Job ID = 16436815 SRX = SRX12572573 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 02:04:54 47031001 reads; of these: 47031001 (100.00%) were paired; of these: 4903491 (10.43%) aligned concordantly 0 times 36427465 (77.45%) aligned concordantly exactly 1 time 5700045 (12.12%) aligned concordantly >1 times ---- 4903491 pairs aligned concordantly 0 times; of these: 1719020 (35.06%) aligned discordantly 1 time ---- 3184471 pairs aligned 0 times concordantly or discordantly; of these: 6368942 mates make up the pairs; of these: 4886361 (76.72%) aligned 0 times 897061 (14.08%) aligned exactly 1 time 585520 (9.19%) aligned >1 times 94.81% overall alignment rate Time searching: 02:04:55 Overall time: 02:04:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 68 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 9896593 / 43742460 = 0.2262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:38:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:38:15: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:38:15: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:38:25: 1000000 INFO @ Tue, 02 Aug 2022 14:38:35: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:38:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:38:44: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:38:44: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:38:45: 3000000 INFO @ Tue, 02 Aug 2022 14:38:55: 4000000 INFO @ Tue, 02 Aug 2022 14:38:58: 1000000 INFO @ Tue, 02 Aug 2022 14:39:06: 5000000 INFO @ Tue, 02 Aug 2022 14:39:11: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:39:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:39:14: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:39:14: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:39:16: 6000000 INFO @ Tue, 02 Aug 2022 14:39:24: 3000000 INFO @ Tue, 02 Aug 2022 14:39:27: 1000000 INFO @ Tue, 02 Aug 2022 14:39:27: 7000000 INFO @ Tue, 02 Aug 2022 14:39:38: 4000000 INFO @ Tue, 02 Aug 2022 14:39:38: 8000000 INFO @ Tue, 02 Aug 2022 14:39:39: 2000000 INFO @ Tue, 02 Aug 2022 14:39:49: 9000000 INFO @ Tue, 02 Aug 2022 14:39:51: 5000000 INFO @ Tue, 02 Aug 2022 14:39:51: 3000000 INFO @ Tue, 02 Aug 2022 14:40:00: 10000000 INFO @ Tue, 02 Aug 2022 14:40:04: 4000000 INFO @ Tue, 02 Aug 2022 14:40:04: 6000000 INFO @ Tue, 02 Aug 2022 14:40:11: 11000000 INFO @ Tue, 02 Aug 2022 14:40:16: 5000000 INFO @ Tue, 02 Aug 2022 14:40:18: 7000000 INFO @ Tue, 02 Aug 2022 14:40:22: 12000000 INFO @ Tue, 02 Aug 2022 14:40:27: 6000000 INFO @ Tue, 02 Aug 2022 14:40:32: 8000000 INFO @ Tue, 02 Aug 2022 14:40:33: 13000000 INFO @ Tue, 02 Aug 2022 14:40:39: 7000000 INFO @ Tue, 02 Aug 2022 14:40:44: 14000000 INFO @ Tue, 02 Aug 2022 14:40:46: 9000000 INFO @ Tue, 02 Aug 2022 14:40:51: 8000000 INFO @ Tue, 02 Aug 2022 14:40:55: 15000000 INFO @ Tue, 02 Aug 2022 14:41:01: 10000000 INFO @ Tue, 02 Aug 2022 14:41:03: 9000000 INFO @ Tue, 02 Aug 2022 14:41:06: 16000000 INFO @ Tue, 02 Aug 2022 14:41:16: 10000000 INFO @ Tue, 02 Aug 2022 14:41:16: 11000000 INFO @ Tue, 02 Aug 2022 14:41:18: 17000000 INFO @ Tue, 02 Aug 2022 14:41:29: 11000000 INFO @ Tue, 02 Aug 2022 14:41:29: 18000000 INFO @ Tue, 02 Aug 2022 14:41:30: 12000000 INFO @ Tue, 02 Aug 2022 14:41:42: 19000000 INFO @ Tue, 02 Aug 2022 14:41:42: 12000000 INFO @ Tue, 02 Aug 2022 14:41:45: 13000000 INFO @ Tue, 02 