Job ID = 16436572 SRX = SRX12572570 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:27:10 43663273 reads; of these: 43663273 (100.00%) were paired; of these: 4514041 (10.34%) aligned concordantly 0 times 33313526 (76.30%) aligned concordantly exactly 1 time 5835706 (13.37%) aligned concordantly >1 times ---- 4514041 pairs aligned concordantly 0 times; of these: 1792436 (39.71%) aligned discordantly 1 time ---- 2721605 pairs aligned 0 times concordantly or discordantly; of these: 5443210 mates make up the pairs; of these: 4236362 (77.83%) aligned 0 times 650114 (11.94%) aligned exactly 1 time 556734 (10.23%) aligned >1 times 95.15% overall alignment rate Time searching: 01:27:10 Overall time: 01:27:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 64 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 6831084 / 40804403 = 0.1674 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:39:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:39:21: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:39:21: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:39:29: 1000000 INFO @ Tue, 02 Aug 2022 13:39:38: 2000000 INFO @ Tue, 02 Aug 2022 13:39:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:39:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:39:50: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:39:50: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:39:55: 4000000 INFO @ Tue, 02 Aug 2022 13:39:59: 1000000 INFO @ Tue, 02 Aug 2022 13:40:04: 5000000 INFO @ Tue, 02 Aug 2022 13:40:09: 2000000 INFO @ Tue, 02 Aug 2022 13:40:14: 6000000 INFO @ Tue, 02 Aug 2022 13:40:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:40:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:40:20: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:40:20: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:40:23: 7000000 INFO @ Tue, 02 Aug 2022 13:40:27: 4000000 INFO @ Tue, 02 Aug 2022 13:40:29: 1000000 INFO @ Tue, 02 Aug 2022 13:40:33: 8000000 INFO @ Tue, 02 Aug 2022 13:40:36: 5000000 INFO @ Tue, 02 Aug 2022 13:40:38: 2000000 INFO @ Tue, 02 Aug 2022 13:40:43: 9000000 INFO @ Tue, 02 Aug 2022 13:40:45: 6000000 INFO @ Tue, 02 Aug 2022 13:40:48: 3000000 INFO @ Tue, 02 Aug 2022 13:40:51: 10000000 INFO @ Tue, 02 Aug 2022 13:40:53: 7000000 INFO @ Tue, 02 Aug 2022 13:40:57: 4000000 INFO @ Tue, 02 Aug 2022 13:40:59: 11000000 INFO @ Tue, 02 Aug 2022 13:41:02: 8000000 INFO @ Tue, 02 Aug 2022 13:41:06: 5000000 INFO @ Tue, 02 Aug 2022 13:41:07: 12000000 INFO @ Tue, 02 Aug 2022 13:41:10: 9000000 INFO @ Tue, 02 Aug 2022 13:41:15: 6000000 INFO @ Tue, 02 Aug 2022 13:41:15: 13000000 INFO @ Tue, 02 Aug 2022 13:41:18: 10000000 INFO @ Tue, 02 Aug 2022 13:41:23: 14000000 INFO @ Tue, 02 Aug 2022 13:41:23: 7000000 INFO @ Tue, 02 Aug 2022 13:41:26: 11000000 INFO @ Tue, 02 Aug 2022 13:41:31: 15000000 INFO @ Tue, 02 Aug 2022 13:41:32: 8000000 INFO @ Tue, 02 Aug 2022 13:41:34: 12000000 INFO @ Tue, 02 Aug 2022 13:41:39: 16000000 INFO @ Tue, 02 Aug 2022 13:41:40: 9000000 INFO @ Tue, 02 Aug 2022 13:41:42: 13000000 INFO @ Tue, 02 Aug 2022 13:41:47: 17000000 INFO @ Tue, 02 Aug 2022 13:41:48: 10000000 INFO @ Tue, 02 Aug 2022 13:41:50: 14000000 INFO @ Tue, 02 Aug 2022 13:41:55: 18000000 INFO @ Tue, 02 Aug 2022 13:41:56: 11000000 INFO @ Tue, 02 Aug 2022 13:41:57: 15000000 INFO @ Tue, 02 Aug 2022 