Job ID = 6366452 SRX = SRX146516 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:46:56 prefetch.2.10.7: 1) Downloading 'SRR494684'... 2020-06-15T22:46:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:47:23 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:47:24 prefetch.2.10.7: 'SRR494684' is valid 2020-06-15T22:47:24 prefetch.2.10.7: 1) 'SRR494684' was downloaded successfully Read 4080576 spots for SRR494684/SRR494684.sra Written 4080576 spots for SRR494684/SRR494684.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:26 4080576 reads; of these: 4080576 (100.00%) were unpaired; of these: 1755573 (43.02%) aligned 0 times 1975201 (48.40%) aligned exactly 1 time 349802 (8.57%) aligned >1 times 56.98% overall alignment rate Time searching: 00:00:26 Overall time: 00:00:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 157769 / 2325003 = 0.0679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:49:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:49:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:49:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:49:06: 1000000 INFO @ Tue, 16 Jun 2020 07:49:11: 2000000 INFO @ Tue, 16 Jun 2020 07:49:11: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:49:11: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:49:11: #1 total tags in treatment: 2167234 INFO @ Tue, 16 Jun 2020 07:49:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:49:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:49:12: #1 tags after filtering in treatment: 2167234 INFO @ Tue, 16 Jun 2020 07:49:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:49:12: #1 finished! INFO @ Tue, 16 Jun 2020 07:49:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:49:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:49:12: #2 number of paired peaks: 1170 INFO @ Tue, 16 Jun 2020 07:49:12: start model_add_line... INFO @ Tue, 16 Jun 2020 07:49:12: start X-correlation... INFO @ Tue, 16 Jun 2020 07:49:12: end of X-cor INFO @ Tue, 16 Jun 2020 07:49:12: #2 finished! INFO @ Tue, 16 Jun 2020 07:49:12: #2 predicted fragment length is 115 bps INFO @ Tue, 16 Jun 2020 07:49:12: #2 alternative fragment length(s) may be 115 bps INFO @ Tue, 16 Jun 2020 07:49:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.05_model.r INFO @ Tue, 16 Jun 2020 07:49:12: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:49:12: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:49:17: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:49:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:49:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:49:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.05_summits.bed INFO @ Tue, 16 Jun 2020 07:49:20: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2229 records, 4 fields): 9 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:49:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:49:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:49:32: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:49:36: 1000000 INFO @ Tue, 16 Jun 2020 07:49:41: 2000000 INFO @ Tue, 16 Jun 2020 07:49:41: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:49:41: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:49:41: #1 total tags in treatment: 2167234 INFO @ Tue, 16 Jun 2020 07:49:41: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:49:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:49:42: #1 tags after filtering in treatment: 2167234 INFO @ Tue, 16 Jun 2020 07:49:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:49:42: #1 finished! INFO @ Tue, 16 Jun 2020 07:49:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:49:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:49:42: #2 number of paired peaks: 1170 INFO @ Tue, 16 Jun 2020 07:49:42: start model_add_line... INFO @ Tue, 16 Jun 2020 07:49:42: start X-correlation... INFO @ Tue, 16 Jun 2020 07:49:42: end of X-cor INFO @ Tue, 16 Jun 2020 07:49:42: #2 finished! INFO @ Tue, 16 Jun 2020 07:49:42: #2 predicted fragment length is 115 bps INFO @ Tue, 16 Jun 2020 07:49:42: #2 alternative fragment length(s) may be 115 bps INFO @ Tue, 16 Jun 2020 07:49:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.10_model.r INFO @ Tue, 16 Jun 2020 07:49:42: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:49:42: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:49:47: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:49:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:49:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:49:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.10_summits.bed INFO @ Tue, 16 Jun 2020 07:49:50: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (1289 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:50:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:50:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:50:02: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:50:06: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:50:11: 2000000 INFO @ Tue, 16 Jun 2020 07:50:12: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:50:12: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:50:12: #1 total tags in treatment: 2167234 INFO @ Tue, 16 Jun 2020 07:50:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:50:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:50:12: #1 tags after filtering in treatment: 2167234 INFO @ Tue, 16 Jun 2020 07:50:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:50:12: #1 finished! INFO @ Tue, 16 Jun 2020 07:50:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:50:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:50:12: #2 number of paired peaks: 1170 INFO @ Tue, 16 Jun 2020 07:50:12: start model_add_line... INFO @ Tue, 16 Jun 2020 07:50:12: start X-correlation... INFO @ Tue, 16 Jun 2020 07:50:12: end of X-cor INFO @ Tue, 16 Jun 2020 07:50:12: #2 finished! INFO @ Tue, 16 Jun 2020 07:50:12: #2 predicted fragment length is 115 bps INFO @ Tue, 16 Jun 2020 07:50:12: #2 alternative fragment length(s) may be 115 bps INFO @ Tue, 16 Jun 2020 07:50:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.20_model.r INFO @ Tue, 16 Jun 2020 07:50:12: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:50:12: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:50:17: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:50:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:50:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:50:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX146516/SRX146516.20_summits.bed INFO @ Tue, 16 Jun 2020 07:50:20: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (748 records, 4 fields): 2 millis CompletedMACS2peakCalling