Aug 2022 14:41:54: 20000000 INFO @ Tue, 02 Aug 2022 14:41:56: 13000000 INFO @ Tue, 02 Aug 2022 14:42:00: 14000000 INFO @ Tue, 02 Aug 2022 14:42:06: 21000000 INFO @ Tue, 02 Aug 2022 14:42:09: 14000000 INFO @ Tue, 02 Aug 2022 14:42:15: 15000000 INFO @ Tue, 02 Aug 2022 14:42:18: 22000000 INFO @ Tue, 02 Aug 2022 14:42:22: 15000000 INFO @ Tue, 02 Aug 2022 14:42:30: 16000000 INFO @ Tue, 02 Aug 2022 14:42:30: 23000000 INFO @ Tue, 02 Aug 2022 14:42:34: 16000000 INFO @ Tue, 02 Aug 2022 14:42:41: 17000000 INFO @ Tue, 02 Aug 2022 14:42:42: 24000000 INFO @ Tue, 02 Aug 2022 14:42:44: 17000000 INFO @ Tue, 02 Aug 2022 14:42:53: 18000000 INFO @ Tue, 02 Aug 2022 14:42:54: 25000000 INFO @ Tue, 02 Aug 2022 14:42:55: 18000000 INFO @ Tue, 02 Aug 2022 14:43:05: 19000000 INFO @ Tue, 02 Aug 2022 14:43:05: 26000000 INFO @ Tue, 02 Aug 2022 14:43:06: 19000000 INFO @ Tue, 02 Aug 2022 14:43:15: 20000000 INFO @ Tue, 02 Aug 2022 14:43:16: 20000000 INFO @ Tue, 02 Aug 2022 14:43:17: 27000000 INFO @ Tue, 02 Aug 2022 14:43:26: 21000000 INFO @ Tue, 02 Aug 2022 14:43:27: 21000000 INFO @ Tue, 02 Aug 2022 14:43:28: 28000000 INFO @ Tue, 02 Aug 2022 14:43:37: 22000000 INFO @ Tue, 02 Aug 2022 14:43:38: 22000000 INFO @ Tue, 02 Aug 2022 14:43:40: 29000000 INFO @ Tue, 02 Aug 2022 14:43:48: 23000000 INFO @ Tue, 02 Aug 2022 14:43:49: 23000000 INFO @ Tue, 02 Aug 2022 14:43:52: 30000000 INFO @ Tue, 02 Aug 2022 14:43:59: 24000000 INFO @ Tue, 02 Aug 2022 14:43:59: 24000000 INFO @ Tue, 02 Aug 2022 14:44:03: 31000000 INFO @ Tue, 02 Aug 2022 14:44:10: 25000000 INFO @ Tue, 02 Aug 2022 14:44:10: 25000000 INFO @ Tue, 02 Aug 2022 14:44:15: 32000000 INFO @ Tue, 02 Aug 2022 14:44:20: 26000000 INFO @ Tue, 02 Aug 2022 14:44:21: 26000000 INFO @ Tue, 02 Aug 2022 14:44:26: 33000000 INFO @ Tue, 02 Aug 2022 14:44:31: 27000000 INFO @ Tue, 02 Aug 2022 14:44:32: 27000000 INFO @ Tue, 02 Aug 2022 14:44:38: 34000000 INFO @ Tue, 02 Aug 2022 14:44:42: 28000000 INFO @ Tue, 02 Aug 2022 14:44:44: 28000000 INFO @ Tue, 02 Aug 2022 14:44:49: 35000000 INFO @ Tue, 02 Aug 2022 14:44:53: 29000000 INFO @ Tue, 02 Aug 2022 14:44:55: 29000000 INFO @ Tue, 02 Aug 2022 14:45:00: 36000000 INFO @ Tue, 02 Aug 2022 14:45:05: 30000000 INFO @ Tue, 02 Aug 2022 14:45:08: 30000000 INFO @ Tue, 02 Aug 2022 14:45:12: 37000000 INFO @ Tue, 02 Aug 2022 14:45:15: 31000000 INFO @ Tue, 02 Aug 2022 14:45:20: 31000000 INFO @ Tue, 02 Aug 2022 14:45:23: 38000000 INFO @ Tue, 02 Aug 2022 14:45:26: 32000000 INFO @ Tue, 02 Aug 2022 14:45:31: 32000000 INFO @ Tue, 02 Aug 2022 14:45:35: 39000000 INFO @ Tue, 02 Aug 2022 14:45:37: 33000000 INFO @ Tue, 02 Aug 2022 14:45:42: 33000000 INFO @ Tue, 02 Aug 2022 14:45:46: 40000000 INFO @ Tue, 02 Aug 2022 14:45:49: 34000000 INFO @ Tue, 02 Aug 2022 14:45:54: 34000000 INFO @ Tue, 02 Aug 2022 14:45:58: 41000000 INFO @ Tue, 02 Aug 2022 14:46:00: 35000000 INFO @ Tue, 02 Aug 2022 14:46:06: 35000000 INFO @ Tue, 02 Aug 2022 14:46:09: 42000000 INFO @ Tue, 02 Aug 2022 14:46:12: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:46:18: 36000000 INFO @ Tue, 02 Aug 2022 14:46:21: 43000000 INFO @ Tue, 02 Aug 2022 14:46:23: 37000000 INFO @ Tue, 02 Aug 2022 14:46:30: 37000000 INFO @ Tue, 02 Aug 2022 14:46:33: 44000000 INFO @ Tue, 02 Aug 2022 14:46:34: 38000000 INFO @ Tue, 02 Aug 2022 14:46:42: 38000000 INFO @ Tue, 02 Aug 2022 14:46:44: 39000000 INFO @ Tue, 02 