150 bps INFO @ Tue, 16 Jun 2020 07:56:25: #2 alternative fragment length(s) may be 150 bps INFO @ Tue, 16 Jun 2020 07:56:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.10_model.r INFO @ Tue, 16 Jun 2020 07:56:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:56:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:56:28: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:56:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:56:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:56:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.10_summits.bed INFO @ Tue, 16 Jun 2020 07:56:29: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (554 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:56:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:56:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:56:46: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:56:52: 1000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:56:53: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:56:53: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:56:53: #1 total tags in treatment: 1187555 INFO @ Tue, 16 Jun 2020 07:56:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:56:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:56:53: #1 tags after filtering in treatment: 1187555 INFO @ Tue, 16 Jun 2020 07:56:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:56:53: #1 finished! INFO @ Tue, 16 Jun 2020 07:56:53: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:56:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:56:53: #2 number of paired peaks: 1081 INFO @ Tue, 16 Jun 2020 07:56:53: start model_add_line... INFO @ Tue, 16 Jun 2020 07:56:53: start X-correlation... INFO @ Tue, 16 Jun 2020 07:56:53: end of X-cor INFO @ Tue, 16 Jun 2020 07:56:53: #2 finished! INFO @ Tue, 16 Jun 2020 07:56:53: #2 predicted fragment length is 150 bps INFO @ Tue, 16 Jun 2020 07:56:53: #2 alternative fragment length(s) may be 150 bps INFO @ Tue, 16 Jun 2020 07:56:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.20_model.r INFO @ Tue, 16 Jun 2020 07:56:53: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:56:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:56:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:56:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:56:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:56:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX147625/SRX147625.20_summits.bed INFO @ Tue, 16 Jun 2020 07:56:58: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (189 records, 4 fields): 1 millis CompletedMACS2peakCalling