Job ID = 6366393 SRX = SRX1388767 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:42:07 prefetch.2.10.7: 1) Downloading 'SRR2832483'... 2020-06-15T22:42:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:42:39 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:42:39 prefetch.2.10.7: 'SRR2832483' is valid 2020-06-15T22:42:39 prefetch.2.10.7: 1) 'SRR2832483' was downloaded successfully Read 5332698 spots for SRR2832483/SRR2832483.sra Written 5332698 spots for SRR2832483/SRR2832483.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:07 5332698 reads; of these: 5332698 (100.00%) were unpaired; of these: 430756 (8.08%) aligned 0 times 4248876 (79.68%) aligned exactly 1 time 653066 (12.25%) aligned >1 times 91.92% overall alignment rate Time searching: 00:01:07 Overall time: 00:01:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 482628 / 4901942 = 0.0985 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:45:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:45:37: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:45:37: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:45:43: 1000000 INFO @ Tue, 16 Jun 2020 07:45:48: 2000000 INFO @ Tue, 16 Jun 2020 07:45:53: 3000000 INFO @ Tue, 16 Jun 2020 07:45:57: 4000000 INFO @ Tue, 16 Jun 2020 07:46:00: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:46:00: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:46:00: #1 total tags in treatment: 4419314 INFO @ Tue, 16 Jun 2020 07:46:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:46:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:46:00: #1 tags after filtering in treatment: 4419314 INFO @ Tue, 16 Jun 2020 07:46:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:46:00: #1 finished! INFO @ Tue, 16 Jun 2020 07:46:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:46:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:46:00: #2 number of paired peaks: 2771 INFO @ Tue, 16 Jun 2020 07:46:00: start model_add_line... INFO @ Tue, 16 Jun 2020 07:46:00: start X-correlation... INFO @ Tue, 16 Jun 2020 07:46:00: end of X-cor INFO @ Tue, 16 Jun 2020 07:46:00: #2 finished! INFO @ Tue, 16 Jun 2020 07:46:00: #2 predicted fragment length is 175 bps INFO @ Tue, 16 Jun 2020 07:46:00: #2 alternative fragment length(s) may be 175 bps INFO @ Tue, 16 Jun 2020 07:46:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.05_model.r INFO @ Tue, 16 Jun 2020 07:46:00: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:46:00: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:46:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:46:07: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:46:07: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:46:12: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:46:13: 1000000 INFO @ Tue, 16 Jun 2020 07:46:17: 2000000 INFO @ Tue, 16 Jun 2020 07:46:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:46:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:46:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.05_summits.bed INFO @ Tue, 16 Jun 2020 07:46:19: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3909 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:46:22: 3000000 INFO @ Tue, 16 Jun 2020 07:46:27: 4000000 INFO @ Tue, 16 Jun 2020 07:46:30: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:46:30: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:46:30: #1 total tags in treatment: 4419314 INFO @ Tue, 16 Jun 2020 07:46:30: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:46:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:46:30: #1 tags after filtering in treatment: 4419314 INFO @ Tue, 16 Jun 2020 07:46:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:46:30: #1 finished! INFO @ Tue, 16 Jun 2020 07:46:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:46:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:46:30: #2 number of paired peaks: 2771 INFO @ Tue, 16 Jun 2020 07:46:30: start model_add_line... INFO @ Tue, 16 Jun 2020 07:46:30: start X-correlation... INFO @ Tue, 16 Jun 2020 07:46:30: end of X-cor INFO @ Tue, 16 Jun 2020 07:46:30: #2 finished! INFO @ Tue, 16 Jun 2020 07:46:30: #2 predicted fragment length is 175 bps INFO @ Tue, 16 Jun 2020 07:46:30: #2 alternative fragment length(s) may be 175 bps INFO @ Tue, 16 Jun 2020 07:46:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.10_model.r INFO @ Tue, 16 Jun 2020 07:46:30: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:46:30: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:46:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:46:37: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:46:37: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:46:43: 1000000 INFO @ Tue, 16 Jun 2020 07:46:43: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:46:48: 2000000 INFO @ Tue, 16 Jun 2020 07:46:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:46:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:46:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.10_summits.bed INFO @ Tue, 16 Jun 2020 07:46:50: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (2047 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:46:53: 3000000 INFO @ Tue, 16 Jun 2020 07:46:59: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:47:01: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 07:47:01: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 07:47:01: #1 total tags in treatment: 4419314 INFO @ Tue, 16 Jun 2020 07:47:01: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:47:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:47:01: #1 tags after filtering in treatment: 4419314 INFO @ Tue, 16 Jun 2020 07:47:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:47:01: #1 finished! INFO @ Tue, 16 Jun 2020 07:47:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:47:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:47:02: #2 number of paired peaks: 2771 INFO @ Tue, 16 Jun 2020 07:47:02: start model_add_line... INFO @ Tue, 16 Jun 2020 07:47:02: start X-correlation... INFO @ Tue, 16 Jun 2020 07:47:02: end of X-cor INFO @ Tue, 16 Jun 2020 07:47:02: #2 finished! INFO @ Tue, 16 Jun 2020 07:47:02: #2 predicted fragment length is 175 bps INFO @ Tue, 16 Jun 2020 07:47:02: #2 alternative fragment length(s) may be 175 bps INFO @ Tue, 16 Jun 2020 07:47:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.20_model.r INFO @ Tue, 16 Jun 2020 07:47:02: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:47:02: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:47:15: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:47:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:47:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:47:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX1388767/SRX1388767.20_summits.bed INFO @ Tue, 16 Jun 2020 07:47:22: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (753 records, 4 fields): 3 millis CompletedMACS2peakCalling