Aug 2022 14:46:44: 45000000 INFO @ Tue, 02 Aug 2022 14:46:54: 39000000 INFO @ Tue, 02 Aug 2022 14:46:55: 40000000 INFO @ Tue, 02 Aug 2022 14:46:56: 46000000 INFO @ Tue, 02 Aug 2022 14:47:05: 41000000 INFO @ Tue, 02 Aug 2022 14:47:06: 40000000 INFO @ Tue, 02 Aug 2022 14:47:08: 47000000 INFO @ Tue, 02 Aug 2022 14:47:16: 42000000 INFO @ Tue, 02 Aug 2022 14:47:18: 41000000 INFO @ Tue, 02 Aug 2022 14:47:19: 48000000 INFO @ Tue, 02 Aug 2022 14:47:26: 43000000 INFO @ Tue, 02 Aug 2022 14:47:30: 49000000 INFO @ Tue, 02 Aug 2022 14:47:30: 42000000 INFO @ Tue, 02 Aug 2022 14:47:37: 44000000 INFO @ Tue, 02 Aug 2022 14:47:42: 50000000 INFO @ Tue, 02 Aug 2022 14:47:43: 43000000 INFO @ Tue, 02 Aug 2022 14:47:48: 45000000 INFO @ Tue, 02 Aug 2022 14:47:53: 51000000 INFO @ Tue, 02 Aug 2022 14:47:54: 44000000 INFO @ Tue, 02 Aug 2022 14:47:58: 46000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:48:05: 52000000 INFO @ Tue, 02 Aug 2022 14:48:07: 45000000 INFO @ Tue, 02 Aug 2022 14:48:09: 47000000 INFO @ Tue, 02 Aug 2022 14:48:16: 53000000 INFO @ Tue, 02 Aug 2022 14:48:19: 46000000 INFO @ Tue, 02 Aug 2022 14:48:20: 48000000 INFO @ Tue, 02 Aug 2022 14:48:28: 54000000 INFO @ Tue, 02 Aug 2022 14:48:31: 47000000 INFO @ Tue, 02 Aug 2022 14:48:31: 49000000 INFO @ Tue, 02 Aug 2022 14:48:40: 55000000 INFO @ Tue, 02 Aug 2022 14:48:43: 50000000 INFO @ Tue, 02 Aug 2022 14:48:43: 48000000 INFO @ Tue, 02 Aug 2022 14:48:52: 56000000 INFO @ Tue, 02 Aug 2022 14:48:54: 51000000 INFO @ Tue, 02 Aug 2022 14:48:55: 49000000 INFO @ Tue, 02 Aug 2022 14:49:04: 57000000 INFO @ Tue, 02 Aug 2022 14:49:06: 52000000 INFO @ Tue, 02 Aug 2022 14:49:07: 50000000 INFO @ Tue, 02 Aug 2022 14:49:16: 58000000 INFO @ Tue, 02 Aug 2022 14:49:19: 53000000 INFO @ Tue, 02 Aug 2022 14:49:19: 51000000 INFO @ Tue, 02 Aug 2022 14:49:28: 59000000 INFO @ Tue, 02 Aug 2022 14:49:30: 52000000 INFO @ Tue, 02 Aug 2022 14:49:31: 54000000 INFO @ Tue, 02 Aug 2022 14:49:40: 60000000 INFO @ Tue, 02 Aug 2022 14:49:41: 53000000 INFO @ Tue, 02 Aug 2022 14:49:43: 55000000 INFO @ Tue, 02 Aug 2022 14:49:52: 54000000 INFO @ Tue, 02 Aug 2022 14:49:52: 61000000 INFO @ Tue, 02 Aug 2022 14:49:55: 56000000 INFO @ Tue, 02 Aug 2022 14:50:03: 55000000 INFO @ Tue, 02 Aug 2022 14:50:06: 62000000 INFO @ Tue, 02 Aug 2022 14:50:06: 57000000 INFO @ Tue, 02 Aug 2022 14:50:14: 56000000 INFO @ Tue, 02 Aug 2022 14:50:18: 63000000 INFO @ Tue, 02 Aug 2022 14:50:18: 58000000 INFO @ Tue, 02 Aug 2022 14:50:25: 57000000 INFO @ Tue, 02 Aug 2022 14:50:29: 59000000 INFO @ Tue, 02 Aug 2022 14:50:30: 64000000 INFO @ Tue, 02 Aug 2022 14:50:37: 58000000 INFO @ Tue, 02 Aug 2022 14:50:40: 60000000 INFO @ Tue, 02 Aug 2022 14:50:42: 65000000 INFO @ Tue, 02 Aug 2022 14:50:49: 59000000 INFO @ Tue, 02 Aug 2022 14:50:50: 61000000 INFO @ Tue, 02 Aug 2022 14:50:56: 66000000 INFO @ Tue, 02 Aug 2022 14:51:01: 62000000 INFO @ Tue, 02 Aug 2022 14:51:02: 60000000 INFO @ Tue, 02 Aug 2022 14:51:10: 67000000 INFO @ Tue, 02 Aug 2022 14:51:11: 63000000 INFO @ Tue, 02 Aug 2022 14:51:15: 61000000 INFO @ Tue, 02 Aug 2022 14:51:22: 64000000 INFO @ Tue, 02 Aug 2022 14:51:23: 68000000 INFO @ Tue, 02 Aug 2022 14:51:29: 62000000 INFO @ Tue, 02 Aug 2022 14:51:32: 65000000 INFO @ Tue, 02 Aug 2022 14:51:37: 69000000 INFO @ Tue, 02 Aug 2022 14:51:42: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:51:42: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:51:42: #1 total tags in treatment: 32500302 INFO @ Tue, 02 Aug 2022 14:51:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:51:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:51:42: 63000000 INFO @ Tue, 02 Aug 2022 14:51:43: #1 tags after filtering in treatment: 27941097 INFO @ Tue, 02 Aug 2022 14:51:43: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 14:51:43: #1 finished! INFO @ Tue, 02 Aug 2022 14:51:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:51:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:51:43: 66000000 INFO @ Tue, 02 Aug 2022 14:51:45: #2 number of paired peaks: 115 WARNING @ Tue, 02 Aug 2022 14:51:45: Fewer paired peaks (115) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 115 pairs to build model! INFO @ Tue, 02 Aug 2022 14:51:45: start model_add_line... INFO @ Tue, 02 Aug 2022 14:51:45: start X-correlation... INFO @ Tue, 02 Aug 2022 14:51:45: end of X-cor INFO @ Tue, 02 Aug 2022 14:51:45: #2 finished! INFO @ Tue, 02 Aug 2022 14:51:45: #2 predicted fragment length is 150 bps INFO @ Tue, 02 Aug 2022 14:51:45: #2 alternative fragment length(s) may be 3,116,126,150 bps INFO @ Tue, 02 Aug 2022 14:51:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.05_model.r WARNING @ Tue, 02 Aug 2022 14:51:45: #2 Since the d (150) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:51:45: #2 You may need to consider one of the other alternative d(s): 3,116,126,150 WARNING @ Tue, 02 Aug 2022 14:51:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:51:45: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:51:45: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:51:54: 67000000 INFO @ Tue, 02 Aug 2022 14:51:55: 64000000 INFO @ Tue, 02 Aug 2022 14:52:04: 68000000 INFO @ Tue, 02 Aug 2022 14:52:08: 65000000 INFO @ Tue, 02 Aug 2022 14:52:15: 69000000 INFO @ Tue, 02 Aug 2022 14:52:19: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:52:19: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:52:19: #1 total tags in treatment: 32500302 INFO @ Tue, 02 Aug 2022 14:52:19: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:52:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:52:20: #1 tags after filtering in treatment: 27941097 INFO @ Tue, 02 Aug 2022 14:52:20: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 14:52:20: #1 finished! INFO @ Tue, 02 Aug 2022 14:52:20: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:52:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:52:21: 66000000 INFO @ Tue, 02 Aug 2022 14:52:21: #2 number of paired peaks: 115 WARNING @ Tue, 02 Aug 2022 14:52:21: Fewer paired peaks (115) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 115 pairs to build model! INFO @ Tue, 02 Aug 2022 14:52:21: start model_add_line... INFO @ Tue, 02 Aug 2022 14:52:22: start X-correlation... INFO @ Tue, 02 Aug 2022 14:52:22: end of X-cor INFO @ Tue, 02 Aug 2022 14:52:22: #2 finished! INFO @ Tue, 02 Aug 2022 14:52:22: #2 predicted fragment length is 150 bps INFO @ Tue, 02 Aug 2022 14:52:22: #2 alternative fragment length(s) may be 3,116,126,150 bps INFO @ Tue, 02 Aug 2022 14:52:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.20_model.r WARNING @ Tue, 02 Aug 2022 14:52:22: #2 Since the d (150) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:52:22: #2 You