13:42:02: 19000000 INFO @ Tue, 02 Aug 2022 13:42:04: 12000000 INFO @ Tue, 02 Aug 2022 13:42:05: 16000000 INFO @ Tue, 02 Aug 2022 13:42:10: 20000000 INFO @ Tue, 02 Aug 2022 13:42:12: 13000000 INFO @ Tue, 02 Aug 2022 13:42:13: 17000000 INFO @ Tue, 02 Aug 2022 13:42:18: 21000000 INFO @ Tue, 02 Aug 2022 13:42:19: 14000000 INFO @ Tue, 02 Aug 2022 13:42:21: 18000000 INFO @ Tue, 02 Aug 2022 13:42:25: 22000000 INFO @ Tue, 02 Aug 2022 13:42:27: 15000000 INFO @ Tue, 02 Aug 2022 13:42:28: 19000000 INFO @ Tue, 02 Aug 2022 13:42:33: 23000000 INFO @ Tue, 02 Aug 2022 13:42:35: 16000000 INFO @ Tue, 02 Aug 2022 13:42:36: 20000000 INFO @ Tue, 02 Aug 2022 13:42:41: 24000000 INFO @ Tue, 02 Aug 2022 13:42:43: 17000000 INFO @ Tue, 02 Aug 2022 13:42:44: 21000000 INFO @ Tue, 02 Aug 2022 13:42:48: 25000000 INFO @ Tue, 02 Aug 2022 13:42:50: 18000000 INFO @ Tue, 02 Aug 2022 13:42:51: 22000000 INFO @ Tue, 02 Aug 2022 13:42:56: 26000000 INFO @ Tue, 02 Aug 2022 13:42:58: 19000000 INFO @ Tue, 02 Aug 2022 13:42:59: 23000000 INFO @ Tue, 02 Aug 2022 13:43:04: 27000000 INFO @ Tue, 02 Aug 2022 13:43:06: 20000000 INFO @ Tue, 02 Aug 2022 13:43:07: 24000000 INFO @ Tue, 02 Aug 2022 13:43:11: 28000000 INFO @ Tue, 02 Aug 2022 13:43:13: 21000000 INFO @ Tue, 02 Aug 2022 13:43:14: 25000000 INFO @ Tue, 02 Aug 2022 13:43:19: 29000000 INFO @ Tue, 02 Aug 2022 13:43:21: 22000000 INFO @ Tue, 02 Aug 2022 13:43:22: 26000000 INFO @ Tue, 02 Aug 2022 13:43:27: 30000000 INFO @ Tue, 02 Aug 2022 13:43:29: 23000000 INFO @ Tue, 02 Aug 2022 13:43:29: 27000000 INFO @ Tue, 02 Aug 2022 13:43:34: 31000000 INFO @ Tue, 02 Aug 2022 13:43:36: 24000000 INFO @ Tue, 02 Aug 2022 13:43:37: 28000000 INFO @ Tue, 02 Aug 2022 13:43:42: 32000000 INFO @ Tue, 02 Aug 2022 13:43:44: 25000000 INFO @ Tue, 02 Aug 2022 13:43:45: 29000000 INFO @ Tue, 02 Aug 2022 13:43:50: 33000000 INFO @ Tue, 02 Aug 2022 13:43:52: 26000000 INFO @ Tue, 02 Aug 2022 13:43:52: 30000000 INFO @ Tue, 02 Aug 2022 13:43:58: 34000000 INFO @ Tue, 02 Aug 2022 13:44:00: 27000000 INFO @ Tue, 02 Aug 2022 13:44:00: 31000000 INFO @ Tue, 02 Aug 2022 13:44:05: 35000000 INFO @ Tue, 02 Aug 2022 13:44:07: 28000000 INFO @ Tue, 02 Aug 2022 13:44:08: 32000000 INFO @ Tue, 02 Aug 2022 13:44:13: 36000000 INFO @ Tue, 02 Aug 2022 13:44:15: 29000000 INFO @ Tue, 02 Aug 2022 13:44:15: 33000000 INFO @ Tue, 02 Aug 2022 13:44:21: 37000000 INFO @ Tue, 02 Aug 2022 13:44:23: 30000000 INFO @ Tue, 02 Aug 2022 13:44:23: 34000000 INFO @ Tue, 02 Aug 2022 13:44:28: 38000000 INFO @ Tue, 02 Aug 2022 13:44:30: 31000000 INFO @ Tue, 02 Aug 2022 13:44:31: 35000000 INFO @ Tue, 02 Aug 2022 13:44:36: 39000000 INFO @ Tue, 02 Aug 2022 13:44:38: 32000000 INFO @ Tue, 02 Aug 2022 13:44:38: 36000000 INFO @ Tue, 02 Aug 2022 13:44:44: 40000000 INFO @ Tue, 02 Aug 2022 13:44:46: 33000000 INFO @ Tue, 02 Aug 2022 13:44:46: 37000000 INFO @ Tue, 02 Aug 2022 13:44:52: 41000000 INFO @ Tue, 02 Aug 2022 13:44:53: 34000000 INFO @ Tue, 02 Aug 2022 13:44:53: 38000000 INFO @ Tue, 02 Aug 2022 13:44:59: 42000000 INFO @ Tue, 02 Aug 2022 13:45:01: 35000000 INFO @ Tue, 02 Aug 2022 13:45:01: 39000000 INFO @ Tue, 02 Aug 2022 13:45:07: 43000000 INFO @ Tue, 02 Aug 2022 13:45:08: 36000000 INFO @ Tue, 02 Aug 2022 13:45:09: 40000000 INFO @ Tue, 02 Aug 2022 13:45:15: 44000000 INFO @ Tue, 02 Aug 2022 13:45:16: 37000000 INFO @ Tue, 02 Aug 2022 13:45:16: 41000000 INFO @ Tue, 02 Aug 2022 13:45:22: 45000000 INFO @ Tue, 02 Aug 2022 13:45:24: 38000000 INFO @ Tue, 02 Aug 2022 13:45:24: 42000000 INFO @ Tue, 02 Aug 2022 13:45:30: 46000000 INFO @ Tue, 02 Aug 2022 13:45:31: 39000000 INFO @ Tue, 02 Aug 2022 13:45:32: 43000000 INFO @ Tue, 02 Aug 2022 13:45:38: 47000000 INFO @ Tue, 02 Aug 2022 13:45:39: 40000000 INFO @ Tue, 02 Aug 2022 13:45:39: 44000000 INFO @ Tue, 02 Aug 2022 13:45:45: 48000000 INFO @ Tue, 02 Aug 2022 13:45:47: 41000000 INFO @ Tue, 02 Aug 2022 13:45:47: 45000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:45:53: 49000000 INFO @ Tue, 02 Aug 2022 13:45:54: 42000000 INFO @ Tue, 02 Aug 2022 13:45:55: 46000000 INFO @ Tue, 02 Aug 2022 13:46:01: 50000000 INFO @ Tue, 02 Aug 2022 13:46:02: 43000000 INFO @ Tue, 02 Aug 2022 13:46:02: 47000000 INFO @ Tue, 02 Aug 2022 13:46:09: 51000000 INFO @ Tue, 02 Aug 2022 13:46:10: 44000000 INFO @ Tue, 02 Aug 2022 13:46:10: 48000000 INFO @ Tue, 02 Aug 2022 13:46:17: 52000000 INFO @ Tue, 02 Aug 2022 13:46:18: 45000000 INFO @ Tue, 02 Aug 2022 13:46:18: 49000000 INFO @ Tue, 02 Aug 2022 13:46:24: 53000000 INFO @ Tue, 02 Aug 2022 13:46:25: 50000000 INFO @ Tue, 02 Aug 2022 13:46:25: 46000000 INFO @ Tue, 02 Aug 2022 13:46:32: 54000000 INFO @ Tue, 02 Aug 2022 13:46:33: 51000000 INFO @ Tue, 02 Aug 2022 13:46:33: 47000000 INFO @ Tue, 02 Aug 2022 13:46:40: 55000000 INFO @ Tue, 02 Aug 2022 13:46:41: 52000000 INFO @ Tue, 02 Aug 2022 13:46:41: 48000000 INFO @ Tue, 02 Aug 2022 13:46:47: 56000000 INFO @ Tue, 02 Aug 2022 13:46:48: 53000000 INFO @ Tue, 02 Aug 2022 13:46:49: 49000000 INFO @ Tue, 02 Aug 2022 13:46:55: 57000000 INFO @ Tue, 02 Aug 2022 13:46:56: 54000000 INFO @ Tue, 02 Aug 2022 13:46:56: 50000000 INFO @ Tue, 02 Aug 2022 13:47:03: 58000000 INFO @ Tue, 02 Aug 2022 13:47:03: 55000000 INFO @ Tue, 02 Aug 2022 13:47:04: 51000000 INFO @ Tue, 02 Aug 2022 13:47:11: 59000000 INFO @ Tue, 02 Aug 2022 13:47:11: 56000000 INFO @ Tue, 02 Aug 2022 13:47:12: 52000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 13:47:19: 60000000 INFO @ Tue, 02 Aug 2022 13:47:19: 57000000 INFO @ Tue, 02 Aug 2022 13:47:20: 53000000 INFO @ Tue, 02 Aug 2022 13:47:26: 61000000 INFO @ Tue, 02 Aug 2022 13:47:26: 58000000 INFO @ Tue, 02 Aug 2022 13:47:27: 54000000 INFO @ Tue, 02 Aug 2022 13:47:34: 59000000 INFO @ Tue, 02 Aug 2022 13:47:34: 62000000 INFO @ Tue, 02 Aug 2022 13:47:35: 55000000 INFO @ Tue, 02 Aug 2022 13:47:42: 60000000 INFO @ Tue, 02 Aug 2022 13:47:42: 63000000 INFO @ Tue, 02 Aug 2022 13:47:43: 56000000 INFO @ Tue, 02 Aug 2022 13:47:50: 61000000 INFO @ Tue, 02 Aug 2022 13:47:50: 64000000 INFO @ Tue, 02 Aug 2022 13:47:51: 57000000 INFO @ Tue, 02 Aug 2022 13:47:57: 62000000 INFO @ Tue, 02 Aug 2022 13:47:58: 65000000 INFO @ Tue, 02 Aug 2022 13:47:59: 58000000 INFO @ Tue, 02 Aug 2022 13:48:05: 63000000 INFO @ Tue, 02 Aug 2022 13:48:06: 66000000 INFO @ Tue, 02 Aug 2022 13:48:06: 59000000 INFO @ Tue, 02 Aug 2022 13:48:13: 64000000 INFO @ Tue, 02 Aug 2022 13:48:14: 67000000 INFO @ Tue, 02 Aug 2022 13:48:14: 60000000 INFO @ Tue, 02 Aug 2022 13:48:21: 65000000 INFO @ Tue, 02 Aug 2022 13:48:21: 68000000 INFO @ Tue, 02 Aug 2022 13:48:22: 61000000 INFO @ Tue, 02 Aug 2022 13:48:29: 66000000 INFO @ Tue, 02 Aug 2022 13:48:30: 69000000 INFO @ Tue, 02 Aug 2022 13:48:30: 62000000 INFO @ Tue, 02 Aug 2022 13:48:33: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 13:48:33: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 13:48:33: #1 total tags in treatment: 32507813 INFO @ Tue, 02 Aug 2022 13:48:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:48:34: #1 tags after filtering in treatment: 27988259 INFO @ Tue, 02 Aug 2022 13:48:34: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 13:48:34: #1 finished! INFO @ Tue, 02 Aug 2022 13:48:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:48:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:48:35: #2 number of paired peaks: 135 WARNING @ Tue, 02 Aug 2022 13:48:35: Fewer paired peaks (135) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 135 pairs to build model! INFO @ Tue, 02 Aug 2022 13:48:35: start model_add_line... INFO @ Tue, 02 Aug 2022 13:48:36: start X-correlation... INFO @ Tue, 02 Aug 2022 13:48:36: end of X-cor INFO @ Tue, 02 Aug 2022 13:48:36: #2 finished! INFO @ Tue, 02 Aug 2022 13:48:36: #2 predicted fragment length is 166 bps INFO @ Tue, 02 Aug 2022 13:48:36: #2 alternative fragment length(s) may be 3,166 bps INFO @ Tue, 02 Aug 2022 13:48:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.05_model.r WARNING @ Tue, 02 Aug 2022 13:48:36: #2 Since the d (166) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 13:48:36: #2 You may need to consider one of the other alternative d(s): 3,166 WARNING @ Tue, 02 Aug 2022 13:48:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 13:48:36: #3 Call peaks... INFO @ Tue, 02 Aug 2022 13:48:36: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 13:48:37: 67000000 INFO @ Tue, 02 Aug 2022 13:48:38: 63000000 INFO @ Tue, 02 Aug 2022 13:48:45: 68000000 INFO @ Tue, 02 Aug 2022 13:48:46: 64000000 INFO @ Tue, 02 Aug 2022 13:48:53: 69000000 INFO @ Tue, 02 Aug 2022 13:48:54: 65000000 INFO @ Tue, 02 Aug 2022 13:48:56: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 13:48:56: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 13:48:56: #1 total tags in treatment: 32507813 INFO @ Tue, 02 Aug 2022 13:48:56: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:48:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:48:57: #1 tags after filtering in treatment: 27988259 INFO @ Tue, 02 Aug 2022 13:48:57: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 13:48:57: #1 finished! INFO @ Tue, 02 Aug 2022 13:48:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:48:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:48:59: #2 number of paired peaks: 135 WARNING @ Tue, 02 Aug 2022 13:48:59: Fewer paired peaks (135) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 135 pairs to build model! INFO @ Tue, 02 Aug 2022 13:48:59: start model_add_line... INFO @ Tue, 02 Aug 2022 13:48:59: start X-correlation... INFO @ Tue, 02 Aug 2022 13:48:59: end of X-cor INFO @ Tue, 02 Aug 2022 13:48:59: #2 finished! INFO @ Tue, 02 Aug 2022 13:48:59: #2 predicted fragment length is 166 bps INFO @ Tue, 02 Aug 2022 13:48:59: #2 alternative fragment length(s) may be 3,166 bps INFO @ Tue, 02 Aug 2022 13:48:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.10_model.r WARNING @ Tue, 02 Aug 2022 13:48:59: #2 Since the d (166) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 13:48:59: #2 You may need to consider one of the other alternative d(s): 3,166 WARNING @ Tue, 02 Aug 2022 13:48:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 