Job ID = 16436260 SRX = SRX13110391 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:28 37731320 reads; of these: 37731320 (100.00%) were unpaired; of these: 2529453 (6.70%) aligned 0 times 29714521 (78.75%) aligned exactly 1 time 5487346 (14.54%) aligned >1 times 93.30% overall alignment rate Time searching: 00:13:28 Overall time: 00:13:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5476643 / 35201867 = 0.1556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:39:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:39:31: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:39:31: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:39:38: 1000000 INFO @ Tue, 02 Aug 2022 11:39:45: 2000000 INFO @ Tue, 02 Aug 2022 11:39:51: 3000000 INFO @ Tue, 02 Aug 2022 11:39:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:40:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:40:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:40:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:40:03: 5000000 INFO @ Tue, 02 Aug 2022 11:40:10: 1000000 INFO @ Tue, 02 Aug 2022 11:40:11: 6000000 INFO @ Tue, 02 Aug 2022 11:40:18: 7000000 INFO @ Tue, 02 Aug 2022 11:40:19: 2000000 INFO @ Tue, 02 Aug 2022 11:40:26: 8000000 INFO @ Tue, 02 Aug 2022 11:40:28: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:40:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:40:31: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:40:31: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:40:33: 9000000 INFO @ Tue, 02 Aug 2022 11:40:36: 4000000 INFO @ Tue, 02 Aug 2022 11:40:39: 1000000 INFO @ Tue, 02 Aug 2022 11:40:41: 10000000 INFO @ Tue, 02 Aug 2022 11:40:43: 5000000 INFO @ Tue, 02 Aug 2022 11:40:46: 2000000 INFO @ Tue, 02 Aug 2022 11:40:48: 11000000 INFO @ Tue, 02 Aug 2022 11:40:51: 6000000 INFO @ Tue, 02 Aug 2022 11:40:54: 3000000 INFO @ Tue, 02 Aug 2022 11:40:56: 12000000 INFO @ Tue, 02 Aug 2022 11:40:58: 7000000 INFO @ Tue, 02 Aug 2022 11:41:01: 4000000 INFO @ Tue, 02 Aug 2022 11:41:03: 13000000 INFO @ Tue, 02 Aug 2022 11:41:06: 8000000 INFO @ Tue, 02 Aug 2022 11:41:09: 5000000 INFO @ Tue, 02 Aug 2022 11:41:10: 14000000 INFO @ Tue, 02 Aug 2022 11:41:13: 9000000 INFO @ Tue, 02 Aug 2022 11:41:16: 6000000 INFO @ Tue, 02 Aug 2022 11:41:17: 15000000 INFO @ Tue, 02 Aug 2022 11:41:20: 10000000 INFO @ Tue, 02 Aug 2022 11:41:23: 7000000 INFO @ Tue, 02 Aug 2022 11:41:25: 16000000 INFO @ Tue, 02 Aug 2022 11:41:28: 11000000 INFO @ Tue, 02 Aug 2022 11:41:30: 8000000 INFO @ Tue, 02 Aug 2022 11:41:32: 17000000 INFO @ Tue, 02 Aug 2022 11:41:35: 12000000 INFO @ Tue, 02 Aug 2022 11:41:38: 9000000 INFO @ Tue, 02 Aug 2022 11:41:39: 18000000 INFO @ Tue, 02 Aug 2022 11:41:42: 13000000 INFO @ Tue, 02 Aug 2022 11:41:45: 10000000 INFO @ Tue, 02 Aug 2022 11:41:46: 19000000 INFO @ Tue, 02 Aug 2022 11:41:50: 14000000 INFO @ Tue, 02 Aug 2022 11:41:53: 11000000 INFO @ Tue, 02 Aug 2022 11:41:53: 20000000 INFO @ Tue, 02 Aug 2022 11:41:57: 15000000 INFO @ Tue, 02 Aug 2022 11:42:00: 12000000 INFO @ Tue, 02 Aug 2022 11:42:01: 21000000 INFO @ Tue, 02 Aug 2022 11:42:04: 16000000 INFO @ Tue, 02 Aug 2022 11:42:07: 13000000 INFO @ Tue, 02 Aug 2022 11:42:08: 22000000 INFO @ Tue, 02 Aug 2022 11:42:11: 17000000 INFO @ Tue, 02 Aug 2022 11:42:14: 14000000 INFO @ Tue, 02 Aug 2022 11:42:15: 23000000 INFO @ Tue, 02 Aug 2022 11:42:18: 18000000 INFO @ Tue, 02 Aug 2022 11:42:22: 15000000 INFO @ Tue, 02 Aug 2022 11:42:22: 24000000 INFO @ Tue, 02 Aug 2022 11:42:26: 19000000 INFO @ Tue, 02 Aug 2022 11:42:29: 16000000 INFO @ Tue, 02 Aug 2022 11:42:30: 25000000 INFO @ Tue, 02 Aug 2022 11:42:33: 20000000 INFO @ Tue, 02 