may need to consider one of the other alternative d(s): 3,116,126,150 WARNING @ Tue, 02 Aug 2022 14:52:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:52:22: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:52:22: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:52:32: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:52:34: 67000000 INFO @ Tue, 02 Aug 2022 14:52:48: 68000000 INFO @ Tue, 02 Aug 2022 14:52:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:52:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:52:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.05_summits.bed INFO @ Tue, 02 Aug 2022 14:52:55: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (3736 records, 4 fields): 44 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:53:02: 69000000 INFO @ Tue, 02 Aug 2022 14:53:08: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:53:08: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 14:53:08: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 14:53:08: #1 total tags in treatment: 32500302 INFO @ Tue, 02 Aug 2022 14:53:08: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:53:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:53:08: #1 tags after filtering in treatment: 27941097 INFO @ Tue, 02 Aug 2022 14:53:08: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 14:53:08: #1 finished! INFO @ Tue, 02 Aug 2022 14:53:08: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:53:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:53:10: #2 number of paired peaks: 115 WARNING @ Tue, 02 Aug 2022 14:53:10: Fewer paired peaks (115) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 115 pairs to build model! INFO @ Tue, 02 Aug 2022 14:53:10: start model_add_line... INFO @ Tue, 02 Aug 2022 14:53:10: start X-correlation... INFO @ Tue, 02 Aug 2022 14:53:10: end of X-cor INFO @ Tue, 02 Aug 2022 14:53:10: #2 finished! INFO @ Tue, 02 Aug 2022 14:53:10: #2 predicted fragment length is 150 bps INFO @ Tue, 02 Aug 2022 14:53:10: #2 alternative fragment length(s) may be 3,116,126,150 bps INFO @ Tue, 02 Aug 2022 14:53:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.10_model.r WARNING @ Tue, 02 Aug 2022 14:53:10: #2 Since the d (150) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 14:53:10: #2 You may need to consider one of the other alternative d(s): 3,116,126,150 WARNING @ Tue, 02 Aug 2022 14:53:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 14:53:10: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:53:10: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:53:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:53:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:53:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.20_summits.bed INFO @ Tue, 02 Aug 2022 14:53:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (927 records, 4 fields): 28 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:53:57: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:54:18: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:54:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:54:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX12572573/SRX12572573.10_summits.bed INFO @ Tue, 02 Aug 2022 14:54:18: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (2241 records, 4 fields): 26 millis CompletedMACS2peakCalling