13:48:59: #3 Call peaks... INFO @ Tue, 02 Aug 2022 13:48:59: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 13:49:01: 66000000 INFO @ Tue, 02 Aug 2022 13:49:08: 67000000 INFO @ Tue, 02 Aug 2022 13:49:15: 68000000 INFO @ Tue, 02 Aug 2022 13:49:22: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 13:49:23: 69000000 INFO @ Tue, 02 Aug 2022 13:49:26: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 13:49:26: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 13:49:26: #1 total tags in treatment: 32507813 INFO @ Tue, 02 Aug 2022 13:49:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:49:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:49:26: #1 tags after filtering in treatment: 27988259 INFO @ Tue, 02 Aug 2022 13:49:26: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 13:49:26: #1 finished! INFO @ Tue, 02 Aug 2022 13:49:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:49:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:49:28: #2 number of paired peaks: 135 WARNING @ Tue, 02 Aug 2022 13:49:28: Fewer paired peaks (135) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 135 pairs to build model! INFO @ Tue, 02 Aug 2022 13:49:28: start model_add_line... INFO @ Tue, 02 Aug 2022 13:49:28: start X-correlation... INFO @ Tue, 02 Aug 2022 13:49:28: end of X-cor INFO @ Tue, 02 Aug 2022 13:49:28: #2 finished! INFO @ Tue, 02 Aug 2022 13:49:28: #2 predicted fragment length is 166 bps INFO @ Tue, 02 Aug 2022 13:49:28: #2 alternative fragment length(s) may be 3,166 bps INFO @ Tue, 02 Aug 2022 13:49:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.20_model.r WARNING @ Tue, 02 Aug 2022 13:49:29: #2 Since the d (166) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 13:49:29: #2 You may need to consider one of the other alternative d(s): 3,166 WARNING @ Tue, 02 Aug 2022 13:49:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 13:49:29: #3 Call peaks... INFO @ Tue, 02 Aug 2022 13:49:29: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 13:49:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.05_peaks.xls INFO @ Tue, 02 Aug 2022 13:49:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 13:49:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.05_summits.bed INFO @ Tue, 02 Aug 2022 13:49:44: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2983 records, 4 fields): 130 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:49:46: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 13:50:06: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.10_peaks.xls INFO @ Tue, 02 Aug 2022 13:50:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 13:50:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.10_summits.bed INFO @ Tue, 02 Aug 2022 13:50:07: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1815 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:50:15: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 13:50:36: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.20_peaks.xls INFO @ Tue, 02 Aug 2022 13:50:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 13:50:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX12572570/SRX12572570.20_summits.bed INFO @ Tue, 02 Aug 2022 13:50:36: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (689 records, 4 fields): 47 millis CompletedMACS2peakCalling