Aug 2022 11:42:36: 17000000 INFO @ Tue, 02 Aug 2022 11:42:37: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 11:42:40: 21000000 INFO @ Tue, 02 Aug 2022 11:42:43: 18000000 INFO @ Tue, 02 Aug 2022 11:42:44: 27000000 INFO @ Tue, 02 Aug 2022 11:42:47: 22000000 INFO @ Tue, 02 Aug 2022 11:42:50: 19000000 INFO @ Tue, 02 Aug 2022 11:42:51: 28000000 INFO @ Tue, 02 Aug 2022 11:42:55: 23000000 INFO @ Tue, 02 Aug 2022 11:42:58: 20000000 INFO @ Tue, 02 Aug 2022 11:42:58: 29000000 INFO @ Tue, 02 Aug 2022 11:43:02: 24000000 INFO @ Tue, 02 Aug 2022 11:43:03: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 11:43:03: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 11:43:03: #1 total tags in treatment: 29725224 INFO @ Tue, 02 Aug 2022 11:43:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:43:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:43:04: #1 tags after filtering in treatment: 29725224 INFO @ Tue, 02 Aug 2022 11:43:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:43:04: #1 finished! INFO @ Tue, 02 Aug 2022 11:43:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:43:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:43:05: 21000000 INFO @ Tue, 02 Aug 2022 11:43:05: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 11:43:05: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:43:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:43:09: 25000000 INFO @ Tue, 02 Aug 2022 11:43:12: 22000000 INFO @ Tue, 02 Aug 2022 11:43:16: 26000000 INFO @ Tue, 02 Aug 2022 11:43:19: 23000000 INFO @ Tue, 02 Aug 2022 11:43:23: 27000000 INFO @ Tue, 02 Aug 2022 11:43:26: 24000000 INFO @ Tue, 02 Aug 2022 11:43:30: 28000000 INFO @ Tue, 02 Aug 2022 11:43:33: 25000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 11:43:37: 29000000 INFO @ Tue, 02 Aug 2022 11:43:40: 26000000 INFO @ Tue, 02 Aug 2022 11:43:42: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 11:43:42: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 11:43:42: #1 total tags in treatment: 29725224 INFO @ Tue, 02 Aug 2022 11:43:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:43:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:43:43: #1 tags after filtering in treatment: 29725224 INFO @ Tue, 02 Aug 2022 11:43:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:43:43: #1 finished! INFO @ Tue, 02 Aug 2022 11:43:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:43:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:43:45: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 11:43:45: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:43:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:43:47: 27000000 INFO @ Tue, 02 Aug 2022 11:43:53: 28000000 INFO @ Tue, 02 Aug 2022 11:43:59: 29000000 INFO @ Tue, 02 Aug 2022 11:44:04: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 11:44:04: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 11:44:04: #1 total tags in treatment: 29725224 INFO @ Tue, 02 Aug 2022 11:44:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:44:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:44:04: #1 tags after filtering in treatment: 29725224 INFO @ Tue, 02 Aug 2022 11:44:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:44:04: #1 finished! INFO @ Tue, 02 Aug 2022 11:44:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:44:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:44:06: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 11:44:06: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:44:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX13110391/SRX13110391.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling