Job ID = 16434895 SRX = SRX11633405 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:51 27402976 reads; of these: 27402976 (100.00%) were unpaired; of these: 1534593 (5.60%) aligned 0 times 22065803 (80.52%) aligned exactly 1 time 3802580 (13.88%) aligned >1 times 94.40% overall alignment rate Time searching: 00:08:51 Overall time: 00:08:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2771630 / 25868383 = 0.1071 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:31:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:31:39: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:31:39: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:31:45: 1000000 INFO @ Tue, 02 Aug 2022 10:31:52: 2000000 INFO @ Tue, 02 Aug 2022 10:31:57: 3000000 INFO @ Tue, 02 Aug 2022 10:32:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:32:09: 5000000 INFO @ Tue, 02 Aug 2022 10:32:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:32:09: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:32:09: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:32:15: 6000000 INFO @ Tue, 02 Aug 2022 10:32:16: 1000000 INFO @ Tue, 02 Aug 2022 10:32:21: 7000000 INFO @ Tue, 02 Aug 2022 10:32:22: 2000000 INFO @ Tue, 02 Aug 2022 10:32:27: 8000000 INFO @ Tue, 02 Aug 2022 10:32:28: 3000000 INFO @ Tue, 02 Aug 2022 10:32:33: 9000000 INFO @ Tue, 02 Aug 2022 10:32:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:32:39: 10000000 INFO @ Tue, 02 Aug 2022 10:32:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:32:39: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:32:39: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:32:41: 5000000 INFO @ Tue, 02 Aug 2022 10:32:46: 11000000 INFO @ Tue, 02 Aug 2022 10:32:46: 1000000 INFO @ Tue, 02 Aug 2022 10:32:48: 6000000 INFO @ Tue, 02 Aug 2022 10:32:52: 12000000 INFO @ Tue, 02 Aug 2022 10:32:53: 2000000 INFO @ Tue, 02 Aug 2022 10:32:54: 7000000 INFO @ Tue, 02 Aug 2022 10:32:58: 13000000 INFO @ Tue, 02 Aug 2022 10:33:00: 3000000 INFO @ Tue, 02 Aug 2022 10:33:01: 8000000 INFO @ Tue, 02 Aug 2022 10:33:04: 14000000 INFO @ Tue, 02 Aug 2022 10:33:06: 4000000 INFO @ Tue, 02 Aug 2022 10:33:07: 9000000 INFO @ Tue, 02 Aug 2022 10:33:10: 15000000 INFO @ Tue, 02 Aug 2022 10:33:13: 5000000 INFO @ Tue, 02 Aug 2022 10:33:13: 10000000 INFO @ Tue, 02 Aug 2022 10:33:16: 16000000 INFO @ Tue, 02 Aug 2022 10:33:19: 6000000 INFO @ Tue, 02 Aug 2022 10:33:19: 11000000 INFO @ Tue, 02 Aug 2022 10:33:22: 17000000 INFO @ Tue, 02 Aug 2022 10:33:25: 12000000 INFO @ Tue, 02 Aug 2022 10:33:25: 7000000 INFO @ Tue, 02 Aug 2022 10:33:28: 18000000 INFO @ Tue, 02 Aug 2022 10:33:31: 13000000 INFO @ Tue, 02 Aug 2022 10:33:32: 8000000 INFO @ Tue, 02 Aug 2022 10:33:34: 19000000 INFO @ Tue, 02 Aug 2022 10:33:37: 14000000 INFO @ Tue, 02 Aug 2022 10:33:38: 9000000 INFO @ Tue, 02 Aug 2022 10:33:40: 20000000 INFO @ Tue, 02 Aug 2022 10:33:43: 15000000 INFO @ Tue, 02 Aug 2022 10:33:44: 10000000 INFO @ Tue, 02 Aug 2022 10:33:46: 21000000 INFO @ Tue, 02 Aug 2022 10:33:49: 16000000 INFO @ Tue, 02 Aug 2022 10:33:50: 11000000 INFO @ Tue, 02 Aug 2022 10:33:52: 22000000 INFO @ Tue, 02 Aug 2022 10:33:55: 17000000 INFO @ Tue, 02 Aug 2022 10:33:56: 12000000 INFO @ Tue, 02 Aug 2022 10:33:58: 23000000 INFO @ Tue, 02 Aug 2022 10:33:59: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 10:33:59: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 10:33:59: #1 total tags in treatment: 23096753 INFO @ Tue, 02 Aug 2022 10:33:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:33:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:33:59: #1 tags after filtering in treatment: 23096753 INFO @ Tue, 02 Aug 2022 10:33:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:33:59: #1 finished! INFO @ Tue, 02 Aug 2022 10:33:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:33:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:34:01: #2 number of paired peaks: 86 WARNING @ Tue, 02 Aug 2022 10:34:01: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:34:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 10:34:01: 18000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:34:02: 13000000 INFO @ Tue, 02 Aug 2022 10:34:07: 19000000 INFO @ Tue, 02 Aug 2022 10:34:07: 14000000 INFO @ Tue, 02 Aug 2022 10:34:13: 20000000 INFO @ Tue, 02 Aug 2022 10:34:13: 15000000 INFO @ Tue, 02 Aug 2022 10:34:19: 21000000 INFO @ Tue, 02 Aug 2022 10:34:19: 16000000 INFO @ Tue, 02 Aug 2022 10:34:24: 22000000 INFO @ Tue, 02 Aug 2022 10:34:25: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 10:34:30: 23000000 INFO @ Tue, 02 Aug 2022 10:34:31: 18000000 INFO @ Tue, 02 Aug 2022 10:34:31: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 10:34:31: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 10:34:31: #1 total tags in treatment: 23096753 INFO @ Tue, 02 Aug 2022 10:34:31: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:34:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:34:31: #1 tags after filtering in treatment: 23096753 INFO @ Tue, 02 Aug 2022 10:34:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:34:31: #1 finished! INFO @ Tue, 02 Aug 2022 10:34:31: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:34:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:34:33: #2 number of paired peaks: 86 WARNING @ Tue, 02 Aug 2022 10:34:33: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:34:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:34:36: 19000000 INFO @ Tue, 02 Aug 2022 10:34:42: 20000000 INFO @ Tue, 02 Aug 2022 10:34:48: 21000000 INFO @ Tue, 02 Aug 2022 10:34:53: 22000000 INFO @ Tue, 02 Aug 2022 10:34:59: 23000000 INFO @ Tue, 02 Aug 2022 10:35:00: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 10:35:00: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 10:35:00: #1 total tags in treatment: 23096753 INFO @ Tue, 02 Aug 2022 10:35:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:35:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:35:00: #1 tags after filtering in treatment: 23096753 INFO @ Tue, 02 Aug 2022 10:35:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:35:00: #1 finished! INFO @ Tue, 02 Aug 2022 10:35:00: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:35:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:35:01: #2 number of paired peaks: 86 WARNING @ Tue, 02 Aug 2022 10:35:01: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:35:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633405/SRX11633405.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。